FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGATCGAAAGCGGAGAAAAAGAGGTGTTGACAGCAGCGTGTAACGCT44.545454545454546No Hit
TTTATAATGTCTATACATGTGATGTATATAAAATTAAAGCATAAATACTA33.4090909090909087No Hit
GACCTGGAGCGAGCGAAGCGGAAGCTGGAGGGCGACCTGAAGCTGACCCA33.4090909090909087No Hit
GTGTAGGGAAAGAGTGTAAATCTCGGTGGTCGCCGTATCATTCCCATGTA22.272727272727273Illumina Single End PCR Primer 1 (97% over 42bp)
CTTCCAGCGTGAGGCCGCGCGCGTACACCGAGCGCTCCAGCATGCGGGGC22.272727272727273No Hit
CTTCCAAGCTCTGTGAGGTAGGGAACACATTGGAGCATCTGCCTACTCAG22.272727272727273No Hit
GGCATGGGGGGGCCACTAGATGCACCTGGGAGCCCCCTGGCTTGCACTGA22.272727272727273No Hit
CCTCAATGCAACCATTAAACTTAGGAAATTAATATTTGATACACTACTGA22.272727272727273No Hit
GACTCGCATGGGTTGATGATGTGTCTCATTCAGACAGTTACAAAAGCACC22.272727272727273No Hit
AACCTGTTCTTCTCGGCGACGATGCCGAAGGAAATCGCGCATCTGGCCGA22.272727272727273No Hit
GACCACGAACCCACTGGAAGGAAGAAACTCCAGACACATCTGAACATGTG22.272727272727273No Hit
CCACAAGCCGTGTGTTCAAAACCCGCAAGCGTCACTACCGGGTCGGCGAG22.272727272727273No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA22.272727272727273No Hit
CCTCAAGTGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGC22.272727272727273No Hit
CAACGCAGAGTGCAATCTAATGGAATCGAATGGAATGCAATGCAATGGAA22.272727272727273No Hit
GGTCTCGCGCTCCGGCACGCTGACCTACGAAGCCGTGGCTCAGCTGACCG11.1363636363636365No Hit
TTGATGTATGTGATTTATTTCTATTCTTTGTAACCTTAAATAGCCTTCAG11.1363636363636365No Hit
CCTCAAGACCGTGCGCTGACCGAAGCGCGCGGCCGGTTCCGTTTTTCCTT11.1363636363636365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGACAG11.1363636363636365No Hit
AACTACTACAGTTTAAAGCACAAAAAATGTTGATGTTTTTCTTAAATTAT11.1363636363636365No Hit
GAGTGATTTAGATCTTGTAGGATGGAAATATCAAAAGTGCCATTTTCTGG11.1363636363636365No Hit
CCGAAATCCTGCCCTCGCGCGCCGGCGAGCCCGAGCGCATGACGGTCTGG11.1363636363636365No Hit
AGTTAAAAAAAAAAAAAAAAAAAAGATTTTTATGTTGGTTTGCCAAAGGG11.1363636363636365No Hit
GTCATGCTCTGCAACACGCCATCGCGTCGGATCAGCCCATGAAACAACGC11.1363636363636365No Hit
GGCTGTTTTCACTGCAATAGCAGAGTTGAGTAGTTGTGACAAAGAGTTTA11.1363636363636365No Hit
TTGTAACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCA11.1363636363636365No Hit
ATTGTATAGTGGAGATATGGTTAGGCCGTGTGTCCTCAACCAAATCTAAT11.1363636363636365No Hit
AGTCTGGCTAGGTTAAAGGCCAGAGGCTATGGAACTTAACACTCCCCATT11.1363636363636365No Hit
TCCAACCTCAGGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTAGGATTA11.1363636363636365No Hit
GTTAGACAGAGGTATGGTGTGTCCAGGAGCCTGCCAGTGCAGCCTGCAAG11.1363636363636365No Hit
CTTGACAAGACATCTAAAAATGTTGGAACTGGACTGGTGGGGGCTCCAGC11.1363636363636365No Hit
TTGGCCAGGAATCGGGACTTGTCCCGGTCCCGGCGCTCGGCATCCGTGCG11.1363636363636365No Hit
GGTCCGGGAAGGCGCCGTCCGCTGCGCTGGGGGCTCGGTCTATGACGAGC11.1363636363636365No Hit
ACAATGTGGATTGTGCCCTCACCCAGCAATGTTTATGCCCTATCGCCATG11.1363636363636365No Hit
