FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA544.526404023470243Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT282.347024308466052Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG181.5088013411567478Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151.2573344509639564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC141.173512154233026Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT141.173512154233026Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110.9220452640402346Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA110.9220452640402346Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG110.9220452640402346Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100.8382229673093042Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC80.6705783738474435Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA70.586756077116513Clontech Universal Primer Mix Long (95% over 21bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC70.586756077116513Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA70.586756077116513Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.5029337803855826Clontech Universal Primer Mix Long (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC50.4191114836546521Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.4191114836546521Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.4191114836546521Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAAGCGACAGACATCTGGAGGACCTGTTGATGCTAGTTCAGAGTATC40.3352891869237217No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA40.3352891869237217Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT40.3352891869237217Clontech SMART CDS Primer II A (100% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3352891869237217Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.3352891869237217Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT40.3352891869237217Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40.3352891869237217Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA40.3352891869237217No Hit
GTGACTGGAGACCCAAGATGAGAAGCGGTTATGTTCTTAGCAGCCTCCCT30.2514668901927913No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.2514668901927913Clontech Universal Primer Mix Long (95% over 22bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT30.2514668901927913Clontech Universal Primer Mix Long (96% over 26bp)
GGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGT30.2514668901927913No Hit
ACACGACGTTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGGAAG30.2514668901927913No Hit
ACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAG30.2514668901927913No Hit
CACCAACACAGTCCCATTGCGTTCACCACAAAAGCCATGAGCCCACTGTA30.2514668901927913No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.2514668901927913Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA30.2514668901927913Clontech SMART CDS Primer II A (96% over 26bp)
ACAATGCACTCTTCACCTTCAAAGCTCTTTCCCCAGCATTAACGAATGAA30.2514668901927913No Hit
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA30.2514668901927913No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2514668901927913Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.2514668901927913Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2514668901927913No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.2514668901927913Clontech Universal Primer Mix Long (96% over 26bp)
AGCCAGCCAACTCAAGAGACAAAGAAAAACTTTGAAGTAGTGGATCTGGT30.2514668901927913No Hit
GAGAAGGCTCATTTCTGAACTTTGGTCTCTCTCATATTCCTCATGTCTTC30.2514668901927913No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.2514668901927913Clontech Universal Primer Mix Long (96% over 26bp)
GAGACTGCTCTTGTCAAGGGCACCAATGAGACTCGCCTTCCCTGACCTAC30.2514668901927913No Hit
GAAAATTGTCTATTCATGTCCTTTGCCCACTTTTTAATGGGATTGTTTGT30.2514668901927913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2514668901927913No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.2514668901927913Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2514668901927913Clontech SMART CDS Primer II A (96% over 26bp)
TTCTTGTCCTTGCTGCTCCTGAGTAAAGTTTTGTTGAGATGATTCAGAAG20.16764459346186086No Hit
GAGTACACGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
GGTTGAGGTGAGCTGAGATGGCGCTGCCTAACTCCAGCCTGGGCAACAGA20.16764459346186086No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACTCAGAGTACATGGGAATG20.16764459346186086Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGACTGCACTCCAGCCTGGGTGACAAGGCAAGACTCTGTCACAAAAAAAA20.16764459346186086No Hit
GTACAGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGTATC20.16764459346186086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACCTACGTCGGCATGGTGCGGCACAACATCGAGACCATCGTGGAGGCT20.16764459346186086No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATG20.16764459346186086Clontech Universal Primer Mix Long (95% over 21bp)
GCTTTCTTAATACAGCCGGACACGGTGGCTCACTCCTGTAATCCCAGCAC20.16764459346186086No Hit
