FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGA54.385964912280701No Hit
GATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCG32.631578947368421No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT32.631578947368421No Hit
ATTCGATTCCATTTGATGAGGATTCCATTCGAGATCATTTGATGATTGCA32.631578947368421No Hit
CTCTCCTCTCCCTTTCTTCCCTTTTCTTCCTGCCCTCGCTCACTTCCTCC32.631578947368421No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT32.631578947368421No Hit
AATTGAAAAAGGAGAGTAATGTTTAAATTCTAGCCTTTCCAAAGCTTCTA21.7543859649122806No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTTTCAGGTTTACTTTAGAATGCCCTTGGTCTAGAGGGAGGGTCCATT21.7543859649122806No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC21.7543859649122806No Hit
CCTACCAAACAGCTGGGATTACAGGAACGCCCCACCACACGTGGCTAATA21.7543859649122806No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGC21.7543859649122806No Hit
GGATCTCTCCGAGCAGCAATTCCCCATTCCTCTCCCCTATTGCTGGCTCT21.7543859649122806No Hit
TCTCAAATGTCCTCAGAATTGAACCCATTTTCAGTGTGCATGACTGGGAT21.7543859649122806No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG21.7543859649122806No Hit
TTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACT21.7543859649122806No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG10.8771929824561403No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8771929824561403Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATCACTTAATGTTCCATTGGTTCATCAGCCTTTTCTGCAGGTTCAT10.8771929824561403No Hit
TCATAAAAGATGCCCCAATAGTAGGGCTGATGTTGTGGTCAAAGTGATCC10.8771929824561403No Hit
TCCTGAAGGCTACCAACTACAATGCTCAGGAGCCCTGTAATGGCCAGACT10.8771929824561403No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG10.8771929824561403No Hit
CTCCACACGGGAACGTCGGGTCATATGCTTGAGGCTCAGTCAATGTGGCC10.8771929824561403No Hit
GTGATGACTATACCTTATTTTAACTGCACACATTCATACATTCAATCTGC10.8771929824561403No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.8771929824561403Clontech SMART CDS Primer II A (96% over 26bp)
CTCTTCCCCTTGAAAAAATAATCAATCCCGCTTACTTTATTGGTGTTACA10.8771929824561403No Hit
GCCATTCTGGATTTAAAGGAGTAATTTTAAATCCAGAATGGCAGAGCAAG10.8771929824561403No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.8771929824561403Clontech Universal Primer Mix Long (96% over 26bp)
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT10.8771929824561403No Hit
TCTGAGGTCTCTGTGGCAGGCCTGGTCAGGCTCTCCATGAGGTTAGAAGG10.8771929824561403No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG10.8771929824561403No Hit
GGACCGGCCGGCAGCGACGTGGCTCAGCACACGGCGCTCCTGCGTGGTGA10.8771929824561403No Hit
TTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCT10.8771929824561403No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.8771929824561403Clontech Universal Primer Mix Long (96% over 26bp)
CCGCTTCCGGGCTCGTCATTCCGGACACCGCTAAGGAGAAGCCGCAGGAG10.8771929824561403No Hit
ATTCCACTGTGGTCTGAGAGACAGTTTGTTATAATTTCTGTTCTTTTACA10.8771929824561403No Hit
TCGCTGGTGCCGCCATCACCGGTAAAGGCCAACGACACTTTGTTTTGTTG10.8771929824561403No Hit
GGAGGGCTCCATCTCATGTCGAAGGCCAAGGCTAAGAGTGGCTGCCTGGG10.8771929824561403No Hit
TTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAA10.8771929824561403No Hit
GTTCACCCACTAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAG10.8771929824561403No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG10.8771929824561403No Hit
GTATGGTGGTCACCTTTGCTCCAGTCAACGTTACAACGGAAGTAAAATCT10.8771929824561403No Hit
GTTGTTTGAATTTGCAGTGATCATCTCAGTTCTTATGTGTCTTCTGGTCT10.8771929824561403No Hit
