FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACAGAGTTGAAGGTTCCTTTTCAAACAGCAGTTTCCAAACACTCTTT45.128205128205128No Hit
GGTTTGAAGGCAAAGACCTCAAAAATGAAGAAAAGAATTTCCTAATCAGT33.8461538461538463No Hit
ACCGTTCACTCCGTCAAGGCCATCCAGGCTGCCGGGTACGAGGCCATCAT33.8461538461538463No Hit
GCTCTACTGGCCCCACCTTTATTCTGGGCCCTGCCCTGCCATGCCCTGTT33.8461538461538463No Hit
CTGCTCGATCTCCCATTGCGCCTCGGGCTTGAGCTGCGCGAACAGCTTCG22.564102564102564No Hit
GTGCGCATCAGCCGAAATCGGACATGGATTTACTCCGTGCAGTAGGACTG22.564102564102564No Hit
GCGGCCTCCTCGGGGGTCGTGTGCTGGATCTTCCCGGTCATGAGCTGTAC22.564102564102564No Hit
ATGCGCGCTTCCACGCGGATCGAGAGTTCCTGTTCGTCAGTCCGTGCCAT22.564102564102564No Hit
ATCATGACGGGCGATCCAGTCTATTCGGCCCTCGTCAACGGAAGGACGCG22.564102564102564No Hit
GATGAATATCTGGTAAGACATGGAGAGGTGGTTTCTGTCATAGTGGGGTG22.564102564102564No Hit
CTCGAATATCAATCAGTACTTAATCAGCTCTTCTATCCAGGGGGCATTTA22.564102564102564No Hit
CTATTGCATACCTAGTGTTTTAAGCAGTATTTGCAAAAGTAGTATGCATG22.564102564102564No Hit
TTCCCATCGTTGCCCATCGTGCGGCGCCAGCGCTTGCGCTTCACAATCTG22.564102564102564No Hit
GCGCAGACGCTCGAAGCGCAGGCGCTTTCCATGACGACGCAGCGCGCCCA22.564102564102564No Hit
GTTCCAGTCCATTCATTTCTCGTCCATTCCATTCCATTTCACTCCATTCG22.564102564102564No Hit
GTGCTGGGAAAACTGGCTAGCCATACGTAGAAAGCTGAAACTGGATCCCT11.282051282051282No Hit
GTCATTTATTTATGTGATCATATCGGGCACATGGCTTGGTTACATCTTAG11.282051282051282No Hit
GCACATAACTTGGAGACAAACATAGTTACCACGTTCATTAGTGAAGTCTC11.282051282051282No Hit
GTCTGCCATGTTAGAAGAAAATTTTCCTATTGTATGTGAAATTTTCCCTA11.282051282051282No Hit
GATCTGAGAGGCGCGGTCGAGGCCTTCCATCTTCTCGATGATCTCAACGA11.282051282051282No Hit
CGTCGTCGGACGCCGGCCCGTTGAGGCCACCCGGCAGACGCGACGCGTCG11.282051282051282No Hit
CTTGTCGACCGAGCCGTAGGGCGGGTAGTCGGTGGGAACGGCGATCTTGA11.282051282051282No Hit
CCACAGCAGGGAGAACATGGTATCAGTTTGACACATTGTTTGTGATATTA11.282051282051282No Hit
ATGTAGATATTTACCTCTTTCTCCAGGTTTGGAAAATTTTCTGTTACTGT11.282051282051282No Hit
CATATTTAATGAAATAATAGCTGAAAAATTCCCAAGTCTACCAAGGATTT11.282051282051282No Hit
CTGAATTTGAATGCTGGCCTGCCTTGCTAAGTTGGGGGAGTTCTCCTGGG11.282051282051282No Hit
GCCCTGGCAGTCGCTGCCCGAACTCGTCGCGGCGGCCAAGGCGAGGCCGA11.282051282051282No Hit
GGCCTTGTCCACACGGTGGTAAGGAAGAAGAGAGTGTCCACAGGGTGCTT11.282051282051282No Hit
TCAGAGGACAGTAACTTTGAATAAATTTTTAGAGGGTTTTTTGGTTTGGT11.282051282051282No Hit
