FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACAAGAGCTTCGCCAAGATGGACCTTGCCGATTTCAGGCTGGTGGTCG52.9940119760479043No Hit
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT42.3952095808383236No Hit
AGACCGAGTTTCACTTGTTGTTGCCCAGGCTGGAGTGTAGTGGCACAATC31.7964071856287425No Hit
AATGTGGCTTGAAAAATCCAGCAAGAGCGGGGACCGCGGGTCCCGAGGCG31.7964071856287425No Hit
CTTTTAGGCGTTGCCGTTTTGCTGCTAACATTCAAACCCACAGGTGGCGA21.1976047904191618No Hit
GGTGAGGATGGGCGTGTGCGCGCTGCTGCAGGCCAACACCTCGGCGATGC21.1976047904191618No Hit
GTGCCATCCCGGCGACAAGGGTATCGGCGGCGTTGAGCACAGCCCAACCG21.1976047904191618No Hit
TTGTTGATCCGTCCGAACAGTTTCGGCATGGTGCCGTCCTCACCCGGCAC21.1976047904191618No Hit
CCTCCATACTGTAAACTGGACTATTACCACTCACACTTGGTTTGCTGCCT21.1976047904191618No Hit
GGTCATTAATTATCATCCACTATAGAAGGAAGATTGGTGAGGTAGTTGAG21.1976047904191618No Hit
GATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTC21.1976047904191618No Hit
GTCCTTTATAAAACCGTTAGATCTTGTGAGAACTCACTATCACAAGATCT21.1976047904191618No Hit
CTCCGACAGGGCCGGTACAGAAACATCCATACCGGGAAGAGAAACACCCT21.1976047904191618No Hit
TCGATGAACTTGAGGCCCGATGGCACGAGCTGGGCGTTGACGGCCGAGAT21.1976047904191618No Hit
GGTTACAGGCGTGAGCCACCAGCACCCGGCCTGAGTTAATACATCTGGTC21.1976047904191618No Hit
TGCTGGAGGGCAGTGGCACAATTTCGGCTCACTGCAACCTCCACTCCAGG21.1976047904191618No Hit
AGTCCTAGTTGGATGCCACCTTGGGATTTTATTCACCCACCACATCACAG21.1976047904191618No Hit
GTCAGTGAAGGTTCATCGATTGTAACAGATATACCATTCTGGTGCTGGAT21.1976047904191618No Hit
GAATGGAATGCACTCGAATGGAAAGGAGTCCAATGGAAGGGAATCGAATG21.1976047904191618No Hit
ACGGCGACGTGGTCGCGGTCGGGGAGCGGATGCGGGTGCGCGCGATCGCC21.1976047904191618No Hit
GTCGACGATTCACACTCGCCCATGAACTAGGGCACTTTTTCGAGCGAAAC21.1976047904191618No Hit
CTGTAAGAGCATCTGACAGGGCAGTCATCTCCATGATGCCCTAGCTGTGG21.1976047904191618No Hit
AATCAAAAGTTTATCTTCACAAGTCTGTAATCCCAGCACTTTGGGAGGCC21.1976047904191618No Hit
ATCTTCTGATGCGTATCGAGGTACAGACAAGCTCATTTTTGGAATCGTTC21.1976047904191618No Hit
GCAATATCCTCTGTCGTGCAGCATCTGAGTAACGTCAATTTGGCTCAATT21.1976047904191618No Hit
GTCGCCCAGGCTGGAGTGCAGTGGTGCTGTTTCAGCTCATTGCAACCTCC21.1976047904191618No Hit
CGGCTGAGGCCTTTGGCGGTGGCGGTGCGCACGTAGTCAGCCTCCATCAC21.1976047904191618No Hit
CTTAAACTTCACTTACCACCAAGTTCCTTGCCAATATCTGGACAAAACTC21.1976047904191618No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT10.5988023952095809No Hit
CGGCGAAGGTCACCGAGCCCAGTGACCGCTTCATCATCTCTTACGCCTGT10.5988023952095809No Hit
CAATACGAATTCATGACGAACAACTTTTGTAATTGCTCCCTCTCCTGATT10.5988023952095809No Hit
AGCCGGCGTTGTCAATCCGAACATCGTCAGGCCGACGACCGGTTTGTTGT10.5988023952095809No Hit
GAACAGCGCGCCGGCCGGCAGGGGGTCGTAGAAGAGGTGGCAGGTGCCGC10.5988023952095809No Hit
