FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCTGGTTCTTGTAATTTGATTTTACAGAAAAGGAAATGACACTTGAG35.769230769230769No Hit
GAGCAGCTCATCACAGGCAAGGAAGATGCTGCCAATAACTATGCCCGAGG35.769230769230769No Hit
CGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTG23.8461538461538463No Hit
GTGAGCTGCATTACTCTTTTTCTATGAGGTATAATTTGAGGAAATAAGTT23.8461538461538463No Hit
AGATGGAGCAGCAGAACCAGGAATACAAAATCCTGCTGGATGTGAAGACG23.8461538461538463No Hit
TATTTATTGTAGGAAGGAAGGAAAGAAGGAAGGAGAAATAATTTGCAAGG23.8461538461538463No Hit
ACGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC11.9230769230769231No Hit
CTGTAGAGAGAACACAGTGTGAATGTTTCCCCTCGTGTGAGCCCAGCCTG11.9230769230769231No Hit
GGTATAGCCATACTGGGGGTAACTGTAGCTGTAGCTGCCTGTGTTCTGGT11.9230769230769231No Hit
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAG11.9230769230769231No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
AGGCAGTGAAGGAAAAAAGTCCTTCGCTGGGGAAAAAAGATGCGAGACAG11.9230769230769231No Hit
CACCATTGAAGAATCAGCAGCCAATAACATCGTCTGGCTGCTCTCGAATC11.9230769230769231No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG11.9230769230769231No Hit
CTGGAGAGAAGCCGTTCAAATGTCCTTTCTGTAGCTACGCCTGTAGAAGA11.9230769230769231No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATG11.9230769230769231No Hit
TTATTGGTCTTGCCCCTTTCTCTAAATTTGAAGGTAGTTGGCACCTATTG11.9230769230769231No Hit
GGTTGGTGATCACAGCCAAGTGGAGTGGAGTCTGCTGCAGGTTGTTCTGG11.9230769230769231No Hit
TATGCATATGCCTGTTGGTTCATAATTGCTCTTTATATTGTGTGTTATCT11.9230769230769231No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA11.9230769230769231No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACTGGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGG11.9230769230769231No Hit
CTGGTAGTCGCCCTTAGTGTCTTGCTGGATATAATAGTACAGGGACTTGC11.9230769230769231No Hit
TTGTAAGACTTATTGAAATTTATCTTTGCAGTGTTTGTGTTAGGCCATGT11.9230769230769231No Hit
GGACAGATACTTCGTTTATATCGTAGGAAAAGACAACAAGGTTAAGTATA11.9230769230769231No Hit
CAGCTGGAGGAGTTCAAGGAGGCCTTCGAGCTGTTTGACCGAGTGGGGGA11.9230769230769231No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
TCCTCATCCCTGTGTTAGACCGGATCCGATATGTGCAGAGTCTCAAGGAA11.9230769230769231No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
TCTTAAGGCTATGGGAGGTTATTTCAGCAGCTGGGCTTGTGAGACAGTTC11.9230769230769231No Hit
ACCTTGTCTCTACCAAAGATAGAAAAATTAGCCAGGCACGGTGGTGCACG11.9230769230769231No Hit
GTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAG11.9230769230769231No Hit
ACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGA11.9230769230769231No Hit
GCTTGAAGGCCATGGTGGAGGCCTGGATCTTCTTTACTGGGGCCTGCCCC11.9230769230769231No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CTTTGTATCCCTGCCCCGAGGGCCTCATAGCCAGGTCTCCTGAGAGCAGA11.9230769230769231No Hit
GACACCATTGAGAATGTCAAAGCCAAAATTCAAGACAAGGAGGGTATCCC11.9230769230769231No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCT11.9230769230769231No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT11.9230769230769231No Hit
GTATTGATCATGCCTGATTAGGGTCCTTTTCCCCAAATTTGATGAAAGAA11.9230769230769231No Hit
ATGTATGGCTAAGTGGTGATGTGGTGACAACCTTGAATGCTAGTCCTGGA11.9230769230769231No Hit
CACATTGCTATTACGATTCAAAAGCCTCCATAAACTGTCAAACTGGCAGA11.9230769230769231No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAGTACACTGCGTTTATACCTTTCT11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)