FastQCFastQC Report
Tue 10 May 2022
EGAF00003673790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27303
Sequences flagged as poor quality0
Sequence length140
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA3841.4064388528733107No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT2811.0291909313994798No Hit
GTACATGGGAACCCCCACCGGACCTACAACCCGCAGTCCCACATCATCTC580.21243086840273964No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG520.19045526132659413No Hit
GTCTATGAGTTCTTCAAGTACTTTCTCACCAAGCGCCAGCTGGAAAATCG520.19045526132659413No Hit
GTACATGGGTGGCCGTCAGCATCCCGATCATCAAGGCGCTGCGCGAAGGC450.16481705307109107No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT440.1611544518917335No Hit
CTCATAGACAGACCAAGAAATGGCGGTGGAGGGCATCTGGTAGATGACAC420.15382924953301835No Hit
GTACATGGGCATCGGCACTGCCATCGGGCAGATATTCTTTCAGGATAACT420.15382924953301835No Hit
GTACATGGAACCGCCGTCGCGCCATCCGCGATTTCGACAGAACCGCGGTC410.15016664835366078No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG390.1428414459949456No Hit
CTCTAAGGCATTGGGGAGCAGAGGGCCCATAGGCAGCACCCTGCAAAAAA380.13917884481558804No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT370.13551624363623047No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC370.13551624363623047No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG350.1281910412775153No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG330.12086583891880014No Hit
CCTCTGGGATCTAAGGATCCAGGGAGGAGGCTGCCACAGTGAAGCAGAAA330.12086583891880014No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT320.11720323773944255No Hit
GTACATGGGAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTC310.11354063656008498No Hit
TATCAACGCAGAGTACATGGGAACCCCCACCGGACCTACAACCCGCAGTC310.11354063656008498No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA300.1098780353807274No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT300.1098780353807274No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT300.1098780353807274Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTAGTATGGAGCTCGATTTTCCAGCTGGCGCTTGGTGAGAAAGTACT300.1098780353807274No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT300.1098780353807274No Hit
GTATCAACGCAGAGTACATGGGCGGGCACGGAGAACATCTACAAGATCTA290.10621543420136982No Hit
GTACATGGGGATGACTGTCCCCCGGGGAAGACCGAGAAGATCGACGACCT280.10255283302201222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)