CCTTTATCATTTTTTATTGCATTTGTTTGATTCTTTTCTTCTTTACTAGT10.5988023952095809No Hit
CAATGACTCGACAATCAAATAACTATTTTTTTTGAAGCACATTCGGACTA10.5988023952095809No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG10.5988023952095809No Hit
CTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCA10.5988023952095809No Hit
CTTCAAAGCAGCAACACAGGTTCCCCATGAGAAAAACCAAACTCAGGGAA10.5988023952095809No Hit
GTACAACCTAAAATTAATCAAGCGAATTTAAAATCAATTGATTTTCCAAA10.5988023952095809No Hit
CTGTCAAAATATCCAAACTGCTGGTCGCCTCATCCAAAATCAAGACAGGC10.5988023952095809No Hit
AATTGTTTCATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGT10.5988023952095809No Hit
GAATTACTCAATCTCCCCAGAGGGAACCTACCTGCCCATCTGCCTCTGAG10.5988023952095809No Hit
GAATCAATTACATAGCAATCCATTACATTCGAGTCTATTCTATTCCGTTC10.5988023952095809No Hit
CCAGTGCCTGTGCATCTCAGGGTCCCCGGTACCCAGAATCCAGGGAGCGC10.5988023952095809No Hit
CACCGACGACGAATGGGCCGAAACAGCCGATGGCGGCGGTGAACCCGATG10.5988023952095809No Hit
TTTCTTCATTTATCAGTGAAAGAAGTTAGAAATTAACTTCCCAAAAAAAT10.5988023952095809No Hit
AATACAGACAGTGAAATCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT10.5988023952095809No Hit
ACTCGTGGCCGCGCAGTTTTTGAAAGCGCACCCAAACGTCGGTTTGGATG10.5988023952095809No Hit
TTTGAGACGGAGTGGCCCAAGCTGGAGTGCAGTGGTGCAATCTTGGCTCA10.5988023952095809No Hit
GTCAATGCCGGCTGGCTGCGATCAGTGGCGGCTGGGGTGCAACCTGACAT10.5988023952095809No Hit
CGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCGCCTGCC10.5988023952095809No Hit
GATGATGATCCCATTCGAGTCCATTCAGTGATTCCATTCGTTTCCATTCC10.5988023952095809No Hit
CCTCGACATCGATCAGTGTTTCATAAGCGTCGAGAGACTCTTGTGGAGTT10.5988023952095809No Hit
GAGAGTAATTCCAGGCTAGCGTGAGAGGCCCTGGTAGGTCAGAGTGTCTG10.5988023952095809No Hit
GACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCC10.5988023952095809No Hit
CCACAGAACAGAGTTGCTTTGAACACTTCACTGACTGAGAATTGTGGTAT10.5988023952095809No Hit
GTCTCAGAATTCAGTTGTATCGGAAAGGAAGAAATGGTCAACCATGTCCC10.5988023952095809No Hit
GAGGATCACCTGAGCCTGAGAGACGGAGGCTGTAGTGAGCCATGATCGCA10.5988023952095809No Hit
CGTCTCTACTAAAAATACAAAAATTAGCCAGGCGTGATGGGTGCGCCTGT10.5988023952095809No Hit
CTACCCCTTCTCCACTTTCCTGGGGGGCAAGCACCCCCCACCCCTTCTCT10.5988023952095809No Hit
TGATGGTGCTGTTCTTCATGAGCTTGTGCAGCGTTCAGTACAGGTAAAAG10.5988023952095809No Hit
AATCTCGCCGTTCCAGATTTGACGGAATGGTGCCGAAACTTGCGTCAGAG10.5988023952095809No Hit
CCCTATTAGTGGGTGAACCATCCAACGCTTGGTGAATTCTGCTTCACAAT10.5988023952095809No Hit
GATCAAGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTG10.5988023952095809No Hit
CCTGTATGCGGCGGAACTATTGGGACTTCCGGCCGCAGACTGTGCCGTGT10.5988023952095809No Hit
ACGTCACCTTGTTTCCACAGTTGGTCAAGGTAACTTCGGATGAGCTCTTG10.5988023952095809No Hit
