FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACCCTGGCTGCCTAGGTGTGTGGCCCCCAGAACCTTGGCGCTCGCAC45.405405405405405No Hit
ACCCTGAGGAGTCACACTGAGTTCCAGTGACCACCGTGGTGGTGGCCATA34.054054054054054No Hit
GCTACTACGTGTGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGC34.054054054054054No Hit
GGCCTTGGTGCCGTTGTTGCCGGTGCCGTCGGTGCTGACAATCTCTGACG34.054054054054054No Hit
ATAAAAGGAAATGCAGCAAATGGCTCAGTATCGTATTGAAAAAAAATCCA34.054054054054054No Hit
AAGGGGCGATCTCCGCGCCGACGAACCAGTACCGGCTGGAGAGCCTGTAA34.054054054054054No Hit
GCCGGGGACCGCGGGTGCGCCGCGCGGGTACATCCGGAGGCGACGCTCCA22.7027027027027026No Hit
GTGCCAGCAGCAGGCGAACGAGGTGACGGAAATTATGCGTAACAACTTCG22.7027027027027026No Hit
AGCAGAACCTGCCCGGCCGGCTGGTAGACCTGCCCCCAGAACAGGACGAT22.7027027027027026No Hit
TCGTCGTTCTGCAATTCGTTTTCGATCTTTTCAATACCGTGCATAACGGT22.7027027027027026No Hit
GTCAGGAGATGGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC22.7027027027027026No Hit
CCACAGGGCCCAGCCGCGACCGCGCGCCCACGTGTCGTCGTCGAGCGCAA22.7027027027027026No Hit
GTCCCGGTCGTCGCCACGGACGTCGGAGGGATCGGCGAGCTGGTCCGGGA22.7027027027027026No Hit
GGGCCGCCTCATCACTGGAGCCACCAGCCCAACCGTGTTGTATGTGAGTG22.7027027027027026No Hit
GACCAGGATGGTGCCGGTGCTCGGCGTCACGATGCCGCAGATGATCGAGA11.3513513513513513No Hit
GAGTGGTACCAAACTGCTCAATCAAAAGAATGGTTCCACTCTGTGAGAAG11.3513513513513513No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT11.3513513513513513No Hit
CTTGATCATAGTGAATTAACTTTTTGATATGCTGTTGGATTTGGTTTACT11.3513513513513513No Hit
GTTGGCGAGCATCTGTCCGAGCACCCAGTTGCGGCGATCGAGCGCGCGCT11.3513513513513513No Hit
GCTCGGCTTTGTCGGCAATCGGCCGCTCGAAGACTGATACTGGCGGAACG11.3513513513513513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA11.3513513513513513Clontech Universal Primer Mix Long (95% over 21bp)
TCCATATTCGAACCGGTTCTCTTTTTCTCTTTCATGCTACTCTTTAATTT11.3513513513513513No Hit
TGCAGAGGCCGCTCCATGTCAGCTTCTGAGAAGGCCCGTCTGGGGAAGCC11.3513513513513513No Hit
CACACAAACTAATGACTAACAACCTAAGAAGGGAAAAATGAATGGGGAAT11.3513513513513513No Hit
GTGCCAGGCTTGGGGGAACCCATTGCCCGAGCTCAAGTGTCTAAAGGATG11.3513513513513513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.3513513513513513Clontech Universal Primer Mix Long (96% over 26bp)
ATCTCACAGAGTTGAAACATTCTTTTGATTGAGCAGTTTGTAAACAGTAT11.3513513513513513No Hit
GCGTTGCGGCGCTCCTCGCGGTCGATGGTGAGCCACATGACGGGCCCGCG11.3513513513513513No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG11.3513513513513513No Hit
CCCAAGAACGTTTCCCACTGTTCGGCGATGATGTTCCGGTCGTACCGTGC11.3513513513513513No Hit
CAGCCTGAGAGCAGCTCCCTCCTTTTCCACCTGAGCTCTTCCTCCTACAC11.3513513513513513No Hit
CTCTGGTCCTCTCGTACTAGGAGCAGATCCTCTCAAATTTCCTACGCCCG11.3513513513513513No Hit
GGTTTGCAGGGCCCACATGGCAGCTGTGGTGCGGGGTTCCAGCCAGCGGG11.3513513513513513No Hit
GTCTATGCTGTCTCATAAGTCAAGTAGGTGGATGGGATAGAGATTCAAAT11.3513513513513513No Hit
GCCATCAGGTTACTAACATTGGTCCAGGGTTGTCACTAAATATAAATTCA11.3513513513513513No Hit
GGCTAACCAGGCACTTAACAACTGAGCCCCGACTTCATTCCCCAAGTCAA11.3513513513513513No Hit
TTGTTGGTTTTAGTCTGTTTTGTCAGAGACTAGGCTTGCAACTCCTGCTT11.3513513513513513No Hit
GTTATCGCCTTGCTTGATTTCAATGTTGGTTGAGAAGTGGCCATTGGATG11.3513513513513513No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT11.3513513513513513No Hit
GTATAACTTCTCTCATTTTAGATGGAAAGGCCAACGCACACAGCTTGGTA11.3513513513513513No Hit
ATCAACGCAGAGTACATGGGCAGTGAAGAATTTTACTTTGAGATTCCAAG11.3513513513513513No Hit
GAACGTGGTCACACCATTGAAATCTCCTTGTGGAAATTAGAGACCATCAA11.3513513513513513No Hit
CTGTCACCAAGGCTGGAGTGCAATGGTGCGATCTCAGCTCACTGCAACCT11.3513513513513513No Hit
GTATCTGGATACGGGTGATCAGATAGTCGAGCATCTGAAGACGTACTATG11.3513513513513513No Hit
CCATGTTAGATGGCCTGGGCCGCATCCGAGACCAACTCTCTGCCGTGGCC11.3513513513513513No Hit
GTTATTTATCCCACCATGAAAGATTTCAACCACTCATACCATGCCTGTGG11.3513513513513513No Hit
CCTTGAACGGAATTGAATGGAATCGTCATCGAATGAATTGAATGCAATCA11.3513513513513513No Hit
ACGGTGGCGGGGGCGCTGGCGCTCGGCAGCTTCTTTCGCACGCGCGAGCG11.3513513513513513No Hit
GTTGCCTACGGCCTCGGTGGACTGGGAAGTCGAAATCGTCGTCGTGATCG11.3513513513513513No Hit
GTTGAAGGTGGTCTCGTGGATGCCGCAAGATTCCATACCCAGGAAGGAAG11.3513513513513513No Hit
CCCATGGAAAGCTGCTGGGATAGAGTATATGAATGGCCCCAATCCTTCAT11.3513513513513513No Hit
CGCGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAA11.3513513513513513No Hit
TCCTTGAGTTTATCAGTAAGAATCTTGATTTCTTCCTCATATTTATCTTC11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)