FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGCAGCAGGCCCTGGCTCTTGCGCTCCTCGGGGGCCAGGCCCACT31.4925373134328357No Hit
CAGTTATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG31.4925373134328357No Hit
CGTTGGCGCCGAGCACGCTCTCGGGGAACAGCTGGCGCTCATGCACCGGC31.4925373134328357No Hit
GCCATTACTGTGGCACAGTAGGGTAAGTTCAAACTAAGAAAAGTACACAC20.9950248756218906No Hit
CTGCCGCGCTTCTGACCTGGAGTCACACCGTCGTGGACGTCTTCAACGAT20.9950248756218906No Hit
GAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGG20.9950248756218906No Hit
GACCCGGCCGTCTACCGCGACCGCTACCCGCGCGAGCTCTCCGGCGGGCA20.9950248756218906No Hit
TTCCACCAGGGCGACGACGCGGCCGGGATCATCGCGCACGCCGGCGACCT20.9950248756218906No Hit
GTGCGAGCGAACTCCTGCACGATCCGCTCGCAGAACGCCGGCAGGTCGTC20.9950248756218906No Hit
ACTTAGGATACAATTAGAACAACTGTAAACCAAATCAACTCTGAGTTTCA20.9950248756218906No Hit
AGTGATACCTACCCTGTCTATCTTAAAAGGTTATTGTGAATTTCAGAAGA20.9950248756218906No Hit
GGCCTCGTCGCCGCTGATGTGCTGGCCCAGTTCGGCCAACGCCTGCTGCG20.9950248756218906No Hit
TCCTCGACCTCGGCGGCGTGGCCGGTGATGGTGAGGAAGACGTCGTCGAG20.9950248756218906No Hit
ATCCAGTAGCCGGCGATCAGCTCCTTGGTCTCGGTGAACGGCCCGTCGAC20.9950248756218906No Hit
CTTTCCTTGCAGCGTGGCCACTTAACCCTGCGTGGGGGTAGGCGCGCACC20.9950248756218906No Hit
GACCAAAATGAGACATGTTTCTATGTGAGAAAATAGCCACATCCTCAGAG20.9950248756218906No Hit
CTGCCTGTTACCCAGTTCCAAACTTGCTTTCATATTTTTGGCTATCTTTT20.9950248756218906No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCTCC20.9950248756218906No Hit
GAGGAGAGCATGTGATAGCAAGGGAAACGTGTATTTAATGTGCTCCCTGC20.9950248756218906No Hit
AGCCGTAGCTGTTGAAGTCGGCCTTCGAGACGCCGTGCGCCTTCAGCCAG20.9950248756218906No Hit
ACCAGCGCCTGCACAACCTGGGTGCACAGCGAGGCGAGGTCGGCGGCCGC20.9950248756218906No Hit
ACATAAAATAGATTTCCTTGTATATAAATAACTTACATACGCTCCATAAA20.9950248756218906No Hit
GCAACATATAAAAAGAATTATACACCATGACCAAGTGGGTATTTTACTCT20.9950248756218906No Hit
ATCTACCGGGAGCCGTTGTCCCTGGCGGCCTTTGACCGGCTGCCCGGCGG20.9950248756218906No Hit
ATACCACAGGCAATGCTCCAGGCCTGTGTTGGCTCCTGGCTGGACAGGGA20.9950248756218906No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC20.9950248756218906No Hit
TCCTGCGCGTGGCCGGCGGGCGCCGCAGGGCGAGCCCGACGGGCACCATC20.9950248756218906No Hit
ATCTGGCAGCCGCGTCGCACACGCGGCTCGTTCTGGTGAACGCGGTTGGT20.9950248756218906No Hit
GTAAAACTAAATAATCTTGCTAAACAATAGAGGAAAAAAATTGTTTTGAT20.9950248756218906No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAAATTTAATTGTC10.4975124378109453No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT10.4975124378109453No Hit
TGTATGTGTTTGTGTCTTAGTTTTTAACAAAAAAGTTTAAAAATAAAAAA10.4975124378109453No Hit
GGGTTGTACTAACAGGAGTGCTTTGTCTTAGTCCCATCAGAGATAAAACA10.4975124378109453No Hit
