FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGCCAATGGTGTAGTGCCCTCGGGCATAGTTATTGGCAGCATCTTCC35.769230769230769No Hit
ACACTAATGCAGCAACAATGCATTCATTATTGTTCTCATTAGTCATTAGT35.769230769230769No Hit
CACATATTCCACAAAGATGGTAATACTTTAAAAATGTTCTATGGAAAAAA23.8461538461538463No Hit
GGGGCAGATGGGGCGGCTGCCTCCCTGCCTCCTGGGTGGCCGGCCGGGGT23.8461538461538463No Hit
CTTATAACTTACGTAACTTAGATACAGCCACACCAAAATTCTTTCTCTTC23.8461538461538463No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23.8461538461538463No Hit
CTACCACTATTTCCAGTGGCGCAGGGCCCAGCGCCAGGCCGCAGAAGAAC11.9230769230769231No Hit
CAGTGCATTCAGAACAAGCCCCTGTATTTTGCTGATCGGCTGTATGACTC11.9230769230769231No Hit
GGTGTGGGCCTCCACCAGCTCGGCCACGGTGGGCGGTGGGCCAGCGTAGC11.9230769230769231No Hit
GGGCCTGCAGTGCCCCCCGCCACACACACCATGATGCCTGAAATGCGTGA11.9230769230769231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGTTGATACTGTAA11.9230769230769231No Hit
GGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11.9230769230769231Clontech SMART CDS Primer II A (100% over 26bp)
TCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC11.9230769230769231No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGA11.9230769230769231No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG11.9230769230769231No Hit
AGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGGGACCGGCTATCCGAGGCC11.9230769230769231No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG11.9230769230769231No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.9230769230769231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGTTTATACTGTCT11.9230769230769231No Hit
CTGTACATTTACTTTTGTGAAAACACTGCCTGCATTTTCTAGTACAAAAA11.9230769230769231No Hit
GGGCCTGGGCAGGAGGCTGCCCTTCAAGGAAAGAACAACCTTATTTGACC11.9230769230769231No Hit
GCAAATTGAGAGTAAATGAGTGTACACAGGAAGGAGCCACAGCTCTGCCA11.9230769230769231No Hit
CATAACGAATGTTCAAAGTTCGTGCATCACTCTGCTGTTCCCTACACATA11.9230769230769231No Hit
GCCAGAACGGTCTCCACCTCCTCCTCAGTCATCTTCTCTCCAAGGGTGGT11.9230769230769231No Hit
GCCTACTCTGACATGAGAGAAGCCAATTACATCGGCTCAGACAAATACTT11.9230769230769231No Hit
GATTTACTAGGAGTGGTGAAAAACTTGGCCTCTGGCTTTTTTGGAGTCTG11.9230769230769231No Hit
GGGCTGGGTCCAGCCTTCCCTGGGCAAGGAATCCCGGGCCAAGAAATTCC11.9230769230769231No Hit
CCATTGAATCTGGATGAAGGCCAGATGATGGATGGACCATAGCCAGGCCC11.9230769230769231No Hit
TCCCTAAAGCGAGCCGTGTAAAGCCAGTGGAATATAGTCAGATGTACAGT11.9230769230769231No Hit
GTCTTCTTCAGTTTCTCCTGATATTGAGCCTCTGTTATCGGTGTTATCTG11.9230769230769231No Hit
ATAATAGGCTCTTGTTCCTTACAATCTTCCATAGGAGGTACATGGTGACT11.9230769230769231No Hit
GTGCCGAAAGGATGTGGGCCGGCCCCAGCCGGGACGCGAGAACTTCCAGA11.9230769230769231No Hit
GACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTC11.9230769230769231No Hit
GCTCTGATCTCATGGTTGTTTGAGCTAGCTGGGTGACGGCATACTCAGGG11.9230769230769231No Hit
CCTCAATGCCTCCCTTGGGATGCAGCATCCAGGCAGAACCCAGCCGTGTC11.9230769230769231No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAATCTCCCTCTGCCTCCTTTGTATAGGCAATTTCATTAGGGCCCAC11.9230769230769231No Hit
AGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCC11.9230769230769231No Hit
CACGGAAGTGCAGAGTAGTCATCTTACTCAGCTCAGGCTGACTAACAAAA11.9230769230769231No Hit
CCCCAGGAGTGGGCCATGGAGGGCCCCCGCGACGGGCTGAAGAAGGAGCG11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)