CAGAGTAATTCCAGGCTAGCGTGAGAGGCCCTGGTAGGTCAGAGTGTCTG10.5988023952095809No Hit
GCTTGCACAGGGCTACGAGGTGGTGTCGCTCGCGCGCCGCAAGGCCGAGA10.5988023952095809No Hit
GACATTATCTTTTCCTACCTCAAATTGAGGCTTTACCATAAGCATCATTT10.5988023952095809No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT10.5988023952095809No Hit
GGTATATTCTAATTATACTACACACACAGTAATTCACATTATCCTTAAAC10.5988023952095809No Hit
GGTCCATATATCCACTTGCAGACTTTACAAACAGTGTGTTTCCAAACTCC10.5988023952095809No Hit
TCCCTATCTAATCCGTTCGATTCCACTCCATTCTATCCACTCCTCTTCAC10.5988023952095809No Hit
GCTATCCAAATATCCACTTGCAGATTCTACAAAAAGAGTGTATCAAAACT10.5988023952095809No Hit
TCCTTCCACCTCGGCCTCCCAAAGTGCTGGGATTGCCGACGTGAGCCGCT10.5988023952095809No Hit
GACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCG10.5988023952095809No Hit
GTGTGTATGTGCTCAGCTGTGCCTTGATTTGTTTCAGAAATGCAGGGACT10.5988023952095809No Hit
GTCAGACTCTGTAGCGTCGAGGACTTCTACTCGCCATCCAAGTTTGTCGA10.5988023952095809No Hit
GTGAATCCGCGGATGGCGGCCTTTGCGGCGCAGTAGGCCGACTGCAGCGG10.5988023952095809No Hit
CTCTTAGTGCGTGTAGCTCTTCTGGCTGGAGGATGTGGTGGAGACAAACT10.5988023952095809No Hit
GCGACACGACGAGGTTGTCGTCGTCGACGTAGCGCGGCGCCTGGCTGGTC10.5988023952095809No Hit
ATAAAAAGGGATGGGTAATGTAAAAATCTGAATCAATATGCTAGTTCTGG10.5988023952095809No Hit
GGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGT10.5988023952095809No Hit
ATATTACAGGGGGGTTGCCTCGTGTGGCAGAACTATTCGAGGCTAGAGTT10.5988023952095809No Hit
GTCTTTAGCAACGTCCATATTGTAGAAGACGTGCTCGTTGGGGCCTTCAC10.5988023952095809No Hit
TTCATCGACTGGTTCGCCGACGGATCCGTGTACGTCGTCGACCTCGCGCT10.5988023952095809No Hit
GCAACCTCCGCTTCCCGGGTTCAAGTGATTCTCTTGCCTCAGCCTCCTGA10.5988023952095809No Hit
GGGTGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAGTAAAATT10.5988023952095809No Hit
CGGCGAAGAAGAAGCAGAAGCAGCTACTGAAGAAGCTGCTAGCGAAGCTG10.5988023952095809No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG10.5988023952095809No Hit
CCATAGAATGGAATCGAATAGAATCACCGAATGAACTCAAATGGAATCAT10.5988023952095809No Hit
CGTTTTAATGCTGGAGATGGAGCCTGAGATGTAAATTAATAAACCACACC10.5988023952095809No Hit
ATGTTTTGGTATGCGAAGTGTAGGTTAAGTTGCAAACCAACTGCTAAAAA10.5988023952095809No Hit
TGCATGAAGTACTTGTAAATGCAGGCTCCACCAACAATAAGTCCTGCCAA10.5988023952095809No Hit
CTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGC10.5988023952095809No Hit
TCCCAGGCTGGAGTGCAATGGCGCAATCTCGGCTCACTGCAACCTCCACC10.5988023952095809No Hit
GTGTGCCCACAGGGCCATGGCAGCAGATAGGCTTAGCCATGGCCCAGAGG10.5988023952095809No Hit
GCCACGAGAGCATACCGGGAATACCCATGATCTCATCGGCATTCTCGCAG10.5988023952095809No Hit
CACTAGGGATAACTGGCTGGAATCGAGGGCGGCTGGAGGACATTCTAGCA10.5988023952095809No Hit
