FastQCFastQC Report
Tue 10 May 2022
EGAF00003673751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348610
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5480.15719572014572158No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5180.14859011502825506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4910.14084507042253522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.14055821691861967No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4640.13310002581681535Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG4110.11789679010929119No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3800.10900433148790913No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3540.10154614038610482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)