FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.39159109645507006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.3297609233305853No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100.2061005770816158Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2061005770816158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA90.18549051937345426No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA70.1442704039571311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.1442704039571311No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG60.12366034624896949Clontech Universal Primer Mix Long (96% over 26bp)
GGTTTACTGAGAGCATTAGGGGCAACAAGGGAGCCCTGGGCATCTGGGGA60.12366034624896949No Hit
CTGCGGAGCCACTCCACGCACGTGCCCTCCAGGTAGGCTCTCAGCTGCTC60.12366034624896949No Hit
CTCCAAGCCCAGCCCTCAGCCATGGCATGCCCCCTGGATCAGGCCATTGG50.1030502885408079No Hit
GTATTTCAACTGCTGTAAAATTATAAACTTTTATACTTGGGTAAGTCCCC50.1030502885408079No Hit
CCTCGGAGCTGCTCTGGCTGCGCGCGGAGCGGGCTCCGGAGGGAAGTCCC50.1030502885408079No Hit
GTATTGGGACCGGGAGACACAGAAGTACAAGCGCCAGGCACAGGCTGACC50.1030502885408079No Hit
GCTTGAGCTGGGTCTTGGATATTTGGTTCATTTAGAAGTTCCTGTATTCC50.1030502885408079No Hit
GTCCTCCACCTGTCCGCTACGCTCGCCGGGGCTGCGGCCGCCCGAGGGAC50.1030502885408079No Hit
GTACATGGGAGTCTCGCGCCTGCAGCCCGTGCCGCCCCAGCCGCTGCCGC50.1030502885408079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)