FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length146
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTCCCTGATGGGGTTCTTCTCACTGCTCCTAGTGCCTGTGATGCTC46.153846153846154No Hit
GATCTCATTGGGCACTCCGGAATAGAAATCTACAACTCAAGGTTTGCCCC34.615384615384616No Hit
ACACAAGGACAAAGGGATCAGAAAAAGCAACAATAGTGAACAAGAGATAA23.076923076923077No Hit
CCCTCAGTGCTTCCCCTGTGCTCCCTGAGTATCGGGCACATCCCTAGATC23.076923076923077No Hit
AAACAGAACACTCCCATTAGGAGATCTTCCACTGCTGATGACTTTAAAGC23.076923076923077No Hit
CTATTCAAACATGTACCTTCTCCAGGTCTTTCATCTAAAATGTATATGTA23.076923076923077No Hit
AGTATAGAAATACCTTAAAGGTACTGTTCTTCCCGTGAGCCCTAAAGGAA23.076923076923077No Hit
GATTACAGGTGTGAGCCACTGCACCCAGCAAGCCTGGGCTCTCAACCAAC23.076923076923077No Hit
TTTCTGTTCCATCTGATTCCATTCCTTTCCATTCCATTCAATGCCATTCC23.076923076923077No Hit
CTTCAGCGCGGACTTCGCGAGCAGTCGATCCGCGATCCGCTCACCAACCT11.5384615384615385No Hit
CCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCGAGGCGCCCGCCGAGGCG11.5384615384615385No Hit
GCCTTTTCAAAATAATGCTTTTTAAACACTTTTTCCTTTAAAGTAATTCA11.5384615384615385No Hit
CCCAGGTGCAGATGGGCGGCCACCCGACTGAGGTCCTGTGCCTCATGAAC11.5384615384615385No Hit
CTTTTGGGTTAATGCTGAAATGAGTTAAGACTTTGGGGGACTGTTGGGAA11.5384615384615385No Hit
ACCCAGGAGGTTCCTTGGCAGCCAGGGCTTTGAGATCTTGCCCCTAGTGA11.5384615384615385No Hit
GGGCGGATCACTTCACGCCAGGAGTTCAAGACTAGCCTGGCCAACATAGC11.5384615384615385No Hit
ATGGAGGCAGGGTCTTCCTCTGTTTTGTTCACCTGTGTCCCCAAGGACTT11.5384615384615385No Hit
GCAGTGGAAGGAGGCCCGTGAAGATACTAGAAGTGGGAACAAGGGCATTT11.5384615384615385No Hit
GTCGAGGATGCGCAGCGGGTTGGTCTTGAGGCGGCGCAGGCTGTCTTCGT11.5384615384615385No Hit
ACTCCACTCCACTCCACTCCACTGCTTTCCATTCCACTCGACTCCCCTCA11.5384615384615385No Hit
TTCCACTGCATTCCAGCCTGGATGACAAGAGCGAAACTCCGTCTCAAAAA11.5384615384615385No Hit
ACATGGGCCTGGGCACTTGGCTGGCCACCCAGCCGTGGATGGTGAACGTG11.5384615384615385No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC11.5384615384615385No Hit
CTCCACCTCCCAGGTTCAAGTGATTCTTCTGCCTCAGCCTCCCAAGTAGC11.5384615384615385No Hit
ACCACCACCCTACCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTC11.5384615384615385No Hit
CTTATCCAAGGAGTAAAATAGAAACCACATCCACACTCAAGCGAATTCAG11.5384615384615385No Hit
AAACAACCCCATCAAAAACTGGGCAAAGGACGTGAACAGACACTTCTCAA11.5384615384615385No Hit
GTATCAACGCAGAGTGGAATCGAGTGGATTGGAATCGAGTGGAATGGAAT11.5384615384615385No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG11.5384615384615385No Hit
GATACTATGTGATAGCTATTTGCCAAAGATGGCCTCTAGTATCCATGCTT11.5384615384615385No Hit
AAATAAGTTAGCTGCAGATTCTTGGGTTGTGGAGTGAGTGTTCAAGTCTT11.5384615384615385No Hit
CCACCACCATCATTGCCATCACCACCACCATTATAACCACCACCACCATC11.5384615384615385No Hit
GTGTTCTGAGAGACAGTTTGTTATAATTTCTTTCTTTTACATTTGCTGAG11.5384615384615385No Hit
ATATAAGTTTGCTCAAAGTTGGATGAAGAGTTATCTAATTTTTCACATGC11.5384615384615385No Hit
AGCTAACAGAGTTGAACCTTTCTTTTGAGAGAGCAGTTTTGCAACACTCT11.5384615384615385No Hit
CATTTCAGCTGCATGGAAAGCTGAGGCAGGAGGATTGCTTGAGCCTGCTC11.5384615384615385No Hit
GCGCCGGCAAGCCCTTCGCCTCCGCCTCTTCCTCGGGCAGCATGTTCGAG11.5384615384615385No Hit
GGCTCATTGAAACCTGTGCCTCCTGGGTTCAAACGATTCTCCTGCCTCAG11.5384615384615385No Hit
GATAGGGGGAGAAAGTAAAGGAAATATCCGCATGAATCAGAGAGCTTCCT11.5384615384615385No Hit
TTGCTAGATGTTTTAATCTAAGACCAGTTCTGTATGGAACATCAATTTAG11.5384615384615385No Hit
GCAAGTATCTAAGCGTGAAGTCTTTGTTCTTGCTTGGCCAATTAAAAGCA11.5384615384615385No Hit
CTATGGGGAAACATTTGTTCCTAATGTCTATATTCCTTAATTAATTAAGA11.5384615384615385No Hit
TATTCATGAACAGCGGAAGGACTTGGGCTTTTAAGGAGTTACTCACAAAA11.5384615384615385No Hit
GAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTC11.5384615384615385No Hit
GGAAATGAATGGAAGGTAATGGGAAGATATCGAACGGATTGGAAAGGAAC11.5384615384615385No Hit
TTATTAAGATGAACCTTTTCAAAGCTTCTAGCTCCCTAACCTCTATTCTG11.5384615384615385No Hit
CCTGTGAAGTGCAGCATTGTTTGTAAAGCGTCTCTTTCCTCTGATAAACA11.5384615384615385No Hit
GAGCCGAGATCACACCACTGCATTCCAGCCTGGGCAACAGAGTGAGACTC11.5384615384615385No Hit
GGCCGCCAAGCCCAGCGGCATCACTGGAATCCAATCACGAGGAAACATGC11.5384615384615385No Hit
CTATGATATGAAATGCCTTACCTGGAATTAGCCTACATAGCAGTTGGAAA11.5384615384615385No Hit
CCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA11.5384615384615385No Hit
GTTGTCTTCCATTTCCTAACCTAGCATTAGCCTCTCCTCCACTTTCCCTC11.5384615384615385No Hit
GTTGACTGAATTATTTTTTTCCATTTTGTCTTGCCATTCTTTTTGCACAC11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)