FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62.2641509433962264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.509433962264151Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41.509433962264151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTGGGCCTGCCCTCGCGGCTTACCGACTGGAAGACACGTTTGGCCCAG31.1320754716981132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA31.1320754716981132Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGTTAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA31.1320754716981132Illumina Single End PCR Primer 1 (100% over 41bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
GAGCATCCAAGGTTCTAGTCTCTGTTGCTGTGCTGGTCTTTAGCCCCACT31.1320754716981132No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.1320754716981132Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATTTATGAAGCCTTAAGATCCAGAAGTGTTGTTACTACCAAACCTCTGA20.7547169811320755No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG20.7547169811320755Illumina Single End PCR Primer 1 (100% over 22bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAA20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGGGAACGGCATTCAGCCTGCAAGTGGGACAGTTGCAAGGCTTTGTT20.7547169811320755No Hit
ATTCTTGTCTGCCAGCTCCGGGTGCTTAGGCATGTGGACATCCTTCTTGG20.7547169811320755No Hit
CCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGGCG20.7547169811320755No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCATGTACT20.7547169811320755TruSeq Adapter, Index 6 (97% over 37bp)
GTACAGTAGAAAGAGGAGGAAGAGGAGGAGAAAGAGAATAGTGTAGGAAG20.7547169811320755No Hit
CACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGTGTAG20.7547169811320755No Hit
GAGTACATGGGAAGCAGTTGTATCAACGCAGAGTACATGGGAAGCAGTGT20.7547169811320755Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTATAGATGTTTATTAGTTTTTAGATTTAAAAAAAAAAACAGGGCTTA20.7547169811320755No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.7547169811320755Clontech SMART CDS Primer II A (100% over 26bp)
GCGCCAGCGGACGCTGCGCTTCCGAGCCGATGCTGTGCGAGAGCGCCGCC20.7547169811320755No Hit
CAGTAGTATGAATGTTCCTCTTTCTCCAAATGCTGAAGAGTAACAAATCC20.7547169811320755No Hit
CTTCCAGTCTGAATATCATATTGGCATCTTTTCCTCTTTGTGCTTTAGGT20.7547169811320755No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.7547169811320755Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.7547169811320755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAGAAGGTACTGCTTCTTGTCACCACACTGAGCTCTGAATTTGTATTAC20.7547169811320755No Hit
TATCAACGCAGAGTACATGGGAAGCGCAGTGGTATCAACGCAGAGTACAT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC10.37735849056603776No Hit
NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCAAAGG10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATAAATTATCTTAAA10.37735849056603776No Hit
GAGTACATGGGAATGATCGGCGACCACCGAGATCTACACTCTTTCTGTCT10.37735849056603776Illumina Single End PCR Primer 1 (96% over 28bp)
GGGCACGGTGGCTCATGCCTGTAATCCCAGTACTTTGAGAGGCTGAGGTG10.37735849056603776No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGGGACAGAGAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTC10.37735849056603776Illumina Single End PCR Primer 1 (96% over 30bp)
TATCAACGCAGAGTACATGGGTGTACGGCGACCACCCAGATCTACACACT10.37735849056603776No Hit
GAAAGAATGTGCACTGCATTTTCACCCCTCCCCTCTCACTGCTGCCTCAG10.37735849056603776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACTATGGAGTTTAAACAAC10.37735849056603776No Hit
CAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 23bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGTTTATACCACTG10.37735849056603776No Hit
GATGAGATTGGCATGGCTTTATTTGTTTTTTTTTGTTTTGTTTTGGTTTT10.37735849056603776No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 22bp)
