FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA235.287356321839081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG143.218390804597701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT132.9885057471264367Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132.9885057471264367Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC81.839080459770115Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC81.839080459770115Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG71.6091954022988506Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC71.6091954022988506Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG51.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT51.1494252873563218Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT40.9195402298850575Clontech Universal Primer Mix Long (96% over 26bp)
CCGTCGACCTGCACTTCGCCGACTGGCGTCAGGGCGATCAGCGTTACTTC40.9195402298850575No Hit
AGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCT40.9195402298850575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.9195402298850575Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.9195402298850575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC30.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGA30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
CCATCGCCCGGCGGAAGGCGCTGACTCTCCAGAGTTCAGGCTCGCCGAGC30.6896551724137931No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTC30.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAGCCTGTCTCTTATACACAT20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GATGCCCTTGGCGTCGTGCTCCCAGCCGCCGGTGTCCACCACGGTGAACT20.45977011494252873No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.45977011494252873Clontech Universal Primer Mix Long (95% over 22bp)
TCGACGTCCAGTGGAACACCCCACACCTGAGCACGCAGGGCGTGCGCGAT20.45977011494252873No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.45977011494252873Clontech Universal Primer Mix Long (95% over 22bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCCACAAGCTGCGCGAGATGGCTTTCCGCGGCGAAGTCCCCGGCATGACC20.45977011494252873No Hit
GTACTGGGTGATACGCGACCACCGAGATCTACACTCTTTCCCTACACGAC20.45977011494252873Illumina Single End PCR Primer 1 (97% over 37bp)
GATCATGATGGTTTCAACGGTATAGAAAAAGAATTTTCAGTAAATCCTAA20.45977011494252873No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCACGACATGGTTAAACATCGCTTCGACTACTCCTTTTCCGGCCTTAAG20.45977011494252873No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTATACACA20.45977011494252873Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
CCGCTAACCGATTCCAGCAAATAGTTGTAGGTACAGCCCAAGGATTTTGG20.45977011494252873No Hit
GGTTTATTGAGAGCGACCGCGCCAAACCTGCCCTGAGATTTAAGCCTAAG20.45977011494252873No Hit
CAATAGATCCGTCAGCCTTGTTGTTCAAGGCTGACGGATCTATTGGCCAT20.45977011494252873No Hit
GGTATCGGCAGCTCCCAGTAAACATGAGATCGGAAGAGCTGTCTCTTATA20.45977011494252873No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCATTCACTTTC20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCGCCATGTGCCACAACGCGGCCGCGGCCGGCGGTGCGCTCACCCGCGGC20.45977011494252873No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.45977011494252873Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.45977011494252873Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGC20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGCACACCAGCCAGCTGACCCCTTAACTGGATTAAGGAAGACCTAGAA20.45977011494252873No Hit
GTTCCGCTGGCCGACAGCATCGAAGCGTTCACCAAGATCGCCGACGGTGA20.45977011494252873No Hit
CCTCCGGGCAGATGCAGGAGGCGATGCTCGCCATCTTCCGCGAGGAGGAG20.45977011494252873No Hit
GTGTCGCGCATGATCAGCGGCGGCTCGGCCGGGTTGATCACGATGATGGC20.45977011494252873No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.45977011494252873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCTTTGATACCACTGCTTCCCATGTAATCTGCGTTGATACCACTG10.22988505747126436Clontech Universal Primer Mix Long (95% over 23bp)
TTATAATATTGACGATTCTGAACATATTGATTATGAACAGTTCATCAATA10.22988505747126436No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAATGGTCGGCGGCCACCCAGAA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGATACGCAAATGTATTAGGGATTCCTACACTTCAAACAGGTGTGTTCGG10.22988505747126436No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGACGGCGG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCGAG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGCTGTCTCTTA10.22988505747126436No Hit
ACTCCAACCACGCCCTCAACTACGGCTTCGACCGCCTGCGCTTCGTGCGG10.22988505747126436No Hit
GAGTACATGGGCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCT10.22988505747126436Illumina Single End PCR Primer 1 (96% over 29bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTTACAAACCATACC10.22988505747126436No Hit