GCCTAATGTCAACTAAACTCATAGCTGGGGTAGTCGGTCTATTCACCACC10.4975124378109453No Hit
AGGCTGGAGGGCAGGGGAGTGATCACAGCTCACTGCACCCTCAAACTCCT10.4975124378109453No Hit
AGGCAGGAGAATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAG10.4975124378109453No Hit
GCCCTGCGCGTTCGCGGGCACGCTCGCCGAGGTGACGAGCGCGCCGCCGC10.4975124378109453No Hit
CTCCGGTGCCGTCTCGGCTTCCACTCCTACGTGCGGCAGCACCCCGCTGA10.4975124378109453No Hit
CGGTGAATCTGAGAGGAGAGGATGGGGTAAGGCAGAAGCACCAGCTGTAC10.4975124378109453No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGT10.4975124378109453No Hit
GCCCATGGTGCGGGCCAGCTGGATGCCGTTGATCGACAGGGCCTCCTCGA10.4975124378109453No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG10.4975124378109453No Hit
GTCACAACTGGCACTGGTTGAGTACAAACTATATGCCAGACCGTTTTAAA10.4975124378109453No Hit
GTCGAGCGCCGGGTGGCCCGGGAGGCCCCCGGCCCGGCGCGCGGCGTCGC10.4975124378109453No Hit
ATCCACTGCGTCACCACCTGGTCGAAGTTCGACACCTGGTTCGCCGGGGT10.4975124378109453No Hit
GACCTGGTCGAGGGGCTGCGCGACGACCCGGACACCGGCCCGGTGTTCGT10.4975124378109453No Hit
CCGTTGCCGCCCGGCGAGGCGGATACGACCACCACCCCCCACAGCGCCCC10.4975124378109453No Hit
GCCATCTCCTTTTTTTTTTTACATAGGCACACCAACATGCCCACATAACG10.4975124378109453No Hit
GGTGTGGGGAGCGCGCCGGGCGGCCAGCGGCCGGAAGAGCAGCACCGCCG10.4975124378109453No Hit
GCCGACCGCCGCCCAGACGTCGTCGACGACGCACACCTTGGCGTGCACGT10.4975124378109453No Hit
GGACCCTCCACTTCTCCATCTACACCTTCACCAGGGATCCATATCACCCA10.4975124378109453No Hit
GTAATCATCAGATCTCACAAGTTAAAGGGCTCAGTCCCACAACACTGCCA10.4975124378109453No Hit
GACTCTCTCTTTGACCAAACTCCAGCCAGGCTCCTCTGGGCCTCCTGTTC10.4975124378109453No Hit
CCGGAAATCCATTGCCCGTGTTCTCACAGTTATTAACCAGACTCAGAAAG10.4975124378109453No Hit
CTCCTCCCTCGAGGACTTCCTGGTCGGCTTCTGGGAGGGGTACTGGCTCG10.4975124378109453No Hit
TCAGAGAACACAGAGCCCCCGGAAGAATTTTATGACATTTCAGGCAAGCC10.4975124378109453No Hit
GTTCAAGACCAGCCTGGGCAGCAAAGTGAGATCTCCGTCTCTACAAAAAA10.4975124378109453No Hit
GGCTTAGGAATGAAGAGAAAAAAGTAAGTAAAAAATAGAAATTGACTCCA10.4975124378109453No Hit
GCTTGAGGATTGCGAACCCAGAACACATATCCAAATTGCCCTGAATATAC10.4975124378109453No Hit
GTACTAATATGCATTCTCACCAACAATGTAAGAGTTCCCTTTTCTTCACA10.4975124378109453No Hit
AACCAGCGCAACGGCTACGTGCTACGTCGCGGCGAGACCGACGCGCCGGC10.4975124378109453No Hit
TCGCGGTACTCGTCGGCTGGGCCGTCGCAGCCAGCGGCCTGCTGGTCTGG10.4975124378109453No Hit
GCTCATACAAGATAAGAGAGGAGATAAAGTTGAATCACATAAAATGTCCA10.4975124378109453No Hit
GTGCTGGCGGGCGGTGCGGGCCACCCCGGTGGGCTCCCAGTGAGCGGCCT10.4975124378109453No Hit
CTTGCTGTTCTGCCCTCCACCATGGGATGAGTCAGCAAGAAGACCCTTGC10.4975124378109453No Hit
CCCCTCAAGGCTCCTCACTAGCAGTGGGCTCAGAGTAATAAAAATCCAGC10.4975124378109453No Hit
GCTTTAAATGACGCAATTCTCCCTGCGGCATGGATTTCAAGGTCTTTTAA10.4975124378109453No Hit
CTGTGGCAAAACCAAGATGAAGAGACGAGCTGTGGGGATCTGGCACTGTG10.4975124378109453No Hit
