FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGACGGTGAACGGCCAGCTGGTCAACGTCGAGCAGTTCAAGAACATC33.296703296703297No Hit
CAAAAACGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGA33.296703296703297No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG33.296703296703297No Hit
AACATGCAAACGCCAACCATGGGACGATTGCGACTTGTGTCTGAGAAGAA33.296703296703297No Hit
AAAAAAATCTGCATTCAATGTGCAACATCACAATCAGTAGGGCATTCTGT33.296703296703297No Hit
AATCTCATTTATCCAAGCAGGAGTTGTACAAGGGAGTTATTTATCTTGGG33.296703296703297No Hit
GGCCCCAACGAGAACCTGCACCTCCTGGACCTTAGCTTTCTGGTCTGTAC33.296703296703297No Hit
GGTGAAGCATTGTGATAAATTTTTCCACAATTTTTTTAAAAAAACTCAAT22.197802197802198No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC22.197802197802198No Hit
TCGACGGGCTGGGTCCTATCCGCGGCGCCGGCCGCTGGCCGCTGGCCGGC22.197802197802198No Hit
GAGATGGTTTCATCGACAAGGAAGATTTGCATGATATGCTTGCTTCATTG22.197802197802198No Hit
CACACACACACACGCACACGTTCAGATGCATTCAATAACATTTTCAAATA22.197802197802198No Hit
GAGCAGGAGCGCGAGCTCGTGTGGCGTCAGACCAAGATCGAGCGGCTCAC22.197802197802198No Hit
GTCCTTGTAGGTGTCGAAGCCCAGCGGCAGCACCAGCGCCTGCGGCTGGA22.197802197802198No Hit
AATGGAAAGAATGGAATCAACACGAGTGGAATGGCATGGATTGGAATGGA22.197802197802198No Hit
GAACTATCCTTTACACAGAGCAGACTTGTAACACTCTTTTTGTGGAATTT22.197802197802198No Hit
AGCCAAGGCAATCCTAAGCAAGAAGAACAAAGCTGGAGGTATCATGCTAC11.098901098901099No Hit
GTCCTACGGCTACGGGCTGTCGGCCTCGCTCTTCCAGATCGCGCACGCCT11.098901098901099No Hit
CAATTGGGATATCCCAGTGCCTTGGGCCTTGGACATTAATTTAGCATCTG11.098901098901099No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG11.098901098901099No Hit
TCGACGGGCTGGGTCCTATCCGCGGCGCCGGCCGCATGCCCCATGCCGGC11.098901098901099No Hit
GTATGAGCACTTGTCACTTCTTTCCAGAGCTGCTGCAGTTCTGTTGGAAT11.098901098901099No Hit
GTCATAGTTACTCCCGCCGTTTACCCCCGCGTCATTGAATTTATTAACTT11.098901098901099No Hit
AATAACCACTTGCAGATTCTACAAAAAGAGAGTTTCAAACTCTTCTATCA11.098901098901099No Hit
GGATAAATTCCTCGATACATACACTCTCCCAAGACTAAACCAGGAAGAAG11.098901098901099No Hit
GTTTATGGTAGGTTCTATTGCCATTTGGCCGGCATACCATTTACATTTCC11.098901098901099No Hit
CAAAGAAGGCCATTACATAATGGTAAAGGTTTCCATTCAACAAGAAGAGC11.098901098901099No Hit
GCGCGGCTGTCGGCGAAGTCGTAGACCACCGCCTTGATCGGATCGAACGC11.098901098901099No Hit
CTTCCCATCCTCAAACATCAGAGTCCAACTTTTTCAGCCTTGGGTCTCGA11.098901098901099No Hit
AAGTAGTTTCTGAGAATGATTCTGTCTAGTTTTTATTTGAAGATATTTCC11.098901098901099No Hit
CTCCCGCTCTCTCAAAAGGAACTTGGGAAACTTGTGAAACCCAGGAAAAC11.098901098901099No Hit
GGCTCGACTCCAACAGCTCGTGCACCACCTCGCTGGCGCCCGCGGCCTTG11.098901098901099No Hit
ATTCCCTATTGCTAAATTAATTTTTTCAGATTAAATCATTTCTGACTCAT11.098901098901099No Hit
GGCTGGAGTGCAGTGGCACCATCTTGGCTCACTGCACTCCAGCCTGGGCG11.098901098901099No Hit