GCCAAGTACTCTGCGTTGATACCACTGCTTGCCAAGTACTCTGCGTTGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAACCAGGGTTACC10.22988505747126436No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTCTTCCCATGTACTCTGCGTT10.22988505747126436Clontech SMART CDS Primer II A (95% over 22bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTACCG10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGAAGCAGTGGTATCAAAGCAGAGTACATGGGAAGCAGTGGTATC10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAAGGGTAGCAGT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGTGCCGACCCCCGAGATCTCACACTTTCCCAACAGCAACA10.22988505747126436No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACTAAATGTTAACAGTAGCAATGCCAAAAGGACGTATATTTGAAGAGGCT10.22988505747126436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACTGCATCCCA10.22988505747126436No Hit
GCCAATAGCACGCCTTACGGATTGGCAGCAGGCGTTTTTACCACCAATTT10.22988505747126436No Hit
GTCGGCATGATGATGAGGCCCGGCCACGTCGAAGTAGCGCATGATGTTTC10.22988505747126436No Hit
CCGTACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTATCTGCG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTGTC10.22988505747126436Clontech SMART CDS Primer II A (96% over 25bp)
TGTTGATTATAACTGCCGTCATTTTTATTAACGGTCACTTCTGTTGAACC10.22988505747126436No Hit
GTCAAGGCAAAGTCCGGTAATGGCGTTCTGAATACCAGAGATAATTCTCT10.22988505747126436No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAATTCGGCGACCCCCGAGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGTCGGACCCTTTCCTACACGACGCTCGTTTTTCAGGACCCTGT10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCGGGGTCGCACAGGCCGGCGCCGGCAATCAGCGTGTCTTCGACGTG10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTACCAACGCAGAGTACATGGGAAGCAGTGG10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACCTGTCTC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CAGCTAACCGATTCCAGCAAATAGTTGTAGGTACAGCCCAAGGATTTTGG10.22988505747126436No Hit
GTACTTGGGCCTGGAGTGATCCGGGCCCATCCTCGTCTGCCCCACGCGCG10.22988505747126436No Hit
CATGTAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTAGTTTATACCACAGCTTCCCATTTAC10.22988505747126436No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACCAATGATGATACCCAGGGCGCGATCGCGAATTTCCACCAGGTCGTACA10.22988505747126436No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCTACCTCTTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
GGGGTATGGTCTAACAGGAGAGAGAGAGAAAGAGAGAAAGAGAGACACGT10.22988505747126436No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCTGTCTCTT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAACAATGGGAAAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.22988505747126436Clontech Universal Primer Mix Long (100% over 23bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA10.22988505747126436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACGACATCCCA10.22988505747126436No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCT10.22988505747126436Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGTGGTCGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTATCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
AACTTATATTGGTCAGCATAAAAAAGATTATTATGACTATTATTACAAAG10.22988505747126436No Hit
GTATCAACGCAGAGTACATGGGGTGCCCGAACTACCTCTTTCCCTACACG10.22988505747126436No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
TTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCAC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCTGCCCATGTAC10.22988505747126436No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGTACTTTTT10.22988505747126436Clontech SMART CDS Primer II A (100% over 24bp)
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCGGTGGTATCAACGCAGAGTACATGGAAGCA10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 28bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAGAAAGCGTTTATACA10.22988505747126436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAGCGTTTAT10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGCTGTCTCTTAT10.22988505747126436Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGATA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGATAACA10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTGGTTTGCTGCCTTCTTTGGGAGAAGGACTTTCTGCGTTCAAATCG10.22988505747126436No Hit
AGATAGGTCATCTTTGCGGCAAAATGACCTTCCCCTTGATTTTTGTGAGG10.22988505747126436No Hit
GCTTACCCATGTACTCTGCGTTGATACACTGCTCCCAAGTACTCTGCGTT10.22988505747126436No Hit
GCACGGCTCGCCGTACATGATGCTGCTGGCCACGGGGAGCAGCTTCTGCA10.22988505747126436No Hit
GCACCCGCTGGTCCCTGATGCGCCGTGAACCGACCGGATGATGCAGCCGG10.22988505747126436No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCCTGTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGGTCTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACGCGTTGATACCAAA10.22988505747126436No Hit
