FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94.945054945054945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATCTGTCTGCGCTTTTCGGGGTTCATGCCACGGAGCGCGGACGTGTAG42.197802197802198No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC42.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTT31.6483516483516485No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC31.6483516483516485No Hit
ATATTTTGCACTCGAATTAGACAATAATGTGGCTCTTCCTACTAAAGCAG31.6483516483516485No Hit
CACCGAGCAGGTTGCCGAAGATGATGTGATTCAGGTCCGTGTGCGATGGC31.6483516483516485No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31.6483516483516485Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATCACAAAATCACATGCAGCTGAAGGAAAAGAGTTGCACTGAAGCC31.6483516483516485No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA31.6483516483516485Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGA21.098901098901099No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT21.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT21.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
GAATCTAATGGAATCATCATCGAATGGAATCGACCGGAATCATCGAATGG21.098901098901099No Hit
ATGCAAGGCGGCCTGGTAGGTAGCGCAACCACCGACGATAACGGGTACGT21.098901098901099No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.098901098901099Clontech Universal Primer Mix Long (95% over 22bp)
TACAAATATGTGTGTGTGTATAATCAGCAGATATTTAGAGACATGCCTGG21.098901098901099No Hit
GTGTTTCTCACAGAGGGGGATTTGGCATGGTCATAGGACAATAGTGGAGG21.098901098901099No Hit
ACAGAGACCCTGCATTTTGTGAGCATTAACAAGTATCGTGTCAAATACGG21.098901098901099No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC21.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
AAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCG10.5494505494505495No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAATGCAGAGTACAT10.5494505494505495Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTGTATTCTGTGCATTATTTTGTCATGATCTCAATTCTCTTCTTTC10.5494505494505495No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG10.5494505494505495No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTCC10.5494505494505495No Hit
GTACTCTGCGTTGATACCACTGCTTCCAAGTACTCTGCGTTGATACCACT10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGAGTACATGGGAAGCAGTGGAATCAACGCAGAGTACATGGGAAGC10.5494505494505495Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGAAACTGGTCAGTAATAAACTACCCTTGGTCAGTAATAATCTACCTGCT10.5494505494505495No Hit
GCCAAAGCTAACCCGGCGGAGGTCCTTTGATCCCGGGGCCACCGCCCGCT10.5494505494505495No Hit
ATCTATATCTGAAAAAATCATAGAAAATTGCTGGGTTTAGCTCTCAGCAG10.5494505494505495No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTATAGGTCTCTGAAGTTCTGTTTATTTTTCTTCATTCTTTTTTCTT10.5494505494505495No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGGCTGGGCAACTTAGTGAGATCCCTATCTCTACAAAATTTTTTAA10.5494505494505495No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GCCCGCTCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCC10.5494505494505495No Hit
GTTACGGATATGCCGCCACCAATCCCCTCGCGCTAGCCGATCCACTGGGT10.5494505494505495No Hit
ACGTCGTTCCGCCGCCGCCACCGCCGCGCCGCTACTACGCGCCGCCGCCT10.5494505494505495No Hit
GTATATTGTTACAGAAAAGCAAATTAGCAAACAAAGTGGGCCAATGTTAT10.5494505494505495No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
CCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG10.5494505494505495No Hit
GTCTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAA10.5494505494505495No Hit
GTATCAACGCAGAGTACATGGGGTGGTGAGGTTGATCGGGGTTTATCGAT10.5494505494505495No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAAGGAAACAGAAATAAACTGAATAATACGTGGAAACAAAGCTGTGACT10.5494505494505495No Hit
GGCTTATTCTTGTCAAATGAGGATATTTTTGTGCTGTATTTTGAGCTGAT10.5494505494505495No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
ACATCATTTGCATCTTTTGAATAACTGTCTTTGGGATCCACTGTGGTGAC10.5494505494505495No Hit
ATAAAAAGCCAATCTATAGTGGCTTAGGGGCAAAGAAATGGAGGCAAGGT10.5494505494505495No Hit
GGGTTGAGCTTGGAAACAACTGATGAGAGCCTGAGGAGCCATTTTGAGCA10.5494505494505495No Hit
GTATTTCTGAATGACAATCTTATCCACGGAGTCATGGTCGTCAAAGGTTA10.5494505494505495No Hit
AGTCAGCAGAGAGAAGCACTTTTGGAGTAGCATGGCAGGGGCCCCTGAAA10.5494505494505495No Hit
NTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA10.5494505494505495No Hit
CAAAGCGAAGTCCCTATTTGATCCTTCAATGTCTCCACTTCATAACAAAC10.5494505494505495No Hit
CACCGAGCAGGTTGCCGCAGTTGATTTGAATCAGCTCCGTGTGCGAAGGC10.5494505494505495No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10.5494505494505495No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CCGTTTTATTTTGTTAATTAAATTCTTTCCAAATTGGATCGCTCTGGGAT10.5494505494505495No Hit
GCTTCCCAGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTG10.5494505494505495Clontech SMART CDS Primer II A (96% over 25bp)
CTTTAAGATCATGCCCCTGGAGGATATGAATGAGTTCACCACACATATTC10.5494505494505495No Hit
