FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT84.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63.296703296703297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTATGACAGAAGACAATAAGCAAGACACATCATTTCCGTCCGGCGGCAG42.197802197802198No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC42.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
TAATATAATTTTCCAAATGTTGGCTACTTGTTTAATTTTTTAAACATGCA31.6483516483516485No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
AGAATAAGCAGTTCTCCCACACTTCTGGAGAACAGCCTGGAACAAACTGT31.6483516483516485No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT31.6483516483516485No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31.6483516483516485Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31.6483516483516485Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
GGCATACCTGCTCGGCGCGCCTTTTGCTGTGGGGGCCGTGATCTTCGGCT31.6483516483516485No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA31.6483516483516485No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GAGCCGAAGAGAATATAGAAGACATCCCAAAGGAAGTGCTGATGGACTGT21.098901098901099No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CTTCAGCTTTGTAAGGTATTCATGTTCCTAACAGGTAGCCCACTTGGTGC21.098901098901099No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CAATTCCATTCGAAGATGATTCCATTCAATTCCATTCGATGATGATTCCG21.098901098901099No Hit
GTTTCCGGGGAGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGG21.098901098901099No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTTTGCTGTTGTTGCCCAGGCTTGCCTTGGACTCCCGAGCTTGTGTG21.098901098901099No Hit
GTGGACCCGTTACGAGGATCCCACTCCGTACCTCGAGGAGCTGGCTGGTA21.098901098901099No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTTGTAGTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAA21.098901098901099No Hit
GCGATGCGGCGGCGTTATTCCCGGAATCTGCCCGGCGGGTCATGGGAAAA10.5494505494505495No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GCCTACAACAGGTCAGTAGTGATGCTCCGATCAGAAAAACAAGATACAAA10.5494505494505495No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACT10.5494505494505495No Hit
CTTCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GCCTTGGAGAGCGACCAGATTCCTCACCCCACTCAGGAGGAGCCAGGGCA10.5494505494505495No Hit
TCGTTATAGCCTTTAAAATCATATTCCCAGTGACCAAAACCAATCATTTG10.5494505494505495No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA10.5494505494505495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTA10.5494505494505495No Hit
CCATATGATACTCATCTTGTTTTCTGAGAATGTCTTTCAGCTGCAGATGA10.5494505494505495No Hit
AAGAAACACCAAAGCCAGTAATCTAATATAAAGTTCCAGAAAAAGGGAAT10.5494505494505495No Hit
TAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTT10.5494505494505495No Hit
CACCACCACCACGGCTGCCACCAAAGCCACCACGACCACTGAAGTTTCCT10.5494505494505495No Hit
ACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAGAGTACATGGGAAGC10.5494505494505495Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC10.5494505494505495No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAAC10.5494505494505495No Hit
GGCCAGGCTGGTCTTGGAACTCCTGACCTCAGGTGATCCGCCTGTCTCGG10.5494505494505495No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAC10.5494505494505495No Hit
GTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACA10.5494505494505495No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTATGTCACTCAT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
AGGTGGAACCGTCCCAAAATGTCCCTTGCTCAGAAGAAGGATCGGGTAGC10.5494505494505495No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTACTCTGCGT10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCAAGCACAAACACCAAATGTCTGAACCTCCGGATCCACTCGTACAGAG10.5494505494505495No Hit
AGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCG10.5494505494505495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGATGTTA10.5494505494505495No Hit
GCCGGGGCCAATGCCGCGCTCGAGCTCAAGGACGCGCGGGTGAGGGTCAC10.5494505494505495No Hit
TTTTGAGAGAGCCGTTTTGAAACAATCTTTTTGAAGTATCTTCAATTGGA10.5494505494505495No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGGACCG10.5494505494505495Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
TGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCACCACGATCAA10.5494505494505495No Hit
GAATATCATAAAGAGATAGAATTTACAAAAAGAAAAAAGGAATAAAATGA10.5494505494505495No Hit
CATACACACCATGGAATAATACACAGCCATAACAAGAATGAAATCATGTT10.5494505494505495No Hit
GTCGTAGCGGTTGGGCACACCGTCGCCGTCGTTGTCCCTGCGGCCATAGC10.5494505494505495No Hit
CTTCGAGACCGTCAACCCCGCCACGCAGGAAGTGCTGGCCGAAGTCGCCT10.5494505494505495No Hit
GTAAGAAAATGAAACAAGTTACAATCTATGCCTCAAGAGAATTAGGACTT10.5494505494505495No Hit
GCATGGTACACAGGTTTATGTAATCCCTGAAGTCTTATAGATGCCATAGC10.5494505494505495No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGAAGTGGAATC10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
