FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA338.396946564885496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT205.089058524173027Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG92.2900763358778624Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG82.035623409669211Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT71.7811704834605597Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG61.5267175572519083Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC51.2722646310432568Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC51.2722646310432568Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51.2722646310432568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41.0178117048346056Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG41.0178117048346056Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC41.0178117048346056Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC41.0178117048346056Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30.7633587786259541Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.7633587786259541Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.7633587786259541Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTCTGAAGCCTGTTACCTGGAGGCCTCATCTGCATGATAAAACCTTG30.7633587786259541No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.7633587786259541Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.7633587786259541Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.7633587786259541Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACCCATGTACTCTGCGTTGATACCACT30.7633587786259541Clontech SMART CDS Primer II A (95% over 22bp)
GGAGATCATCGATACCGTTCTCAAGGACCCGAGCCTGCTGGCCAAACCGG30.7633587786259541No Hit
TTTTTAACTTTTACTTTAGGTTCAGAGATACATGTGCAGGTTTGGTATAT30.7633587786259541No Hit
CTCTTAGAGATGCCATTTCCAGAGCATAAGTGACAGGCCAGCATTTTCAA30.7633587786259541No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG20.5089058524173028No Hit
ATTTAGGGCTGGGCGTGGTGGCTCATGCCTGTAATCCCAGGACTCTGGGA20.5089058524173028No Hit
TAACATGTCTGAGGAGTTATTATGGCTGACTGTGGATTTAATCCATGCTT20.5089058524173028No Hit
GTCCCTAGGAGACCAACACTCCAGCTGAGCTGGCCTTAGCCCCAGCCCCT20.5089058524173028No Hit
AGATGTTTTGTGGCTGGCCTGCGCACCTTTTATGTCTAGTCAAAGTGAGT20.5089058524173028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATCTGTCTCTTATACA20.5089058524173028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC20.5089058524173028No Hit
ATATATAGTAATAAATACCTTACAGGTTCTTGTTAAAACGTTACTGCGTA20.5089058524173028No Hit
GTATGAGAGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGC20.5089058524173028No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.5089058524173028Clontech Universal Primer Mix Long (96% over 25bp)
ACCCCTTGCCAGGTGTGGTGGCTCACACCTGGCAAGGGGTAGCTTCTTAA20.5089058524173028No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTACCTGTCTCT20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.5089058524173028Clontech Universal Primer Mix Long (95% over 21bp)
GTGGAAATCCCTCCATAATACATGAAGTGAAAACAAGTAAGTCTGTATAT20.5089058524173028No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTGGGCTTGCTGCCCTAAACATTAATGTACAAGTTTTTGTGTGGACA20.5089058524173028No Hit
TTATGCATAAAGCCAAATTTCCAGTTTAAGTAATTGCCTACAATAAAAAG20.5089058524173028No Hit
GGTCTCTGCAGAAGAAGGTTGGGGTGGCTCAGGCTGCTGATCCAGCCAAG20.5089058524173028No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.5089058524173028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCATGATGAGCCTGCTGAAGGACGATGTGTACTGTGAGCTGGCGGAGA20.5089058524173028No Hit
GAAGGGGAGATAGTGGAACTTGCGGAAAAGGTGGTCTTCCCTGGGCAGGA20.5089058524173028No Hit
AGAAAGAATGGAGGAGGACTTAAGAGAAAGTAATCCACCCAAAAATGAAG20.5089058524173028No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.5089058524173028Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.5089058524173028No Hit
CTGTAGTCACAATCCCGGATGTCCGCAGAACAGCTGCAGTTCCGAATGGT20.5089058524173028No Hit
AGCCTGGGCGACAGAGTAAGACTGTCTCAAAAACAAACAAACAAACAAAC20.5089058524173028No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.5089058524173028Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTAAAGGGACAACAAAGGCCACAAAAGTAAGCATGAAAGGTGGAGGA20.5089058524173028No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGATGCGGCGGCAGCCGCCCTGCTGCCGTCGGCCCAGATCTCGGCG20.5089058524173028No Hit