GGAAAGAAAGAGCTCAGTGGCTGGCTGAAGAAGCGCGCGCAGGATGCCGC10.5988023952095809No Hit
ATTCAAATCAATCGCAGTACGTTGTATTACAAGCCCAAAGAGATTTCATC10.5988023952095809No Hit
CCTTATTTCCACCAGGCGGCTCGCTGATGGTACAAAGCCTCTTCCGCCAC10.5988023952095809No Hit
GCCTATAACGCAACACAGGCACTTGCGTCACTGCCGAATTCCTACCCGGT10.5988023952095809No Hit
GAATGGAAAAGAATGGAATCAACCCGAGTGGAATGGCATGGAATGGAATG10.5988023952095809No Hit
GCGCATGCTCCAGCTACTGATGTCACGCAGGCAGAGCAGCAGTTGGGGGA10.5988023952095809No Hit
GACAATCAGGATGCGCTCGTTTGCCATGGGAATCGTGTTTCAGTTCTGAA10.5988023952095809No Hit
GGATTAGTTAGCTTTCAAAATGCATATGGTATGTGAAGAAAAAAAAAGCT10.5988023952095809No Hit
GCCTAGAAGTAGTAACTTATGATAGGCAATAACCACATGTGGAAGAATTA10.5988023952095809No Hit
GCATTTATGGCTACAATTACTTTGGCATGTTTTTAGGCCCCTCAGATATA10.5988023952095809No Hit
CAAAGACAGGCATGACAGAAAACTGCATCGCGGGTAGTTTAACCAAAGCC10.5988023952095809No Hit
GACTACGAGTGCGTGACCGTGGGCGGCACGCAGCGGCTGCTGCGCGAACT10.5988023952095809No Hit
GCCTCAAACTCTTGGGCTCAAGCAATCCTCCCACCTCAGCCTCCCAAAGC10.5988023952095809No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT10.5988023952095809No Hit
ACGCGACCCCGTGCGCAGTTCCTCCAGACACTACAACGATGTATCCGTCC10.5988023952095809No Hit
CGCCGAAACCACCCCCGCCCTCGAAGCCGCCGGCCTGCAAGCCGACCTAC10.5988023952095809No Hit
GGGTTTATTTGAGTCCTACAGAGTTCCCAGAGTGGCCAGCCGCAAGAGAA10.5988023952095809No Hit
GTACTTGCCCGAGTCCTCGGAGGTCACGCCTTTGATGGTCATGCTGACGT10.5988023952095809No Hit
ATTTCATTCCAGTCCATTCCATTCGAGTCCGTTCCATTCCAGTCCATTCC10.5988023952095809No Hit
GCCTATATCTAATGCCTGTTTCAGGTAAGGGTTATTCAAAGATCTAGTTA10.5988023952095809No Hit
CACGTCAAAGGGTTTGCTATAATGACAAGAAATTACAGAGCCGCAATATT10.5988023952095809No Hit
CCTCCACGCCTCGGCGCTCATACGGGCGCTGCGGGCTGAGGATCCAGAGG10.5988023952095809No Hit
GTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTT10.5988023952095809No Hit
GGATTGGAGTGGAGTGGAGTGTAATGGAGAGGAGTGGAGTGGAGTAGAGT10.5988023952095809No Hit
CGTCATCAACTACCCGGCCGTGCGGCGCTACTACATCACGCGCAATGCGC10.5988023952095809No Hit
AAACAGAGCCCAAGGAGTGTCATGGCAACACCGCTCACAAGCTGCCAAAG10.5988023952095809No Hit
AGCGGAGGAGGTAATGATGAACGTGCTGACGAATCTGCAGGCGTGGTGGA10.5988023952095809No Hit
ACCTCAGCCTCCACTCCTCTTCTTGTTTTAGACTTTATGCACAGCTTCAA10.5988023952095809No Hit
GGTCAAGGGGGCTGACGTTCCCCAGGCCGTCGTTGAGGGGCTGAGGTCTA10.5988023952095809No Hit
TTACTAAGTCACGTTGGTCTGAGAGCCACAGTTCGTGGACACTCAGCCAA10.5988023952095809No Hit
GGAGAATCACTTGAGCCCAGGAGATGGAGGTTGCAGTGAGCCGAGATTGC10.5988023952095809No Hit
CCTTATAGAGGAAGTCCTCGTGAACCGGGTTGCCGTCCTCATCAGCGTGG10.5988023952095809No Hit
ACCTTTTCAAGCCCGGCCTGGCGCAGGGTTCGCAGCCCTTCAGCTGTCTC10.5988023952095809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)