FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTAGGCGCCCGGCAGCAGCACGGCCACGGTGCCCGAGTGGCGCAT32.7027027027027026No Hit
TGCAATTTTCAAGTAGACAACGTATCCTAACACCTTTCTTTACAAAGCAT32.7027027027027026No Hit
TGTTGATGCCGAACTTCAGCGAGGCGTCGATGCCGAAGTCCTTCAGCTTC32.7027027027027026No Hit
CGTCGGCGCGTCACGCCACGTTCGTTCGGCGCTCGCGCCGGACGGTGCGT32.7027027027027026No Hit
GGGCCGCCCCATCGAGGCCCGTCTCTGCCACCAGCTTCTCGACCAGGTCG32.7027027027027026No Hit
TCAAGGGGGACAGCAGGGCGGCCATCAGGGCATGTTCCATTCGGGAGCGC21.8018018018018018No Hit
GAATTGCAGCAAGGAATGCATATTCTAGGCTCCCCTCCACCTCCTAGGCT21.8018018018018018No Hit
GAATATTACTTGTGTAAGAGTTGGGTACAATAGGTACCATGGGATCTTAT21.8018018018018018No Hit
GTGCTCATGTTGTTGCCTTCGCCGATGCCCTTGGCGCCCAGCGGCGTGTA21.8018018018018018No Hit
AGAAGAGACAGACAAATAGAGAAAGAAAGAAGGAAGGAAGGAAGAAAGAA21.8018018018018018No Hit
GACCAGATCACCACCGATCAGGCGGTGGTGCCGCTCTATCCGGTCGAAGT21.8018018018018018No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACTACACGACTCTCTTAA21.8018018018018018No Hit
GTGTGTATGTTGAAGGAAGAGCTTAATCAGGAGGCTAGTGTGACTGGTGT21.8018018018018018No Hit
GTATTATCCTAAAAAAATTATTTGTTTATCTGAAATTCAAATTTAACTGG21.8018018018018018No Hit
GACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGC21.8018018018018018No Hit
GTCCTCGGCTGTCGGCATCGGCGGCGACCCGATCAATGGCCTGAAGCACA21.8018018018018018No Hit
TCTCCTCTATGACTGACAGCAGGTCCGTGTAGGTGCTCTGGGAGCCCTGG21.8018018018018018No Hit
GTACCAGCTCCTCCTTGTACCTTTGGTAGAATTCGGCTGTGAATCCATCT21.8018018018018018No Hit
CACGATCTCTGCTCACCACAACCTCTGCCTTCCAGGTTCAAGCGATTCTC21.8018018018018018No Hit
GAATCGAATGGCATCGAATGGAATGGAATGGAATGGAATGGAATGGAATG21.8018018018018018No Hit
ACGCGATTCTTCGGCGACCCGTTCTTCGCGTCGATCGTCTCGGGCATCAA10.9009009009009009No Hit
GGGGTGGAGTCTAGGGGACTGCGTTGTGTCCCAGCCCTGCTCTCAGGGCA10.9009009009009009No Hit
TGCCAGCATCATGAGAAAGCGTGCCGGCAAAGAACGCCAGCGCCCACCGG10.9009009009009009No Hit
GTTGAGATAAAAAAATTCGTTGTCATTTATTCCCAAGCACAGCTGTTTCT10.9009009009009009No Hit
ATTCAATTCCATTCGACTCTATTCCGTTCCATTCAATTCCATTCCATTCG10.9009009009009009No Hit
CTTTACGCGGCGTCAAGTCATCCAGGTCAAGCTTTGCCAACTCATCCAGC10.9009009009009009No Hit
GGACACGCGGGTGCGCAAGGACATCAGCGAGGGCCACGCGGGGCTGCCCG10.9009009009009009No Hit
CTGCCGCATCGTGGGCAGCAGGGGTATCGCCTGCATCGACTGAGTTCATC10.9009009009009009No Hit
ATTCTTGACTGATAATTATTTTCTTTGAGGAGGCTGAAGATAGGGCCCCA10.9009009009009009No Hit