TTCCTTTATTGCTTGACTGAGGTTAAAAATTTGGCCAGGCATGGTGGCTC10.22988505747126436No Hit
GGCGAACGATACCTCTTTCCCAGCAACTCCAGTCCTTGCATCATGAGATC10.22988505747126436No Hit
ACAAAGTAAGATCCCCATCTCTACAAAAAAAATTTAAAAATTAGCTGGGC10.22988505747126436No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
GTAGGTCCTGACACAAGGTTAGAATTCTAGCTCTTCCAGAGTGGTATCTC10.22988505747126436No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10.22988505747126436No Hit
TCTACACTCTTTCCCACACGACGCTCTTCCGATCTCTGGTACCTGCAGAA10.22988505747126436Illumina Single End PCR Primer 1 (95% over 23bp)
CAGCAAATCTTCTTAATTGAGAGACTACTATGTATAAGGCACTGTGTTAG10.22988505747126436No Hit
GCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCCATCGGCTGAGGGCGGCTGGGATGAAGACGGTGGCCCAACTTGCCGAA10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTGATACGGCGACCACCAGATTCACTCTTTCCCTACACGACG10.22988505747126436Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCAGAGTACATGGGATACGCGCTCTACATCTTTCCCTACACGACGCTC10.22988505747126436No Hit
ACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACTGCGTTTTT10.22988505747126436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG10.22988505747126436No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATC10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGCGTTGATACCACTGC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAATCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTG10.22988505747126436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.22988505747126436No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGTGATCGGCGACCACCGAGACTACACTCTTT10.22988505747126436No Hit
GATCGGAAGAGCGTCGTGTAGGGAAGTGGCGTATATTCCTGTCTCTTATA10.22988505747126436Illumina Single End PCR Primer 1 (96% over 25bp)
ATCTAACTCTTTCCCTACACGACGCTGATCGGAAGAGCGTCGTGTAGGGA10.22988505747126436Illumina Single End PCR Primer 1 (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGCTTCCCATGTACTCTGCGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 23bp)
GAAGAGGCCGTGACCATCGGCCTCAAGCAGACCCGCTACGATCTTCGGGC10.22988505747126436No Hit
CTCTTCGTGTGTGCCTAATCATTTTCTCAGAGAGAAGGCTGTCTCTTATA10.22988505747126436No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
GAACAGCAGGAAGCGTCACACCCCGGAGCAGGTCGTCCGCAAGCTCGGGC10.22988505747126436No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.22988505747126436No Hit
CTTCCACTCTGTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCG10.22988505747126436No Hit
CAGACTTTTTTTTTTTTTTTTTTTTGGGTTTATACCACTGCTTCCCAATT10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GGGAGCTGATCGAAGAGTTCAAGCGTGCCGGCCGCACCATATTGCTCACG10.22988505747126436No Hit
ACTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTGTAGG10.22988505747126436Illumina Single End PCR Primer 1 (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436No Hit
GCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACTGCTTCCCAT10.22988505747126436No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGATCGGCGACCACCCAGAAAAAAAAAAGTTACCCCGTGGC10.22988505747126436No Hit
GCCCAACTGGCACTGAGCTTTGCGCTGGTGCTGGCGATGATGCTGGTGCC10.22988505747126436No Hit
GTACTTTTTTTTTTTTTTTTCGTTGATACCACTGCTTCCCATGTACCTGT10.22988505747126436No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCTGCGTTGATACCACTGCT10.22988505747126436Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTGGTGTTATGACTGATAAAGCCCAGCGCAGCTAACCCGAGCAGGATCAG10.22988505747126436No Hit
ATCAACGCAGAGTACATGGGTGATCGGCGACCACCGAGACAACACTCTTT10.22988505747126436No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGTACACT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GAATCACTCTTTCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAA10.22988505747126436Illumina Single End PCR Primer 1 (96% over 26bp)
CGCATAGTACATGGGATGATACGCCCACCGAGTCTACACTCTTTCCCTAC10.22988505747126436No Hit
GCTGTACGGACCGCCACCCACGTCAGATGCGGGCCTGGACCTCCTGGTCC10.22988505747126436No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG10.22988505747126436No Hit
GGGAAGCAGTGGTATCAACGCAGATAGTACATGGGAAGCAGTGGTATCAA10.22988505747126436Clontech Universal Primer Mix Long (95% over 24bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
ATTCAGGATCAGGCCGCCGGGTCGCGGATGTCCCAGCGGATCTTGTCGAT10.22988505747126436No Hit
GCGACCACCGAGATATAAACACTTTCCCAACACGACGCTCATCCGAAATC10.22988505747126436No Hit
CAGCCACGGTGATCCCGTTTTAACGTAAAAATTCACGTGATGGAAAAAAG10.22988505747126436No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTGAGTTTATACCACTGCTTC10.22988505747126436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAATGGTTTTAAACAAAT10.22988505747126436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCAAGTACTCTGCGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 22bp)
