FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCTCTGGTTAGCCAGAATGTTACAGGTGGTGGAATTAGCTGTTGTTT32.912621359223301No Hit
CCCTGCTGCCTGCTTGCCCTGCCTACTCTCATCTGAATCTGCTATACTTG32.912621359223301No Hit
TGCTGGAAGAGCCGAGCGCAGAGTTTTCGCGCATATGGGCAATTGAACGC21.9417475728155338No Hit
CTGCGCCCAAACCGAAGTCATAGCCACACTCAAGAATGGGCGGAAAGCTT21.9417475728155338No Hit
GGCCAGGGGATCTGCACGTTGTGTTAGACATGGCTAAAGTAGCTGATACC21.9417475728155338No Hit
GTACAAGTACTTTGTTGGAGATATAATTTGCAAAGATATTCTCCTATTCT21.9417475728155338No Hit
ACAGTGTTGAACCTTTGTTTTCATTGAGCAGTTTCGAAACCATCTTTTTG21.9417475728155338No Hit
CCCATGGGCAATAAAGCGCATTCAATGTGTTTATAAGCCAAACAGTCACT21.9417475728155338No Hit
CATTTACACATCCTTATCATCCTAAATCCGTAGTTTGTATAGAGTTTGTT21.9417475728155338No Hit
GATCACCGTGAAGATATGGTCAGCTCTCCTGGAAGGTCAAAGGGCAGACA21.9417475728155338No Hit
CCCTAGGACCACCCCCACCCCCAGTTGTTAAACATTTAACAGCACACTAT21.9417475728155338No Hit
ATCTAGAGATGATCTACAAAAGCATTGCGGAGCGGTTAAGAATTCGACTT21.9417475728155338No Hit
CCATTACAATCGAGTTGATTCCATTCCATTCCATTCAATTCCGGATGATT21.9417475728155338No Hit
GCCCTGCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTG21.9417475728155338No Hit
GTTCTTTTTTTCTCAAAGAAATAAGTTTGGAGATTTCTCAGAAAGCAGAA21.9417475728155338No Hit
GCTATAATCGGATGCTGCAGCTGAGATGGAAGCTCAGTCTCAAATCCATC21.9417475728155338No Hit
GCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGA21.9417475728155338No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21.9417475728155338No Hit
ATCATACAATGTGTGGCCTTTTGCATCTGCCTTCTTTCACTTAGCACAAA21.9417475728155338No Hit
CTTTACAGTAACTATCTGTTTACTGTGTACTTTTCAACATGTTTGTTGTA10.9708737864077669No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT10.9708737864077669No Hit
CCTGAAAGTGATGGGGAGAATGGAACCAAGTTGGAAAACACTCTGCAGGA10.9708737864077669No Hit
GTCTCGAGGTTTACCATTGCTCAAATCAAGACCAATCCATTTTAGATTTT10.9708737864077669No Hit
ATGGAATCACCATTGAATGGACTCGAATGGAATCATCATGGTATGGAATC10.9708737864077669No Hit
ATGCAACTGAGGATACAACTGACCTTTTTGTTCTCTGTCAGGGTCTAAGG10.9708737864077669No Hit
GTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTG10.9708737864077669No Hit
GATGATTACTTTCGATTCCATTTGATCATGATTCCATTCGATTCCACTCG10.9708737864077669No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG10.9708737864077669No Hit
GCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAG10.9708737864077669No Hit
GAGGTAGTGCCACTGCACTCCAGCCTGGGCGACAAGAGCAAGACTCCGTC10.9708737864077669No Hit
ACCCAGGCTGGTCTCAAACTCATGGCTTCAAGCAATCCTCCTATGACAGC10.9708737864077669No Hit
GGTAAAATGCCACTAAACCCTCATATATGAAGACGTGCATTGTTTAGCAA10.9708737864077669No Hit
GTTGATAAGAAATCATGCTGAAAAAACAACTAATTTCACTCTACTAGGCC10.9708737864077669No Hit
CCTCACAAGACAGAACTGGTATGAGCAGGATTTCTGCAGGTTCTTCTTCC10.9708737864077669No Hit
GCCCCGGGAGTAGCGCACAGCCAGGAAGGTGTCGTGATCGCGGTTGCGGA10.9708737864077669No Hit
CCTCTTCCAGAACCCCTGGTTTTATTTTATAATCTAACTCGGCTGCAAAC10.9708737864077669No Hit
GTTGATTCGATTCCATTCCATTCCATTTCATTGCATTCCATTCCATTCCA10.9708737864077669No Hit
GTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGA10.9708737864077669No Hit
TATTTGGGCTAATGTCCCTAATTAGAGAAGAAGTGTCAGTCAAAGAAGGT10.9708737864077669No Hit
CTCAATCAGTCCCATGATTTTAAACACTATCTATGCTCATTATTGTCAAA10.9708737864077669No Hit
CCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCT10.9708737864077669No Hit