GTTTCAAAGAACATCTTTATTTCTGCCTTCATTTCATTATTTACCCAGTA20.5089058524173028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.5089058524173028No Hit
CAATAAATGTATTCTGCCTTATCGCTGGATATTCATTATGGGATTTTCTC20.5089058524173028No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20.5089058524173028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA20.5089058524173028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTCCCACTTTCTTCTAAGTGCAATCCTTTCATGTTCACTTGCTGTTTT20.5089058524173028No Hit
ACATCTGTTAGTTCATGATTAAAAGCAAAACTGAAGGTGACCCTCTCAAT10.2544529262086514No Hit
ACTTTGTAGTGTATTTAGGTTACAAAAAATTTACAAAGTCTTCCTTGGAT10.2544529262086514No Hit
GTTCAGCTCTACACATTTTGAGACAGAGCGCAAGCAGGAAACACAGCAAG10.2544529262086514No Hit
CTCTTAAATTTAGTGTATGTAAACAGCTTACAAATACGTATTGTCTAAAT10.2544529262086514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAAA10.2544529262086514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATTTAATCTGCGATGAA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTCTCTTATA10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAAAAGAGACTAGTTGACATGTCTGCTAAAAAATTTAAGCAATCTCA10.2544529262086514No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG10.2544529262086514No Hit
GGGCATCATCAATTTCGAGCAGAAGGAAAGTAATGGACCAGTGAAGGTGT10.2544529262086514No Hit
GGTAAAGATTTAAAAATATTTTGGGGCTAGGCATGGTGGCTCATTGTAAT10.2544529262086514No Hit
AATCTGACCTTGGATAAGGGTCACGAACAGCAATGGTGTCTTTCAGGAGG10.2544529262086514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAATCAGTGGTA10.2544529262086514Clontech SMART CDS Primer II A (100% over 26bp)
TTCCATAGGTCTTCTTTAGTAATGATGTGGAAAAGCATGATATACTTGCA10.2544529262086514No Hit
GGGTTGAATTGGTCGAAAAGTCCTCAACGTCCTGACGGGACTCCCCTGAA10.2544529262086514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGTT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATC10.2544529262086514Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATAGTTTAGAGGGAAATTATGTGGTCAATCTTAAAATAAGTTTGATGTG10.2544529262086514No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACT10.2544529262086514Clontech SMART CDS Primer II A (96% over 25bp)
CTGCTGTGCTCTCGCATAATCGCTACCACGTGCCAGTTGTGGTTGTGCCT10.2544529262086514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTT10.2544529262086514No Hit
AACTTGAACCCAGTAGGCAGAGATTGTAATGAGCTGAGATTGTGCCACTG10.2544529262086514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT10.2544529262086514No Hit
ATTACTCAAAAAATTCTGCATTGTAAATAATACCAGGAATATTGCATTTA10.2544529262086514No Hit
CCTGTGGTCCCAGCAATTTGGGAGGCCGAGGCAGGTGGATCACCTGAGGT10.2544529262086514No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAACTCCTCCTTATAAAATCATCAGATCTCATGAGACTTATTCACTA10.2544529262086514No Hit
GTCTAGATTTTACCTGAAGACAATCCCGTTTCCCACGAAATCCTCAAAGC10.2544529262086514No Hit
TCATAGAATTGTATGCGTTGGGTGCTGTGGGGAGGTGTTAGAGCTGTCTC10.2544529262086514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGA10.2544529262086514No Hit
AAGCAGTGGTATCAACGCAGAGTCATGGGAAGCAGTGGTATCAACGCAGA10.2544529262086514Clontech Universal Primer Mix Long (95% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTTCCTGTCCG10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA10.2544529262086514Clontech Universal Primer Mix Long (95% over 21bp)
GGCCCAGGATGGGAGAGCCGCCTGCCCTGACATGGGAGGGAGGCTGGTGT10.2544529262086514No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA10.2544529262086514Clontech Universal Primer Mix Long (95% over 24bp)
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA10.2544529262086514No Hit
AAGGCGAAGAGCGTAAAAAAAATGGCTGCCTTGAGCAGCCACAGGAGGTA10.2544529262086514No Hit
CTATTTAATTTTCCTATTTATAAGCTATATTGCTATCTCCCTCTAGCCAG10.2544529262086514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGGCTTCCCATGTACTCTGCG10.2544529262086514Clontech Universal Primer Mix Long (95% over 22bp)
GATAAGGCCAGATTTTGCTCATTATTTTTCTTAGTAACTACCACTAGTTC10.2544529262086514No Hit
GTGTAAGTTCACTGGCCTCATGGAGGTCGACGTCAAGAGGCCGCAACTCT10.2544529262086514No Hit
GAATCTTGGTGTAGCCAGCCTCCATTTTTACAGATGCCACAAGTTGTGAT10.2544529262086514No Hit
GAGTACATGGGCAAGCAGTGGTATTAACGCAGAGTACTTGGGAAGCAGTG10.2544529262086514Clontech Universal Primer Mix Long (96% over 27bp)
TGTTTTTTTTTATTTGTTGCTTGCTGGGATTTCTCCCTGAAAAAACATAC10.2544529262086514No Hit
GCTTCCCATGTAATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.2544529262086514Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.2544529262086514No Hit