TCATGGCGCCCGTCGGGGCCGGCGTGGACGTCGCGGCCGTGATGCCCGCG20.16764459346186086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAT20.16764459346186086No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.16764459346186086Clontech Universal Primer Mix Long (96% over 25bp)
CTCCCAGCACCGAGCTTCCTCCAAACCAGGAAAAGAGAAAGAGGAAAAAT20.16764459346186086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT20.16764459346186086No Hit
GTTGTATCCCAAGCATCTTTTGTATTTGTCTTTATACTTTGCTGAATTCT20.16764459346186086No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT20.16764459346186086No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20.16764459346186086No Hit
ACGTAAAATTTGTCATCTTAACCTTTTTTTGTTTGTTTGGTTTTGGTTTT20.16764459346186086No Hit
GTACATGGGGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTC20.16764459346186086Illumina Single End PCR Primer 1 (97% over 40bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA20.16764459346186086Clontech Universal Primer Mix Long (95% over 21bp)
GCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA20.16764459346186086No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG20.16764459346186086Clontech Universal Primer Mix Long (95% over 22bp)
GGCTAGCCGTGAGACAGATGCTTTAATCAAAGAAATTTTGGCAGAAAACA20.16764459346186086No Hit
GCAGAGTACATGGGGCTGCTATCTTTGAGACAGAAAGATGCAGGACAGCA20.16764459346186086No Hit
GTGTTAAGCTACACTCTGCCCATGTACTCTGCGTTGATACCTGTCTCTTA20.16764459346186086No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
GGTGAATGGGAAGCACGACATGAATTTGAACGCGATTTGAGGTCAGCGCT20.16764459346186086No Hit
GTAGAGGAATCTCGTATGCCGTCTTCTGCTTGCCCATGTACCTGTCTCTT20.16764459346186086Illumina PCR Primer Index 3 (96% over 28bp)
GCCGTGTTATCCTTAGCACTACGCTTGACCGAATCACCAGCGGCAGAACT20.16764459346186086No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT20.16764459346186086No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC20.16764459346186086No Hit
GTACATGGGTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGT20.16764459346186086No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
CTTCTAGCCACTCTAGCCTCTGATAATCACCATTCTACTCTCTACTTCTA20.16764459346186086No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
CCAAACATTTGCTTGAGCTTAAAAAGCTCCCTCTCCAGCTCTTGCTGATA20.16764459346186086No Hit
GGTTTGATAAGCTCGAAGACGCGCATGTTGTAAGTTACCAATCGGACGAT20.16764459346186086No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGCTGTCTCTTATACACATCT20.16764459346186086Clontech Universal Primer Mix Long (95% over 22bp)
CTCTCAACCCGATTCAAAAATCCTTAGATAATAGCCCCGTCGCTGCTAAG20.16764459346186086No Hit
GGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT20.16764459346186086No Hit
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.16764459346186086Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA20.16764459346186086No Hit
CACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCCCTACACGACGCTCGTCCCAAGTTCTGCAGGTACCAGAGATCG20.16764459346186086Illumina Single End PCR Primer 1 (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTAT20.16764459346186086No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.16764459346186086Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCTGT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
TGCCGAGCCCCTCGCCATACCAGGCGCCCTCCAGCCAGGCGGCGTCGGCG20.16764459346186086No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC20.16764459346186086No Hit
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA20.16764459346186086No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG20.16764459346186086No Hit
GCGTGGAAGTGCGTGTTTGTAGCAGCTCGGGCCTCATCTCAGCGCTCGGA20.16764459346186086No Hit
GAGAAAGAGAGAGTGAGAGCACGCAGAAAAATGGTAAAAGAGAAGAGAAT20.16764459346186086No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT20.16764459346186086Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGATACCACT20.16764459346186086Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAA20.16764459346186086No Hit
GCTCCTTCCTCATCCCTCGAAGCAGCGCCGCGGCGAAGCCGCGTCGTCGG20.16764459346186086No Hit
CTTTTAATACTTTGGGGTGAGCCCCACAGGAATAAAAAACACTGGGAAGG20.16764459346186086No Hit
GCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.16764459346186086No Hit
GATAAATGCACGCATCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGT20.16764459346186086No Hit
TCCCTATGTTGGGCTAGCGGCGGATAGCAAGATTTCACTAGCAGATTATG20.16764459346186086No Hit
ATGTGTATGACACCTCAGGGGTGTATACCGACCCCAATGTCGAGATTGAC20.16764459346186086No Hit
GGATTGGGTCGCACCCATTGAAGATGAGCAGCAGATTATCCAGCGTGATG20.16764459346186086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20.16764459346186086Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGCTGTC20.16764459346186086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGC20.16764459346186086No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTACT20.16764459346186086Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.16764459346186086Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.16764459346186086No Hit
GGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAAGAAGCGGACCTTCCG20.16764459346186086No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT20.16764459346186086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)