GTTTAAGTATGCATTGAGTACATGACCAACTAGAATTAAAGTAAGTGTAA10.5494505494505495No Hit
CGCAGAGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.5494505494505495Clontech SMART CDS Primer II A (96% over 26bp)
CTAGTTTACAAAGCTACTGTAGAGCTCGAAAGAGGGTGACAGGAATAGGG10.5494505494505495No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
TTTATACTTTTCAAAGTACCTACATATTCATTATCTCCCTTGAGCCTCAC10.5494505494505495No Hit
GGACAAGGTGAGAGGGCTCAGAGGGGACCTGTAGAAGGTATACACAACCT10.5494505494505495No Hit
ACAAAATATAGTATTTTCATCAAGGCCTGAGATTATTAGGGTTAAAGGTA10.5494505494505495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTTAAAAC10.5494505494505495No Hit
TACCAGTACTGATTGTCGAGCAAATCTGTGAATGGCGACTATGCGTCTAG10.5494505494505495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5494505494505495No Hit
AGCTTCTACAGTGGAATTAGGAGAATTGGATAAGAAATGAAGCACCCAGA10.5494505494505495No Hit
ATTATAGACTCACTGGTCACTTTTGTAAACTATAATCTCCCATAGCAATA10.5494505494505495No Hit
CTTATGCTCTAGTTTTCCTCCCCTGGTCTTCTGTTACTTCCATGTATACC10.5494505494505495No Hit
GGCATACCTGCTCGGCGCGCCTTATGCTGGGGGTGCCGTGAACTTCGGCA10.5494505494505495No Hit
ATATATGACACTTATCTAGAGATGGAGGGGGAGGTAGGAAGTCAGCTTGA10.5494505494505495No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.5494505494505495Clontech Universal Primer Mix Long (100% over 23bp)
CTCCGATGGTGGCCAGGCCAACACCCCCTAGGGTCTCCCACGAGAACACG10.5494505494505495No Hit
CTCCCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTCCAGAA10.5494505494505495No Hit
CCTTCTCTAGGAGTGGCTGTTTTCTCTTATAAAAGTCCAATACCATAGAG10.5494505494505495No Hit
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG10.5494505494505495No Hit
CATGGGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATAC10.5494505494505495Clontech SMART CDS Primer II A (96% over 26bp)
CATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACACCGACT10.5494505494505495No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
TTACCTAATGAGTTTGAAAATTTATATCTGCATATAAACATGCACATGAA10.5494505494505495No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC10.5494505494505495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAT10.5494505494505495No Hit
AGTTATTCTCTTGGTATGGAGGGCTGGGTTGTTTGTATTGAATGTTGAAA10.5494505494505495No Hit
CCACCATGTGAGGACACAGTGAGAAGGTGGCTGTTTGCAAACCGGGAAGA10.5494505494505495No Hit
CTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCT10.5494505494505495No Hit
GTGGAGCGCGATCTGGCAGGCCGCATTGTGGCCCTCGTCAGCCCCACCGG10.5494505494505495No Hit
GCGGATAAACGTAGCCCGACACCTTCCCCTGCTAAAGCCTTAGACAGGTC10.5494505494505495No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACAA10.5494505494505495Clontech Universal Primer Mix Long (96% over 27bp)
AGGTCCAGCTGTAAAATATCTCAGGTACCCAGTTAGATCCAGATATTCTG10.5494505494505495No Hit
GTGTGCGGAAGTGTTTTGTGTGAATAGTCTTCCACATTTGGGCTCAAAAA10.5494505494505495No Hit
GGGCAGCATCATGTTGATGCCATTTCAATAGTGACTTATTTCAGTCTACG10.5494505494505495No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC10.5494505494505495No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTATCTGCTAATTTCAGCATCATTAGATCTTTTTACTTCGACTATA10.5494505494505495No Hit
GAGTACTTGGAAGCAGTGGTATCAACGCAGAGTACTTGGAAGCAGTGGTA10.5494505494505495Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTCTCAATGTGGTCAGTGGGTTATTGGTGGTTTCATAATAACTGGGGA10.5494505494505495No Hit
GCTTCCCACGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
TCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG10.5494505494505495No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACAAGAGAATGAATAAATTCTGGTATATACATACAATGAAATATAC10.5494505494505495No Hit
ATATATAAATAGCTCTTCATTTAAAAATACAGTTCCCCAGGTTGAGGTAT10.5494505494505495No Hit
ATGCATACTTGCTTAGAATCCAAAAGGCAAAGAGCAGATGGGTTGAAAAT10.5494505494505495No Hit
ATGCATACTTGCTTAGAATCCAAAAGGCAAAGAGCAGATGGGTTGAAAAA10.5494505494505495No Hit
CATGGGAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.5494505494505495Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTACTCTGCGTTGATAC10.5494505494505495No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTT10.5494505494505495No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG10.5494505494505495No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCATG10.5494505494505495Clontech Universal Primer Mix Long (96% over 27bp)
GTCCTGAACAGAGGATCGATGGTCCAAAGAGGCAAAGGTAGCAGCTTCCA10.5494505494505495No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCATCGT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCCGTACTGTGCGTTGATA10.5494505494505495Clontech Universal Primer Mix Long (96% over 27bp)
GTTCGAAGGTGATCCAGACGCAAGATGGCTGTCCTCTCTAAGGAATATGG10.5494505494505495No Hit
GTGTGGTAATGAGAGATCCAAACACCAAGCGCTCCAGGGGCTTTGGGTTT10.5494505494505495No Hit
GGTATCAACGCAGAGTACATGGGAGTAGAGTTACTCCATTGGAGGGCACG10.5494505494505495No Hit
GTACAAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)