CCACAGCAGGGAGCACATGGTATCAGTTTGACACATTGTTTGTGATATTA11.282051282051282No Hit
GGTAATAATGCTGCACCTTCTTGAATATAAACTCATGCCACACATGAGTT11.282051282051282No Hit
GTGGAGATTTCAGCCTCTTTGAGGTCAATGGTAGAATAGGAAATATCTTC11.282051282051282No Hit
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCTGTCTC11.282051282051282No Hit
GCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACA11.282051282051282No Hit
GTCTCCCTCTGTCACCAGGCTGAAGTGCAGTGGCACAATCTCAGCTCACT11.282051282051282No Hit
GCCCAGCCCAGCCAGCCCGGCGCGGCGCGCAGCGGCGAATCGAACGCGCC11.282051282051282No Hit
GTAGAAATGAAGAGTGAGTGGAAGGGTCTTCAGATCCTCAGGCTACTCGG11.282051282051282No Hit
CGTCGACATGACGAACCAGCTCCTCGAGGACTCTGCGGCTGATATCGAGG11.282051282051282No Hit
GTCTTAACCTTGCCGTTTTATGAGTCATATAGCCACATTCCAGGTTATGA11.282051282051282No Hit
GGCCTCCGAGGCCTGAAAGCGCCGGAAATCCGGCGCTTGCATCGAGCCCG11.282051282051282No Hit
CTGCATCGTTGCGCTCACGGAGCATTCCCAAAAGCGCAACCTGAAAGGTC11.282051282051282No Hit
GCTCTGTATGGGATATCCCTCCCAAACTTTCTTCATGTCATAGAACCAGG11.282051282051282No Hit
ATTCCATTCCATTCCATTCCGGATGATTCCATTCCATTGCATTCCGTTCC11.282051282051282No Hit
CCCAACAATTGACTGGGCCTGGAAAGCCTCACAGCTCCTTCTCTTTCAAG11.282051282051282No Hit
ATGTACAGATCCCAAAGAGAAGAGGGGTGTCAATGAGGGTTGACAGGAAG11.282051282051282No Hit
GGATGATAAACCGGCCAAACGGCGCGCCGGTCAAGAGACCAGCTGGCCTC11.282051282051282No Hit
GCAAAATATATACACCAAAAGGCTGGGAACACCTCAATGTCCAACAATAT11.282051282051282No Hit
CATCGACCTCGGCATGATCTCGCTCGCCAAGGCCGCCGCCGACCCCCTGT11.282051282051282No Hit
GCCCGGTGACGAGGATGATGCCATTGGGTTCTGCCAGCGCCTCGCGCAAT11.282051282051282No Hit
CTCCCCCGGCACACTTAGTGACAGTGATGCTCTGTGCTGGTACCAAACAA11.282051282051282No Hit
GATATGACTGCAATGATCAAAGACTTAGATTACAACACTCGATTCCCTTT11.282051282051282No Hit
CCCGAGCGCGCCGTGGACGGCACGTTCCTGATGCCCGTGGAAGACGTGTT11.282051282051282No Hit
AAAAGATACGAATGAACAACATATAAAGCAACTGAAGCTTGGACTCACAG11.282051282051282No Hit
ACCAAGGACTTTAGCCAAATGGCCGGGGCCAGGAGTGGGGCAGGGCTCCT11.282051282051282No Hit
AGAAATTCAGTGGGGATAGAATCCTAGAGAATCCTCCAAAATTCACTGAT11.282051282051282No Hit
ATTCCTTTCCTTTCCGTTTCATTAGATTCCATTGCATTCGATTCCATTCA11.282051282051282No Hit
CAATGATTCCATTCGAATCTATTCCATGATGATTCCTTTCGATTCCATTC11.282051282051282No Hit
GGTTTGAAGGCAAAGACATCAAAAATGAAGAAAAGAATTTCCTAATCAGT11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)