FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGCATGACCGAGGACCTGTTCCCCAAGGAAATGCTCGAGGCCTTCCG52.9940119760479043No Hit
GGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAA42.3952095808383236No Hit
ACATTAAGTGCATGACATACTACTCATAAAAAAAGTGTTGGCCGTGCGTG31.7964071856287425No Hit
GAGCCATGTTGACCGAGGGACTTGGCAACGCGTGAGACACCCGCGGCCCC31.7964071856287425No Hit
CCTCTTTCTTGGTGTCGGAAATGGGGACTCGCGGTCTGGGTCATATCTCC21.1976047904191618No Hit
GTGTATGAATTCGATAGAGAAACCGTACACAATAGAGTTTCTTAAGCTGG21.1976047904191618No Hit
CTGAGCATGGGGATCATGCATCGGGCCAAGCTGGCCGGCTTGCCCGACGC21.1976047904191618No Hit
GACATGCACAAAGCGGTGAACCCTGCTGTGGCGCCGGCCGCTAGCTTCAA21.1976047904191618No Hit
ATTCGACCGCCTGCCCGAGGGGTCGATGCTCTCCATCACCGTCGTGGTGG21.1976047904191618No Hit
GGTGGCAAACTCCCTTCCACCTACCTGTGCAGAAGGTGAGTAATGCATGA21.1976047904191618No Hit
GTATTACTGTGTTCTCATGCTGCTAATAAAGATACACCCGAGAGTGGGTA21.1976047904191618No Hit
CTTTTATATGTTTTAGAAGTGGTCATGGCCAGGTACGGTGGCTCACACCT21.1976047904191618No Hit
GGGTTCAAGCGATTCACCTGCCTCAGCCTCCCAAGTAGCTAGGATTACAG21.1976047904191618No Hit
GTAATCTATCATCCCTCCTTTACTCCATTTCAGGCAACGACTGGCTTTGC21.1976047904191618No Hit
TTCCATTCGAGTCCGTTCCATTCCAGTCCATTCCATTCGAGTCAATTCCA21.1976047904191618No Hit
TTCCAGGGTGGGGCCGAGGGAACCCAAGGTAGAGGGAGTCAGCGGTCCGC21.1976047904191618No Hit
GTCCCCAAAGTCCATTGTGTCATCCTTATGCTTTTGCCTCCTCATAGGTT21.1976047904191618No Hit
GCTACTTCAACACTGGAACAGATCACAAAAGAAGGTGATGAGTGTTTAAA21.1976047904191618No Hit
CTGCGGAAACGGAAACTCCGAAGTGGTTTGCGGCTAAGATGGGCCCGCCT21.1976047904191618No Hit
AGCCACCAGCTTCATGCTTGCAAAGGAAAACCACTTCCCCCTTTTTCTTT21.1976047904191618No Hit
GATCTCATGGTTGCTCTTGCCCTCCAGCAGCCAGTTGAGCACCTGCAGCT21.1976047904191618No Hit
GTCATCAGAAAACCGGCGGTGCCGGACGGCGAAAGGGCAATCAGGAGTGA21.1976047904191618No Hit
GCTCTCCCTCATCATCATCGCCGTCCCCACCTTCGTCCTCGGCTTCGTAG21.1976047904191618No Hit
GTTATTATCCCCATTGCACAGATGAGCAATTGAGGGTCCTGGAGATGACG21.1976047904191618No Hit
TGCCTACGGCGCAAGCCACTACTACCACAAACATAACCAGTACTGCTATC21.1976047904191618No Hit
TAATAATAATAATAATGATGATGGCAAAAAATAAAATTTGAAATGTGCAA21.1976047904191618No Hit
ATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCGCA21.1976047904191618No Hit
GTTTGTGGGTGCCCTTTTTCCCATCTCATAACTTGTTTTAAGAAGCGCAG10.5988023952095809No Hit
TGTAACGGCATGCCGGTTAAAAAACCGGCTAGTGTAGTCTCTTCTGATGC10.5988023952095809No Hit
GTTTCGTGGAGGCCATTCACGTATGTGCCATTGAGAGTGTAGTCACGCCC10.5988023952095809No Hit
GGTGTTTTCCCCATCCTTTTGCCATGATGTGTCTGGAAATAGAACCGGAT10.5988023952095809No Hit
TTCGCCGTCAACGCTCTGGCCGACCATGACCAGAAGAACGTCATCACCGC10.5988023952095809No Hit
ACATACACAGAGCGGTCACTAGGTGTACCAGGCATTGCACTAGTTAGCTA10.5988023952095809No Hit
