FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCACGGGGCAGGGCTGACCTGCTACGCGCTGGCCATGACCGTGCTTC42.9197080291970803No Hit
ATATTACAAGGACATGTATCCTCTGTAGTCTCACCCACGTTTCCCAGGTT32.18978102189781No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG32.18978102189781No Hit
CTACAGAAGTCTGCACGCGGCGAGTATGAGATTACGGATGTCAATCGCCA32.18978102189781No Hit
GATTCCATTCGATGATGATTCCATTCAAATCCATTCGATAATTCAATTCA32.18978102189781No Hit
ACGTACTCGTGCTGGTGTGAGCCCATTTACTCCCGATAAAATGCATCTCC32.18978102189781No Hit
TCCTGGGGGTGCTCTTTCGAGGATATTTGGGCTGCCTCCGGAGTCACAGT32.18978102189781No Hit
GACTACGAGTGCGTGACCGTGGGCGGCACGCAGCGGCTGCTGCGCGAACT32.18978102189781No Hit
ACGTATTGGTGTAGCGAAGCAGATTCCGCAGTTTGCGCATCTTCCCTTCG32.18978102189781No Hit
GAACAGCGCGCCGGCCGGCAGGGGGTCGTAGAAGAGGTGGCAGGTGCCGC21.4598540145985401No Hit
CCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCA21.4598540145985401No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG21.4598540145985401No Hit
GAGTAAGGCATGGAAACCACTAATGATAGAAGAGTTAGACAGATGAACTT21.4598540145985401No Hit
CCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAG21.4598540145985401No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA21.4598540145985401No Hit
GCGTGGATGCCGTTAATGCACCCCATGACGTTGACGTCGAAGAGACGCTT21.4598540145985401No Hit
GGTCCATATATCCACTTGCAGACTTTACAAACAGTGTGTTTCCAAACTCC21.4598540145985401No Hit
ATATTACAGGGGGGTTGCCTCGTGTGGCAGAACTATTCGAGGCTAGAGTT21.4598540145985401No Hit
TGTATGTGGTGCTGCCTAGGAACAGTAGGTATTTTTGTGGAGCAAGGGAT21.4598540145985401No Hit
CTGCAGGAGTTATGAAGTTTTGTTTTCTTTTTTTTTTTTTTTGAGACGGA21.4598540145985401No Hit
CATAAGGAAAGGTAAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG21.4598540145985401No Hit
GAGGCAAACTGGGTGATTTTTCCACTGGCTGACCTTTTAGAAGTTCCAGA21.4598540145985401No Hit
GCTTTGGTGTTTGCCCCGACAACGCAATTACCAAGATCAAACCGGGCGAA21.4598540145985401No Hit
CATTATTAATACACCAAAGGGTGATTCCGGTTCTCGGGAAGACGGCTATG21.4598540145985401No Hit
GTGATGTTTGTGTTCCACTTCAAGAATTGAACTTTCCTCTTGACAGAGCA21.4598540145985401No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA21.4598540145985401No Hit
GATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTC21.4598540145985401No Hit
GCAGGTACCTTACTTGGGATTTACTATACCACAGGGGTCCGAACGCAGCC21.4598540145985401No Hit
CCCCTGACTAAGGCCACCTTGCAGCCGCTTTCCAGGGCCACCAAGGGCTC10.7299270072992701No Hit
TTTGACAGTAGACTGATTAATATAGCAGTTTGGAACCTGGATGAGGGGAC10.7299270072992701No Hit
TTTCTTCATTTATCAGTGAAAGAAGTTAGAAATTAACTTCCCAAAAAAAT10.7299270072992701No Hit
CTTTTAACTTGGCATTATCTTTTTCAAGCTTTTGCTTGTGATCTTGCATC10.7299270072992701No Hit
CCATTCTAACTGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATT10.7299270072992701No Hit
CTTCACCCTGGGCAACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAAAAA10.7299270072992701No Hit
GATTCACGGATTCGCTGCGCTGCGAACTGATCCGCGACGGCTCGAAAGTG10.7299270072992701No Hit
TTCATAGAGCAGGTTGGAAACACTCTTTTTGGAGTATCTGGAAGTGGACA10.7299270072992701No Hit
TTTGAGACGGAGTGGCCCAAGCTGGAGTGCAGTGGTGCAATCTTGGCTCA10.7299270072992701No Hit
CCATGTATTCCCTGAACTTGAACAGGGACATGATAATGCTTCAGATCTCC10.7299270072992701No Hit
CTACTTCCCAGGCTCAAGCAACTCTCCTGCCTCGGCCTCCTGAGTAGCTG10.7299270072992701No Hit
CTACTGATTTTTGTACATTAATTTTGTATCCTAAAATTTTACTTATGTTG10.7299270072992701No Hit
GCCTCCGCCTCCCAGGTTCAACTGATTCTCTTGCCTCAGCCTCCCAAGTA10.7299270072992701No Hit
CGTCATCGCCCGTACCGACGCCGAGGCTGCGACCCTCATGACCTCCGACA10.7299270072992701No Hit
GATAAAATTTACACGATGGAGCCCTATACAACAATGGAAAAGAAAGTGCT10.7299270072992701No Hit
GGAGCATTCTCAATCATTAGTTGGGTAGCCCCATCAAGATCTAAACTTGA10.7299270072992701No Hit
ATACGATAATATTTCGTTGGGTCTTCTTTAGTTCCACTTTCACGACCTGA10.7299270072992701No Hit
GAACAGCGCGCCGGCCGGCAGGGGGTCGGAGAAGAGGTGGCAGGTGCCGC10.7299270072992701No Hit
GGTAAAACATATGGACCAGAGCTTAGAGGACATCAACGTCAAAACGGTAA10.7299270072992701No Hit
ATTAACACTGACAACTTAGGCCAACAGTAGCATTATCTTGTGTCAACTGA10.7299270072992701No Hit
GTTGACACCATTGTCAAGCCAGTCCTCGAAGGGATGATTAAAGAAGATCG10.7299270072992701No Hit
CTTTACCACTAAACGATGTGATAAATGCCAAGGGAACAATGCATCTTTTG10.7299270072992701No Hit
GATAAAATCAGCCATTCCAGCAAAACTAGCTACTTAGTGTCTATCTCTCC10.7299270072992701No Hit
CCACTCAAATCCACTCCTCTCCACTCCACTCCACTTCACTCCACTCCACT10.7299270072992701No Hit
GATCAAGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTG10.7299270072992701No Hit
CACCAAAGCAGTTATCGCATCCGAAGCAGTTACCGCAGGACATGCAGCGA10.7299270072992701No Hit
CAGGTGTTCAAGATCAGCCTGGCCAACATGGCAAAACCCCATCTCTACTA10.7299270072992701No Hit
GTGTAACCTCTCTGAGGTGTTGGCATTGGAACTGAGAGGGAATGACATAA10.7299270072992701No Hit
ACACATTTCAAAATGTAAAAAGGCATTTCGTGAAGGATCTAGGAAGTCAT10.7299270072992701No Hit
GTGATGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGGGCCTGAT10.7299270072992701No Hit
AGCTCTCACACTGCTGACTGATGCTCACATTTTTTTCCTTTTGTTCTGTA10.7299270072992701No Hit
CAGAAGAAGACAAGAAAATGTGGGAAAGTTTCGAACTTCCTAGAGACTTG10.7299270072992701No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT10.7299270072992701No Hit
GGCAATTTTTGCACAATTGATTTTTTATCCTGAGACTTTGCTGAAGTTGC10.7299270072992701No Hit
GAATCAAATAATCGTTGAATGGAATCGAATGGAATCATCGAATGGACTCG10.7299270072992701No Hit
AGCCTGTCTTGTGACACTGGAGAGGACAAAGGAACAGCTGGCAAAAAGCC10.7299270072992701No Hit
GGTTTCTGAAGGACCCCCTGTTCTTTGCGGAGAGCACCTTCTTGAAGCGG10.7299270072992701No Hit
ATATCTGTCTATTTTTGACCTAGGAGTACCATGTTCTATTAGCCACCAAA10.7299270072992701No Hit
