FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA153.4482758620689653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132.9885057471264367Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG112.528735632183908Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC81.839080459770115Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC81.839080459770115Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT71.6091954022988506Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA71.6091954022988506Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC61.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC51.1494252873563218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC51.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG40.9195402298850575No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
CCCGCGCTCTTCCGCCAGCCAGCGGGCGAGCCGCGCCACGCCGGTCTGCC40.9195402298850575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACT40.9195402298850575Clontech Universal Primer Mix Long (95% over 21bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCTTCAATCAACAGAAAAGCTAATTGTCCACCATCCAAAGCGGGTAAAGG30.6896551724137931No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.6896551724137931Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GATGCGCATCAACGTCGCGACCTCGCAGGACGCGGCCTTCTGGCGCGCGC30.6896551724137931No Hit
CAGACTACATGGGGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC30.6896551724137931Illumina Single End PCR Primer 1 (100% over 37bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGCTGTC30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGAAGCAGTG20.45977011494252873No Hit
CCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAGA20.45977011494252873Illumina Single End PCR Primer 1 (100% over 24bp)
GCTTCCCCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACAT20.45977011494252873Clontech SMART CDS Primer II A (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGATACCACTGCTTCCCATGTACT20.45977011494252873No Hit
CGCTTCGGCGACGCCCTCGCGCTGGATCTTCTCGATCGAGCGCAGCACGC20.45977011494252873No Hit
AGCCACTGGTGTGTAAGTCCTGGAGCCCAAAGGCCGGAGCCTGGAGTTCT20.45977011494252873No Hit
GTCCTGTGACCCGATCGGAATGCACTCCTCACCAGGGTGGGCGTTTCAGC20.45977011494252873No Hit
CTCTTCCGATCTCATGTTTACTGGGAGCTGCCGATACCCTGTCTCTTATA20.45977011494252873No Hit
TCTGCGGGCCGGTCTCCATGGCCTCGTAGATGTGCTTCTCCTCCACGCCC20.45977011494252873No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCATTTCTTAAAACTAATTGAGTTCCACCTTTTCTGTTTTTAGCAGTCAT20.45977011494252873No Hit
GCTTCCCATGTACTCTGCGTTGATACAGTGGTATCAACGCAGAGTACATG20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGAGTTGAT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTTGTTATGGCTCGGCCACACATAAATCCCGTTTGCCCGCTCGGACA20.45977011494252873No Hit
GCTGAAAGACTTTATCTGGTGGGAACGCGTACGTGAGCTTATCGGAGAAG20.45977011494252873No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTACTGCGTGCCGCCGGTCAACCTCAAGCAGCACGTCGGCCAGCGCG20.45977011494252873No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGA20.45977011494252873Illumina Single End PCR Primer 1 (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20.45977011494252873Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTGGAGGACGTGCCCGGCGTGACGCGCGACCGCGTCTCCTACGACGC20.45977011494252873No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTCTGTCTCTTATACACA20.45977011494252873No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GGATCAGACATGCTCATCTTCGGACTCCCTTACCAGCTCGCCTTGGTCAT20.45977011494252873No Hit
CAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGACCACCGAGATCTA10.22988505747126436Illumina Single End PCR Primer 1 (95% over 22bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCGAGTTCATGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACT10.22988505747126436Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAGTACCGTT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
CAGCCAGACCTCTTCTTTCAGCAGGAAGCTGTCGCCAGCCTTCGGCGTCA10.22988505747126436No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.22988505747126436Illumina Single End PCR Primer 1 (100% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGCGGCCCCC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCACGGCCGACGGCAAGACAGGCCGCACAGGAAGATGAGAGACACGAAG10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.22988505747126436No Hit
ATCAACGCAGAGTACATGGGAAGAGTGGTATCAACGCAGAGTACATGGGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACGATCTGGAGTGGAGCCTCAGCCCTCCAGCTCCTTGGCCTTCTGCTCCA10.22988505747126436No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 28bp)
