FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003673925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003673925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCT43.4482758620689653No Hit
GCTACGAGCTGCCTGACGGCCAGGTCATCACCATTGGCAATGAGCGGTTC32.586206896551724No Hit
ACTTACATTTGATTAATTTGTGGCATTCATGTGTCTAATTTTCTACACAC21.7241379310344827No Hit
CCATCACCATCATCACCATCACCACCATCATAACCATCACCTTCATCACC21.7241379310344827No Hit
TCCCAAAGTGCTTGGATTACAGGCGTGAGCCACCACACACCTGGCCTGAA21.7241379310344827No Hit
ATCGCTCCCTGCCGCCTGGCCATAAAGGACGCCGGCATCAGCGTGGGCGA21.7241379310344827No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG21.7241379310344827No Hit
CCTCACAGCCTTCCTGGGATACAAGGCTGGCATGACTCACATCGTGCGGG21.7241379310344827No Hit
GGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGC21.7241379310344827No Hit
CATCGAATGGACTCGAATGGAATCATCATAGAATGGAATTGAAAGGAATC21.7241379310344827No Hit
GTTACAAATAATCTTAGGTAGGTGTTGTAAACATCAGAATACACTTCAAA21.7241379310344827No Hit
GAATGGAATCAACACGATTGTAATCGAATTGAATGGAATGGAAGTAACCC21.7241379310344827No Hit
GTTTCCACAGGAAGAGTCTGTCTGTTCCATGCGCTCACAGTCGAGGCTGA21.7241379310344827No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG21.7241379310344827No Hit
GGCATGAAGCGGCTTAAAGTGCAGCTCAAACGTTCGAAGAATGACAGCAA21.7241379310344827No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA10.8620689655172413No Hit
GCCAAGACAATCCTAAGCAAAAAGAACAAAGCTGAGGCATCACGCTACCC10.8620689655172413No Hit
CACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGAT10.8620689655172413No Hit
ATCCGCGACGCGCGGGGCATGGCGCTCAAGTTACTCGACGTACCGGGCAA10.8620689655172413No Hit
ACCACCATCACGGCCGGGTTGCCGAGCATCAGCACGTTCTGTCCCGCGGG10.8620689655172413No Hit
TGGTTCCATTCGATTCCATTCGATGATGATTCTATTCGAGTCAATTTGAT10.8620689655172413No Hit
TGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGC10.8620689655172413No Hit
CCCAAGAGACAGAATGGACCCGTTTTCCCTCCCCCACCATCCCCCATCTT10.8620689655172413No Hit
CTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTC10.8620689655172413No Hit
ACTCAGTTTTGGTAGAATTTCCAGGTGGATATTTAGCGCCGTTTGAGGCC10.8620689655172413No Hit
CACCGAGCATGCCGAGCCTTTGCAGCAGATGCCGCAGCAGCAACTGCGCC10.8620689655172413No Hit
CAAATAGACGCAATAAAAAATGATAAAGGGGATATCACCACTGATCCCAC10.8620689655172413No Hit
GCATTTTTTCATGTGTCTTTTGGCTGCATAGATGTCTTCTTTTGAGAAGT10.8620689655172413No Hit
AAATGTTACCACCTCCTGAAAGGACACACACCAGCACCCCAGGAATCCCA10.8620689655172413No Hit
GTCTCCTGTTACTCAGTGGGCCTCTGTTGACACTGCCACTGTCCCCACCC10.8620689655172413No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC10.8620689655172413No Hit
GCCCCTTGATGAGGCCCACCCCACGGGTGGTTGTACCAACCCTTACGGCA10.8620689655172413No Hit
CCCAAGAGACAGAATGGACCCGTTTTCCCTCCCCCACCTTCCCCCATCTT10.8620689655172413No Hit
AAAGTGCTGTTGTGCTCTATTTCTCAAAACTCCAAATTTGATTTTTATCT10.8620689655172413No Hit
GTTCTTCCAAGGCTAGGATTTTTACTTTCACAATTCTATACTCTACGTCT10.8620689655172413No Hit
GTCACAGTATAGGATGGTCTGGACTACGCCGGACACCACAGCAATGAGGT10.8620689655172413No Hit
CCTCCCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGA10.8620689655172413No Hit
ATATTGCCTCGTAGTTTCTGTAGGTCTGGACTCTGGTTATAGTTACCTGG10.8620689655172413No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAATTGCCTCG10.8620689655172413No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA10.8620689655172413No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT10.8620689655172413No Hit
GAGGAGTGGGGTGGCCACTCCCCCCGGAGTCCCAGGGGATGGGACCAGGA10.8620689655172413No Hit
GAATACTTGACAGCCAACCCTCCTCCTTCGCATCTGCCCAAGGAAATCAA10.8620689655172413No Hit
TCCTTGAACATTTATTTTCTGATAAAAGAAGGTATTTACTTATTTGAAAT10.8620689655172413No Hit
TATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAA10.8620689655172413No Hit
AATGGGTACTCTGCTGGAAAGTTGATTTCGATTCTGAAGGCTCCCTTATC10.8620689655172413No Hit
GCGCGAAGGTCCAGCGGAAGCCGTCGGCCGTGGCTTCCACTGCCGCGGCG10.8620689655172413No Hit
