FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length146
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAGTGGGGTGGAGGTTGCAGTGAGCCGAGATCACACCACTGCACTCC22.272727272727273No Hit
ACCCCGCCTCTACTAAAAATACAAAATTAGTTGGGCGTTGTGGTGCATGC22.272727272727273No Hit
CCGGTGCACCACGAGCCTGCCCTCCACACCAAGCCGGCAGCACCTGCCGA22.272727272727273No Hit
CTGCTGTACTGGACAATGTAGATGCAGCACTAAGAAAGAGCAAGGCTCTC22.272727272727273No Hit
GACGAGCACGGCATCATTCCCGAGGCCCTGGTGGAGACGATCCGCGACGT22.272727272727273No Hit
GTCGAAGGAATGGTCGGGCGGGGCGGATGAAGCGGCCTCCAGTCTACGGT22.272727272727273No Hit
GTACCGAGGCAAAAGGTCGGAAGTGTCCTGTTTGCGGACATGAGACGCTG22.272727272727273No Hit
GAGATACACTGCATTAGTACATGTACGACGCCGGCCGTGGGGTCGGCAGA22.272727272727273No Hit
GTCGAAGAAAGGAACCGGCTGGTTTTCTGCTGCCGTCTCCCTTGCCCTCA22.272727272727273No Hit
GCGCTGGCTCCCTGTCATGCCTTCTTCCAGTGCTACGCGGCCCGCGGCCG22.272727272727273No Hit
GGCCACAGCATCCTGAGCAGGCACCACACCTGTCCCAAGCCTCACCAGCA22.272727272727273No Hit
GGAGCTGTGCGAGCACGACGCCCGACGTACCTCGCGCGCCGAGCACCGCC22.272727272727273No Hit
GCTAAGAGGCCTGGCTGATTGGAAGAGGATACGAAGTCAGAAAACCCTGC22.272727272727273No Hit
GACCGATGAAGACCTCTTCTCCCGCGTCTACCCCATGGACCTGCGCCCCC11.1363636363636365No Hit
CGGTGACGTCGAGTGCGCGGTCTTAGCGCCACTCGCCACCACCGAGTGGC11.1363636363636365No Hit
TCTCGGAAGTGACATCCCATCACATAGGCTATAATCCATCTGTCTCTTAT11.1363636363636365No Hit
GACAGAAACACGACCAGCACCTGCCAGGAGGTCACCCATGGGCGCTACAC11.1363636363636365No Hit
GGCATACCATCCACGCTGTTGACGTCACAGATTAAGCGGGCAATCTTGGT11.1363636363636365No Hit
GTACTCGCCTGATGGTGCGTGCTCGCGCGCAGGACCCGAAGACTGGTTTG11.1363636363636365No Hit
TCTCCCAGATGGACGACGAGCTCTTCCGCGAGCGCGCGACCGATGTGGCC11.1363636363636365No Hit
GAGGAATCCCGTCAATCGGCGTGCCGTCCATCGTAACTGCGCCCTCCTGT11.1363636363636365No Hit
GCCAAACACCATTCTACCCTCTGCCTCTGAGACAGCAAGATAGAGCTTAA11.1363636363636365No Hit
GGTCGATGTCGCAGTAGGCGACGGCGTCGCCGCCAACCTCCGGCAGAGAA11.1363636363636365No Hit
ACCCAGAGTCGGGTATAAAGCTCAAACTATGAACAGCCACTGTCTCTTAT11.1363636363636365No Hit
GTCTGTCACACTGACCCTCCACTGAGCTGCTAACATTTAAGCTGTCCATG11.1363636363636365No Hit
GCGCCGCTCGCGCGCCGGGGTGTCCGACCCGGACCGCCCCACCGGATCCT11.1363636363636365No Hit
CTCGTCGACTTACTCAGCCACCTCGAGCCCGGCGAGATCGACACTGTCGT11.1363636363636365No Hit
GTGGGCTACCCGAGTCTGCATGTGCTGGCCCGTGCTCTGGGTCGTAACCG11.1363636363636365No Hit
ATCCTCCTTGGGTAGCCCTCCAAGAACGTCGCCCATGTAGGCATAAGTAA11.1363636363636365No Hit
TTGACGGGCGACAGGTGCCAGTCGGTCGTGGGCGTGTCGCCGGCCCATGA11.1363636363636365No Hit
ACACCTGGCTACGCAACGGTCAGCAGGTACGCGCCGCATCGGGCGGCGAA11.1363636363636365No Hit
GCCTCCACAGGGTCGGGACTTGGGGAACACTTGCTGTTTAAATGATGGAC11.1363636363636365No Hit
AAGGAGATCCTCCTCCGGAAGGGCGTGCAGCTGCTGCTGAGTACGTGCAC11.1363636363636365No Hit
GGAGTGGACGGACAGCAGCGCCTCAACAGGAGCCTCAACCCTGACGTCCG11.1363636363636365No Hit
TCGTAGTGTGCGTTTCAGGTTCGCCCGCGTCAATAATCTCGGCGGCACCA11.1363636363636365No Hit
AGCCGCTGCCGTTCGTGCGGCCGCCGTAGATGTTGCCGCCCACGCTGCCG11.1363636363636365No Hit
AAGTGGAGGGATTTGGAGAGCAGACCACCAGAAAAGGCCAGGTTAGACAG11.1363636363636365No Hit