CCTACGTGGGACAAACCGACGTCACACTTATGTACTCCGTCGTTGACGTG10.5988023952095809No Hit
GGATCCATTAGAGAAGGAAACATTGAAGATGCAGGAGGGAGAAGCAATAA10.5988023952095809No Hit
AATGTGGGTCAACAAAATGGTAAAATTAAAATACACACAATAGACCACAT10.5988023952095809No Hit
CTCTCGTACTGAGCAGGATTACCATTGCAACAACACATCAACAGTAGGGT10.5988023952095809No Hit
ACAGTGGTAAAAGCAGTAGCCGAAAAGGTGGCAGCACTGAGACCTGTTTG10.5988023952095809No Hit
GTATAGGAATAACGTGCACGTCACGCCGTCGCGGAATGGCCATAGCGCCA10.5988023952095809No Hit
GTCTAACGTGGCGACGGCTTTCCATGTCATTGCGCATCATGTCACCACCC10.5988023952095809No Hit
AGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGG10.5988023952095809No Hit
GTTTATAATATACACAAAGGGCCTGAGGGGGCAGAGTCACCTTTGATGGG10.5988023952095809No Hit
GTCCAGCTCATCTTTGAGCTCAATAAGCTGCTCGCGGGAGGCTTTAAGAA10.5988023952095809No Hit
CCTTATACTACTAATCCAATATAAGGTGAACTCAGCATCTCGTTTCTGCT10.5988023952095809No Hit
TTCTAATCGTTGTTTTAACACCACTTCTTCAAAAGTTGTTCTTTCAGAAT10.5988023952095809No Hit
ATGTAATTGATTCGATTTCGAATGGAATCGCATGGAATGTAATCGAATGG10.5988023952095809No Hit
GAAATGAAGGTCAACGGGTGTCCCTCATGCACCGCCGGTTTGACGATCTG10.5988023952095809No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCGGGCCG10.5988023952095809No Hit
CTCCTTGCTCCAAGGACTGGGGTGGCCATCCTTTAGAGGCCATTGTGTGG10.5988023952095809No Hit
CCCGCCGCAGCGACTGAAGATATCGGCCGTTTGCGGTTAACCCCGAGATG10.5988023952095809No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG10.5988023952095809No Hit
GTTGACGACTGGCCGAGCCACCTCCTGGAGTACCTCGGACGCAGGTGTCG10.5988023952095809No Hit
CGTATAACCCTCTTGCAAATAGCAGAGTGAGTATAGCAACTTCCACAAGT10.5988023952095809No Hit
GGTATTAATCATCAGGTTTTACCACGGGCCCGCAGGTGTGGGGCATGTTG10.5988023952095809No Hit
CTCTTCTCCAAAGCAACGTCGCAGAGGGTAGTGCGGTAGAGAAAGCTACA10.5988023952095809No Hit
CCATGCGTCTGGCGGCGTCGGACAAGAAAAAACCGGCAAAAGCGAAACGC10.5988023952095809No Hit
TAATGCTTCTATTCTCGCACGTTTGGGCAGGCAGAGCTATTCCCAGATAG10.5988023952095809No Hit
GTTCCATTCCATTCTATTCCGTACCATTCCTTTCCATTCCATTCCATTCC10.5988023952095809No Hit
CCTGAGCCGCACGGTCAGAACTCAGATACTGACCGGCAAGGAGCTCCGAG10.5988023952095809No Hit
ATCTTACCAAAAAGATACGAAGCTGATTTCTACTATTGGGGCTTAATTTT10.5988023952095809No Hit
TTCCTCCTTCTCCTGACTTAACCTTGGGTGGCCGCAGGTCTTAAGGCTCT10.5988023952095809No Hit
GGTGATGCTGGGGCTGGCCGTGGAGCGCCTCGGTTCCCGTTCGTTGACCT10.5988023952095809No Hit
GAATGGAACGGACTCGAATGGAATGGACTGGAATGGAATGGATTGAACGG10.5988023952095809No Hit
CATGAGAGGTGTAGAATAAGTGGGGGGCCTCCCCCTTATTATACACCTCT10.5988023952095809No Hit
GCGTAAGAGATGATGAAGCGGTCACTGGGCTCGGTGACCTTCGCCGCTGT10.5988023952095809No Hit
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCA10.5988023952095809No Hit
CCTTGCAAGCGATATACCTCTTGTACTTCTTGGACAATATAGCGCGTTAG10.5988023952095809No Hit
