FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAAAACAGCAGCATGCCCAGGCTCAAGCCGATCACGATAGTTGACAT42.898550724637681No Hit
GGTATTATTAGACTGAAATATTAACTTTAATAACATAAGAAGCATATTAT32.1739130434782608No Hit
GTGTTCTGCATCGAGCAGGGCATCTTCGCGCGCGACGACCGCGACGCCAT32.1739130434782608No Hit
TTCATATGTTGAACACATCTTTGCTTTCCTTGAATAAATACTACTTGGTC32.1739130434782608No Hit
TATTTTCTCCCATTTTGTGAGTTGTCTTTTAACTTTGTTTATTATTTTCT32.1739130434782608No Hit
ACCTTTAACAGTGAATTTGGATTTCCAAAGAAACAACAAAACTGTCTTCA32.1739130434782608No Hit
GTTTGTCAGTGGAAGCGCGAGAAAATCGTCTGCCAGAATCCAACATGGTT32.1739130434782608No Hit
GCATCAAACAGTGCCATGATCGTCTCATTACGATTCTCCTTCGAAACCAA21.4492753623188406No Hit
CTTCCGCGGACGCGGCCGGCTCATGCGCGGCCAACGGCAGGTGATGCCAT21.4492753623188406No Hit
CTTTTGGAGGAGTGCTTAGGGTTTTCTAGGTATAAGATTATGTCATCTGT21.4492753623188406No Hit
ATTTTAGGCAGGATATAGTGGCTCACACCTGTAATCCCAGCACTTAGGGA21.4492753623188406No Hit
GCCGTGTTCAGTGCTCAGGACCACAGGTCCCAGGACAACATGAAAACAAT21.4492753623188406No Hit
GTCATCGTGTCGGCGACATCATGGGTGATGATCAGAGCCGTCATATTGGT21.4492753623188406No Hit
ATTTATAATAAATTTCATAGAGTGCTTATCCAAAAAGTAAGCTCATCATT21.4492753623188406No Hit
GTCAAGGACGAGTTGGCTACGTCAGAGCGTCCGATGTCCGGTCTCATGCT21.4492753623188406No Hit
TCCTTGGTTTGCTCTCCTAAACAGGTGTTTTTCATACTTTAAATGGCCAG21.4492753623188406No Hit
GTTTGGTTTTTTGTCCTTGCGACAGTTTGCTGAGAATGATGGTTTCCAGC21.4492753623188406No Hit
CATGTGCACACACTTGCAAAGAAATTATATTTGGAAGAATATTTTAACTA21.4492753623188406No Hit
AAAGTATCCATCAGTGTGACAAGAGGTCACCTTCGAACTTGCATGAACTC21.4492753623188406No Hit
GTCGACCAGCCTCGTCGGCCCGATCCTCGCCCGTGTCGTCTCGCCTGCGC21.4492753623188406No Hit
GCCACCATCTCACTGTTCAGAAGAACCTCAAGAGCAACTTCGTTGGTTGC21.4492753623188406No Hit
TGTGTCTGACCATGTTGTTGCAAGCCACCTAAGACGTGGCTGGACCCAGC10.7246376811594203No Hit
CTTGAAGGCCGCATCCAGCTTGTCATTGCCCAGCACATCGCGCAGTGCCT10.7246376811594203No Hit
CCCCCTCAGCCTTTTTCCAAGAAGGTGGGCTTTGGGGTGTGGATTCCACC10.7246376811594203No Hit
ATTCATGGACCTCCAACTCCAGGACCAGCACGTACTCGTCACCGGCGGCA10.7246376811594203No Hit
GTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCGGCGGGCCGCC10.7246376811594203No Hit
GTTTCACCATGTAGGCCAGGCTGGTCTCAACCTCCTGACCTCAAGTGATC10.7246376811594203No Hit
TCCCAGCACTTTGGGAGGCTAAGGTAGGTGGATCACGAGGTCAGGAGTTC10.7246376811594203No Hit
GTGTTAATAGGATGAGTACCAATTCTTTGAATGTCTGATAGAATTCAGGT10.7246376811594203No Hit
GGATAACGCTGAACCGTTACCCCGTAGCTTCAACAAGCCAGCTTCCTGCA10.7246376811594203No Hit
CTCCCTATACTGCCAGAACACTGGGGTCAAAAATGGCACCACATGAGATC10.7246376811594203No Hit
ATGCTCATGATAGAAATCAAGCGCAAACATTGCGCCATGGGACAGAATCC10.7246376811594203No Hit
AAATTGAATTCAGATAAGATTTCCATTTGTATCAACACAAATTAAAGTAT10.7246376811594203No Hit
ATCTAACTTTCATACTGTACTGCATGGTCATTAGTTTCCTATTCACAAGC10.7246376811594203No Hit
CAGCCATAGTAACGACCACGCCTATCCTCGCCGACTTGACGCGTCAGGTT10.7246376811594203No Hit
GCCACGTAGGGTGAGGCGTTTCCCGATGGCCAATGCGAGATTTCGGGGGG10.7246376811594203No Hit
