FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length146
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTCCTTTTAAAGATACCTAAATTAGATTTTCCTTACAAGTAAATTCC46.153846153846154No Hit
AATGGGGACTATAGCTCATCTTGCTAAACTTCTTCTTGAAATTTTCTCTC34.615384615384616No Hit
CAATATAATGGAATGGCATCCAATGGCAAGGAATGGAGCGGACTCCAATG23.076923076923077No Hit
CTGTTTATAGAACCTTTTCTCAGTTCTCCTGTTTTCAGCCTCAGTCCAAA23.076923076923077No Hit
TCCATATGTTGGGTTACAAGAAAAGCACCTAGTTTATGATATACGTAAAT23.076923076923077No Hit
GGGAAGTTGGGGTGAAGCCCGGATGTGTCTGAGCCAGATGCCAGCTTTGC23.076923076923077No Hit
GAATTGTGCCCGTTTCTGAGACATTAGTAAGTCTATAGAGCAGATTTAGC23.076923076923077No Hit
ATTATCTCTGTCTTGCAGACAAGGAAATGCAGGCACAGAGAGTTTAGCAA23.076923076923077No Hit
GGTTGAACTAATTTACACTCCCACCAACCGTGTAAAAGCATTCCAGTTTC11.5384615384615385No Hit
GGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGA11.5384615384615385No Hit
GGGATTCATGGTTAATTGATGATAAGGTTACATTTTCTAAAAATATGGAC11.5384615384615385No Hit
ATTTATTTACCTGAATCGCTTGCTGCAACAACTGTTCCACCAGGAGCAAA11.5384615384615385No Hit
ACCAACATCTTCTCAACCCCCTGAATCCCCATCTCCTAGAAACCATGATT11.5384615384615385No Hit
CTTCCTTACCAGTAAAATTTGACACCTCAAAGAATGATTAGTATAATTTG11.5384615384615385No Hit
GTGTTCTCTCATCTGTAGGCCTTGTTTATGTTTCGCTGATAGTCTAGTGA11.5384615384615385No Hit
GCATTCAAGAGGTGACTTGGGTGCTGTTAAAGGCATTCCATTTTAAAAAG11.5384615384615385No Hit
GCTGAGAAGGCTGCACCAAAGAGAGGGGACCTGGGTTGGACCTTGGTGCA11.5384615384615385No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA11.5384615384615385No Hit
GTTCAGGAATCGAAAGAAATAGGTATAAACTCAAAAGTTAACTCCCTAAT11.5384615384615385No Hit
TACTTGCCCCACCCCTGCTGGAGTTACCCAGTTACTCTCATGAGCCACAG11.5384615384615385No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG11.5384615384615385No Hit
ATTCTGTTCCATTCCAATCTGGTGGAATCCATTTTATTCCATTCCACTCC11.5384615384615385No Hit
GTTGAGGGCAGTGGATCTGAGTGCAGTGGAATGAAATGGAATGGAAAGGA11.5384615384615385No Hit
ATCCGCCCACCTTGGCCTTACAAAGTGCTGGGATTCAGGCATGAGCCACC11.5384615384615385No Hit
CAGTTAAGCACTGAAGTGGGGAAGGGGCACTACTATGGAAAATAGAAAAA11.5384615384615385No Hit
GACGGGGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCA11.5384615384615385No Hit
TGTGTTGATTTCTAACACAAACCCTTACTTTTCTGGGAAGATGTTTAGAT11.5384615384615385No Hit
TCCCAGCACTTTGGGAGGCTAGGCGGGCGGATCACCCGAGATCAGGAGTT11.5384615384615385No Hit
CATAAGGTCTGATCACATTTAAAGTCAGTTTATCCCGAAGATCTAAGGAC11.5384615384615385No Hit
CTTGCGGCCCGTCAGGCCCTGCATGGCTTTCTGGCAGTCAAACACAGAGG11.5384615384615385No Hit
GGTTATCTGTCCTCATCTCCCCTGTTTTGTCACCACTGAATCACTCCCTG11.5384615384615385No Hit
TGGCGCCTGTGGGGCCAACTGGGCCTGCGCGACGCTGTGACCCTGGAGCT11.5384615384615385No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG11.5384615384615385No Hit
ATCAATGAGACAGAAAGTTAACAAGGATATCCAGGAATTGAACTCAGCTC11.5384615384615385No Hit
GGTAAAATCTGAGAATGACTCACAGAAGCATGCCAAAGGGGCTTCTGGCC11.5384615384615385No Hit
CTGAAATCCATTTTATCCTCATTTTTTTCTATATCTTCCTGAATTCTCAT11.5384615384615385No Hit
CAGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTG11.5384615384615385No Hit
AGGTAGAACCGTGCATCTTAAAAAAGCTTTTCAGGAAATTCTGATGGGCA11.5384615384615385No Hit
TGCCTCAGCCTCCTTTTAAAGTGCTGGGATTACAGACGTCAGCCATCCTG11.5384615384615385No Hit
GTTTCAAACCTGCTCTCTCAAAGGGAATGTTCAACTCTGTGACTTCAATG11.5384615384615385No Hit
GAGTGTGGAGTTAGTTTTATTTTTAGACTCAGGTCAAAGGGGCCCCAGCT11.5384615384615385No Hit
CTTCCTTACCAGTAAAATTTGACACCTCAAAGAATGATTAGTATACTTTG11.5384615384615385No Hit
GCAGCAAATCCCGCATATCTTCTGGGAACTACAGGAAATAGCTGGGAAAG11.5384615384615385No Hit
AAGTAAAGAAAGGCATGTCATGCCAAACACCTAAAGCATTTCAATTTCCC11.5384615384615385No Hit
GCCTGGAGCGATGACGGCAAACTCCTCTCCGCCATACCGGAAGGCGATAT11.5384615384615385No Hit
GTACAGGCCGAGCAACGCCATCATGATGATCGGCATGCGCTGCGCCCAGC11.5384615384615385No Hit
CCAATAGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACC11.5384615384615385No Hit
TTCCAATCCATTTCTTTCGAGTCCATTCCTTTCCATTCAATTCCTTTCCA11.5384615384615385No Hit
TTCCTATCCCGACAACGGCGGGTTCGAGGTGATCTCGAACATGCTGCCCG11.5384615384615385No Hit
GTTTTAGGGTGTCTACCTTTGTTATCTATGAGTGCATAACAAATTAGCCA11.5384615384615385No Hit
CTCCTGGAGCTGTCTGGTAGTGTTCATTACACCACTCCAAAGCCTCAAGT11.5384615384615385No Hit
CCAACACACTTAGCATACAAAGGAAGAACCCAGTAAATAACAGTGATCTA11.5384615384615385No Hit
CATAACACATGTATGATTACACAGACAGAGAGAAGAAGATCCAGTAGTTA11.5384615384615385No Hit
GAGAGGTCTGGTCTCGCTCTTCTTATAAGGACACTAATCCTATCAGACTT11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)