CCCAAGGACTCCAGGGCACAGGCCCTCACACTCCCAGCTGTCTTCTCGCT10.5988023952095809No Hit
CGTTTCTCTGTTCTGCACTGAAAAATTTACTGAAATATTCGTCTGTACTC10.5988023952095809No Hit
TTGAAGCACTCTTTTTGTAGCATGTGCAAGTGGACATTTGGAGCGCCCTG10.5988023952095809No Hit
ACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC10.5988023952095809No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCCGCCGG10.5988023952095809No Hit
CATTAAAAAAGGTACAACAGTGAAATTCTCATTTGTATTTGTCACTCTGT10.5988023952095809No Hit
AAGACTTGCTCAACGCCGCCAAAGCGGATGTGCCGATGTGTTCTTCGGCG10.5988023952095809No Hit
GCTGCGCGCGGTGATGCATGCGGCCTCGGGCTGGTGGACGATCCGCTTCA10.5988023952095809No Hit
CCTAGCAACGTTATCACTAAAGGTAGCCGGTGACCGCTCCATACAGACCA10.5988023952095809No Hit
CCCTTACAAGAGTCCACTGTGGGCCAGGCGCGGTGGGTCACGCCTGTAAT10.5988023952095809No Hit
CTTTGTGATGTGTGCCTTCAACTCACAGAGTTTAACCTTTCTTTTCTTAG10.5988023952095809No Hit
ATCCAAAGCCCCAGCTTCGTATGTCGTGGGAAGCCCCACAGAGTGAAGTA10.5988023952095809No Hit
GCGCGGCGCGCACCGCGCCGGCCGTATCGCGCACCGGCACGGCCACGGCC10.5988023952095809No Hit
GTAGAGATGCGGCTTCACCATGTTGGCCTGGCTGGTCTCAAACTCCTAAC10.5988023952095809No Hit
AGGTTAGGGAAACTGTTCTTTGCCTGTTATTGTTTTTTATTTTAAGTGTT10.5988023952095809No Hit
GAATGAATATTAAGGAATAGCCATGACATCAAAACCTATGGAAAGAAAGG10.5988023952095809No Hit
TTGCATGGCAAAAGCTGAACAGTACAAATCCATTGTTTTGCTGGATGTGG10.5988023952095809No Hit
ACCTCCTCCTCGTCCGGCAGCTCGGCGGCGAGCCGGTGGGCCGAGGCGTG10.5988023952095809No Hit
GAATGGACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTC10.5988023952095809No Hit
GGATATGGGTTTGTTCACCAAGTCACGCGTGACACGCGCCATGCAGTGGC10.5988023952095809No Hit
GACGATTACATCGCGTTATCGAAAGAACTGGATGTTCCAGTGCGGATGAG10.5988023952095809No Hit
AGTCCATACGATGATTCCATTTGATTTCATTTCATGATGATTCCATTCGG10.5988023952095809No Hit
GATCTTTCTCTGTCACCCAGGCTGGAGTGCAGTGATGCGATCATGGCTCA10.5988023952095809No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA10.5988023952095809No Hit
GTGCAGCAATTCGTACGGTACAGAATGGGACCTGAAAAGACATGCAGAGG10.5988023952095809No Hit
GAATTGGCGAAGGAGGATTCCTCTTTCAAAGAAGAAGTGGCACGTCTAGA10.5988023952095809No Hit
GGCTGTCATAGCGCCACCACCGGCAAGCGTGAGTGGTGTGCGTAGGGTGA10.5988023952095809No Hit
ATCCCTGACTTTGGGATCTAAGTGAGAAGATTAAAGAAAACAGGAGACTG10.5988023952095809No Hit
CTGAAGGGCTGCGAACCCTGCGCCAGGCCGGGCTTGAAAAGGTCTGTCTC10.5988023952095809No Hit
ACCTGGCACTGTCCCCGGAGCGGGGGGGGCCCGCCCGGGGCCCCCCCCGC10.5988023952095809No Hit
TCCTTATAGGAAGAGAAGGTGCAGTGTCCGGGGTGAAGGGAGTTGTGACG10.5988023952095809No Hit
GTCTTTAACGGAACGATTTTGGAGAATCTTCTCCTTGGAGCCAAGGAGGG10.5988023952095809No Hit
GGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGC10.5988023952095809No Hit