TTGTTATGCCCCTCACTTGCTTAATTTAAGAGCCCAGACTTGTGTGGACA10.9009009009009009No Hit
AGCTTGGTCTGAATCCCGCAGCCCTGGTGCGCAGCGCCGCAACCGGTCGC10.9009009009009009No Hit
CTATAGTGGAAAGCGTAAGAAGCATAAGATTAGCATAGGCCAGCAGTAAG10.9009009009009009No Hit
GAGGAATAGAAAGGGATAGGGCTGAAGAACACAGGTCGCTGCATTTAGAA10.9009009009009009No Hit
ATTCCGGATGATTCCATTCCATTGCATTCCGTTCCATTACATTCCCCTGC10.9009009009009009No Hit
TCCCTGTCTTGTGCCCGTTTTCAAAGGGAATGCTTCCAGTTTTTGCCCAT10.9009009009009009No Hit
CGCCCGCACTTGGTGGCTCAAGCTGGCGGATTGAAGACATCCGAACCTCC10.9009009009009009No Hit
GAGTAGAGGAAGCCTCCACCCCAAAGGTCAACAAGCAGGGTCGGAGTGAG10.9009009009009009No Hit
GGGAAAACTGGTTATCCTCATGCAGAAGAATAAAATTAGACCCTCATCTT10.9009009009009009No Hit
GTTTCGCAATGAGACCATGGAAATTCGAGTCGGCATACATTTCCAACGTA10.9009009009009009No Hit
GAATCACTCCCGCCCTAGACGACGCGGCAATCAACGCACACGTCTATTGG10.9009009009009009No Hit
TTATTGAGCAGTTTGGAAAAACTTTTGTTGTAGAATCTTCAGGTGGACAT10.9009009009009009No Hit
CCCTAACATCCCAATTAAAAGAACTAGAAAAGCAAGAGCAAACACATTCA10.9009009009009009No Hit
GTATAAGGGTATGTTGGTCAAAGGAAACAAAGTTTCAGCTGTAGAGTTTG10.9009009009009009No Hit
CGTCGGCCCGTCACGCCACGTTCGTTCGGCGCTCGCGCCGGACGGTGCGT10.9009009009009009No Hit
GGATTTATTTAAAATAATAATGGCCAAGAACTTTGCCAAATTAGTGGCAG10.9009009009009009No Hit
CTCTAGTGGAAAGCGTAAGAAGCATAAGATTAGCATAGGCCAGCAGTAAG10.9009009009009009No Hit
GCTCCAGCTGCCGCGCCCCGCGCAGGTCGTCGCGTTGGCCAGCCTGCTGC10.9009009009009009No Hit
AAGCTGGAGAAAGTGATCCAGATCGAGTAGCTGTCAGCTCCAGGCTGGGA10.9009009009009009No Hit
TGCAATTTTCAAGTAGACAACGTATCCTAACACCTTTCTTTACAAAGCAA10.9009009009009009No Hit
CCTCTATGCAAATAAACTAGAAAATCTAGAAGAAATGGATACATTCCTCG10.9009009009009009No Hit
CATCTAAGGATAGATTAAGGAGAGCTGTGAAGAAAGGGGGGAGTGTGGGT10.9009009009009009No Hit
GGCTGTCGATGCGCCATTCGTGATTGGCGATGGCCGCGCCGCCCGAGGAG10.9009009009009009No Hit
TATATACCCAGAACTGGGATGCTAATCATATGATAATTCTATTTTTAGTT10.9009009009009009No Hit
CCTTATATAGTCTCATTATTAATCACTTGTCAGATGGAGAGTTTGCAAAT10.9009009009009009No Hit
GACAAGGGCCTGGCGCTGCGCTCCGCATTCACGGTCGTGCTTTCCACCTG10.9009009009009009No Hit
GGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAA10.9009009009009009No Hit
CGGAAGAGCGTCGTGAGGGAAAGATGTAGATCTCGTGCGGACCCATGTAC10.9009009009009009No Hit
TCCCTACACGACGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9009009009009009Illumina Single End PCR Primer 1 (95% over 22bp)
