FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGT511.627906976744185No Hit
ATGTAAACCTGGCTGTTATTGGAGTCCTCTGACCAAGAGATAATAGTGAA24.651162790697675No Hit
CAATCACCACGACGATGAGGACCACCACCACCACCACGATAAGAAGGCGT24.651162790697675No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24.651162790697675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24.651162790697675Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTGAACAGGCCACCGACCATGACCGCCGACGACCGACAACCCGCCTT24.651162790697675No Hit
GACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAAC24.651162790697675No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GGACATTGAAAGCGCCCTCGGAGAGATTCATCGTCAAGAGGGGGGGCGGC12.3255813953488373No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT12.3255813953488373No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG12.3255813953488373No Hit
CAAAAGCCGATTAGCTCGCCAAACCCTTCGCGGGCATAGGTAAAGATACC12.3255813953488373No Hit
GGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC12.3255813953488373No Hit
GGATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG12.3255813953488373No Hit
GACATGGCTGGCTCCTGGTTGCTCATAATGGCGGAGATCCGGATGCAAAC12.3255813953488373No Hit
GGGCTCGGGCAAGTCCACGCTGCTGCGCGCCATCAACCGCCTGGAGCCGC12.3255813953488373No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCT12.3255813953488373No Hit
GTGCGAACTGGCCTTTCTCCACACCAGCCACTGCCTGGCCAGCGGGGAAG12.3255813953488373No Hit
GATGGACACAGCTCGGACCAAGTCTTTCCGAGGTGGGTATTTGGGATGTT12.3255813953488373No Hit
TTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGT12.3255813953488373No Hit
CCCCAGGCCCTTGGAAAGTGCACTTGGGCACAGGGGCAATGGCATCAGCT12.3255813953488373No Hit
GTCATGTTCTGTTACCAGGAAGACGATATCTTTGGCCCAATAAATCTGCC12.3255813953488373No Hit
TCTTTGTCACATTGGGGCACAATGCTTCCCACAGGCCTGGCCTAGGCATA12.3255813953488373No Hit
CCACAAGACGCTTTTCTCCACAAGCCCTGTCATTCCACTTGCCATTGGTG12.3255813953488373No Hit
GTCCCCCAACGCCTGCAGCTGCACGCCGCACTCGGCCCACAGCATGCCCT12.3255813953488373No Hit
GACCCTCAAAATAAACAGCCTCTACCTTGCGAGCCGTCTTCCCCAGGCCT12.3255813953488373No Hit
GGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAG12.3255813953488373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGT12.3255813953488373Clontech Universal Primer Mix Long (95% over 22bp)
GCTCCAAACACTCATGTTTTTGTTTCTTTCTTCTCCTCATCCAACTGGCT12.3255813953488373No Hit
GTTTGAATAAACCCCAAAACAAAAAAGGCGAGTTTTAAATCTGTCTCTTA12.3255813953488373No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAACGGGGTTTA12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)