GTCGTCCATTGCCGCGTCAAAGTCTTGCAGGAATGCCTCCGCCTGCGCCG10.22988505747126436No Hit
GTACTTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATACACAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 28bp)
GTCCCGTTCAAGTCCCCCCTGTTCTACGCCGCTGTCTCGCCCAGGGCTCT10.22988505747126436No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATTACTC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
TTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT10.22988505747126436Clontech SMART CDS Primer II A (95% over 22bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCT10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGATACCACTGCTTC10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
GGCAATGTCAGGGTCGGAAAAGACAATGGCCAACGCAACACGGCGCCGGA10.22988505747126436No Hit
ATTGTGGCCCTATTCTTAGGTGCATGTGCGAGCGGTGGGGAGTCAGCTAG10.22988505747126436No Hit
CGCGCAAGGCCGTGCCGCAGGAGTCGCTGGTCGTCGGCGGCGAGCCGATG10.22988505747126436No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCAA10.22988505747126436No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGGACAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGGAGGG10.22988505747126436No Hit
GTGTAGGGAAAGAGTGTAATCTCGGTGTCGCCGTTCACCCATGTCTGTCT10.22988505747126436No Hit
TTCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.22988505747126436Illumina Single End PCR Primer 1 (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
ATCGGCATCGATGCAGCCGGCCAAGTGCATCGGGTTATTGGCCACAAAGC10.22988505747126436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTC10.22988505747126436No Hit
GTGCTCAAGGCCGGCACCCTGGCCCCCGCGCCGCGGCACGGGCCGCTCAG10.22988505747126436No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGT10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
ACACCATCCTGGGCAGATCCTCCCAATGTAGAATTCAAATTTCCAATCTC10.22988505747126436No Hit
CCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTACTCTGCGTTGAT10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACA10.22988505747126436No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGCGTTGATA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTACACAGGGTACCCATT10.22988505747126436No Hit
ACAGTTATTTCCACAAAGAAAATAAAACAATTTCGCAATGCTTTTGGAAA10.22988505747126436No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACACATCT10.22988505747126436Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAATGGTCGGGGCCCACCCAGAA10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
TAACACCCAGGAAAGCAAGCGGTGAGGGAGGGCTCCTGGCTGCCTGGTTG10.22988505747126436No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTT10.22988505747126436No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
CAATAATCACATTAAATGTGAACAGTCTAAACACCTGTCTCTTATACACA10.22988505747126436No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAGATACGGCGACCACCGAG10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTGCCACGAGATCAACACTAATTCCAACACGGAGCAGCCGAT10.22988505747126436No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAATTAC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAATACATG10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTC10.22988505747126436Illumina Single End PCR Primer 1 (100% over 45bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAGTTTAATTATATGAA10.22988505747126436No Hit
GTGTAGGGAAATATTGTAAAAAAGGTGTCGCCCTTAAACCAAATAATTAT10.22988505747126436No Hit
GTATATCCCATGTACTCTGTATCAACGCAGAGTACATGGGATATACGGCA10.22988505747126436No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10.22988505747126436No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAGTGTAATTTTTTTTT10.22988505747126436No Hit
GCACCCTCTTGACCTGGTAAGCTTACAGAAATTGTTTTTTCTTGGTAGGT10.22988505747126436No Hit
ATGCACCACAGCGAATATCTCTCCATTCTTAGTGATCTACCTCACCTTTT10.22988505747126436No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.22988505747126436Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGGAAGCGGGGCGCGGCGCGAGTATCCGGCATTCTGCTTTTGATCTT10.22988505747126436No Hit
CTTTGTTACGGCGGCTTTTCAGTTCTTCAGTTTCAACAATCAGCGCGCGT10.22988505747126436No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTACTC10.22988505747126436Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGAAGCAGTGGTATCAACGCAGATTACATGGGAAGCAGTGGTATC10.22988505747126436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGTCAGAAACTCTGGGCCCTGAGATCGGAAGAGCGTCGTGTAGGG10.22988505747126436Illumina Single End PCR Primer 1 (100% over 24bp)
GCCGTGTCCTGGCTGGTGACCGTCACGCTGCTGGTCGGCGCTGTCGTCAC10.22988505747126436No Hit
ATCGACGCAGACGCAAGTCTCGAAGATCGCCACTGTTACAGTATACTCGA10.22988505747126436No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATACAC10.22988505747126436Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)