FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATGTCATTTGAATTTTCAGCACCAGAATTTTTGCCTGATTCTTCA35.084745762711865No Hit
GGGCTTAAACAATCCTCCTGCCTCAGCTTACCCAGTATCTAGGACTATAG23.389830508474576No Hit
TCCTTACACCTTATACAAAAATCAATTCAAGATGGATTAAAGACTTAAAC23.389830508474576No Hit
GCCGACTTCCAAGGCAAGCACGTGGTGCTGGAATGGACCAACCCCGGCTG23.389830508474576No Hit
AAATGATCTGCCCGAACATTCGGGCCAAATAGCAATTCAACGTCTTGCCC23.389830508474576No Hit
CCCTGAACAGCGCGCTGTTTATCCTGCGCCGTTATGTGGAGTACTACCAC23.389830508474576No Hit
ATGTCCATCACCGCGTGCAGCGTGTGGCCGGAGTCGGCCAGGTCGTCGAC23.389830508474576No Hit
CATTTGAGCAGCTGAATAACCTGCACAGGCTTGCTGTATGACCCCTGGCC11.694915254237288No Hit
CCTGTAATCCCAGCCAGAAAGGGGCAGAGCTAGGACCCGTGCCTGCCCTT11.694915254237288No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGTGGAGGCATCTCGTA11.694915254237288No Hit
GGCGTGGCGGTGGTGCTGAAGGCACCCGGCGCCGACTGGCCGGTGCCGTC11.694915254237288No Hit
CCACCACACCCGGCCAAAACTGAATGCTTTAACAGCACCCAAGTCATATC11.694915254237288No Hit
GCGTGGATGCCACGGCATCGATCAGCGCGGGGTCGATCAGCGGCTCGTCG11.694915254237288No Hit
CTCCTGGACTCAATCATGGCTTGTGGTCTGGTCGCCAGCAACCTGAATCT11.694915254237288No Hit
CCATAGAAGTCAGAGGAGAGATAGTGCCGCCTTTAAACATTGTAAACTTT11.694915254237288No Hit
GCTCATGAATTGCCATCGCCCGGGAGGGGAGTCGGTCTTCATCTATTTCT11.694915254237288No Hit
GTGTGGGAACCATGTGGGACATGCAAAAGAGCATTAGCTAGAGCTACAAG11.694915254237288No Hit
GTGTCGCCGTACATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG11.694915254237288Clontech Universal Primer Mix Long (96% over 26bp)
CATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTC11.694915254237288No Hit
CTGCGGGAGGCCAACGAGATCCTGAAGAAAGCCTCGATTTTCTTCGCGAG11.694915254237288No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC11.694915254237288No Hit
CTACTGCTCCTGGCCTGGTGGACTAGCTTTTTGTTATGCTGCTGGATTCA11.694915254237288No Hit
CGAATGGAATCATCGAATGGAATGGAATGGAACAGTCAATGAACTCGAAT11.694915254237288No Hit
CTTCTGGATGAGAGGTTTATAAAGAAAGGGGTGTTTGGCTACAGAAAAAT11.694915254237288No Hit
CTTCATGGTGTAGAGGCCGCGACGGATGGCGGCGGGCGACTTGTCGAGCA11.694915254237288No Hit
GCAGTATGCGCCCGACATGGCGACCACAGCTGCGCGCGACGATCGCCTGA11.694915254237288No Hit
GAACCACAGGCCAAGCCTGCATTTTTTTAAGACTTCTCTAGAGTTGATTT11.694915254237288No Hit
GTATTAATTCTTCCAGTCCATGAACATGAAATGTGTTTTTTTTTTTTTTG11.694915254237288No Hit
ATCCAGGGGGATCAAGACGAGGTAGAACTTCTGCCTCGATCTTCCAACGA11.694915254237288No Hit
ATAATGACCGTTGTAATAACTGATTTGCATAGAAATCATCAACGGATGCT11.694915254237288No Hit
GGAAACTGCGAGTCAACTATATACTACACACAACAACACTAATCTACATG11.694915254237288No Hit
CACCCCTTGACTCTGTTCTCACTACTGTCACTCCACTGGACGCTGGATAT11.694915254237288No Hit
TCGCCGGATCGAAGCGCTCGGTGCCGTTCAGGCCGAGCGTTCCCATCAGG11.694915254237288No Hit
ATTAAATATAGGATTATAGACATTCATTAGTCTAGCGGTAATAGAAGACT11.694915254237288No Hit
GTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTAC11.694915254237288No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGTGGAGGCATCTCGTATGCCG11.694915254237288No Hit
AGGTGATGGCGCGGCTCGACATCGCCGAACGCCGCGTGCCGCAGGACGGC11.694915254237288No Hit
GTGAATCTTCTATTTGACATTTCTAGAAACCGGAGGAAAATCTAGGGTGT11.694915254237288No Hit
CATTGAAATCGAGTTGATTCCATTCCATTCCATTCCATTCCATTCCATTC11.694915254237288No Hit
CGACAAGCCGCTGATCCAGTACGCCGTCGAAGAGGCGTACGCAGCCGGCA11.694915254237288No Hit
CTATTACACATCAATAAAAAAAAATTAACTCTTTCATATGGTCACCTATA11.694915254237288No Hit
AATATACACCATGCTTAGTGTGGGTCACTGCTCAGTCAGCTTAGCAGATA11.694915254237288No Hit
GGTAATGCTTGCTTACTGGCTGCTCACCTCCTGCTGTGCAGCCCAGTTCC11.694915254237288No Hit
GGTAATAACTATGTCTTATTTTTACATGGTTATTGAGAAGATTAAATTAG11.694915254237288No Hit
ATGCTGGCCTCATAAAATGAGTTAGGGAGGATTCCCTCTTTTTTATCGAT11.694915254237288No Hit
CACGAAAGCGGTGCGCTGACGGGTGCCTTCGGCAACCTGCCCGGCCGCTT11.694915254237288No Hit
ATAACAGGCACAGAGCTGTCGTCTCCTACGACGACGATGGCTTGTTCTAG11.694915254237288No Hit
GTTATTCGTTGTCTTCTGCTGGCTTTTGAATTTGTTTGCTCTTGCTTTTC11.694915254237288No Hit
ATTACATTTATATTTCTACTAATTTATTCTCTACAGGAAAACATTCATGA11.694915254237288No Hit
GAACCACGCAGGCGGCCTTCGACCCGTACGCGGTCGACGTGAGCTTCAAC11.694915254237288No Hit
ATCATATTGTGTGTGTTTCATGCTGTATTTCATTTCCTGCAGAGTTTGTG11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)