FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length140
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47.8431372549019605Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC47.8431372549019605Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA47.8431372549019605Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35.88235294117647Clontech Universal Primer Mix Long (96% over 26bp)
TCACAAGCCTCGACTCCTCCGCAGCCAAAGCCTCACCCGAAGGCGGATTA23.9215686274509802No Hit
AGGGACACCAGCGCAGCCGCCTGCAGCTCGGGTGTGTCTGTGGTTATCTG23.9215686274509802No Hit
GCATAATCACTTGTTCCTTAAGTAGGGACCTGTATGAATGGCTCCACGAG23.9215686274509802No Hit
CACCTGGGCCTGTGCAAATCCCGGCAGCCAGAGCCAGAGCAGGAGCCAGG11.9607843137254901No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CTCGACGGCCCTGAGCTCAAGTTCAACCCTGGCCGTATTGACCCGACCAA11.9607843137254901No Hit
CATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCC11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT11.9607843137254901Clontech Universal Primer Mix Long (96% over 25bp)
CACCATGACACCGGGCACCCAGTCTCCTTTCTTCCTGCTGCTGCTCCTCA11.9607843137254901No Hit
CTGGGGATATTCCCACAAGGATGAGGTGATTAAGGAAGTCCAGGAGTTTT11.9607843137254901No Hit
GCCTTGGACACCAACAGATGCAGCCGTCAGCTTCTCCTTAAACTTGTCAA11.9607843137254901No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGT11.9607843137254901No Hit
GGTATCAACGCAGAGTACATGGGAAACCGAGTTCTGGAGAACGCCATCAG11.9607843137254901No Hit
GCCGAGCGCCATGGTCGAAGTGCGCGCGACCGCGCCGGGCATGTTGGCGA11.9607843137254901No Hit
ATACTTGATAACTTGTTGATAACAATATGAATAAAATTTATTCACCTGCT11.9607843137254901No Hit
GCTTCCATGTACCCTGCGTTGATACCACTGCTTCCAAGTACCTGTCTCTT11.9607843137254901Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCTATGTAGGCCTGACTGAGGGTCCCAGCCCTGGAGACTTCCGCTACT11.9607843137254901No Hit
CCCGGCGACCACGTCGTGATCCCCGACGACGCCTACGGCGGCACCTTCCG11.9607843137254901No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.9607843137254901No Hit
CCCCTCGCTCCAGCGCTTCGTTGATGTGCATGAACATCTGCTGCTTGAGG11.9607843137254901No Hit
TCTATGGACTGTGGCTCCGCGGAGGAAGTCACAGATCTCATAGTAGGCAA11.9607843137254901No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG11.9607843137254901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCT11.9607843137254901Clontech Universal Primer Mix Long (96% over 27bp)
TTCACCGCCAGCGCTTCGACCCGCTCGCCGCGCGGCAGCTGCACCTGGCA11.9607843137254901No Hit
GCCTGAGATTGGGGAGGCCCTGAAGGAGGACGTGGGACCAGGCAAGGCGG11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)