FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003673844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTGGGCATGTAACGGGTTTCGGGATCGGCGCCGGCGTTGCCGACGA519.230769230769234No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT311.538461538461538Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG27.6923076923076925Clontech Universal Primer Mix Long (96% over 26bp)
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA27.6923076923076925No Hit
GATTAGAACTTTAAAATATCTATAATCATCATGCTAAGAATTTTAATGGA13.8461538461538463No Hit
GGCCGCGCTTGTCGATGTCGGTGAGGCTGCCGGCGCCGCCGAGGAAGCCG13.8461538461538463No Hit
CGAGCACTGTTTGTGGGAGGTATGGGTTAAGACAGATTCAGGGCTGTCTC13.8461538461538463No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGGGG13.8461538461538463No Hit
ATAGAACACGAATGGGAAATACACAAATGCTAAACTAAAGAAGATATTGG13.8461538461538463No Hit
CTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACG13.8461538461538463No Hit
TTTGTCTGGTGCCCATGCAGCCAAATACCTTATCAAGCCGACCGATTGTG13.8461538461538463No Hit
GTATAGTACCGAACGCCAATTGGTAGAGAATGAGCCAAGTAAAATTTTGC13.8461538461538463No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG13.8461538461538463No Hit
TTTGTCTGGTGCCCATGCATCCACATACCTTATCAAGCCGACCGATTGTG13.8461538461538463No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG13.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GACGATGGCATCACGCTGGTCAACATCGGCGTCGGCCCCTCCAACGCCAA13.8461538461538463No Hit
ACTGGCGGGTGTCGTCGACCATCTCCGGATTGCCGCAGATGAGGATGCGC13.8461538461538463No Hit
GAAATTGATAGCAATCGCTCACCCAAACGTTTGATTAATGCGTTTCCTTG13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)