FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG32.912621359223301No Hit
GCTCTATGGCACTACCTATCACCTGAGAAACCCAAATACTTATCCAGGCA32.912621359223301No Hit
GGCTCTGGCTAGCCCTTCCTGGGCTGGTGACTGAGGCCCTCCTCCCCTTA32.912621359223301No Hit
CCCGGCAATCGATAGCAGTAGTTACGGCATTATGTGAAAGGTTCGGTTGG21.9417475728155338No Hit
GATGATATGGAAGAAGGTCTTAAAGTCCTAATGAAGGCAGACCCTGGTAG21.9417475728155338No Hit
GTCTTCCAGCATCAGCGGTTTCCCTTCCACAAAACGAACGCGACGGGAGA21.9417475728155338No Hit
CTTAAAAATAGTGTATTAAATTTGAAAAAAAACAAAAACAAAAAAAACAG21.9417475728155338No Hit
GTGTGGAAAGGGCAAAACTATGGAGACAGTAAAAAGATCAATGGTTGTGG21.9417475728155338No Hit
ACTACATCCAGACAGTGAGATGCTAGACCCCTCTTTCATCTTGATTGCTT21.9417475728155338No Hit
ATTGTGTATACACATACAATGGAATATTACTCAACCGTAAAAAGGAACAC21.9417475728155338No Hit
GAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGC21.9417475728155338No Hit
CAAATCATGTTACCCTTCTGCTTTAAGCCTTCCAGAAACTTCGCATTGCA21.9417475728155338No Hit
GTTACCTACTGAGTAGGCGGCGATTTTTGTATGTTATGAACATGCAGTTC21.9417475728155338No Hit
CTCCTAAGCGATGCTCAAACACATTAGGCACAATCCAGGTGGCCTCTGCA21.9417475728155338No Hit
GGAATGGAATGAACCCGTGTGCAATGGAATGGAATCGAATGGAATGGAAT21.9417475728155338No Hit
CATATATCCTTTTGCAAATTCTACAAAAGATTGTTTCCGAATTTCTCAAT21.9417475728155338No Hit
CCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCC21.9417475728155338No Hit
AATTAACTCAAAATGGATCATAGACCTAAATGTATGTAGAGGCTGAACTA21.9417475728155338No Hit
CTACTGAAAAGTTCGTTTTTCAAACTTTGTCCCCCTCTCTTCCTCCTGCC21.9417475728155338No Hit
CATTACTCTTCAGCTAAAAGTACTCCAGTGGCTTCAATATGTACTAAGAA10.9708737864077669No Hit
GGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAG10.9708737864077669No Hit
ATGTTCGACGGCGCGATGCCGCAGGCCTCGATGTAGCTCGAGTAGCCCAT10.9708737864077669No Hit
GCATACATCGCTGAAAAGGTGGCCATGGAGAAGCAGCAGTGGCCTCATGC10.9708737864077669No Hit
ATATACTGTTCATAAAATATCGAACTCCCCAAAGTATGTTTTACCTACTG10.9708737864077669No Hit
GGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCG10.9708737864077669No Hit
GTGTTTGGAAGCAAAGATAACTTCCACGGCTTAGCCATCTTCCTGGACAC10.9708737864077669No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT10.9708737864077669No Hit
ACATTAAACACAGGAGTGTGCAATTCCCACCTATTTTCAGTAGTCAGTCT10.9708737864077669No Hit
GCATGGGGTCTCGGGAACAGCTTGCGATTGCAGAGTTTGCAAGATCACTT10.9708737864077669No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA10.9708737864077669No Hit
GAGACGGAGTCTTGCTCTTGTCGCCCAGGCTGGAGTGCAGTGGCACTACC10.9708737864077669No Hit
GAATGGGATTATGGAATGCAATCGAATAGAATCATCGAATGGAATGGAAT10.9708737864077669No Hit
GATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGC10.9708737864077669No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA10.9708737864077669No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.9708737864077669Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGACTGTGCCAATGCACTCCAGCCTGGGCTACAGAGCCAGATGCCATTAA10.9708737864077669No Hit
GGCCCAAAAAGTTAATAGGTGACTTGACCTCTCTTTTGTCCCTGCCACCT10.9708737864077669No Hit
GAGTTAAAAAGCACTGAACATTGGCGTCAGGGTCTGTCTCTTATACACAT10.9708737864077669No Hit
TCGCCCTGGATATCTGTTCCAAAAACCCCTGCCACAACGGTGGTTTATGC10.9708737864077669No Hit
GAATGGAGTCATCCGGAATGGAACAGAAAGGAATGGAATCTGTCTCTTAT10.9708737864077669No Hit
GGTGTTTATAGTATTCTCTCATGGTAGTTTGTATTTCTGTGGAGTCAGTG10.9708737864077669No Hit