GATCGCCGACGGCAGCTACAGCTTTGCCAACACCGGCTCCCGCAGCTTCG11.098901098901099No Hit
AGAATTTGACATTGATTTTATACTGCCTACAATATTGTTTATTTAATGTA11.098901098901099No Hit
ATATTAGGTAGCCAGGTGAATGGGACAGGTTAATACAGGAACACCCTTCA11.098901098901099No Hit
GAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACA11.098901098901099No Hit
GAATGTCGGGATGCGGATCGAAGCTGCCGCCGGGCGGCAGATCGAGTCCG11.098901098901099No Hit
GGTGAAAAGGACTTGCCTTGTCTCAGATGAGACTTTGGACTTGGACTTTT11.098901098901099No Hit
TCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACGA11.098901098901099No Hit
CTTTCAAACTCTTTCAACAAACTTTCATGGAACCCTCGGTGTGTGTCCAG11.098901098901099No Hit
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT11.098901098901099No Hit
GTATTTCTTGTAAAGATGGCTTTCTAGCAACAAATTCTCTCAAGTTTTGT11.098901098901099No Hit
TTCTTGCCCTCGTGCGTCACCAATCGCGATCGGAGCACTTCGGCGTTCTG11.098901098901099No Hit
GAGCTGGGGAAAACGGAAATCATTTAATCAAAGCCTCAGAAACAGCCACT11.098901098901099No Hit
ACTCTAAGATATGAATAAAATGGACCAGGATATTTTCAGAAGGCCCCAAA11.098901098901099No Hit
CGCAGAGTACTTTTTTTTTTTTGAAAACAACAAATACTATAAGAACAAGT11.098901098901099No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG11.098901098901099No Hit
GTCCACTGCTCCTCCCGGGTATGTGGTGGCTTCACCGCGGTCGGGGGAGG11.098901098901099No Hit
CTGCTGTCATTTCAGGAGGCCGAATTTTTTCCCAATCCCAGAGAAGGTGT11.098901098901099No Hit
GCATTACAGACACCAGCTCCGGCAAGTTCTGCGGAAACAGCGAGGGCACT11.098901098901099No Hit
ACCGTGAACCGCGGCGAGGTCTTCGGGGTGATCGGCTTCTCCGGCGCCGG11.098901098901099No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA11.098901098901099No Hit
GGCAGCTAGGTCTTATCATCATTCCCAATTAGCAAATGAGGAGGCTGAGG11.098901098901099No Hit
CCTCATGACCACGCTTTCCTTCTCCCTCGCCCTTGGGGGAGAGGGAGCAA11.098901098901099No Hit
ATTTTAGGTGTTGGGGTACTTCAGTAACCAAAACACTTAAAAAAACAAAA11.098901098901099No Hit
TCTCTGTACAGCTCATCCACTTCCTCATCTGTAAACCGATCCCCCATGGT11.098901098901099No Hit
ATTTTGAAGAATGAGGGAGCATAAACAATTATGACTGCCTTTTAAAAAAT11.098901098901099No Hit
CATTTCATCAATACCTAGTTTATTGAGAGTTTTTAGCATGAAAGGCTNTT11.098901098901099No Hit
CAGTGTAGGGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGT11.098901098901099No Hit
GTGGAGGTGCACAAAACACACAGAATCCCAGCACTTTAGGTAATCATAGG11.098901098901099No Hit
CCTTTGAAACTCCTTTGTAATAATTTGATCAAGCTAACACACATTTTTCA11.098901098901099No Hit
ATTTATTAGCCCTAACCCTAGAGATTCTCAATAAAAATTCTGGGATGAAA11.098901098901099No Hit
TTACACCCACTCCCAATGCTATCGACTTAGTTCAAACTTCCTTTTCAGTA11.098901098901099No Hit
GGCAGCTAGGTCTTATCATCATTCCCAATTTGCAAATGAGGAGGCTGAGG11.098901098901099No Hit
GTCATAGTTACTCCCGCCGTTTAACCGCGCTTCAATGAATTTATTCACGT11.098901098901099No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099Clontech SMART CDS Primer II A (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)