ACTTTGTTCTATAGAAGGAGTCTCAGATAAGACTTTTTTAAAGCTGAGCC10.7246376811594203No Hit
ATGTTTAGCTTTTAAGTCCACATGGAATTTATTTTTGTGACTGGTTTTTG10.7246376811594203No Hit
AATTAGATGACGAGGCAATTGGCTACCTTAAGAGAGTCATAGTTACTCCC10.7246376811594203No Hit
CCTAAAGTGCTGGAATTACAGGTGTGAGCCACTGCACCCGGCCAGGACAG10.7246376811594203No Hit
TCCTGAACCAAATAGACGAAGTTGACTATGGTACAACGACACTATATCAT10.7246376811594203No Hit
CTGACAAACTGGAGCGGCTTTTCTGGGCACGATCATAAGGATTTTCGGCC10.7246376811594203No Hit
ACCCAAGACTGTCTCACTACTTGGATGAGATTATTGGAGAAGGTTGGCAC10.7246376811594203No Hit
GCGATAGCCATGCGGTGCTCAAACTCCAGTACGGGGCAATTGCTGGTTGC10.7246376811594203No Hit
AACTAGGATGGACCAAGGGTTCGCGTCTCACCAGCGAGTTCGACGTCACG10.7246376811594203No Hit
ATATTTATATGGAATTTCTTAGACTAAGAATTTCTGGGTACATCAGCAGT10.7246376811594203No Hit
GAGTGGCTGGGGGTGGAAGCCAGGAGAATGCTCTGAAGACTCTCATCATG10.7246376811594203No Hit
GTACGAGACATCGAACCCGTGACGGTCAAAGTTGGCGGCGAGAATCCCCT10.7246376811594203No Hit
GTTTTGGTGGGCGCTGCTGCTGACGCCACTGCTGATGGTGGTGGTGGGCT10.7246376811594203No Hit
AGCAAAAACAGCAGCATGCCCAGGCTCAAGCCGATCACGATAGAAGACAA10.7246376811594203No Hit
GTCATAAATCAAAGAATACCAAGAGGTACCAGAAGCTGGAAGAGACAAGG10.7246376811594203No Hit
GCATGGGCCCACGTCAATAAGGATGTTCCCGCGCCCTCACAGTACCTAGC10.7246376811594203No Hit
GTCACAGACGGGTGATTTATATTTGCAAGCCGAGCAAGCACAGCTTTTAA10.7246376811594203No Hit
CTAGTAAAGAGCTTGAATCAGTAATTAAGAGTCTCCCATCAAAGAAAAGC10.7246376811594203No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10.7246376811594203No Hit
AATATGGGCATACAATCTCTCTCTTCTGCGCTCGTATGCATCTCTATGCG10.7246376811594203No Hit
CCTCCATGATGCTGCTTACATGTCTCGATCCCACTTAACTATCTTGGGCT10.7246376811594203No Hit
CGTTAAGGCTGTCGTCGAGGCTGTCGGATCGGATGCTGCGGTTGTAGCCG10.7246376811594203No Hit
CTTTAAGTCTATTTAAAGAAGGCATGAATAATGATCCTATCCATGCCCTT10.7246376811594203No Hit
GAATGACCCCTCGTCAATGGCTTGGTTTAACCAAGGCCCGAAGATCGGTT10.7246376811594203No Hit
GCCTACTAATCATCACGGTGGGGATTTGCTTGCTGGCCTTACCAGCTCCC10.7246376811594203No Hit
GTTCTCAGCCTTGGTTTCGCCGGCGTTGTCAACGGTTCGCGTCAATCAGT10.7246376811594203No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA10.7246376811594203No Hit
GTTTAGACAATGTATTTTTCTGTTCCTTAACACTTCAACCTCATGATGAC10.7246376811594203No Hit
ATAAACAATCGTACACCAAGCCATAGCGGCATTGAATAGAAAAAAACCAC10.7246376811594203No Hit
GGGCTCCTTATGTGTCGAGCGCCCTGATCATCCTGGTCGGGCTCTACGTC10.7246376811594203No Hit
GCGTCAAGGGATGATTTTGCAATTGCCATATGAGCAATAATGTAAGCTTT10.7246376811594203No Hit
ACCTGGAGGGACGGGCTCACCTCGTCGCCTCCGGCAGCTCGGCGTGTCCT10.7246376811594203No Hit
CTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAGGA10.7246376811594203No Hit
AGGCGACGGTTGGACAGGCCACCCTTCTTCGCCAGCGTGATCAGCTTCTC10.7246376811594203No Hit
GAATCTCTGCACATTATTCTGTCCTTCCAAGTGGGAGAAGCATGCTGACA10.7246376811594203No Hit
GTGTACCGACAGGGCGGGCCATTAAAATCTCAAGCGCTTGATCAATCGTA10.7246376811594203No Hit
GCCACGCTCCCGGCGCAGATCCTTGAGGGTGTCGACGGTGTACGTGTCCC10.7246376811594203No Hit