GTTTCGCCCTCTTCCTTCTGGTTTCCTATGGAAAAGTCATGGGTGTGTGA10.37735849056603776No Hit
TCTTTGCCCACTGCAACCTCTGCCTTCTGGGTTCAAGTGATTCTCCTGCC10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCTGCT10.37735849056603776No Hit
CTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGACGGAAGAGC10.37735849056603776No Hit
CTTCCGATCTCATGATGCAAGGACTGGAGTCGGAAGAGCGTCGTGTAGGG10.37735849056603776Illumina Single End PCR Primer 1 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTGCGTTTTTACCACTGCTTCCCATGTACCT10.37735849056603776No Hit
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAG10.37735849056603776Illumina Single End PCR Primer 1 (96% over 25bp)
CGCTGTATCTGCAAATGAACAGTCTGAGAGCCGAGGACACGGCTGTGTAT10.37735849056603776No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATAA10.37735849056603776No Hit
GAGTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA10.37735849056603776Illumina Single End PCR Primer 1 (100% over 39bp)
CTACACGACGCTCTTCCGATCTCTGGTACCTGTCCCAAGTTCTGCAGGTA10.37735849056603776Illumina Single End PCR Primer 1 (100% over 22bp)
GGTATCAACGCAGAGTACATGGGACACCCGAGATCTACATCTTTCCCTAC10.37735849056603776No Hit
GATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTC10.37735849056603776No Hit
GTACATGGGGCAGTGGTATCAACGGCAAAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCTGT10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGATGAACGCCACCGAGATCTACACTCTTT10.37735849056603776Illumina Single End PCR Primer 1 (100% over 21bp)
TCCAAAGGCTGGGAGGAAAGAACCCAAACCCAGGCCCTCCTCCTGCCCTC10.37735849056603776No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAATGTATCT10.37735849056603776No Hit
CCAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGTAGATCCGGTGG10.37735849056603776Illumina Single End PCR Primer 1 (96% over 30bp)
AGTAAATACTTTGTTCCTGGAACTGCGATTTTAGCACATTATACTGAGCA10.37735849056603776No Hit
GAACATATTCAAACAGTAAAGGAGCTCCAACTATTGAACGTTAGCATCAA10.37735849056603776No Hit
GGATGATACGGCGACCACCGAGATCTACACTCTTTCCCAACACGACGCTC10.37735849056603776Illumina Single End PCR Primer 1 (97% over 37bp)
GAAGAGCGTCGTGTAGAAGAGTGTAGATCCGGTTGGCGTCACCCATGTAC10.37735849056603776No Hit
AATTAGCATATAGAAAAATATTACTTTAAGATGACTTGTTTCCTTTGAAA10.37735849056603776No Hit
GAATACTACTGGGAGAAAGATTCTAAAAAGCAGTCACAGAAAAGGTGGTA10.37735849056603776No Hit
TTATCGCGCTGCTGGCTTCTCCCCAGCTGGCGCTGTTCTTTGAAAAATTT10.37735849056603776No Hit
ATTACAACATGCTTACAAATTAGAACATGTCAGCCGAAACAAAGTACATT10.37735849056603776No Hit
ATGCAGGCCTTTTCGGCCTCCGCATAACCTTCGCTCTTCTCCTCACGCTT10.37735849056603776No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACCCTGCGTTGATACCTGTCTCTTATACACA10.37735849056603776No Hit
CCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGT10.37735849056603776No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
AATGGAATCATTGGATGTACTCGAATGAAACCATCGAATGGACTCGAATG10.37735849056603776No Hit
GCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACTGGCTT10.37735849056603776No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA10.37735849056603776No Hit
CCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGG10.37735849056603776Illumina Single End PCR Primer 1 (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.37735849056603776Clontech Universal Primer Mix Long (100% over 23bp)
ACCATACCCTGGGGATTACTCTGAACTTTGTTCATTTGTTTTCTTTTAAT10.37735849056603776No Hit
GGAGTGCAGTGGAACGATCTCAGCTCACTGCAACGTCTGTCTCCCAAGTA10.37735849056603776No Hit
GTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACTGTCTCTT10.37735849056603776No Hit
CATTTCAGGAAGATCCCCTGCGCGTACTGCGCGTGGCGCGCTTTGCCGCA10.37735849056603776No Hit