TCTTTTGTTAGCAGCTGGGCTGACCTCGCCTCAAGGCTCTTTTTCATTAT10.7299270072992701No Hit
AATGTGGCTTGAAAAATCCAGCAAGAGCGGGGACCGCGGGTCCCGAGGCG10.7299270072992701No Hit
CAACTATGCGCAACTTCGCCAACGGAGAGATCTTCGTTCGTTATGAAGAA10.7299270072992701No Hit
GACTCCGAGTGAGTTACCGTGGGCGTCACGCAGCGGCTGCTGAGCGAAAT10.7299270072992701No Hit
GAATGGATCAACACTGTTAGTATCAACTAACAGTGTTGATCCATTCTTTT10.7299270072992701No Hit
GTCTTTAGCAACGTCCATATTGTAGAAGACGTGCTCGTTGGGGCCTTCAC10.7299270072992701No Hit
GCAGTACATAGCTAACGTTGGCGCCGCTCACGGCGGTGAGATGGGTGAGG10.7299270072992701No Hit
CCCGACAGCCTCCTCTGAGGTGGTCTGGCCCAGCATGGCTCCCCCAATGG10.7299270072992701No Hit
CGATAATAAAGATCACATTTTTCATTTAACACATCCCTAGGACACGTAAG10.7299270072992701No Hit
ATTCTCTGCCGGGGCAGTCCGCTAATGCCCCGGCAGAGAATACACGCAGG10.7299270072992701No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG10.7299270072992701No Hit
GAAAACATACCTGACTTTTCAGACATCCTTCTAATTCTGCAATGAACACA10.7299270072992701No Hit
GTTCAACATGGTGAAACCTCGTCTCTACTAAAAATACACAAATTAGCTGG10.7299270072992701No Hit
GATAAAGAGCTAATTAAAAAGATTATTGCCTGTACAAAGTAGTTGGGAGA10.7299270072992701No Hit
GCTTTCTACTGCATTGAGGAGTATTTTGCTGGGAATTGAATATTAAAAAT10.7299270072992701No Hit
AGCCGACGCTCCTCCGAAAGCTCATTATCGTCCGCTAACGCTCGCATATG10.7299270072992701No Hit
CTAGAACTAAATTGAAAGGAAAACCCTAACTTTCCACGCCTAAGGGAAAA10.7299270072992701No Hit
GAATAGACCAATAATGAGTTCTAAAATTGAGGCAGTAATAAACAACCTGA10.7299270072992701No Hit
ATCGTCGCCATGACTATCCTTGTCGGAATTTCCACCGTGAGCGTCATCGC10.7299270072992701No Hit
GCCTCAAACTCTTGGGCTCAAGCAATCCTCCCACCTCAGCCTCCCAAAGC10.7299270072992701No Hit
ATTCCATTCCACTCTAGTCTATTCCACTCCATTCCTTTCCATTCCATTCC10.7299270072992701No Hit
TTCCTTCAATATGTTTTAAGAAATCGGTATCTGCTTCTTGGCCAAGAGCT10.7299270072992701No Hit
ACATAATGACAGCACACGCGTGTACACCCGCCTGGCGAACCATACCTTCC10.7299270072992701No Hit
GACTACGAGTGCGTGACCGTGGGCGGCACGCAGAGGCTGATGCGCGAACT10.7299270072992701No Hit
ACCTCTGCCTCCCGGGTTCAAGCGATTCACCCACCTCAGCCTCACAAAGT10.7299270072992701No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT10.7299270072992701No Hit
AACGTAGGCTGGGCGGGGTGGCTCACGTCTGTAATCCCAGCACTTTGGGA10.7299270072992701No Hit
CCATTCATGGAGGGTAGAGATATCGACATCCTTTTTCCCATCACTCCTGA10.7299270072992701No Hit
GTTGTGCAGCCTCAGTTGGCTACTCCGGGCAAGTTTCCATTTTCTGAACA10.7299270072992701No Hit
ACCATACCCTCCTCAAGTTTCATCAAGGGAAACTTCATGCAATACAAGGG10.7299270072992701No Hit
ATGTTTGTGTCTCCCCAAAATTCATGTGTTGAAGCCCTAACCTTTAATGT10.7299270072992701No Hit
CCCATTTACCTGCCTCGCATCCCATCAGCTGGGCGGGAGGGCAAGGCCAA10.7299270072992701No Hit
CTCTCAGACCACAGTGCAATCAAACTAGAATTCAGGATTAAGAAACTCAC10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)