FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCAAGCAAACAGACAAACCTCAGTCAGTGGAATGAGAAGCAGGAAT45.405405405405405No Hit
GCCCCTGCTCCGCAGGAGGCCACAGCCACATTCAACAGCACGGCTGACAG34.054054054054054No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA34.054054054054054No Hit
AGTGCAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGC34.054054054054054No Hit
GTCTTCACCAACGCCGCCAAGACCCGCACGCGCGGCGTCGAGATCACCGG34.054054054054054No Hit
CACCGCCATCACCAGCACCGCCCCCATGATGCCGAGCGGCGCGACCAATC34.054054054054054No Hit
AATATATCGCTACCAATATCAAATCCAATATCCGTGAGCTGGAAGGTGCC22.7027027027027026No Hit
CCGGTTCCAGCAGCCGCGCGTAGATTTCTTCCCAATGGGCCAACGACGTG22.7027027027027026No Hit
CTGGGATACCGCACTCGCGTCGCAGTTCGCCGGCCCACCGGTGTGGGTAC22.7027027027027026No Hit
GCAAGCTCCACCTCCCAGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGA22.7027027027027026No Hit
CGTGGGGCACTACTGCTGTCACTGCTCTGAGGGAGAAAGACGACCAGCAG22.7027027027027026No Hit
GCTCTGTGATCTCTACCAGCATTGCAAACACAGTTTCCTGCAGGGAGAAA22.7027027027027026No Hit
GTGCGATTCGATGGGGCCATGGGCCGGAGCCTAGGAGAAGCCGCGAGCTT22.7027027027027026No Hit
ACGGCTCCGGCATCACCGCGGTGGTGCTGGTCGTCGCAGTGACCGCGGTC22.7027027027027026No Hit
AGTGTGCACAAATGCATGGGGGCATGGTAGAACATGGCATTGCGTGTTTG11.3513513513513513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.3513513513513513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGAGAGGAGGCTGCAACGCCGAGCGGAGGAGGCAGGAACCGG11.3513513513513513No Hit
TTTTATAAGTTAATCAAGTGCATACCACAATAGATTTTTGGTTGTTGTTT11.3513513513513513No Hit
GAATGGTTGAACTCTATGGGATGAATGCACACATCACAAAGAAGTTTCTC11.3513513513513513No Hit
GGTGTCTTCGCGGAGTCCACGATCAGGTCCAAGTAGCGCCCCAGGGTAGG11.3513513513513513No Hit
GTACCGGAGAAGATCAGGTCACCTGGGTAGAGCTCGCAAATCGCCGAAAT11.3513513513513513No Hit
CCATTAGATGATTCCATTCGTGACAATTCGATGATTCTCTTCGATTCCAT11.3513513513513513No Hit
ACCAAAGAGATCCCAGACCAGATGGATTCTAATTCTAGCAGATACAAGAA11.3513513513513513No Hit
AGTCAGTTTGGTTGTTACGATATGCTGGTGAGGTCTTTCAGTATCTTATC11.3513513513513513No Hit
GGGCGGGCGAGGCGTCGTCGGTGATGTTGGTCACTTCCGTCTGCGCGACA11.3513513513513513No Hit
TTGTACCGGCCGGTTGCCGCGATGCGGCGGCGCGCTTACCTAGCGGGTCC11.3513513513513513No Hit
AGACAGGCAGCAGATCATGGAGCTGTACCTCAACTCGATCGAGCTGGGGC11.3513513513513513No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT11.3513513513513513No Hit
TCACTCAAAGCTTCATGGTGCATTTCGACAGAATTTACTTCCGATGTAAC11.3513513513513513No Hit
GAAGCGTCAGATGTGTATAAGAGACAGGAGAGAAGCAATCGGAGAAACTT11.3513513513513513No Hit
GTTCCCTACCTTACCCAAGGTCACACAGTTAATCGCTGGCAGTGCAGGGA11.3513513513513513No Hit
GAATGCACACGATTTAGCACAAGTGGAACACCAAGTAGGGAAAGTCAGGA11.3513513513513513No Hit
CCCACCGGCAGTTCCGCCACCCCCCGGTCGGCATAGTCGGACAGCCGCAG11.3513513513513513No Hit
GTCCGATTCCCTCCATGTGTTCTGGGATTGGAAATGAACTAATTAGAGCT11.3513513513513513No Hit
CACCAAGACTTGCCAAGTCCTGGAGGCCCTCAATGTGCTGGTGAATAGAC11.3513513513513513No Hit
AGTGAGTACTGTTCTAAAGCACACGGGTATAACAGGGAGAATTAGTGCTC11.3513513513513513No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.3513513513513513Clontech Universal Primer Mix Long (96% over 26bp)
AGTATGACCAGTGTCGCATACAGGTCAGGCTGCCAGATGGGACCTCACTG11.3513513513513513No Hit
AATATGGGAAGGCCGAGGAAGAGGCCCTGTCCCGGGATAGGTTCAGGGAG11.3513513513513513No Hit
GTCTCTGACCATGAGGCCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCC11.3513513513513513No Hit
ACGTCGAACAATGTGGGGAGCAGATAGGGCATGTGCGCCTGGGCCGCGGT11.3513513513513513No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA11.3513513513513513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTATTCGTTTAAACCAAAGCTTC11.3513513513513513No Hit
AAATATGGAAAGGAAAAACTGGTACCAATCACTGCAAAAACACACCAAAA11.3513513513513513No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT11.3513513513513513No Hit
GCGGGAAATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTCGCCCTGG11.3513513513513513No Hit
ATATGTCATCACACATTTAAAAATGTGACTCCTTCTGCAGCAGCAATGGG11.3513513513513513No Hit
GGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATTA11.3513513513513513No Hit
GTCCTGTTAGAGGCAATGGCGCTGGGCGCAGCGGTTGTCGCGACCGACTG11.3513513513513513No Hit
TCTACTGGTCTTGTCAGGTGCCTGGGGCATGCAAATGTGGGTGACCTTCG11.3513513513513513No Hit
GTATCTTATGGAAGTAACACATGACCTTAGACTACGACTCAAGAACTATA11.3513513513513513No Hit
ACCCATGGCATGCCTTGATGTGTACAACAAGCTGCTGGCATACAGTTCCA11.3513513513513513No Hit
TCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCGTCGCATCCTGGGGCT11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)