FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGTCTGACTCCACAAGCACCCACACGAATTGCTTGATTCAGTTGTTA44.545454545454546No Hit
TAACAATATTGTGCAAGTATATTTAAAATATATGAAAGTTTCAAATGAGT33.4090909090909087No Hit
TGTGGAGGCTGCGTGAGGTTGTTGCCTCAGAGGATGGCTGTCTTGGGTCT33.4090909090909087No Hit
GGTGTGAGCCACCACGCCCGGCCTGCCTCCAAGAAACCATACTCCTCCAA22.272727272727273No Hit
CTGCTGTTTATACAACCATGCTTCTTCCAAGTCTATTTTTGGTTATGTTT22.272727272727273No Hit
ACCGCCTCTGCCCCAGGAGCTGCCGCAGTGGCAAGTGCTGGCGGCAAAGG22.272727272727273No Hit
GGTGTGTTTTTTCTGCCCCAGTTGTATCACAAATTCCTGTGGGGCAAGAC22.272727272727273No Hit
ACTCTGCACAATAACTGGTCTATAATCTTCAAAAGTGGCAATGTCATGAA22.272727272727273No Hit
GGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCT22.272727272727273No Hit
CAGAAGGGTTAAGGCCACAAAGGAAAAGGAGAAGGGATGGGGTCTGGGGA22.272727272727273No Hit
GTGAAGATCAGCGCGCGGTCGATGTCCGACGTCTTGATGATGTGGTGAAG22.272727272727273No Hit
CGCCTGAACATGGTGGCCGAGGACAACGGCACCGCCGAGCTCATGGCCGA22.272727272727273No Hit
GGCTATATTGAACTAAGTCTGAGAATTCTAGAATTCTAGGTAAAGCAGAT11.1363636363636365No Hit
AGATTGTCTTTTCTTCAATGTATGTTCTAAGCAACTTAAAAATTACTTGG11.1363636363636365No Hit
GATTCAGTCAGGGCCCTCGACCTCGACCGAATTCGCGCTGTCGTGGGCAC11.1363636363636365No Hit
GGATTCAGAACAATGAAACTGTGTATTTTGCTTGCATGAGAGTCATAACG11.1363636363636365No Hit
CGTCACGGTGAAGCCACATTCTTCCATGATGGACAGCTTGGCTTCGGCCG11.1363636363636365No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA11.1363636363636365Illumina Single End PCR Primer 1 (100% over 21bp)
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATTAAC11.1363636363636365Illumina Single End PCR Primer 1 (95% over 23bp)
ATTCCATTCGAATCCATTCGATGATGAGTCCATCCATTTCAATTTCATGA11.1363636363636365No Hit
GTGGCGAAGGACGGCGTGGGTGGGGTGATTACAGCCGGCGCCTCAGGGGT11.1363636363636365No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA11.1363636363636365No Hit
GATAAAGGGTGGTTGTGGTATCTGGAGCTGGAGTTGGGAAGACAGAAGGA11.1363636363636365No Hit
CATCGTGGCCGCAATCGCAGCGAATGGGGGGCGGGAAATCCTGCGCGAAG11.1363636363636365No Hit
GAAAGGTCCATGATCCAGGTCAGATGATTTGCTTCTAGTTGTCGCTGTCA11.1363636363636365No Hit
ACTGTGAGGCTGACCTGTAACTGGGCCTCAAGGAGACCATTCATGCAACC11.1363636363636365No Hit
TTCCATTCCATTGCATTCCATTCCATTCCATTCCCCTACACTCGCGTTGA11.1363636363636365No Hit
GTACCAAGCAGAGTACCTTTGAAATTTTTTTTCATTTAAAAAATACGGAT11.1363636363636365No Hit
CTCGACGCCTTCCACATCGCCGGCCTTTCGCCGGTGGTGACGGTGAGCGG11.1363636363636365No Hit
GCCTTGATGTTAATAAGTAACTCCAGTAGGTTTCTGGGTGGCATAACAAT11.1363636363636365No Hit
CACTTCTGTTAGAAAAGTTAAAAATTAAAGTGCACTTAGTAACAGCCTTG11.1363636363636365No Hit
TTATATTCCTTGGTTTTAGCTCCTAAACTCTAAATTCTAATTTTCAAAAC11.1363636363636365No Hit
CTATAGTAATGTCTCCTGTGGCCTTTATTAAAGCCTTCAATATAATAATT11.1363636363636365No Hit
GAATGTAATGGAATGGAATGCAACGGAATGGAATGGAATGGAATGCAATG11.1363636363636365No Hit
GACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGTTCAC11.1363636363636365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTCTTTTAAAACCC11.1363636363636365No Hit
