FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length146
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGCAGCGTGTACTCGCGGGCAAGCTCCTTTCCCTCGGCCTTTGGCA22.272727272727273No Hit
GCCCCTCGTACTCAGCGGGGCCGGGGACGGTGCCCTTACCCGCTGCGCCC22.272727272727273No Hit
GCCAATTACACCATACCCCACTGCTGGTGCGCTGCGAAACGCGAGGCGTC22.272727272727273No Hit
CTGTCACCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCT22.272727272727273No Hit
AGATGAACTTGATCGTGCGGCCCGAAGCGCGCACGTCGCGGATCGTCTCC22.272727272727273No Hit
GCAGGTTACGTAAGAAAATATAACTTGATTGTATCTTCATAAAGGTTCTT22.272727272727273No Hit
GCCATGGCTGATGCCTGAGCATCACTTTGGCTTCCCGTAGCAGGGTTTTC22.272727272727273No Hit
ACAATTGCTCGCGCACCTGGGCGACGTGGTTGTCGTAGATGTGGCAGTCG22.272727272727273No Hit
ATAAAGCCACCACGCAAACGTCAACGCATTGATGACGACGCCGGTCACGG22.272727272727273No Hit
CTTTCAAGAAAGAGGCAGGGGGAGCGCTCAGCGCTTCCGGGTACTCATGT22.272727272727273No Hit
GAGTCTTGCCCAGGGCCCTGCCACCAGCTACTGAGAGCCTTGCTCTTTCT22.272727272727273No Hit
GTGCAGTGGCGCCCTCTTGGCTCACTGCAACCTCCGCCTCCCTCCCTGGC22.272727272727273No Hit
CTTCTTTTCTGAAACAAGGCACCAGCTAGCCCAGTGCTGGTGAGGCTTGG22.272727272727273No Hit
CTCCGTGACGATGCCGGCAACGTTCTCCTTGTCGATGTCGGCCGTACTCG11.1363636363636365No Hit
CCCATGGGGGTGACGACGTTGCCCTTGGACTTGCTCATCTTCTTGTGCTC11.1363636363636365No Hit
CACGAGCACGTAGGAGTTGAGGAGCTGCCCCGCGAGGTATCCGACCAGCG11.1363636363636365No Hit
GGCGGCACGGTCACGGGAGACGTCTACGGCGGTCAGATCGTCGACACAGG11.1363636363636365No Hit
GGCGTGAACGGCTACCTCTTCGAACCCAATAACGCTCAGGATTTGGCGAA11.1363636363636365No Hit
GTTCTGATCCGACAGGATGTTGAAGTGGTCGGACGCCTGGTAGAGCGGGA11.1363636363636365No Hit
ACCTGGTAGTCCTGGGCTGGTGGTGTTAATGTTGCCCTGTCTCTTATACA11.1363636363636365No Hit
CCTCGCTGCTCGCCGAAGTGACGGGGACTCCATCCTCGATGAGGCCGTCG11.1363636363636365No Hit
GCCGGGTACAGGGCTCTGCCCCCACGGACACGAAGGCCGCCACCCTCTGT11.1363636363636365No Hit
TGATTATCTATCCCTGCTTTCCGCTTTTGCCTGCGCATTCTGGAACAGCA11.1363636363636365No Hit
TCTCGGATCACTGCAACCTCCACCTCCCAAGTTCAAGCAATTCTCCTGCC11.1363636363636365No Hit
GCCTCCACAGGGGTCTCAAACAGGTTGACATGCACCGCGTTTTTGTTCCA11.1363636363636365No Hit
GGGAGGCACACGCATCCCTCGGCGTCCACGACCCGTGAGCAGATCTCGCC11.1363636363636365No Hit
GGCGCAGGTGATGGGTGGTACTCCTGAGCAGGTTGAGAATGCTGCTGAGA11.1363636363636365No Hit
CATCCCAATATCCGGCTGCTGCAACTCGATAAAGTTACTGCCGCTAAGCC11.1363636363636365No Hit
CTCTGATTCCCTGACTCTCCACACAGCCAGGAGGGAGCCGGAGAAGCCAC11.1363636363636365No Hit
GACCTGGAACGAGTGCCTGACTCTTCTCGGGATCATGAACGTCAACAGTG11.1363636363636365No Hit
GTGGAGCGATCGGCGTCTTGTCGTTCTCTCCGGGGCGTTGGACCCCTGTT11.1363636363636365No Hit
TCCCTGCACCTCTCTGGTTCTTAGTCTCCCCCAACAAGAAGCATAAAGGC11.1363636363636365No Hit
CTACTTATGATCAGGACACGTCACAGTGCAGCCTTCACTTGAGGACTTAG11.1363636363636365No Hit
GTAGCCAGCCAACTCAACTACACCCTCGGCTCGCTCCCCCTCGTCGGCGG11.1363636363636365No Hit
AGGCTGTTCATAGTTTGAGCGTTATACCCGACTCTGTGTCTGTCTCATAT11.1363636363636365No Hit
CTGCAAATTACCGAAAAGCCCAAAAAGACGAAGTCCGGCAGCTACTCTAC11.1363636363636365No Hit
GTGGCAAGGGGTGGGACGAGGACATCGACCCGGCTCTGGCCTCGGTGCCC11.1363636363636365No Hit