GACATGATTTGATACGAGACCACGACTTGCAAGTATTTTTGGGTTTTGTT10.5988023952095809No Hit
CTGTCACCCAGTCTGGAGTGCAGTGGTGCAGTCTCGGCAGGAGAAACACT10.5988023952095809No Hit
GGCACTGACGTTCGGGTTCTGCGCCTTCTTCCTCAACCCCACAAGTCCAA10.5988023952095809No Hit
CAAGGTTGCCCGGATAGACGCGGCTGTTGAGCTTGCGCTCGTAGATGCGG10.5988023952095809No Hit
TCCGTTGACCGGCCAGTTGCTCGGCAACTACCCCCAGGGCTTCACTCATT10.5988023952095809No Hit
GGATTGGAATGGATTGGAATGGGAAGGAATGGAATTGAATGGAGTAACTT10.5988023952095809No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG10.5988023952095809No Hit
ACAGCCCGGAATCCAGTGATCGGCGTCGACCCCCGCATCCGGAAGTCCAG10.5988023952095809No Hit
GAGCATATGAGAATCGGGGGGAGTGAAGAGATGGCTTGGCTGCAGATATG10.5988023952095809No Hit
CCACTGTCGAGTTTGTATTGGGGTCAAGCGGCATTGCAGAAGAAAATGAG10.5988023952095809No Hit
TTTCTACTACACTCCATTCCAGTCCACACCATTCCACTCCACTCCACTCC10.5988023952095809No Hit
ACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG10.5988023952095809No Hit
CCTCATGAACTATCAGGAGGACAACAACAACTTCTTGCGATTGCATCGAT10.5988023952095809No Hit
GTGATGGGCAAGATCGATCACACCGCCAGCTTTGGCCGATTTCTTCGCCA10.5988023952095809No Hit
TTCATGCTCAAACAGCTAGAATGATGAGAAGGGCCTCACGTATGTGGAGT10.5988023952095809No Hit
GTCGTAGAACATGTCGGTGCGAATGGGGCCCGGTGCCACCACGTTCACCG10.5988023952095809No Hit
GCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACT10.5988023952095809No Hit
GCTTCTCTTTGGCATACGTGAATTGTGAGCTTCACTACTCTTGTGCTTCT10.5988023952095809No Hit
TCCGAGCACCGGCCCGAGCACCAGACCGACCCCGAAGGCGGCCCCGACGA10.5988023952095809No Hit
GATTGATTCCACATTCCACTTCCTAGATCTAGTTTAGAAAACATGTTCCC10.5988023952095809No Hit
GACATTGACCTCCTCACTGTCTTACTATAGTCCCATCCACAGCCACCTTA10.5988023952095809No Hit
GGAATCACCATCGATTGGAATCGAATGGAATCATTGAATGGACTCCAATG10.5988023952095809No Hit
GGCTAACTGCAAGCAACCTCCGCCTCCCGGGTTCAAGCGATTCTCTTGCC10.5988023952095809No Hit
ATTTCTGTCTTCTTTTATCTCCTCCTCATTCCTTCACTTTACTCTACTCT10.5988023952095809No Hit
GTTGGAGGAGCCTTCAGTTCACTTTCGTTGAGGAGGTCGAGGGAACATTA10.5988023952095809No Hit
TCTCCATATTGGTCAGGCTGGTCTCAAACTCCCGACCTCAGATGATCTGC10.5988023952095809No Hit
GCGTAGTCCACAGGGATGACCACATCGGGGGCGAAGTCACGCATAGCGGC10.5988023952095809No Hit
ACCTCCGCCTCGTCCGGCAGCTCGGCGGCGAGCCGGTGGGCCGAGGCGTG10.5988023952095809No Hit
TACAAGGTCTCCGGTGGTCTACACGGCGTCGGCTCCTCGGTTGTTAATGC10.5988023952095809No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10.5988023952095809No Hit
GCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAG10.5988023952095809No Hit
CAGAAATAACCAAATTTCCTTGTCAATTGTCTTTATCTATAACTATTTAA10.5988023952095809No Hit
GTGCAAGATTAGGCTTACTGGTATTGGGTTTTGGATACAAGGCATGTATT10.5988023952095809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)