GATTACATGGGAAGCAGTGGTAACAACGCAGAGTACATGGGAAGCAGTGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTG10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTAAGTATTGAGCCAAACAATTCCCGATCCAATCACTTTTGTCATGCGGT10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGCTGTCTCTTATACACAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTACCTGTCTCTT10.22988505747126436Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTGGAATTAAAGGTGAAACACAGCCAATTACTTGTATCATCTACTCA10.22988505747126436No Hit
TGGGGCGCGGTAGGGAAAGAGTGTATCTCGGTGGTGCCAATCCCATGTAC10.22988505747126436No Hit
GTAGCCATGAGGGTCCTCGCCCGCTGGAATAATTGTTTCAACCGTCACTT10.22988505747126436No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTGAAAACAAAGCTA10.22988505747126436No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA10.22988505747126436Illumina Single End PCR Primer 1 (100% over 26bp)
GAGCTCATCGAAATCCAAGAAAACTTCGCGCCGAATATGATCACCGCCTT10.22988505747126436No Hit
ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGATACATGGGAAGCAGTGGTATCAACGCAG10.22988505747126436Clontech Universal Primer Mix Long (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAACAC10.22988505747126436No Hit
GTGTAGGAAAGAGTGAGATCTCGGTGGTCGCCGTATCTCCCATGTACTCT10.22988505747126436Illumina Single End PCR Primer 1 (95% over 22bp)
GCGTCCGATCACGGACGCCTCGTGTTCGGCGCGGCTGAACTGGGACAGTG10.22988505747126436No Hit
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 28bp)
CAGTAACAGACACAAAAACATTCAATACACTAACATATACACATCTCAGA10.22988505747126436No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTTCGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
AGCTAACACAGTGCCTTATACATAGTAGTCTCTCAATTAAGAAGATTTGC10.22988505747126436No Hit
TCCCTACACGACGCTCTCCCGATCTGTACTGGTATGCAGACCCGGGAGAG10.22988505747126436Illumina Single End PCR Primer 1 (96% over 25bp)
AAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG10.22988505747126436No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGGTAGTTCGGGCACCCCATGTACTC10.22988505747126436Illumina Single End PCR Primer 1 (96% over 26bp)
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTCTCCCATGTACTCT10.22988505747126436Illumina Single End PCR Primer 1 (97% over 39bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATATGCTTTG10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGCTG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTGCGCAAGCAGCGCTGGGGCCGCCCCGATTCGCTGCGTGCCGCGAAGCG10.22988505747126436No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 27bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTAGACGGTCGCCGTCACCCATGTACTCTGCGTTGATACCACTGCTTCCA10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.22988505747126436Clontech Universal Primer Mix Long (100% over 23bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGGGTTTTTA10.22988505747126436No Hit
GCACCAGCATCATCGCCAGCACCAGCGCAAAGCTCAGTGCCAGTTGGGCC10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTTCGTCATCGTGGTCGCGCTCTCGCGCGTGGGGCAGACGAGGATGGG10.22988505747126436No Hit
CTCCACGGCGGCGGCCACGGTGTGGCGGTCGACGGGCGCGTTCCGCGCGT10.22988505747126436No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACCAAGGGTGCCC10.22988505747126436No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GCAGGGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.22988505747126436Clontech SMARTer II A Oligonucleotide (95% over 23bp)
ATGCACATGTTAGATATTAAAGTACTGCGCAACAACACTGAAACGGTGAA10.22988505747126436No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CCCATAACTTTTGGCTTGTTTAGAGTTAAGGGAGCCATAACCAGTCAAAA10.22988505747126436No Hit
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAGCGACCACCGAGACTACACTCTTTCCCT10.22988505747126436No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTGAAACC10.22988505747126436No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATACCGTTTTAA10.22988505747126436No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATATGCGTTTATACCC10.22988505747126436No Hit
GTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTAACAACGCAGAGTAC10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCAC10.22988505747126436No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
TCGCGGGAGTCGGCAGCGGAGCGCCCGCGCTGGGGTCGGGCTGCCACGGG10.22988505747126436No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CGTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTA10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAATATACGCCACTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATA10.22988505747126436Illumina Single End PCR Primer 1 (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
CCTTCTCTCTGAGAAAATGATTAGGCACACACGAAGAGCTGTCTCTTATA10.22988505747126436No Hit