ACTCCCAGCTATCCCTGCAAAACAAACTCCAGAAAATTACCCTCTGCGAT10.2544529262086514No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.2544529262086514Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTG10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTAGAGATAGGGAAGGAGTAATTGAAAAGTATTAGAAAATAAAATGATCC10.2544529262086514No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGCAGCT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACTATGCGTTGAAACCA10.2544529262086514No Hit
GGGCCGAGACAACTGGCTCGAGGAGATCCAAAGCGCGCACCGGGGTGAGC10.2544529262086514No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGGAGTCTCTCAGAAAGTTCTATCCTACGGTCTTAC10.2544529262086514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.2544529262086514Clontech SMART CDS Primer II A (100% over 26bp)
GTGTGTGTATGTGTATGTGTGTGCATGTGCGTGTGTGTGTCTGCATATTT10.2544529262086514No Hit
ATGCAGAAAAGGCCTTTGACAAAATTCAACAACCTTCATGCTAAAAACTC10.2544529262086514No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACG10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTGGTTA10.2544529262086514No Hit
ACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2544529262086514Clontech Universal Primer Mix Long (96% over 27bp)
GCTTACGTGTCCCTAATCCCTCTGGAAGTCAGCAGTTATTAAGCTCTCTG10.2544529262086514No Hit
TCAGATGACTATGCTCAGCTGTGTAACATCCCCGTGACGGGACGCCGGCA10.2544529262086514No Hit
TTCCCAGACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAAGCTGGAGTGCAATGGCGCGATCTCGGCTCACTGCAACCTCCGCC10.2544529262086514No Hit
GTGACATTTCAAAAGTGTCATGGATGCTCACCAAACCTCAATTCCTCTCC10.2544529262086514No Hit
GCGCAGGCCGCGGACCCGACCGCGGCAGGCGCTGTAGCGGTCGCCGCCGT10.2544529262086514No Hit
GCTTGTCGCTACCCGGGCCGCCAGGGCCGCCCTGGCCACCGGCCGCCGTC10.2544529262086514No Hit
ATCTGAAATGGATTCTATGCCTTATACACAGAACTTTATTTCCTGTAATC10.2544529262086514No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
AGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTA10.2544529262086514No Hit
GTGGTATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAA10.2544529262086514No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTG10.2544529262086514Clontech Universal Primer Mix Long (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.2544529262086514No Hit
GACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAAC10.2544529262086514No Hit
GCTTTACGGAGAAGTTTAAAAGTAGAAGGCAAAGAATTAAACATACTGAC10.2544529262086514No Hit
GTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGCATAAGCATTGCGGATCTCCATGACCATCAGCCGGATGTCCCGG10.2544529262086514No Hit
TGACTGAGGCATGCCCTTTGTGGGCCAACTGAGTATGTTTTAAGTAGGGA10.2544529262086514No Hit
TTTCAGTAACATGCCATTTTGATTTTTAGTGGATTATCTTGATGGATCTC10.2544529262086514No Hit
GGAGATGTATCTACGTTAACTTTCCCCTATGGGGTGATAGTTAACTGTTG10.2544529262086514No Hit
GGAACCTCCAAACTGTTCTCCATAGTGGCTGTACTAACTCACATTCCCAC10.2544529262086514No Hit
CTATTCAGGATTATGAAACTGCTCAGGAACACAATGAAAATGGTCAGCAG10.2544529262086514No Hit
CTCCTTATCAGATAAATGGTTTGCAAATATTTTCTTCCATTCTATAGGTG10.2544529262086514No Hit
CTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCAT10.2544529262086514No Hit
GGTATCAACGCAGAGTACATGGGAAGCGCAGTGGTATCAACGCAGAGTAC10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATAGAAATGAATTTGGAATGCATGGAAAAGCTGCTCACCCTCTTTTTGTT10.2544529262086514No Hit
CCTTCCTTCCTTCCTTCCTTCCTTCCTTCCATCCTACATTCTTTCTTAAT10.2544529262086514No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACTCTGCG10.2544529262086514Clontech Universal Primer Mix Long (95% over 22bp)
CAGCAAGATGGCGGCGGTCTCAATGTCAGTGGTACTGAGGCAGACGTTGT10.2544529262086514No Hit
CGCCATTGACGGTGTAGGTGACGGGCGGCGCGACCACGCCGGTCTGCGCG10.2544529262086514No Hit
CTCGTGATCCGTCTGCCTCAGCCTCCCAAAGTGCTGGAATTACAGGCGTC10.2544529262086514No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC10.2544529262086514No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TCTAAAAGGGTGTTATGGGAATTAAATGAGCAAGATACATGAAGGTGTTC10.2544529262086514No Hit
ACAATGGCCAAATATACGATGATGATCCCGTAAGATTACAATGGAGCTGA10.2544529262086514No Hit
GTGCAGGGAGCTACTCATTCTCTCCAGGAAAAGTGAGGAAGGCTTCATAA10.2544529262086514No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA10.2544529262086514No Hit
