FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCGGTCTCATGCAGTT42.73972602739726No Hit
CATGGTACCTCAGTGCATGCATGGAGCCATATCACAATTATTAATCTCAT32.054794520547945No Hit
TTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGA32.054794520547945No Hit
CTCCTTCCCTGTGGCTTCCTATTGGATATAGCCAAAGGAGAACAGTGCTC21.36986301369863No Hit
GGAAGAAGCTCCAGGGGCCCCGCGCCGCCCGCCTTGTGATTGCTGTTGAA21.36986301369863No Hit
GGGATGTTTCAATTGAAGTCACAGTGTTGAACATTCCCTTTCACAGAGCA21.36986301369863No Hit
GGTCCAACCCCCACGGAGTCTCGCTGATTGCTAGCACAGCAGTCTGAGAT21.36986301369863No Hit
ATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21.36986301369863No Hit
GTTTGCATTCAAGTCACAGAATTGAACACTCCCTTTCACAGAGCAGGTTT21.36986301369863No Hit
AATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAA21.36986301369863No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGT21.36986301369863No Hit
GAAAATTGAGAGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGG21.36986301369863No Hit
TCTCCAAGGGCTTCCACCACGAGTACTGCCCCTTCACCCAGACTGTGGAC21.36986301369863No Hit
GAAAATAACCTTTGCCCCTCATATCTCTGTTTTAATATGCTTACCCACAC21.36986301369863No Hit
CTCTTTTTTCCTCCCTATGCCAAACGCTTGCTTCCTCTTTTTCTGCCCTT21.36986301369863No Hit
GCCATTGGTAGGTTTCAAACTTTGCTAACAGCTCGCTAATCACTGGGGTT21.36986301369863No Hit
TTGCAGTACACTTTTCGTTTCCGCCTACCGCCGGCTCCTTTTTGCTTGCC21.36986301369863No Hit
CCACAGGCAATGCTCCAGGCCTGTGTTGGCTCCTGGCTGGACAGGGAAAA21.36986301369863No Hit
GAATAGAATTGAGGCCAGGAGGTGAAAGATGCAGGGAGAGAAATTTCTTT21.36986301369863No Hit
ACCTCCTAGTGCAGCCAATATTGGAAAAACCACTGTAGACCGAATGTGCG10.684931506849315No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC10.684931506849315No Hit
GCCAGTTTTTTATCAAAATTGGCATTGGCTTTTTCTTCGGCACTCAGTTG10.684931506849315No Hit
AGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAG10.684931506849315No Hit
CTTCAACAGACTGTATCCTCACCCCTATCTGACCTTATCGTTGCTCACAC10.684931506849315No Hit
CCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC10.684931506849315No Hit
GTTTCTGCTAAGTTACCTTTTGGAAATAAAAGTCAAGCAATACCCAGCCT10.684931506849315No Hit
GCACGAAAGGTGTAATGATTTGGGCACTGTCTCAACGAGAGACTCGGTGA10.684931506849315No Hit
CCTATCAGGGCGTGATCCCATTTCGTGAACTTATAGCCGATCTCGGCTGC10.684931506849315No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG10.684931506849315No Hit
AGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCT10.684931506849315No Hit
GAAGTAGACAAGAGGGTGAAACACAGAAGCTGATGTGACCATGGAAGCAG10.684931506849315No Hit
CTGCTGGGGTGGATCGGTGGGCCAACAAGGGAGGACTCATTGCATACAGA10.684931506849315No Hit
GTGAAGGGCAGTCCGGCCATTACAGGGCTCCTGAGCATTGACATCAGCAC10.684931506849315No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG10.684931506849315No Hit
CGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAG10.684931506849315No Hit
TATTGGTACTGTTCCTGTTGGCCGAGTGGAGACTGGTGTTCTCAAACCCG10.684931506849315No Hit
CTGTGGAGCTGTGTGGTCACCTCCCATGAGATTGCAACTTGTGTGTTGAA10.684931506849315No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10.684931506849315No Hit
GTGTTCTCGTTTGTTCTGTCGCCATTCAGCGAGCTGATCGGCGACCTTGC10.684931506849315No Hit
GAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAG10.684931506849315No Hit
CTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAGTACT10.684931506849315No Hit