CACCTGTGGCCCAAGGCAGAGGGGTCAAGGAAGAGCATGAAAGAAGTTAC11.1363636363636365No Hit
TTGTAGCCGGGCCAGGGCAGGCCGCTCTGCTGGGTCTGCGGCAGGCCATA11.1363636363636365No Hit
CCACCGGCGGCACGTTCAGCGGAACGGACACCTCCGGCGTGGCAGGAGTC11.1363636363636365No Hit
ACGGCGTACCGCATCAAGATTCAGAGCATCCACAGGAGCCACCAGAGCCG11.1363636363636365No Hit
CCTGTCTAACCTGGCCTTTTCTGGTGGTCTGCTCTCCAAATCCCTCCACT11.1363636363636365No Hit
CCGGAATCCGGAGCGCACCAGAGGGATGTAGAGAATCAAGGCCTGAACGG11.1363636363636365No Hit
GGTTGCTACTTAAGCTCTATCTTGCTGTCTCAGAGGCAGAGGGTAGAATG11.1363636363636365No Hit
GGCGGAGACGGGGGAAGCAGCACGGGATCCCAGCCTCAGTCCTGCACGCT11.1363636363636365No Hit
AAGGTGAATCCGGTGACGACAGTGTCGATCTCGCCGGGCTCGAGGTGGCT11.1363636363636365No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATC11.1363636363636365Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCCTGCTACCGTCCGGCGTCGCCCACCTTTTTGATGACTCTTGAACCGC11.1363636363636365No Hit
GACCGCTACTTCGACTACGAGGCCGGCGAGCTCGGCTGGCGCACCCTCGA11.1363636363636365No Hit
GTCTACTCCAACGACCTGGACCCCGACGACGACTACGAAGAAGTCGTCGA11.1363636363636365No Hit
CTCTTCATGAAGCTGCTCTTCGGCAGCGGCGGGAATTTTGGGGGTTCGCG11.1363636363636365No Hit
CGTTGCGGGTGCGCTATCCATCCTCCGGGTGCGCCGATTGGCCTCGGACC11.1363636363636365No Hit
GGTTGGAGGCGAGCCGCAGGATCTCCTCAACGGTGTAGATGCGGAACACG11.1363636363636365No Hit
CCTCCAGCCCATGCTGCCCTCTTCCATTCCTAACTCCTCCCCAAAGCTCT11.1363636363636365No Hit
GTGCACGTACTCAGCAGCAGCTGCACGCCCTTCCGGAGGAGGATCTCCTT11.1363636363636365No Hit
CGGTCATACAGGTGTCGTGTCGTTGTTTTGTGACAATGGCTTCTTTCCAG11.1363636363636365No Hit
GCATGGAGAAGGCCGTCGTGGCCGCAACCAGCGCGAGGAGGAAGACCGCG11.1363636363636365No Hit
GGATTGAAGAGAGCGACATGTACTTGGTACCTGACTATAACACTTGGCTA11.1363636363636365No Hit
CAAAACCCTATTCAAAGTTCTATCCTTGGTTCAATCATTACGAACTAAAG11.1363636363636365No Hit
ATCTTGGCTAACATCATGAAACCCTGTCTCTACTAAAAATGCAAAAAATT11.1363636363636365No Hit
GAGGGGTGTCACGGCTGGCGGTGAGCATGTCGGTACGCGGAACCATGAGC11.1363636363636365No Hit
AAGCAATTCTCCTGCCTCAGGCTCCCAAGTAGCTGAGATTACAGGCACGT11.1363636363636365No Hit
GCCGCAGGTGGTGGAAGGGCTGAGAGGGAAGAAGATCGTGCATGTGGCTG11.1363636363636365No Hit
GTCTGAAGCCTTCTTCTCTCAGCTCGTCAAAGTCATTCTCCATCCAGCTT11.1363636363636365No Hit
GGTGAGGAGGTAGCGCTGGCCGTTGATGTCGACGATCGGGTGTGCCGGGG11.1363636363636365No Hit
ATTCGTCTCCAGGAGACGATCGAGCCGTGGGCCCTGACCGTCGGCCAGAC11.1363636363636365No Hit
GTCCATCATTTAAACAGCAAGTGTTCCCCAAGTCCCGACCCTGTGGAGGC11.1363636363636365No Hit
GCAGGTGACTGTGTGCAGGAGCTGGGACGAGCACCTTTGGGCACCAGCAA11.1363636363636365No Hit
GTTGTCAGAGTGGGTCCTGGCACACTGCAGGTGCTTTATGATGGATGGAT11.1363636363636365No Hit
GAACAGGAAGTCCGCCAGGGCCTTGGCCAGCTCGGTCTTGCCCACGCCGG11.1363636363636365No Hit
CCCTCCTCCTGGATACCGGTCAGGTCCACGCTGGCGCCGACGCCGTGGCG11.1363636363636365No Hit
GGGTGGACATCACCCATACGAACCCGGACATCGCCGACTACGTCGACATC11.1363636363636365No Hit
ATGGATTATAGCCTATGTGATGGGATGTCACTTCCGAGACTGTCTCTTAT11.1363636363636365No Hit
GCCCCCCTCCCCTCGGGGGAGGGGATCATCGGGTAGACGTGGTTGAGGTT11.1363636363636365No Hit
ATCGTGCGCATTGTCGCGGCAGGGAATCTCGTGCGCGGCGATGAGATTCG11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)