GTAAGGTCCATGATCCAGCTCAGATGATTAGCTTATAGGTGTCGCTGTAA11.1363636363636365No Hit
ATTAATATGTCTCAGTGGATGGGCAGTCCTATTACAGCTGGGGCAGATGA11.1363636363636365No Hit
GTGTATTACGTAAATATGTAAAGATTCTTCAAGGTAACAAGGGTTTGGGT11.1363636363636365No Hit
GAGACACCATTGTAAGGATGCTATCTGTTAATCCTTTAGAAAGAGGTAGT11.1363636363636365No Hit
CTCCATCAGGGTCACCGCATCGGCAGGCAGCTGCTGCACCGCATCGGAGC11.1363636363636365No Hit
GGGAGGCATCAGGAAACTTACAATCATGGCAGAAGGCAAAGGGGAAACAA11.1363636363636365No Hit
GTACGTCATGGTAGAACACCATTCTCGCTACGATAGCAAAACAATAAAAG11.1363636363636365No Hit
GGCTGGTCTCGAACTCCTGACCTCATGATCCGCCCGCCTCAGCCTCCCAA11.1363636363636365No Hit
TTTTTATTTTACATGTGAGAAATCAAGCATGGATAATTTATAATCTGCAT11.1363636363636365No Hit
TAACAGACCTGCATAGGTACCCCCCGAATAATACTCTGGGAAAAAAAAGA11.1363636363636365No Hit
CGAGATGGCCCGGCGCAAGCGACGGCGTCGGGCCCTCGTGGCCCTCGGGG11.1363636363636365No Hit
CCGCTGGGGGGCGCCGCGTACCGGGCGCTCCCCGAAGGCCCTGGCTCTTA11.1363636363636365No Hit
TCGCTGGTCTGGCCGATGGCGGCCTCGGCCTCGTCGAGCAGGTCGAGCGC11.1363636363636365No Hit
CTCTATTATTGATCATACCAGTGAGATTAGATACATAATTCAGAATTGAT11.1363636363636365No Hit
GTTCCATTCCATTCCATTCCATTCCTTTCCATTCCATTCCTTTCCACTAG11.1363636363636365No Hit
GGATTCTTCCTGAGCTACAGCGGCATCGCATTCCAATACGTCTGGCCTCG11.1363636363636365No Hit
AAAATACATAGTCATATTTGGGCTTTGTTAACTAGTTACAGAATCAAGAA11.1363636363636365No Hit
GTCCATTCCATTGCATTCCATTCCATTCCATTCCCATACACTCGAGTATA11.1363636363636365No Hit
GGAATAGTCTTGATAAGTAGCATATCCTGTTTGCCATTATCTATTTGTGT11.1363636363636365No Hit
GGGTAACACTGCCCAATCTATATTTCTCATTAAAGAAAAATGAATTGGCT11.1363636363636365No Hit
GCTGGTGGCCGTAGCAGATGCCCAGCACCGGCGTGTCGCGTGCGACCAGT11.1363636363636365No Hit
GGGTTTCACCATCTTGGCCAGGATGATCTTGAACTCCTGCCCTCGTGATC11.1363636363636365No Hit
GTGGCGAAGGACGGCTTGGGTGGGGTGATTACAGCCGGCGCCTCAGGGGT11.1363636363636365No Hit
GAGTTATAGCTAATAAGCCAATGAAGGAGATCAACTATCTTTGTTCATAG11.1363636363636365No Hit
GGTACCAACAGGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAATACTCAG11.1363636363636365No Hit
AGCTAATTGTAGAAATTAGCCGAATGTGAGAGCAAAGTGCATGTGGGAAG11.1363636363636365No Hit
GTGCAGAATATGTAGGTCTGGTACATAGGTAAACATGTGCCATGGTGGTT11.1363636363636365No Hit
GTTTTTTTGTTTGTTTGTTTTTTGCCTTTGATGACTTTAGGACGTGTGGT11.1363636363636365No Hit
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATTTAC11.1363636363636365Illumina Single End PCR Primer 1 (96% over 27bp)
TGCTTCTACCAACCAGGTGAAGTAGATATTTCAGAAAAAGGAGGCTTAGA11.1363636363636365No Hit
TAGTAATGCTGGATAAGTGAATGAATTGGTGAATGAGTGAACAAGTGAAT11.1363636363636365No Hit
GTCCAGCGATGCGGTGGGCTGTGCGTCCGACCAGGCGCGGAACAGCAGCA11.1363636363636365No Hit
CCTCCTCGATCACCGCGTTGATGGTGTCGGCGTCGGTCTCGGCATGGGCC11.1363636363636365No Hit
GAGTTACCATCTCTGTCCATAGAAAGCATTCAGTTTTATTCTGTATAGAT11.1363636363636365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA11.1363636363636365No Hit
ATTCACCCACGTTTCAGAGGTGTCTGACGATGCGGGGACGCCTGCCCTGG11.1363636363636365No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)