GTCTGCTGTAGATAAGAAAGGAGATACTCCCTTGCATATTGCTATTCGTG10.8771929824561403No Hit
GTTCCTGGGCCTGCTGGAAAGTAACTTTTTTTTTTTTTTTTTTTTTTAGA10.8771929824561403No Hit
AGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGG10.8771929824561403No Hit
TTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAA10.8771929824561403No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTAAC10.8771929824561403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG10.8771929824561403No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.8771929824561403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTGATGGGACATATCTCAAAAAAATAAGAGCTATCTATGACAAACCC10.8771929824561403No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC10.8771929824561403No Hit
GGTTCAAGTGATCCTCCCACCTCAGCCTCCTGAGTAGCTGTTACTATAGA10.8771929824561403No Hit
GTACATGGGACTTGGGTACTCAATGAGAAGGAATGCATACTGTCATTTGC10.8771929824561403No Hit
GTTCTAGAATGTGGTACCACATAAGACCTGCGCCCCAGCACATCTCAACT10.8771929824561403No Hit
GTAGAATCAGTATATCCTGAAGGCAGTGCTGGAGGCAGTCTCATGCACTA10.8771929824561403No Hit
CGGACATGCAGAGGTGGCACTCAGAGTCTCGCGGCAGCCATTCTCCTGTC10.8771929824561403No Hit
GATCAAAAAGCGACGTCGCGATGAACGCGTGGCCGCCACAAGCCACGTAT10.8771929824561403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTCCA10.8771929824561403No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA10.8771929824561403No Hit
GTTCGACACCCTGCCCGCCACGCAGGGCTTCATCAAGGGCTGTCTCTTAT10.8771929824561403No Hit
CTTCAAAGGCTTGGCAAAGGCTCCGCAGCGCACGGTTCTCGGCACCACTA10.8771929824561403No Hit
AACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG10.8771929824561403No Hit
TTCGATGATTGCATTCAGTTCATTCGATGACGATTCCATTCAATTCCGTT10.8771929824561403No Hit
CTCAGCTGGAATTAATGTTTGGATTCTTTTGTGTGACATCAACTCATCCC10.8771929824561403No Hit
GGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACT10.8771929824561403No Hit
CCCTAAAACCTTCCAGCAAGGCCTCACATTTTCACCTATATCGAAGACTT10.8771929824561403No Hit
ACATAGGCATATGTGTTGTACTTCTTCACGAAGCTTGCAATGGCCTCATT10.8771929824561403No Hit
GTGTTCATTCAGTATGGGCTGCACCCCTACAGGGCTCTTCGGTGTCTTCT10.8771929824561403No Hit
TCTGCATTGAAAAATCTTTTCTTTTAGAGTGTTGAATGAATATTGGCCCC10.8771929824561403No Hit
CGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGC10.8771929824561403No Hit
ATCCTAGATACTCAGGAGGCTGAGGCAGGAGAATCGTTTGAATCTGGGAG10.8771929824561403No Hit
GGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGA10.8771929824561403No Hit
TTCGTGATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC10.8771929824561403No Hit
GTTTTAGGTTCCCTGGGGTGAAGAAGGTATAGGCGGTACCCTTGTTGGTG10.8771929824561403No Hit
AGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGGT10.8771929824561403No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10.8771929824561403No Hit
GCTCTGGGCTTGGCTGAAACATGACGGTATTGCTTGGTTTCAGACTTGAC10.8771929824561403No Hit
GGCACTTAGTGCCCACAACCGCACCAAGAAGCACGACCTGCTGAGGCTGG10.8771929824561403No Hit
GGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGAT10.8771929824561403No Hit
CCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCT10.8771929824561403No Hit
AGAAAGAGGAGTGAGGAAGGCAGATGGGTTAGAAAAGAGGATCCAGGAGA10.8771929824561403No Hit
GCTTCTGTCGGGCAACCAGGCCCAGCGCACTCTATTTTGCCAAAAGAAAG10.8771929824561403No Hit
GGTCTGTGTCATTGCTTGCGCCTTCGTTGCTTCGGCTAAAGGCGTAGCGC10.8771929824561403No Hit
GTTCAGAATCGGTTCCATCAGCGAAGAGTGCCAACCCTGCTGTAAGTAAG10.8771929824561403No Hit
GGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGG10.8771929824561403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)