AATTATGATTATAAACATGGTTATATCAGTCTTGTATTTGAAAAAATTGT10.37735849056603776No Hit
GCCTGGATCGATGACTGGAATTTCGCCAGCCGCCCCGGTGCCACCCGCGC10.37735849056603776No Hit
ATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGAC10.37735849056603776No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGGGTTTTAAAC10.37735849056603776No Hit
CCGCTATTTGTTACCATGATACATTCCCAAGTCAGATTTACAGTTTGTTT10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACGGGTTCCCATTT10.37735849056603776No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACACA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 22bp)
CACTGGAAGACACTCAGCAATTAATGAAGTTAATTACTGGGCCAACTTGA10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAATTTTTTATTTT10.37735849056603776No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTAT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTGATTTTTTTCCCCCCAGAACCAATGCAGGAACTTAACAGAAAAACCTA10.37735849056603776No Hit
AGTGTAGCCTAAGTATAAAATATTTATGAAGTCGACAGTAGTGTACAGGA10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAAATGATACC10.37735849056603776No Hit
GGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTC10.37735849056603776No Hit
CTCCAGCCTGGGCAACAGAGTGAGACTCCGTCTCAAAAAAAAAAAAAAAA10.37735849056603776No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTTTAAC10.37735849056603776No Hit
CAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTACTGGATCAGTCTCTAGCTGGAATGATGCCAATTGTGACAGAGGGCAG10.37735849056603776No Hit
AAATGGAACAAATGACTATCCACATACAAGAGAATACTGGAGCCTTACAC10.37735849056603776No Hit
CAGTTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.37735849056603776Clontech Universal Primer Mix Long (100% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCAGCGTTTAAACCCA10.37735849056603776No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGTACGGCGACCCCCCAGAACAAAA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGGAAAGAGTGTAGATCGGTGGCCCTACACCCATGTACCTGTCTCTTATA10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTAGTTTAAAACAAAGAATACC10.37735849056603776No Hit
TTTCCCAATACTGTGTAAGTGAGTGACAGAACTGTAGTTTTGGGCAACAG10.37735849056603776No Hit
CCTCAAGGATCTAGAACTAGAAATACCATTTGACCCAGCAATCCCATTAC10.37735849056603776No Hit
CGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGCGTCGTGTAG10.37735849056603776No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT10.37735849056603776Clontech SMART CDS Primer II A (100% over 24bp)
ATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGCTGTCTCTTAT10.37735849056603776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTTATACCACTG10.37735849056603776No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAGGGCGTCGCCGTCCGTGTCGCGGGCCATGGCGCCCAGGCTGACGC10.37735849056603776No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGCTCTGGGCAAGTTACAGAGCTCCTCCATGCCTCAGTTTCCTCAG10.37735849056603776No Hit
CGCCTGAGAATCACGCATGTTCACTTGGAAAATACCCCATTTCCCCAGAA10.37735849056603776No Hit
TATCAACGCAGTGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
CCTAGGTATTAAGCCCAGCAAGCATTAGCTACTTTTCCATAAAAAGAAAC10.37735849056603776No Hit
GGGTGGGGCCTCATGATGGGATTAGAGCCTTTATAAGAAGAAACTCGGGT10.37735849056603776No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.37735849056603776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAT10.37735849056603776No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGC10.37735849056603776No Hit
CATTAAAAATATTTGAGGTTTATTTATTTTTTTTTTTAGTTTTTTTTTTT10.37735849056603776No Hit
ACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGCATGAGATCG10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGACTGTCTCTTAT10.37735849056603776Clontech SMART CDS Primer II A (100% over 26bp)