CTGTTGAGTGAGTAGAACCTGGTTGGCTACGGGAGGTGGTGGATTCTTCA10.684931506849315No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTGTC10.684931506849315No Hit
AATTAGAAGTCTATTTATAAAAATGAGCCTCTTCCATCCCTTCCGCCAAT10.684931506849315No Hit
GACCAGCAGGAGACAGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCT10.684931506849315No Hit
CCCTTCTTCTTCCTGCGGGTGGACCCGGCCGGCAACGTCTCCAAGCGGTT10.684931506849315No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG10.684931506849315No Hit
ATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGA10.684931506849315No Hit
CTGTAGTGATGGACACCAGTTTTAGCCAACATAGCATAGTACTCTATTTC10.684931506849315No Hit
GGCCATCAGGTTCCAGCCGCCGTTGGCCACCGGTGCACCCAGTTCCAGGC10.684931506849315No Hit
CTCCAATTGCAGGACTGGTGACTAAACTTGCAGCAAATGTTGCTGAAACC10.684931506849315No Hit
TCCAAATACTCTAGTTGATATTGCTGTTTAACACGTTTTTAATCGTGCTA10.684931506849315No Hit
GAGGTGAGGTCCTGGGTTTTTGTGTGTTTCTCCTTCCTCCTGTATCTCCT10.684931506849315No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG10.684931506849315No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10.684931506849315No Hit
TCCTTATTCTGAGAATTTGGCCCCAGCTGTTTGCCTTTGACTCCCTGACC10.684931506849315No Hit
GCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACC10.684931506849315No Hit
TTGTTAAGCCCTTTTGGGGCAGCAAAAACAGCCCTTAATACCCATCAACA10.684931506849315No Hit
GTCACATAAGTGCTAAATGGGGGACTTAGGGCTGTTTGACCAGATCCTGA10.684931506849315No Hit
AGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTG10.684931506849315No Hit
GCCATTTTTACCTTTAACCTCAATTACCCAACCAGTAAAGGCATAACCTT10.684931506849315No Hit
GCCAAAGATGCCAAGTCCACGGACTGTCCCGTAAAGTATGCATAAACGTA10.684931506849315No Hit
GTGTAGATGATGGCAGGTTGGCCTCAGCAGACAGGCATACAGAGGTTCCT10.684931506849315No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA10.684931506849315No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA10.684931506849315No Hit
CGCTGGTCTCGTTCGATGATCCCATCGCGAACTGGTCGAGGTTCAGCTTG10.684931506849315No Hit
ACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTC10.684931506849315No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAAGCTGGCGGATCACTTGAAGC10.684931506849315No Hit
GGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC10.684931506849315No Hit
GTTCTCCAGCAGGCCTCTGCTGCAGCTTTTTCTCTCAGTGTTCTGGCCCT10.684931506849315No Hit
TGCCTAAGCTAAGAGATGCCCAATTGGCAAAAATATCACAAACTAATTTA10.684931506849315No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG10.684931506849315No Hit
GCATTATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTC10.684931506849315No Hit
GTGGTGGATTGTGCATGATGAGGCTAATCGACCAGCTCACCTGGTAGGTG10.684931506849315No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGG10.684931506849315No Hit
GTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTG10.684931506849315No Hit
GTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTC10.684931506849315No Hit
TTGTAAAAGATGTTTAGAGAAATATGCATGCTGGTATTTCATAAGCCAAT10.684931506849315No Hit
TTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCG10.684931506849315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTATCAACGCAG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GGCAAAGATGGCTTCCATATCCGGGTGCGGCTCCACCCCTTCCACGTCAT10.684931506849315No Hit
GTTCGCCCTCGGCGGGCCCGAACTTCTGGTGCAGACCGTGGAGCAAAACA10.684931506849315No Hit
TCCCATGTACTATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TAGAAAGTAAGCTCCAAGAGGGTTCCTGTGTCTGTTTCCCCTGCTATGTT10.684931506849315No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC10.684931506849315No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCTCC10.684931506849315No Hit
TCACTACGTCAAAGCTCATTGAACTTAAGATCTGTATATTTATTTCAGTG10.684931506849315No Hit
CCTACTAAATGGCCGTAGCACCCTTATTGAAAATGAATCGACAGTGTCAA10.684931506849315No Hit
ATGCTAGGGGCTTGCTGTTTTGGCCTATGTTGTGCTGAAACCCTGTTAGT10.684931506849315No Hit
CTACAAGGCAGTGTTTTGTAATTTGGTTTCATTATGAAGATCCAGTACTT10.684931506849315No Hit
TTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCC10.684931506849315No Hit
ATTCCATTCGGTTCCGTTTGATGATGATTCCGTTCGATTCCATTTGATGA10.684931506849315No Hit
ATGCAAGAGACTGATGTCTCCCTAACTGCTTTGCTCTACTGTATAAAGAA10.684931506849315No Hit
TTCCTGAGCTGCACCCTGTAAGCTCTCTCTAGGCCGTGAAGCAATTTTAG10.684931506849315No Hit
GTGCCCGGGAATCCCAGCCCTTATGGCCCTCTTCATTTAGCGTCTTGCTT10.684931506849315No Hit
ATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCA10.684931506849315No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA10.684931506849315No Hit
GCATGAACCACCGCACCTGGCCCAGGCAGTTTACTTCTAAAATCTTTGCT10.684931506849315No Hit
GGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG10.684931506849315No Hit
GCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTAT10.684931506849315No Hit
ATACAAACTACCATCAGAGAATACTACAAACACCTCTACGCAAATAAACT10.684931506849315No Hit
TATCATCAAATATGTCCATGCAACCATTTAAAATGATGATTTCGGACATG10.684931506849315No Hit
GATAACTGGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCT10.684931506849315No Hit
GTCAATCACCGCGACTTCTACATCTGCCACTTCCTGCTGCACACCGACCG10.684931506849315No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT10.684931506849315No Hit
GTGTTGAAGGTCTCAAACATGATCTGGGTCATCTTCTCGCGGTTGGCCTT10.684931506849315No Hit
CCTTAGGTCAGTTTTGCTGGCTTATAGACTTTATCAGATCCTGATAGAGC10.684931506849315No Hit
GCACCGTCCCCTGCAGGGCGGCGATGTCGGCGCCCTGTTCTTCGGCGACG10.684931506849315No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10.684931506849315No Hit
ACCCCATCTCTACTAAAAACACAAAAATTAGCTAGGCATGGTGGCGTGGC10.684931506849315No Hit
CTTCTGAGCTTTCTGGGCAGACTTGGTGACCTTGCCAGCTCCAGCAGCCT10.684931506849315No Hit
TCACGGCCGCGCGGTCCGAAATGGCCATCACCTCGTCGAGCCGGTGCGAC10.684931506849315No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAACTGT10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
CCTCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG10.684931506849315No Hit
CTTATAATCCTCCCTGAGGAGAGGAAAAAAATATTTTTCTTTTGGAACTC10.684931506849315No Hit
GCTTACGCCAACGCGATCAAGGGTGCCAAGTCAGTGTTCTGGAACGGACC10.684931506849315No Hit
GGTCCCCAACGCCAAGAAGCTCAGGCGGAAGGAGCAGCTATGGGAGAAGC10.684931506849315No Hit
GCGCGTGCCCGCCGGCGGGAACAGGCGGGGGACCGGCTATCCGAGGCCAA10.684931506849315No Hit
GGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGG10.684931506849315No Hit
GGCAGATACTTTATCAATGGAGGGAGCAGCATACGAGGTTGGTGCAATCA10.684931506849315No Hit
GTGCAGATTAGGCGGGTGGAGGAGCGGCTCTCAGCCTTGGGCAATGTCAC10.684931506849315No Hit
TAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACAT10.684931506849315No Hit
GGATGGATGGGTGGATGATGGATGGATGGATGGATGGATGGATGGATGGA10.684931506849315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)