TCTCAGGTGACATTTAGTGATGTGGCTATAGACTTCTCTCATGAAGAGTG10.5494505494505495No Hit
GTGCACAGTGCCTCGTGGCAGGCTTCATCTCACGCTTGGGGGGTGCTCAG10.5494505494505495No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10.5494505494505495No Hit
TCTCTCGTCTGAGCCCTGAGTACTACGACCTGGCAAGAGCCCACCTGCGT10.5494505494505495No Hit
GCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCAATTACTAATCC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
CATTTCGACTTCGATCACATATCGATAAATAATATTATTTCATCTATTTT10.5494505494505495No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGTAGCATTGGTATCAACG10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CAGTATGGCTTCCCCCCATTCTATGGGTTTGTGCACACAGAAATACACCC10.5494505494505495No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAGAAAG10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.5494505494505495Clontech Universal Primer Mix Long (100% over 23bp)
TGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGC10.5494505494505495No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCTGCGGATCCACGCCCACCACATCTCCCTGGCGCACGCGCAGATTG10.5494505494505495No Hit
GTACTACAGAGATGTTGGAGGGGTGAGGGAGCAGAAAGGGGAGGCAGGAA10.5494505494505495No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT10.5494505494505495No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAATTAATCGGCGTTTAA10.5494505494505495No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTCCG10.5494505494505495Clontech SMART CDS Primer II A (96% over 25bp)
CTGTTGCCACCTGGAAATCCGGGGTGTAGGAATAACGCGTCACGCGGCCT10.5494505494505495No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GGCCCACTTGGGAAAGGCTTCCTTCGCGGCGGCCACCGCGGCGTTGACCT10.5494505494505495No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT10.5494505494505495No Hit
GTATATGGGAAAAGAAAGAGCGAAATTACCTTACTTAAGGATAATAGGAC10.5494505494505495No Hit
CAAGTATCCACCCGTCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGA10.5494505494505495No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTGGGGTTTTAACACAGCTTCCCCCG10.5494505494505495No Hit
CAATAGGTAGTTTTTCAATCCTCACCCTCTTCCTATCCTCTACCCTCAAG10.5494505494505495No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGACCGTCGCAAGA10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CTCCTTATTGTCAAAAAATACTCCGTTTCATGTAATATATAGGGTATTTG10.5494505494505495No Hit
CTACCTCCCCGGGGCGGGGGGGGGTTAATTTGGGGGGGGCGGGCGTGCCG10.5494505494505495No Hit
CCCCATACACAGACTAACGACATCTTATTGGTGTCAACTTCCTAGTAGCT10.5494505494505495No Hit
TGCCTGTGCTGGGCAGTGTGTCCAGCCTGCCGAGGTCACGCTCTGGTGCG10.5494505494505495No Hit
GCACACATCACAAAGCAGTTTCTGAGAATGCTACTTTCTAGTATTTATGT10.5494505494505495No Hit
TCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCA10.5494505494505495No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTCG10.5494505494505495No Hit
AGGTGAAGGAGTCGGTGAGGGCCTGCCTCAGGACCTGCTTTCCCTCCGAG10.5494505494505495No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.5494505494505495Clontech SMART CDS Primer II A (96% over 26bp)
CCCCTATGAGGTGTCCTACCGCATTGGGGAGGACGGCTCCATCTGCGTCT10.5494505494505495No Hit
ACGCAGAGTACATGGGAAGCAGTAGTATCAACGCAGAGTACATGGGAAGC10.5494505494505495Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
ATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATG10.5494505494505495No Hit
GTACGGTCCTGCTAGTAGAGGAATATGTCGAGTTTCTCTAGGGCGCCCCA10.5494505494505495No Hit
GTAACCAGAATAAATCCTTTAACCTCTCTACTTCAGTCTGCTCATCTATG10.5494505494505495No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG10.5494505494505495No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10.5494505494505495No Hit
ACCCATCTCTCCTTAAGTGGGGATCGCCCTCACTGCCATGTGTCAGCCCC10.5494505494505495No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTGGTGCCCACCTTCCAGCTGACCCTCGTGCTGCGCCTGGACTCACGA10.5494505494505495No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATA10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GGGCCGGACTGCGAGTCTCTTTGCGGTGCTACACTAGAGCAGAGTACGAG10.5494505494505495No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.5494505494505495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.5494505494505495Clontech Universal Primer Mix Long (96% over 25bp)
CTTTAATGGCAGCATTTGGGTCCACTGCCCAAGCCACTTTTAACCCAACC10.5494505494505495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.5494505494505495No Hit
GAGCCATTGCGCCCGGCATGTAGTAAGTTTTGAAATCAGGGAGTGGGGGG10.5494505494505495No Hit
TTGTCATATCCTTTACAAACTTAGTTGAAGAGTTTGTACACAATGTGTGC10.5494505494505495No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.5494505494505495Clontech Universal Primer Mix Long (96% over 26bp)
GTCGACTGCAACCCTTATCATTTCGAATCCCGGCACGAGACGGTTGAGTG10.5494505494505495No Hit
CTCCAGTGCACTTGTAACTGACTTGGACACGGAATACTAAGAACTCACTT10.5494505494505495No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC10.5494505494505495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)