AGCTTCCTGAGTAGTTGGTATTATAGGTGCCCGCAAACACGCCTGGCTAA10.4975124378109453No Hit
CAGTAATAAAATATCCAGATTATCCTCCCAAATACCTGGAAATTAAAAAC10.4975124378109453No Hit
TGATTACATTCGATTCCATTTGATGATTCCATCTGATTCTATTCGAGGAT10.4975124378109453No Hit
CTACTAAAAATACAAAAAATTAGCCGGGCGTAGTGGCGGGCGCCTGTAGT10.4975124378109453No Hit
CTGGTTAATAATCTTTTTTCATATGGATAGTTCACCCATATTTCTGAAGA10.4975124378109453No Hit
GTGCAATGGCTCAATATCAGCTCACTGCAACCTCCACCTCCTGGGTTCCA10.4975124378109453No Hit
CATCCCGATCACGAAGCCGATCTACGCCCGCTACCGCGCGCTGGTGGAGG10.4975124378109453No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT10.4975124378109453No Hit
TCCGGGTCGTCGAGCGCGATCGTCGCCACGCCGCCGGGAGACACGCTGGA10.4975124378109453No Hit
GTCACGGTGAGCAGCCCGCCGTAGTAGCGCTCCACGGCGGACAGGCCGCG10.4975124378109453No Hit
CGGCCGGCCCGCGCGCTGCACCGGGATCGCCGCGGCCCGCTCGGTGACGT10.4975124378109453No Hit
GCCCCGGCATGACGGACGCGGAGGAGGCCCGGGACTTCATCGAGCGCCTG10.4975124378109453No Hit
AGCCCATGTTGAGCCCGAACAGGATGACCCCCTCCGCAAGCCACGCCGCG10.4975124378109453No Hit
GCCTGCTCGGTGACCCGGGCTGCGGCGAGGACGGCGTCGAGGTCGAGCCC10.4975124378109453No Hit
CTCCAGCTGCTCGCGCCGCGCCTGCTCCACGGGGTCATCGGTGTGCCGGC10.4975124378109453No Hit
GGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA10.4975124378109453No Hit
CTCCTGGCCGGGGAGGTCGGGCACGTTCGGCTTGCCCGACATGCCGGTGG10.4975124378109453No Hit
GCCCATAAGAGAAAGCAGGAAAGATCTAAAATAGACATCCTAACATCACA10.4975124378109453No Hit
GGATTCAATAGTATTCACTAACTGCTTATTACATGAGCAATTTCATCAAA10.4975124378109453No Hit
GTAATATATTGTTACTAAAAATAGCAACAGCAATGATAAACTTAGAAAAT10.4975124378109453No Hit
TGTAATCCCAGCTACTCTGGAGGCTGAGGCAGGAGAATTGCTTGAACCCA10.4975124378109453No Hit
GCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGA10.4975124378109453No Hit
CAGCAGCTTGGCGCCGCCCACCCCGCCGGCCAGCGCGACGATCTTCCGAT10.4975124378109453No Hit
TCGGATGGCCGTGTGGGGCGATCGGGGGACAGGAGAACCGGGTCTTCGGG10.4975124378109453No Hit
GACTATATCAGAGACTTGAAAAGGATCATTCTCTGTTTTCTGATAGTGTA10.4975124378109453No Hit
GTCTTTATCTCCTTCAATTCTGCTCTGATCTTATTTTGTGTCTTCTGATT10.4975124378109453No Hit
GCAGATGAGCACGCTCTCGGTGGGCGGCGACTTCCGGGCGATCGGCGGGC10.4975124378109453No Hit
GATGTACCTGGCGCGCGATGGCCGCCCGGTACCGTTCATTGCCACGCCGC10.4975124378109453No Hit
ATATTGTGGTGTGGAAAGCACTTTAACAACTGAAATTCACAAAATCTTAA10.4975124378109453No Hit
CGTTTTCCTTGCGCAGGCTCTCCGACGCCTGCTCGAACGGGGAGACGGCG10.4975124378109453No Hit
GACTAAAGAAGCAATATATGCAAAGATAAACGTAAATACACCTAATTTAT10.4975124378109453No Hit
GTATTTCATTTCTAGAGAACTGCGCCGTCTCTGTGGCGCAGTTCTCTAGA10.4975124378109453No Hit
CGCTGAAGTACGTCATGGTCCGCGCGCAGATGAGCGACTAACGCCTCGGC10.4975124378109453No Hit
GTATTCTTGGGTAAATATGTGTGTGTGTGAATTTTTTAATTAACACTTGG10.4975124378109453No Hit