TTCTTCTCCTCTGTCTTCTGCGTCCGTGGCCCTGGACTGACTGTGTGGAG11.1363636363636365No Hit
CTACGGATTCGTTCGAGCGCGGCGCGTCATCATCATGGGTTTCGTGGCTT11.1363636363636365No Hit
CATTGATGCTGATTCGACCGTGCGCCCGCTCGATTCGGTCGTGTGGACCA11.1363636363636365No Hit
GCCCGGCCCACATGGCAAAACCCCATCTCTACTAAAAATGCAATAATTAG11.1363636363636365No Hit
CATGCGCTCCCTCTTCTCACTGTTGACGTTCATGATCCCGAGAAGAGTCA11.1363636363636365No Hit
CAACAACATCATCTCGATCGCCTCGATCCTGGCCTACCTCCATATCTACG11.1363636363636365No Hit
CTTGACTCCCAGACCAGTGCTTTTCCCTTTGTTTTCTTTCCTGTGCTCCT11.1363636363636365No Hit
AGGGCCAACCAGCTGCGGAGCCGCCCCGACGTGCGCAGGGGCCTGGACCT11.1363636363636365No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATC11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATCACCTGCGAGGATGGGGCCATGACGACCGGCGCGCTGGACCTTCCCG11.1363636363636365No Hit
CGCCATCACCGACTGCTCGGTGCCTCTGCCGAGCACCTCGGGCGCGTAGA11.1363636363636365No Hit
GTACGCGCACCTGTACACCGCGTTTGTGGGCGGCGAAGAGGGCGTCCCAG11.1363636363636365No Hit
CCAGACCCTCATGGACTCCTGCCTGCGCCACGGCGTCGGCGCCAGCGTGG11.1363636363636365No Hit
ATGGGATGCTTTTCCTGTAGCTTGACCAGAATTTCTTCGTTTGTAGAGTA11.1363636363636365No Hit
CGCTGGTACGGAGCGTCGCTCACGTACAGTTCCTCAAAGGTGCGCCAGGT11.1363636363636365No Hit
AACCCAAGCCTCTGTCACTAAGGTCCATGCTCCTGCCCACCCCAGACTGC11.1363636363636365No Hit
GGCAACATTAACACCACCAGCCCAGGACTACCAGGTCTGTCTCTTATACA11.1363636363636365No Hit
GTCACGGAAACGGTGGCTGTCGATGGTCAGTCTGCTGGTCACGCCGGCCG11.1363636363636365No Hit
GGTGACTGGTGAGTACACTAGTGCACAGAGGGCCATCAACTCGATTTTCG11.1363636363636365No Hit
ATCAAGCGCTTCTTCACCCCCGCCTCGAAGAACAAGGACGGCAAGTACGG11.1363636363636365No Hit
CTACATACCTGCCATCCGCGGGCCACTGCGGAGAAGTGTTCGGCGGGCTT11.1363636363636365No Hit
GCCGTCACAGCATTCGACGCCGCCATCGCATTACGCTCAATACACGGAAT11.1363636363636365No Hit
TGCCCATCGTGCTGTTCCAGAATGCGCAGGCAAAAGCGGAAAGCAGGGAT11.1363636363636365No Hit
GCCTTTGCCATCGCAGCCCACTGAGCTTCCCACCACGGAGGGGCCCCAAG11.1363636363636365No Hit
ATCGTGTTGGTCGCGCCTGCGCGATCCCGCTCGGGATGCAGGTGCGCGAA11.1363636363636365No Hit
ATGCTGGGCCCCGACAGCCACATGCACGATCTTCTTCCCACTCAGCCCTT11.1363636363636365No Hit
GGCTGATGCTCTTGACTTCTACCGAAGCCACTGACCGAGCGAGGAGCCTC11.1363636363636365No Hit
GTGTGGTGGCACGTGCCTGTAATCTCAGCTACTTGGGAGCCTGAGGCAGG11.1363636363636365No Hit
CGATCCGGCCGGCCGAGCCGTCCGACTCCTGCGACAGGGCGGCCCGCGCG11.1363636363636365No Hit
GTACATCACCATCATCAAACAGAGCAGAAAAATTGGAAACTCTAAAAAGC11.1363636363636365No Hit
GTCCTCCCCACCCCATCCTGCAAGCCCCAGAGTTCAAACCCTGCGTCAAG11.1363636363636365No Hit
ATTCTCCTGCCTCAGCCTCCCTAGTAGCTGGGACTACAGATGCATGTCAC11.1363636363636365No Hit
GCTCTAGTGTAACTTCTTTCATGCTCTTCCTTGACCCCTCTGCCTTGGGC11.1363636363636365No Hit
CGTGCAGGACTGAGGCTGGGATCCCGTGCTGCTTCCCCCGTCTCCGCCCT11.1363636363636365No Hit
CCCAACGCCCCGGCCATCGAGGTCGAGTCCTCCACGCGGCGCGGCGCCGC11.1363636363636365No Hit
GCTTACTACACGAAGAAAGACGAATTCAAGGTCGACATCATTCCATTTGA11.1363636363636365No Hit
CTCCCAGCCCCACCTCTGCCTCCCAGGTCTGAAGAGGAAGATGAGGTAAC11.1363636363636365No Hit
CGACGACATCGACCCGCTCATGGCCCCCGAGCCGCTCACGATCATGGGGC11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)