FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35.88235294117647Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGT23.9215686274509802No Hit
GGTTAATAGTCCTACACCGTATTCGGATCAGATGCGCCTGTTGGCGCAGT23.9215686274509802No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
ACTATCGGGTGGAGCGCCTGCGAGACCGGCTGGCGCCCAACCAGGTGGGC23.9215686274509802No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23.9215686274509802Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGAAGCAGTGGTATCAACGCAGGGTACATGGAAGCCTGTCTCTT11.9607843137254901Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11.9607843137254901Clontech Universal Primer Mix Long (96% over 25bp)
TCCTCAAGCAGCAGATGTTCATGCACATCAACGAAGCGCTGGAGCGAGGG11.9607843137254901No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCAGCACCAGCAGCGCGATGCCCCAGCCTGGCACCCCAGCCCCAGACT11.9607843137254901No Hit
GGGAAGCAGTGGTATCAACGCAGAGAACATGGGAAGCAGTGGTATCAACG11.9607843137254901Clontech Universal Primer Mix Long (96% over 25bp)
ATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTA11.9607843137254901No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT11.9607843137254901Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCCACGCAGAGTACATG11.9607843137254901Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCAATGAAGGCCTAGGTAAATTGTTAGCTTGTTTCTATGTTTAGATAT11.9607843137254901No Hit
GGATGGAGGCCGAAGGCCAGAGAGCGTCCTCCCTGTCCCATGGCCTAAAT11.9607843137254901No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCCAGCGCCTGCACCAGCCCCTTAGCCACCAGTGCCTCCAGAGCCTTCCA11.9607843137254901No Hit
GTGGTGGTCGACATCGCGATCGACCAGGGCGGCTGCTTCGAGACCAGCCA11.9607843137254901No Hit
TGTCTGTGGCTATGACTTTGGCACCTTCTCTTGCAAAAGCTAAGGCAGCT11.9607843137254901No Hit
GGGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAG11.9607843137254901No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATG11.9607843137254901No Hit
AGGGAGATGTTACAGCCCAGATAGCTCTTCAGCCTGCACTGAAGTTCAAT11.9607843137254901No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTACCCAG11.9607843137254901No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGTGAAGGTTTCGAGTACGTCCTTCTTGGGGCAGATGTCTGAGATAAAC11.9607843137254901No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.9607843137254901No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC11.9607843137254901No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG11.9607843137254901Clontech Universal Primer Mix Long (96% over 27bp)
CATCCCCACCTTCGGGAGGAGCTTCACTCTGGCTTCTTCTGAGACTGGTG11.9607843137254901No Hit
CTACCCCGGCCAGCTTTCCGGTGGCCAGCAGCAGCGGGTAGCCCTGGCCC11.9607843137254901No Hit
GTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATACCACT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GGTAGGAGGAGGCGAAGGTGTTGTCGACGACGAGCCGCGCGCCCGCCTCG11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAAGACCGATCGCGGTTG11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)