ATTCCATTCCTTTCTGTTCCATTCCGGATGACTCCATTCCTGTCTCTTAT10.9708737864077669No Hit
GTTAGGAGGGTTTTCTCAGAATCTATTCAGGAGAGTTCCCTTTAGATCAG10.9708737864077669No Hit
ATTATAGCTACTGTGTGCTGTGATAGGAGAATACACCTTTTCAAGATTGA10.9708737864077669No Hit
ATGGCGGGTCGCGCGCATCAAGGGCATCGTCTCGCCGGTGGCGGGTCGCG10.9708737864077669No Hit
ATAATCACTGCGCCCGCTCATAAGGGGATGGCCATGGCTAGGTTTATAGA10.9708737864077669No Hit
AGGATACATAAGCGTCAAACTTTCCGGCCTGAAGTGCCGGAATAGTGCCA10.9708737864077669No Hit
GGAATTGCCAGGAGAGGTCATTCGGGTGCTTTCTTCGTCTTGTCGCTGCT10.9708737864077669No Hit
GCGGGGCTGGGCGGGCCCCCCCGCCACCCCCGGCCCCCCCCCCACCCCCC10.9708737864077669No Hit
CTCCGGGACCCAATGCTGCAAACCCAAGAAGGTCACACGCACAGATGAGG10.9708737864077669No Hit
GGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCG10.9708737864077669No Hit
GGCAAGCGCAAAGTAATCCAAAAGCTAGCAGAAGACAAGAAATGACTAAG10.9708737864077669No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG10.9708737864077669No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9708737864077669Clontech Universal Primer Mix Long (95% over 21bp)
CTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCTAC10.9708737864077669No Hit
GTCTTGAACTCCTGACCTCAAGTGATCTGCCTGCCTTGGCCTCCCAAAGT10.9708737864077669No Hit
GGCGACACGACGCTGCACACGCTGATCGGCGAGCTGTCGGTGGCCAGCCC10.9708737864077669No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA10.9708737864077669No Hit
GCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAA10.9708737864077669No Hit
GTAATGGCCTCCTTTGTCTCTTTTGATCTTTGTTGGTTTGAAGTCTGTTT10.9708737864077669No Hit
ATAATTCAGAACACCCGAGGGACCAAATTTCCAGCATGTCCAGTTTTTAA10.9708737864077669No Hit
GTATAATTTTAGTTGGTTTAAAAAAAAACAAGCAAACAGTATTTGCCTTC10.9708737864077669No Hit
GACAATATGATTCTATGCATAGAAAACACCAAAGACTCCACCAAAAGGCT10.9708737864077669No Hit
CCAGATAGCGATAGTGCCATATCATGCTAGTGCTTTCATTGTGTGAGTTT10.9708737864077669No Hit
TATATTCACAATGGAATGCTATTCAGCCATAAAAGGAACTAAATTCTGTC10.9708737864077669No Hit
GTAGTCGCCCAGCTTGGGCTCGTCTTCTATGACCAGGATACGCATTGCCG10.9708737864077669No Hit
GGTAGCAAGGTAGAGATGATTTATACCTCAGGTGCTGTCTAGCTTTTTTT10.9708737864077669No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC10.9708737864077669No Hit
ATTACTACCTCAATTTGAGAACTTGTTATTGGCCTATTCAGGGATTCAGC10.9708737864077669No Hit
GAGTACATGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTC10.9708737864077669No Hit
ACCCTGACGCCAATGTTCAGTGCTTTTTAACTCCTGTCTCTTATACACAT10.9708737864077669No Hit
GTATACAAATTTTAGTATGTGTGGACATTCATGCCTTTTTCTAGGGAAAG10.9708737864077669No Hit
GGTTTGAAACACTCTTTTTGTAGTGTGTGTAAGTGGACATTTGGAGCGCT10.9708737864077669No Hit
CCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATT10.9708737864077669No Hit
TTGGAAGTCTGTGAGGTCAGCCTAGCCACCTATGTATTTTATGCCTCTCT10.9708737864077669No Hit
CTTTGGGTACTTTGATATTTTATGTATAGTATAAAATATATAGTTTGGGT10.9708737864077669No Hit
GTTTTATCTGATATAACAATATCAGTTCCTCCTCTTTTCCATTTGAATGG10.9708737864077669No Hit
CTCCTGCTTAAGCCCTTTCAAAGGCTTCCCATTGCTCTTAAGACAAAGAC10.9708737864077669No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC10.9708737864077669No Hit
GACAAGGCGCGGGTGCTGGCCGAGGTCGACGGCTACGCTGCGCGCAAGGC10.9708737864077669No Hit
GCAGATGCCTTGTCGCTGCAAGACAGTCCCGCCATTCCTGCAAACGCGCA10.9708737864077669No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10.9708737864077669No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)