ATACCAAGCATCTTAGAACTGTCATAAGATAGGTCATGCCTGAAATAGAT10.4975124378109453No Hit
CTCGCCGTCCACCTGCGGCGTCACGCCGATGTGGTCGTGGCCGCGCAGGT10.4975124378109453No Hit
CCTACTAGGAAAAAAAACAAAACTGCGATAAAGCTGTAAGACTAGCCTAG10.4975124378109453No Hit
ATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATC10.4975124378109453No Hit
GTGGTGGCATATGCCTGTAATCCCAACTACTCAGAAGGCCGAGGCAGGAG10.4975124378109453No Hit
CCCGGCGGGAACGGCCGCGGCGGCGACGAACGCCGAGCGGGCCGGGTCCA10.4975124378109453No Hit
GGCCAGAGCTCGTCCGGCACGATCTTCTCCAGGCGGTCCGCCACCTCGCG10.4975124378109453No Hit
CTATAGGGGATCAAGGATGTTAGCAGGAAGTCCAATTTAGCTGCAATTAC10.4975124378109453No Hit
GCTATATTAGGCTCCTTTCCCTGTTTTTTTTGTTAGGATTGACTGATTAA10.4975124378109453No Hit
GTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCC10.4975124378109453No Hit
GCACTGTCTTTTGATTGCAGAAATGACCACCAGAATTGGAGCTAAAGGAG10.4975124378109453No Hit
GGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGA10.4975124378109453No Hit
CCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCA10.4975124378109453No Hit
GACGTCCCCCGGCCCGAGGAACTGCCGGTAGAAGCGCACCAGCCGCCGGT10.4975124378109453No Hit
AATGCTTCAAAATACATGATACAAAAATTGATAAACTGCAAAAGAAATGG10.4975124378109453No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC10.4975124378109453No Hit
TCGTACTGTTCTTCGCCTCCGAACGACTGCTCACCGGCGGGCTCACCGCC10.4975124378109453No Hit
ATGTCTAGTTTCATTTGATTTTCTCTATTGTTTTTCTGTTTTCTATTTCA10.4975124378109453No Hit
GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCC10.4975124378109453No Hit
CCTTTTTATTTCTGTAAGGTAGACAGTAATGTCCCCTCTTTTATTACTGA10.4975124378109453No Hit
CAGCAGCCGACAGCCACGAGACGCCGGCGCCGACGTCCTCGCCCGCGCCG10.4975124378109453No Hit
GCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCC10.4975124378109453No Hit
ATTTTAGACACTGCACTAGCTGATGCTGCACGCATCTCATGTCAAGAAAT10.4975124378109453No Hit
GGATCACGATGCGAGGGACGTCGGCGCTGGTCACGGCGGGGGCGCTCGTC10.4975124378109453No Hit
TCCCTGGCCTCCGGCCGGCTGCTGACGGATCGCGGCAGGGCGCGGTGTCG10.4975124378109453No Hit
ACCTTTATGTGGTAGTAGATGGATCAAGAACAATGGAAGACCAAGATTTA10.4975124378109453No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTA10.4975124378109453No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG10.4975124378109453No Hit
GCTATGAAGAAATACTTGAGAGTGGGTAATTTACCAAGGAAAGAGGTTTA10.4975124378109453No Hit
GGCGGGGGCAGCAGCGGTCACAGGCCTGCCGCCGCCAGGACGTCGAGGTG10.4975124378109453No Hit
GTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAG10.4975124378109453No Hit
GTGTGGAAGTGGTACAGCGTCCGCCCGGTGATCAGCTGGAACGGGTACTC10.4975124378109453No Hit
CTCCAGCATGACGACGGGCGCTTGCTCTGGGGCACCGGCGTGGACCCATC10.4975124378109453No Hit
AAATAGAATCATCATGGAATTGAATCGAATGGTTCACTGTCGAATCAAAT10.4975124378109453No Hit