CAATACGTCCCGGGCGCCACTCAGTCTGAAGCTGGCCCAAGTTTATCTCT10.2544529262086514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACTGTCTCTTATACACA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.2544529262086514No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTC10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTTCATAACGAGTTGGAGCCTAGAGTTGATCGACTCCAGCGACTTTTT10.2544529262086514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAAAG10.2544529262086514No Hit
TGGAGTGTCTCTCTGTCACCCAGGCTGGAGAGCAGTGGCGCGATCTCGGC10.2544529262086514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTTATAC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
AGTCTGAGGGGTGCTCAGGGTGCCCCCGCGCGAGATGCAGTCCTCGCTGG10.2544529262086514No Hit
CCCCATCAAAAAGTGGGTGAAGGATATGAACAGACACTTCTCAAAAGAAG10.2544529262086514No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
AGGCAATACAGTGAGACCTTGTCTCTGCAAAAAGTTAAAAAGAAGAAAAA10.2544529262086514No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.2544529262086514Clontech Universal Primer Mix Long (96% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGAT10.2544529262086514Clontech SMART CDS Primer II A (96% over 25bp)
ACATGGAGAAACCCTGTCTCTACTAAAAATACAAAATTAGGTGGGTGTGG10.2544529262086514No Hit
ATCTCAGCTCACTGCAACCTCCACCTCCCGGGTTCAAGCAATTCTCCCTG10.2544529262086514No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTCC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTAACAACGCATATTACATGGAAGAATTGGTA10.2544529262086514No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TGCCACCACACCCGGCTAATTTTTTTGTATTTTTAGTAGACACGGGGTTT10.2544529262086514No Hit
CTTTACTCCTCAGTGTGGAAAATGCAGAGTTTGTAAAAACTCTGCATTTT10.2544529262086514No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.2544529262086514No Hit
GGCGCTTTTCCATCATCAAACCAACCGGCTCTGCTTAACCGCGGCTTCGA10.2544529262086514No Hit
TGCACACACACACAATCACGCATACCTGTAGGCATGTGTGTAAACACCCA10.2544529262086514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGGGTAT10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCTACACAAGCTCTCTTGCCTGCTGCCATGAAAGACATGCCTTTGGCG10.2544529262086514No Hit
GGCCAGAAACAAAGGAAAGCAATACTCTTGAATAGAAAAATGATTAATTT10.2544529262086514No Hit
TCTAGGAGGACCCTAGCAACCCTGAGGACCTCAGCCCTCCGACTGCAAGC10.2544529262086514No Hit
GTACATGGGAAGCAGTGGTACCAAAAAAAAAAAAAAAAAAAAAAAGTACT10.2544529262086514No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTA10.2544529262086514Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC10.2544529262086514No Hit
AAAACTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCCAGGAAGCAGAAGGCCATGGCTGCTGGACACTGGCAGGAGCAGAT10.2544529262086514No Hit
ATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATAAGCGGGCGCTACGCCACCTCGAGTCGTGGTGCGTCCAGAGACA10.2544529262086514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.2544529262086514No Hit
ATCAAGCAGAGTACTTGGGAAGCAGTGGTATCAAGCAGAGTACTTGGGAA10.2544529262086514No Hit
TACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT10.2544529262086514Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGTTGGACGCGGCGAGCAGGTCCAACTCGCGCTCGATGCGCCCGGCCTC10.2544529262086514No Hit
GATACCACTGCTTCCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT10.2544529262086514No Hit
GAGTACATGGGTGTTTTGCAAACATATGTGGAGCAAACATTACGGATATA10.2544529262086514No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CTTCAGGAAGGCACAGGGCCCCACACCCCCGAGATCCAAGCTGCACTGGC10.2544529262086514No Hit
GTACAGGGGAATCAGTGGTATCAACGCAGATTACATGGGAAGCAGTGGTA10.2544529262086514Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAA10.2544529262086514Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATGC10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTCCCATGTACTCTGCGTTGATACCAGTGCATGAAAAACACAATTCG10.2544529262086514Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTGTAGAATAGGAGCTCAATGAATGTTTGTTGAATGACTAAAAGCCGCAT10.2544529262086514No Hit
CTTACGATCCGCACTGGCACGACAGCTATCTGATTGGCGTCACGGAGCAA10.2544529262086514No Hit
GAAGAAGACAGTGGAATATGTCCTAATGGTGCCCTCAAACTCCACCATGG10.2544529262086514No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTAACCACTCATACAGAGACAGCTACACTGATTTTGGGGCCAGGGTAT10.2544529262086514No Hit
ATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATAC10.2544529262086514No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGACTCTGCGGTG10.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGCAGTGGTATCAACCCAGAGTACATGGGAAGCAGTGGTAA10.2544529262086514Clontech Universal Primer Mix Long (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)