CCCCTGCTCACAGGGCAAGAACGGGCTCTGCAAGGCTACGGCTGGGAAAA10.9708737864077669No Hit
GCCTCTGGTGTGTTTATCACTGTGCCAAGTGCTGTGAGTAATGGGTATGA10.9708737864077669No Hit
GCCTTTGAGAGCAAAAGCTGTATCTTACTTAGTTTTGCATCTTAACAGCT10.9708737864077669No Hit
CTTGAAAGATAGTGATTTTCCTTGGTAGATTGATAAACCCATGTTTTACA10.9708737864077669No Hit
GCCAAATAAACCTCTTTTCTTTATAAATTACCCAGCCTCAGGTATTCTTT10.9708737864077669No Hit
CGGTCGATACCGTGCCCTCTGGAATTGAGCCATCCGGGGCAATGGACGAC10.9708737864077669No Hit
GCAATGGGCAGGAGTATCAAATTCTCTCACAGGGAAAGCAGTGAATAATT10.9708737864077669No Hit
CCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTT10.9708737864077669No Hit
GAAAATACTAACAAAATAGACCACTATCCAGATTAATAAGGTAGAAAACA10.9708737864077669No Hit
CTGCATAAACAATCCAAACTACAAACCAACCTTCCGCATGCTGCAGCACA10.9708737864077669No Hit
GAATACAAACATCACAAAGATGTTTCTCAGAACGCTGCAGTCTGCAATTT10.9708737864077669No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG10.9708737864077669No Hit
GTGCTGGAGTTCTTCTGCGCGCCTTCGCCGCTGTGGCCGCGGTCGGTGCC10.9708737864077669No Hit
GTAACTATAGGCATGCACCACCACGCCCAGCTATTTTTTTTTTTTTTGGC10.9708737864077669No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT10.9708737864077669No Hit
GGAGTACAGTGGTGCAATCTCAGCTCACTGCAGTCTCTGTCTCCCAGCTT10.9708737864077669No Hit
TGACTATTGAATGGTTTTCCATTTGTTTCTGTTATCTATGATTCCTTTTA10.9708737864077669No Hit
GAACCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATC10.9708737864077669No Hit
CGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGA10.9708737864077669No Hit
TCTGAACAATCCATGAATCAAGATACTTAGGAAAGTGGTGATGGATTGAA10.9708737864077669No Hit
ATCTTACTGTATGTTTGTACCCATCGACCAACCGCTTTCCATCCTCCCTT10.9708737864077669No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG10.9708737864077669No Hit
ATTTTCAGCTGCTCTCAATACTCATGCCTACGATATTTTAACAGCCAGAA10.9708737864077669No Hit
GTAAATGGGCTAAATGCTCCAATTAAAAGACACACAGTGGCAAATTGGAT10.9708737864077669No Hit
GCGTATCGCTGTGGCAGCTTCGATTGTGGGGATCGCGGCAACGGCAAATG10.9708737864077669No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA10.9708737864077669No Hit
GGTTTCAATTTCTATCGCCTATACCTTCTGCATAATGAATTAACTAGAAA10.9708737864077669No Hit
ATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATCTACCAGA10.9708737864077669No Hit
GTTTATCACTGAGAGGACTGTTAGAATTTGAATCGAGGATCCTAATTTGA10.9708737864077669No Hit
GTGCTCATCGTGCCCGACCTCGACGCGGGCAACATGCTCGCGAAGAGGTC10.9708737864077669No Hit
TGAGTCCTGAATTGTCTATCCCCTTGGCATGTGAAACCAAGTGGCAAAAA10.9708737864077669No Hit
ATTCCGTACGATTCCATTCTGTGATGATTCCATGTGATTGCATTCAATGA10.9708737864077669No Hit
GGATATGAAATTCTGGGTTGAAAATTCTTTTCTTTAAGAATGTTGAATAT10.9708737864077669No Hit
GCCTAGACGGTCGATCTACGCGGGCGCTGGGCGGAGGAGGCCAGCGTCGT10.9708737864077669No Hit
AGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGT10.9708737864077669No Hit
TCCTTTAGGAAGCCACAGTCTACTGAGGGCAAATAGTTAGTGTGATACAT10.9708737864077669No Hit
CATATGGCCTGCAGACAATATAAAGCAATTACACAATCAAGTCTACAAAA10.9708737864077669No Hit
TCTCCGCCCAAGGGCATCGCCGGCGGAGGCGCAGGCGCGCTCAACGTGTT10.9708737864077669No Hit
GTACATGGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT10.9708737864077669No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG10.9708737864077669No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)