ACTCCATAGATATTAATGGCTATATTCTTGTGTATACTGTTACATCAATC10.4975124378109453No Hit
GATTTATACAGAGCCCTGTAAACTCTATATTTATTTCTGGGGAAATTTGT10.4975124378109453No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT10.4975124378109453No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG10.4975124378109453No Hit
TCCTCGCAGAGCAGGGCATCCGCTTCGAGAACAGTGCGAGCGAAGCCACC10.4975124378109453No Hit
GCTTGAGGATTGCGAACCCAGAACACAGATCCAAATTGCCCTGAATATAC10.4975124378109453No Hit
CATTTAGGATTAAACAAATTTTATTGGGGGTCTGAAGTAACTCCCCAAAC10.4975124378109453No Hit
CGGTCTAGCGGTACGCCCGGGCGTTCTTCGACCGCGACGCCGCCGACATG10.4975124378109453No Hit
GGCAATGTTCAACTCTGTGACTTGAATGCAGACATCACAGAGCAGTTTTG10.4975124378109453No Hit
CTATTTTCCTTTGTACTTGTGTTTTCTCAATTTGTTCTTTTCTCCTACTG10.4975124378109453No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGCCCTTCTTCCGGCGCAGCCGGTGCGGGTGTTGGTGCGGTCGATG10.4975124378109453No Hit
TCCTCCAGCTCCTCTTTGGTCATCATCTTCTTGTACTGGTTTCCCCTCAG10.4975124378109453No Hit
AACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTG10.4975124378109453No Hit
GAGCTCCTTTAGTATCGAAATTTTGAATTCTTTTTCTAGGATCTTATGAA10.4975124378109453No Hit
CACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAA10.4975124378109453No Hit
CAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT10.4975124378109453No Hit
ATTCCATTCCATCCCATCCCATTCCATTAGGTTCCATTCCATTCGTGTTG10.4975124378109453No Hit
GTGGTGGCACATGCCTGTAATCCCAACCACTTGGGAGGCTGAGGCAGGAG10.4975124378109453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT10.4975124378109453Clontech Universal Primer Mix Long (96% over 26bp)
CCTCGGCCGCCCTGCCGCTGCTGTTCCGCGCCGTCCACGAGGACGGCAGT10.4975124378109453No Hit
GGGTTGCGCCGTCCCGACCTCGACGACGTCTTCCTCACCCTCACCGGCCA10.4975124378109453No Hit
GAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGG10.4975124378109453No Hit
GGCCATCACGCGCCACTCCGACACGGAGAAGCCCTGCTGGCGCGCCACTT10.4975124378109453No Hit
GTCGCGCTCGGCGTCGCCGCCGCGGCCGCCGGGCTCCGGCCCCGGGACGC10.4975124378109453No Hit
ATTTAAATACCTTGATTTTGAAAGTCAGATTGCCCACAAATTCTGTTAAA10.4975124378109453No Hit
CCGCAGCACTGGACATCGCTCGTTTCATGGACCCCATCTGAGCGCCTCCG10.4975124378109453No Hit
GATTTAAGGGAAGAGAAGAGGGAAGAAGAAAACAGCCAACTTTAAGCAAA10.4975124378109453No Hit
ATATAAATGTAAGTGGGATTTCATGTGAAAAGGAAAAAAAAATAGGGACA10.4975124378109453No Hit
TCTCCCAGGCGATCTACGAGAACGTCAACGCGGCGCTGGCGGGCGAGATG10.4975124378109453No Hit
CTCCAGGAGACTGCTGATTTTGGCATTCTTTTTCCTTTCATCATATTTCT10.4975124378109453No Hit
GGTCAGAAATGTCCCTTTGTACATTCTACCAAAAGAGTGTTTTCAACCTG10.4975124378109453No Hit
GCACGTGGCCGACCCGGGTGGTGATCTCCGGGCCGATGCGCAGCGTGCCG10.4975124378109453No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)