CAGCCCACAAAATATAAAAAATAAACCTAAAACAAAAACAAAACAACTAA10.22988505747126436No Hit
CAAAGTAAGTGTATGCGTGACCACCTTAATCAAAATCATTTTTTTAATAT10.22988505747126436No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCAAAGCTTACCC10.22988505747126436No Hit
GTACATGGGTGATACGGCGACACCGAGTCTACACTCTTTCCCACACGACG10.22988505747126436No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.22988505747126436No Hit
CTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGGATAGA10.22988505747126436No Hit
ATCCATTTTCTATCCATGGTGCGGAGCATCATCATACGTTCGAACAGGCG10.22988505747126436No Hit
TCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACC10.22988505747126436Illumina Single End PCR Primer 1 (96% over 31bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGATGTCTCTTATACAA10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGACACGAGATCTAAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAACAGAAGTGACCGTTAATAAAAATGACGGCAGTTATAATCAACAC10.22988505747126436No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436No Hit
GCTCGTGAGGGTGTATCGGATACGGCGGCGGAACTTGCGGCGCAGGCGGA10.22988505747126436No Hit
TACTGACAAGGAGAAAAGAGTTTCTGTTTTGTTTTTGCTTTGATTTTTAA10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCACC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10.22988505747126436Clontech SMART CDS Primer II A (100% over 24bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGTCAGTTCTTTGCATTATTTTTTTTCTTCGTTGTTAACAAAAGAAAAGA10.22988505747126436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT10.22988505747126436No Hit
GTAGTAAGACTTTCCTATTTGGTTTCGAAGAAAGTTACGGATACTTAGTG10.22988505747126436No Hit
ATTCTAAAACAATTGTTTTAAATTTTTCTTTTCCATATAATTGAATAGCT10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GTGTTTAGACTGTTCACATTTAATGTGATTATTGCTGTCTCTTATACACA10.22988505747126436No Hit
CCCATGTACTCTGCGTTGACCCATGTACTCTGCGTTGATACTGTCTCTTA10.22988505747126436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.22988505747126436No Hit
GTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTGTC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.22988505747126436No Hit
TCACAGTTCACTGTAACCTTGATCTCCTGGGTTCAAGGGATCCTTCTGCC10.22988505747126436No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACACATGTTACCA10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCTGCGTTGATACCACTGCTTCCCATGTAC10.22988505747126436Clontech Universal Primer Mix Long (95% over 24bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAATT10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTCTCTTATACACATC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTAC10.22988505747126436No Hit
ACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGTGTACAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCGAAGATCGTAGCGGGTCTGCTTGAGGCCGATGGTCACGGCCTCTTCC10.22988505747126436No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGTTTGTA10.22988505747126436No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTTGATA10.22988505747126436No Hit
ATTCGGTTATGGCCTCACAAAAATCAAGGGGAAGGTCATTTTGCCGCAAA10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAAACGGCGAG10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
GGCTTCAGCAGGATCAAGCATCAGAGGTTCACCCCTGCGGCGATCACCGC10.22988505747126436No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
TCCCTACACGACGCTCTTCCATCTCTGGTACCTGCAGAACTTGGACAGGT10.22988505747126436No Hit
GGACAATATTGTCGAATTGCTGTTTGTGCTGGCACCTATTTTCCTGTTGG10.22988505747126436No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCATAGTACATGGGACGCCTGTCTC10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCACGAGGCCATTGCCGGCCTCAACCCCGAGGACTACCGCGACGCCA10.22988505747126436No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACCACTGCTTCC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGTGAACGGCGACACCGAGATTACACTCTTTCCCTACACCTGTCT10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTGACTGTCTCTT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTTTCCCTACA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCCGGCCCGAGGGGGACGCGGTGCTCGCGAGGGACTGAGCGGCCCGTG10.22988505747126436No Hit
GCTTAGGTGGAAGGCGAAGAAGGCCTCCTTCCGGGGGGGCCCGAGCCATC10.22988505747126436No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTTTAAC10.22988505747126436No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAGGGTTTATACC10.22988505747126436No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTTTCA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10.22988505747126436No Hit
CTCTGCTTGCTGAGCGGATGGCGGACGCCGGCGGTCGTCGCAACTCAACG10.22988505747126436No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCCCATGTACTCCTGTCTC10.22988505747126436Illumina Single End PCR Primer 1 (96% over 30bp)
CCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAGAAAAAACTCCGTTTGCAT10.22988505747126436No Hit
CGTCGCGCCGCGCTTCGGCCGTGCGCAGGCCCAACGGCTCGCCGCCGACG10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGATACGGCGAACGATACC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTTAAACA10.22988505747126436No Hit
TTTCAATGCTAAATCACCGGCGATCATCGCCGTTTCGCCAGGAGTTAGCG10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGATCTACCTCTTTCCCTACACGACGCTCTTCCGCGTCCCA10.22988505747126436Illumina Single End Sequencing Primer (96% over 27bp)
CGTGTCGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACCCGGCCCCCTGCAACGCTCTCGCGAAGATGAGGACAGCGATGTCCGAC10.22988505747126436No Hit
CGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATT10.22988505747126436No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGATTACATGGGAAGCAGTGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGCTGTCTCTTATACACAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGGGTTTAAACCA10.22988505747126436No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTACC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
ACTCGGCATCAGGTGACTGCGATTTGAACGCAGAAAGTCCTTCTCCCAAA10.22988505747126436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAATCGTTTAAA10.22988505747126436No Hit
ACATGGGTGAACGGCGACACCGAGATTACACTCTTTCCCTACACCGGTCT10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAAG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT10.22988505747126436No Hit
GAGTACATGGGTGTACGCTCGAGTCTACACACTTTACCAGCAGGTACCAG10.22988505747126436No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGT10.22988505747126436Clontech Universal Primer Mix Long (100% over 23bp)
TAATAATACCTCCGAACACACCTGTTTGAAGTGTAGGAATCCCTAATACA10.22988505747126436No Hit
CCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAG10.22988505747126436No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACACATCT10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCAAGCAT10.22988505747126436No Hit
GGTCCTGAAAAACGAGCGTCGTGTAGGAAAGGGTCCGACCATGTACCTGT10.22988505747126436No Hit
CTCTTCCGATATACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAG10.22988505747126436No Hit
CCACCGCGACTGCCCTGATGCCCTGGGCATCATCTCGACCACCTTCGGCG10.22988505747126436No Hit
TTACAGGAGTGAGCCACCATGCCTGGCCAAATTTTTAACCTCAGTCAAGC10.22988505747126436No Hit
GATAACATATCAACAAGACCTATCAAAGAATAGAACATGTCAAATATAAA10.22988505747126436No Hit
TCGATCTGGAGTGGAGCCTCAGCCCTCCAGCTCCTTGGCCTTCTGCTCCA10.22988505747126436No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGACTGTCTCTTATAC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGGAA10.22988505747126436No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGCTGTCT10.22988505747126436Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGAA10.22988505747126436Illumina Single End PCR Primer 1 (95% over 22bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTATCCCTTTTTTTGGCCTGTTGGTACCATAAAAGGGATAGA10.22988505747126436No Hit
CTCTGCTTGCTGAGCGGATGGCGGACGCCGGCGTTCGTCGCAACTCAACG10.22988505747126436No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCCATCCCGTGCGCGACTTCGACGTCCTCATCGTCGGCAGCGGACTGG10.22988505747126436No Hit
GAGGAGATTTCTCCTTATGATAGTCAAAGCATTTTTGACTATCATAAGGA10.22988505747126436No Hit
CCCGGAACGAGCACTGATGCACGAGGCTGCGGACGAAGGCGCAATCGCGG10.22988505747126436No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTT10.22988505747126436Clontech SMART CDS Primer II A (95% over 21bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.22988505747126436No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGCTGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
GAGCCGCTCCGCTTCGTCCATGTAGTGCGTCGTGAGCAATATGGTGCGGC10.22988505747126436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGCGTTGATAACAC10.22988505747126436No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGACCACT10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GCTGGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG10.22988505747126436Illumina Single End PCR Primer 1 (100% over 30bp)
CTTGGCAAGCAGTGGTATCAACGCAGAGTACTTGGCAAGCAGAGGTAACA10.22988505747126436Clontech Universal Primer Mix Long (96% over 27bp)
GGATATCCCAAGCTCCCGACACACCGCGGCGATGTCGCTGCCGTCGGCGA10.22988505747126436No Hit
GAAATTAGCTGTTGACGGTATAAACTAACGAAATCGAGTATACTGTAACA10.22988505747126436No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.22988505747126436No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTAAAAAAAAA10.22988505747126436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)