CCATATAGAACGCTTGTTCTGGGATGTGATCGTATTCACCAGCAAGAAGA10.7246376811594203No Hit
GGTCTGGCAGGACGCGAAAGGCAACAGCCGGCTGTCGCTGGCGACGCGCT10.7246376811594203No Hit
GCCCTCTCATGCCATCATGACTAAACTGTTTTCAGCAACTCCCAACTTGA10.7246376811594203No Hit
CCTCTGCTCTAAACTGTGCTCCAGTTTTCCTTTCTGGCAACTCTTTTGAT10.7246376811594203No Hit
CCACTATTACCACCTGATCTCCCTCACTCACCAGCAAAGTAACTATCACT10.7246376811594203No Hit
TTTGGGTTGTTTCTACCTTTTATCTGTTTTGAAAAATACTGCTGGGAGGC10.7246376811594203No Hit
GTCGTACGTCGCGCAGTCCGCTCGCGGCATCTACCAGCCGGCAGTCCCGC10.7246376811594203No Hit
GGATAGATTATGGGCATAACTATTACTATCAATAGCTAGTTCCACACTAT10.7246376811594203No Hit
GGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGG10.7246376811594203No Hit
TGCTTCCTTTTAGTGAAGGTGATTTTCTCTGGTGATAGGATTTAGTTTCT10.7246376811594203No Hit
CGATGTGAATGGCTCCTCTGCCGAGGGACGCATGTTGCGCGCGTGTGCGC10.7246376811594203No Hit
AGGCTGTCTTGCAGAGTATGTAGTGATTGTCCTGGATGGCAGCGACCACA10.7246376811594203No Hit
ACAGAAAGCAGGAAAAATCTAAAATTGACGCCCTAACATCACAATTAAAA10.7246376811594203No Hit
TGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTC10.7246376811594203No Hit
TGTTCGCCTTCTGCCTTACCTCATTCCCACACGTCGCATCTCCGTCGTCA10.7246376811594203No Hit
GTGGCGGCCCTGCTAGGGGTCTCTGACGACACAGTGCGTCGCTGGATCGA10.7246376811594203No Hit
ACCTTACGCTCATCGTAACGCATTGCTTCAGCGACACGGCGGCTCAGTTT10.7246376811594203No Hit
TATTTGGACTTCTTTGAGGCCTTCGTTGGAAACGGGATTTCTTCGTATAA10.7246376811594203No Hit
GCAGATGTGTGATCATGGCTCACTGCAGCCTCGACCTCCCTGGCTCAAGT10.7246376811594203No Hit
GGTAAGGAGTTTCTGGTTAGCAGGCGCTATTACTGTCGCAATCGGTCCGG10.7246376811594203No Hit
CTATTAAGAAGCAAGGATTGGCCCCTCAAGCTGAGACATCTGGCCATTTC10.7246376811594203No Hit
CTCCTCCACAGGAGGATAGAGATCACCATCAACTGTGGATACAAGCATCT10.7246376811594203No Hit
ATGCTCATGATAGTAATCAAGCGCAAACATTGCGCCATGGGACAGTATCC10.7246376811594203No Hit
ATATAATGCTAGACAGAAGAATTCTCAGTAAATCCTTTGTGTTGTGTGTA10.7246376811594203No Hit
AATCAATTTAAACTTTCTAAATATTTCAACCCTGCTTTTCATGACAGATA10.7246376811594203No Hit
CCGTAGATAAGCTTCGACTCCTCATCGAACGGCAAGAGGCGCGGATCCAC10.7246376811594203No Hit
GCCCTGGGATGAGCCTCTGCTGAATGTACTCAATGTGTTCCAGGAAGGCC10.7246376811594203No Hit
ATCAAGCAGTATAGGATCACCAGATAACAAAGTGTTAACATTTATGCAAA10.7246376811594203No Hit
CAGGAAAGCATGGAAAAAAGCTGTGGGGAGTGTGGTTTGCTTGCCTCTCA10.7246376811594203No Hit
GTGATATCCCCTTTATCATTTTTTATTGCATCTATTTGATTCTTCTCTCT10.7246376811594203No Hit
CCTGACCTCAGGTGATCTGCCCGTCTTGGCCTCCCAAAGTTCTGGGATTA10.7246376811594203No Hit
GGATTTCACCATGTTGGCCAAGCTGGTCTTGAACTTCTGACTTCAAGTGA10.7246376811594203No Hit
GTCCCACAGGGTGCGAAATTCCAGGGTGTCAAAGACCGCGAGAGTAGCCT10.7246376811594203No Hit
GGCCTATTTTTTAGATGGATTAATATTTAAGTCAGTAGAGAGTAAAGCAG10.7246376811594203No Hit
GAGAACCATAATCCAAAAATGCAGCAGCTAAACTCATCATGTACTCTTGT10.7246376811594203No Hit
CTGTTGAGGACCGGCAAACCTTCCTTACCGATGTTCGTGATGCCCTATGG10.7246376811594203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)