AGACAGAAGCATTCTCAGAAACTTATTTGCGATGTGTGTTCTCAACTAAA10.9009009009009009No Hit
GTCCCGAGGGCAACCTGGGCGGCGTCGACATGCGCCGCTCGATCCAGCTG10.9009009009009009No Hit
CGACAGCTGCTCGCGCAGGCTGGGTGCCAAGCTCTCGGGTAACATCAAGG10.9009009009009009No Hit
GTAATCAAGTTGCCTTCGCCATCCGTACTACTTGTGCCGACTGGATCTGT10.9009009009009009No Hit
GTCCTCATCGAACAATCAACTTTCACGCTACGCCATCGTGCTGGGCCTGC10.9009009009009009No Hit
GAATCAGATTGTCTGATTTCCATTCCTGATGTTTTTACTTACTAGATTTG10.9009009009009009No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAA10.9009009009009009No Hit
GAAGATGAGGTTGCGCGCCGGGACATAGGTGATTGGGACGTCATCGCCGA10.9009009009009009No Hit
AATTCGATGCATTAGCTGCCGTTCCCGCCGACACCCGCAAACGATAGACG10.9009009009009009No Hit
TCTGGAGGGTCGGGCTTGACTGACGGACAAGCAGGAAGCAGGGTCAGGGG10.9009009009009009No Hit
GCAGCTCGGTTCTGCTGGATGGGAAAAGCTGAATCTCAAAGCCTCCGCTC10.9009009009009009No Hit
CATAATGTGTTTCCAAGGTCTTTTTACTGAGTTTGTGTTAGTAGCTCTCT10.9009009009009009No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCCAAACACGAAGAAAATAA10.9009009009009009No Hit
CCCATACCCGCTTGCGCAAGAAGGACTGAAGCCCATGCCTGAACTTCAAC10.9009009009009009No Hit
AGTCTTCAAGTGAATATTTGGCTAGATTTCAGGATTTCATTGGAAACGGT10.9009009009009009No Hit
CTAGCGGTCTATCAATTTTGTGGATCTTTTCAAAAAACCAGCTCCTGGAT10.9009009009009009No Hit
GCTCTACAGCGAGGAGTTCGACCGCCTCGCGCGCGAGCGCATCGGACTCA10.9009009009009009No Hit
GAAGAGGCCAAGTGATGGAAATAGTGGATTAATGGCAAAAAAGCAAAAGC10.9009009009009009No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAA10.9009009009009009No Hit
CCATTCCATTCCGTTCCGTTCACATCAATTCCTTGCGATTCCATTACATT10.9009009009009009No Hit
GTGCATGAACTGCACGCCGGCAGGGCGGTGCGTTTGCTGGCGATGGCCGA10.9009009009009009No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT10.9009009009009009No Hit
GCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTCCATCTCAAACAAA10.9009009009009009No Hit
GGGCTGGGATGATGTGGTGTGCAGACAAGTCTTATCACGCGTCTGAGCCG10.9009009009009009No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA10.9009009009009009No Hit
GTACTCCTTGTACTGCTTGCCCTGCGTGGTGGCCTCGCCCGCGCGGATGC10.9009009009009009No Hit
AGTCGATACTTTGACCAGACGGACTATGAACTCGAGCTTGAAGTCACCGA10.9009009009009009No Hit
GCCAGACACAGATCAGCAAGGAGGAGAGAGGGCTTCCTGGGCAGGAGAAT10.9009009009009009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)