CTCGTGTCGCGTATCACCCATGTACTCTGCCTGTCTCTTATACACATCTG10.37735849056603776No Hit
TACGAATGCCCCCGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCA10.37735849056603776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCG10.37735849056603776No Hit
GTATCAACGCAGAGTACATGGGTGTACGGCGACCACCGAGATCTACACTC10.37735849056603776Illumina Single End PCR Primer 1 (96% over 27bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTCTGTCTCTT10.37735849056603776Illumina Single End PCR Primer 1 (97% over 41bp)
TCTAGAGAGCAATCCTAGCACAATAATGTTTCAACTTGCAAGGAAGAACG10.37735849056603776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.37735849056603776No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGCGACAGAGATACACTTTCCCTACACAGCAACTCCAGTCC10.37735849056603776No Hit
ATCTACTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGTCCCAAGT10.37735849056603776Illumina Single End PCR Primer 1 (100% over 26bp)
CAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA10.37735849056603776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.37735849056603776No Hit
GTGATTCATGTAGAGAGACAGGGGAGTTGTCAACTTGGGCTACTGAACTA10.37735849056603776No Hit
GCCCAGATGGCGGTGCTGCATGCATCGGCCGAGGGGGCGATCGCGGCGCT10.37735849056603776No Hit
TAATCGGAGATGTGTATAAGAGACAGTATCAACGCAGAGTACATGGGAAG10.37735849056603776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.37735849056603776No Hit
GCCTTGGGGAGAACGACCCGCTGCGCACCTGGGGATGGGACCGACGAGAA10.37735849056603776No Hit
CGCCGATGGTGCCGCGCGAGGGCGGCGTGCCGGCCACGCCCGAGATGACG10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGAAACCACTGCTTCCCA10.37735849056603776No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.37735849056603776No Hit
CTTCAGATTTCCCTGAATATTTTCCATCCCGACAATATGCCTCTTAGATA10.37735849056603776No Hit
CCTCCCAACGGTTCTAACTTCTTCCTGTTACCCAGTTCCAAAGTCACTTC10.37735849056603776No Hit
GATATAGTTTGGCTGTGTCCCCACCCAAAGCTCATCTTGAGTTGTAGCTC10.37735849056603776No Hit
ACACTTGCCTCAGATTCTGTGTACCAGAGATCGGAAGAGCGTCGTGTAGG10.37735849056603776Illumina Single End PCR Primer 1 (100% over 23bp)
GAGGAAATCTAAATTGCACAAAATGTTTCAAGAAAAATATTTTTTAAGTC10.37735849056603776No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GATCAAGAGACTGAGACCATCCTGGTCAACATGGTGAAACCCATCTCTAC10.37735849056603776No Hit
TCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCCTGTCTCTTATACAC10.37735849056603776Illumina Single End PCR Primer 1 (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGATACGGCGACCACCGAGATCTACATC10.37735849056603776Illumina Single End PCR Primer 1 (96% over 25bp)
GTGCTAAATCTATCTGCAAACTGACAAACTAATCAGTTTCTGTAATTGTA10.37735849056603776No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.37735849056603776No Hit
CAATAACTATCCAACTCTCCAGATAAATGGACCAAAGAAATAAACAGACC10.37735849056603776No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.37735849056603776Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.37735849056603776Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.37735849056603776Clontech Universal Primer Mix Long (96% over 25bp)
AGAAGAATCAATACCACCTGGAGGCTCATTAGAACCGGCAGATTTACCGG10.37735849056603776No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10.37735849056603776No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTCTGTCTCTTATACACATCT10.37735849056603776No Hit
CTCTTGGGTTGTGGTGACTTGGGGAAGCTCTTGGGAGGGACCTAGAGGCT10.37735849056603776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTGTACGGCGACCACCCAGATCAACACACTTTACCAACACGA10.37735849056603776No Hit
GTATCAACGCAGAGTACATGGGAGAGGAGAAGCCAAAGCGAGGGAGCATT10.37735849056603776No Hit