CTCCTGTTGATAAATATTAGGGCAGCTTGGAGTTTTTTGCTGTTGTATAC10.8620689655172413No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT10.8620689655172413No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGGAGAGTACAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACGGCCCGAGGTTCAGCTCGCCCGTGGAAGTACCGGATGCTGCCTTTG10.8620689655172413No Hit
CTTAACTCACTTAAGGCTATCATTCTTTCTCATGTTTTTTTTTTTTAATA10.8620689655172413No Hit
GGTTTTATCAGTTTCTTCTATGGAGGAGTGAGGCATGAATGAGACTGGAG10.8620689655172413No Hit
GAAAAGACAAGATTTAGCTGGGGTATATGGCCTCATTTTCTACCTGGTTA10.8620689655172413No Hit
CATTCCTACACACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAA10.8620689655172413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTT10.8620689655172413No Hit
GGCGTGTAGGCGCGCGCCTGTAATCCCAGCTACTTGGGAGGCTGGGGCCT10.8620689655172413No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA10.8620689655172413No Hit
GTGTTCATTAAGCCCCTCTAAGGCAGAACTTGTCCTCCCAGCCCCAGGAG10.8620689655172413No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10.8620689655172413No Hit
GCCTGTCATCTGTGCTACTCAGATGCTGGAGAGCATGATCAAGAAGCCCC10.8620689655172413No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA10.8620689655172413No Hit
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC10.8620689655172413No Hit
CTACACATCTGCCTCTGGTGATGAGATGGTTTCTCTCAAGGACTACTGCA10.8620689655172413No Hit
CGTGGAGGAGGATGAGAGTTTCTGTGGAGCACCTTTTAATGTGAAAATGT10.8620689655172413No Hit
CCTTGAGATAGACTTCAGAAGCAGATCCTGATGTGAAGATTCAAATACAA10.8620689655172413No Hit
GACACTCTGAGACTGGGCCAGATTCCCCTAGCACATACACACCTTCCTAG10.8620689655172413No Hit
CCAAAAAAAAGTCCAGGACCAGATGGATTCACAGCCAAATCCTACCAGAG10.8620689655172413No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT10.8620689655172413No Hit
ATCTTGGTCTTCATTTTCTCTCAGTTCTCTTTCTTTGCCCTCCCAATAGT10.8620689655172413No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG10.8620689655172413No Hit
GTCGAGCGCGGGCACGGTGAAGCTCTCGTCGCTCTTCACGCGGCGGTGCG10.8620689655172413No Hit
GGGTATAACAATTATGAACAGATGAACCATATTCATAAAGAAATACATCA10.8620689655172413No Hit
GGCGCCAGATGTCGCAGCAGTATTCGCTGTCGAAAGCCCGGTGACGGAAC10.8620689655172413No Hit
ACATTTCACCATATGGCAGCTGTCTGAAGGCTGTTTTGCAGGAGGCTGGT10.8620689655172413No Hit
AGACAGAGTTTCACTCTGTCACCCAGGCTTGAGTGCAGTGGCATGATCTT10.8620689655172413No Hit
CCCAGAGGCTGTTGTAATACTGCGTTTGGAGACTGCAGAAGTGCCTCTTG10.8620689655172413No Hit
GTTTTTCATACTAAACCAACAGCTTGTGTGTAACTTTTCTAATATCTTCC10.8620689655172413No Hit
TCCAAGATGTATCCTATCAATTATAACAAAGGTATTTACAAATAGGCTAA10.8620689655172413No Hit
AACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGG10.8620689655172413No Hit
GACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCT10.8620689655172413No Hit
GGCATTGCTCCAGGCACTGGGGATCTAGTGGAGGAATTGGACTAATGAGT10.8620689655172413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAG10.8620689655172413No Hit
CCTCTTGGTAGCCATTGCATTGAATGTGCTTTGTCTTCTGGGTCATACTG10.8620689655172413No Hit
GTGCCGGGCACCCATGTCTCGGTAGCACTGGGTGACAGCGCCTGCGGTGG10.8620689655172413No Hit
GGCCTGCACGGCGGCGGCCCGCTGGCCGAGCACGCAGAGGCCTACCTGAT10.8620689655172413No Hit
CCCAGACCTTCCTTGCCTACAGAACACATCTTAACTCCTTCAAATGTCAT10.8620689655172413No Hit
TCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCA10.8620689655172413No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG10.8620689655172413No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA10.8620689655172413No Hit
GTTTCTGCAGAATTGGAAGCCTCTCATGCACAATAAACCTTAACCACTCA10.8620689655172413No Hit
CTACAGGCGCGCGCCACCACACCCAGCTAATTTTTGTATTTTCAGTAGAG10.8620689655172413No Hit
CATCTTTGGGTTCCACAATGCTCACGTGGTCAGGCAGGGGCTTCTTAGGG10.8620689655172413No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
AGACAGGGAAGCAGATTATGCAAACAGAAGGAAGGGAGGAGAGATTAGGA10.8620689655172413No Hit
CCAGTGCGGCGAGCAGTCCGAGGTGAAGACGATCGACCGTCCGGCGCCGT10.8620689655172413No Hit
TAACAATTGTGTGTCTGTGTGCATGTGTGTGTACACATATATACATGCAT10.8620689655172413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)