ATTTATGGGGAACCAAAAAAACACTCCCCAAATAGCTGTGAAAAAAGAAC11.1363636363636365No Hit
CACATGCTTAGTTTGGAATCATTGCAGTGTACCCAGAACCCTAGAGTGAA11.1363636363636365No Hit
GTCCGCCGCGGCGGCGCTCAGCTCGGCGGCGGTGCGGGCCTGCTCCGGGG11.1363636363636365No Hit
GCAGTAAATCTTTTCCTTTAGATCAGCTTGTTTGAAATACGATGGCACTT11.1363636363636365No Hit
AGGGGAGGCTGGTAGCACTTTCCATGTTCTTCCCAGATGTCTCCTTAGGC11.1363636363636365No Hit
TGTCCATCCCGCGATCATGCCGCAGAGCGAAAGCTCGGTTTCAGCGGGCG11.1363636363636365No Hit
GTATTATAGCAGGATACCTCCTGAAAATTGAATTTATTCCATTCTTTTGC11.1363636363636365No Hit
GTTGGCAGTTGGGTGGTTTGAGAAGATGAATTTCAAGTCCCTATCAGCCT11.1363636363636365No Hit
CCGTTATCTGATGACGGTCATCGCGACCGCCTCCAATCCCCGGTTCACCG11.1363636363636365No Hit
GGCCAGGCTGGTCTCAAACTCCTGACTTCAGGTCATCCACCCGCCTCGGC11.1363636363636365No Hit
GTCCAGGATATACAGTATCTGCTTCATGTCCTGGGGTCGTGATGAAGGGT11.1363636363636365No Hit
ACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAAAAAAAAAAAAA11.1363636363636365No Hit
GAGTACATGGGGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365No Hit
CCTGTAGTCTCATATTTTTTGCAGCACTACTCACAATAGCAAAGATATGG11.1363636363636365No Hit
TGAGTTAGGTGCTATTATTATTCTCATTTCACAGATGAGGAAACAGGCTC11.1363636363636365No Hit
CATCGAATGGAATCGAATAGAATTATGGAATGAAATCCAGTGTGATCATC11.1363636363636365No Hit
CTGATTGATTCCATTCCTTTCCATTCCACTCGAATACATTCCCTCCTATT11.1363636363636365No Hit
GTCATGCTTTGCAACACGCCATCGCGTCGGATCAGCCCATGAAACAACGC11.1363636363636365No Hit
ATATGAAGAACATCAAATCCTACATTGCAATCGCCGATCCGTTGAGGGGG11.1363636363636365No Hit
GAGTGGAATGCAATGAAATCGAATGGAGTGGAATCGAATGGAATTGCACG11.1363636363636365No Hit
CAACATGCAATCACTAGACTAACTCTTAACATACACTAATAAAAAACTAA11.1363636363636365No Hit
GTTCCTGCCAGGAAATCTTCGACAAGGATGCGCACATGGACCGGATGACC11.1363636363636365No Hit
GCCCATCAACGTCCCGGCCCGGCCATTGGCCTGGGCCACACGAACAGGAG11.1363636363636365No Hit
GCACGGGACACGTGCAGAGGCCACGCGTGAACGTTGCTGTACCGGCCGCG11.1363636363636365No Hit
CTCCAAAGAACTCTGAAATTTGACAGAATTTCCTAAAAATGCAATAAAGG11.1363636363636365No Hit
GGCCTTCGGGGAGCGCCCGGTACGCGGCGCCCCCCAGCGGCTGTCTCTTA11.1363636363636365No Hit
GTGTAAACAGGCTGCCAAAACCCACCCATGCTGCTACAGAATCGAGGGTG11.1363636363636365No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA11.1363636363636365No Hit
CTATAGATAATCAACTTAAGAAGAACTGACATCTTGACAATATTGAGTCT11.1363636363636365No Hit
CAGTAGTACTTACCTTATAAAATCACCTGTAATGTCAGCTTGAAAATGTC11.1363636363636365No Hit
GCCCTAGTTGATGCTCACTCTGTAATGATGGGCTGATTACTTTCTAAGCT11.1363636363636365No Hit
ATTTCAAACTCCTGGCATGTTTAGACGCTATTTGCAGAGAAGGTAGAGTG11.1363636363636365No Hit
GGTCAACACCCTGACTAAGGCCAAAGTCAAGCTGGAGCAGCAAGTGGATG11.1363636363636365No Hit
CAACAAATCCCAGGGAAGAAAGCAGCTGACAATAGACTTCTCACATCAGA11.1363636363636365No Hit
CTCTGCAGATGCCCCAGCTGCTCTCTACCAGACAATTGAAGAAAATATTA11.1363636363636365No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)