TGTCTACCTGATGGATGAAAATTCAAAAGATGAGTATCAAGTGTTCATAT10.37735849056603776No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.37735849056603776No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC10.37735849056603776No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAG10.37735849056603776Clontech Universal Primer Mix Long (100% over 22bp)
GGNNAGCAGTGGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAA10.37735849056603776Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT10.37735849056603776No Hit
GAGTACATGGAATCGGACGAGATTACATTTTCCTACACGTCGCTCTGTCC10.37735849056603776No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGA10.37735849056603776No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAATTTTATT10.37735849056603776No Hit
GTACATGGGATGATCGCCCCGAGCTACACTCTTTCCCTACACGACGCTCT10.37735849056603776Illumina Single End PCR Primer 1 (96% over 30bp)
ATACAAGGATGACTCATACACAGATGAAGTGGCCCAGCCTGCCTGGTTGG10.37735849056603776No Hit
GTACTTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA10.37735849056603776Clontech Universal Primer Mix Long (95% over 24bp)
GTGCTGGATTTACAGGCGTGATCCACTATGCCTGGCTAATTAAACCACAT10.37735849056603776No Hit
CCACAGACCTAAGCAATACTTTTCTAAAAACACACTGGGATTCATAGTTT10.37735849056603776No Hit
CCGCAATTTCGGCGAGCCGAGTTTCGATCGCAGTGCCGCCGATCAGAACC10.37735849056603776No Hit
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
CAGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CACCAAGACAAAAAAGCAGCAGAGCTGAAAGGATGTTGCTTTGGCTTGTT10.37735849056603776No Hit
CCCAAGAGGGGAGGTCTGCGTGTTGAGCCAGGGAGGAAGGAGCCTGCAGC10.37735849056603776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATACACTGCGTTGATACCACTGC10.37735849056603776Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.37735849056603776No Hit
GATTGCCAAGAAGGTCCTCGGCCGCGCGTTGCGACTCGTCAGCCATTCGA10.37735849056603776No Hit
GACAATCATATCATCTTGCAAACAAAATGAGTTTTATTTCTTCTTTCCCA10.37735849056603776No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAATTTTTATTTT10.37735849056603776No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT10.37735849056603776Clontech Universal Primer Mix Long (96% over 26bp)
GCACTCATTAACCCGTCATTTACATTAGGTATATCTCCTGTCTCTTATAC10.37735849056603776No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGCTTTTAAACCA10.37735849056603776No Hit
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGAAA10.37735849056603776Illumina Single End PCR Primer 1 (100% over 27bp)
GGATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC10.37735849056603776Illumina Single End PCR Primer 1 (97% over 49bp)
GGGCTCCCTTGGCCTTGGACCTAAAAGCCAATCCTCTGCTTGTGACTCAC10.37735849056603776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.37735849056603776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGTGCGTTTTAACCAATG10.37735849056603776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACA10.37735849056603776No Hit
GGTATCAACGCAGAGTACATGGGGGAAGCAGTGGTATCAACGCAGAGTAC10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGGTAT10.37735849056603776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGCACGGCGTTGATAACA10.37735849056603776No Hit
ACACAATCAGAACATTCATGATATCTCAATCACAATTTGATGGGAAAACC10.37735849056603776No Hit
GGGAGTCTCCCAGGGGAGAACCTGGGAAGGATGGACTCACTCTGCTGGAT10.37735849056603776No Hit
AATTATGATTATAAACATGTTTATATCAGTCTTGTATTTGAAAAAATTGT10.37735849056603776No Hit
ATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGC10.37735849056603776Clontech Universal Primer Mix Long (96% over 27bp)
CTCCCGACCTCAGGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGAT10.37735849056603776No Hit
AGCATGATTCTTACAGGGGAAGGATTTGAGGGTTCCTATACAGGATACAG10.37735849056603776No Hit
ATGTTGGCCAGTCTGGTCTTGAACTCCTGACCTCAAGTGATCCGCCTGCC10.37735849056603776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)