FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length146
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTGCCTTCGCGCGAAACCGGCTTCACGCCGATGCCCGACGTTTCCG32.0689655172413794No Hit
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAG32.0689655172413794No Hit
CTCTAACTGCTTCCTGCTGAATTGGGGCATAGTAGGGGTCGTGCAATTGA32.0689655172413794No Hit
CAATTTATTTTGATTTCACAGACTCACAACTGGAGGAAAATTTGATTCAG32.0689655172413794No Hit
GAATGGAATCGTCATAGAATGGAATCGAATGGATTCATTGAATGGAATCA32.0689655172413794No Hit
ACATTCCTTTTGATAGAGCAGTTTGCAAACACTCTTTTTGTAGAATCTGC21.3793103448275863No Hit
GGATGATGATGAGTTCCCTGGGTTTTCTTTTGGTCTTGTATATCCCAGAC21.3793103448275863No Hit
ATCTGAAAGTGCATAATTGCAGCACTTTGAGGCTTAAGGTAGAAAAGGAA21.3793103448275863No Hit
TAGTTTCACATGGCTTGGAAGGCCTCAGGAAACTTACAATCATGACAGAA21.3793103448275863No Hit
GTATCAACGCAGAGATGGTTCTGAAATGTTAGACAATTTGAAGTTGGGAA21.3793103448275863No Hit
ACTCCACCCCTCCCGTTCTGCAGGGAGCTCCTTCTCTGAGCTCTCACTAT21.3793103448275863No Hit
GCGTCCTACCAACATCAGCGCCACGCCTCTCCCATTGCGCAACATGACGG21.3793103448275863No Hit
GTGTTAGTGTTTTCCTATATGTGGGCATTTAGATTGCTTCCAGTCTTTCT21.3793103448275863No Hit
TCCCACTTGCCCAGAATGATCTCAAACTCCTGAGCTCAAGGGATCCTCCC21.3793103448275863No Hit
TTATAGCACCAAAGCCTGACAGGTAAAGTCTGCTGGCTTCACCGGGGCAC21.3793103448275863No Hit
ACCTCACCCAGCCCTCTGACATGGGTATCATGATTGTCATTTTGCCAAAG21.3793103448275863No Hit
GATGTATGTTTTGCAAATATTTCCTCCCATTCAACAGATTATCTCTTCAC21.3793103448275863No Hit
GTTTATTTTGGTTATGACATTTGAACTTTAGTTGACAGACTTAGCTTATG21.3793103448275863No Hit
CCTGATCGCTGAAGTTACGAAAACCAGTTCTCTTCTCAAGGCTACCAAAG21.3793103448275863No Hit
CTTTTGAACTTCTTAAAATGTGTTTTGTGTCCCCAAATGTAGGCAATTTT21.3793103448275863No Hit
CTCATACACTGTTTGTGGAAATGTAAATTAGTACAGCCACTATGGAAAAC21.3793103448275863No Hit
TCCTACCTGGCCTGTTGTGATGTAGTTCCTACCTCGGTTTGCTGTGGTGT21.3793103448275863No Hit
ATTCAATTCCATTCAATAATGATCCCTTTCGAGTCCATTCAATGATTCCA10.6896551724137931No Hit
GTCTTGAACTCCAGACCTCAAGTGATCCGCCAGCCTCCACCTCCCAAAGT10.6896551724137931No Hit
GTGCTGAATTCCGCAAGTCTCGGCCTGGACACCTTCTTCGAAGGCGTTGG10.6896551724137931No Hit
GTTCCGTCCGACGCCGTGCCGTTGCCGCCGTTGCCGCCACCGCCGCCAAC10.6896551724137931No Hit
GTTTGGAAAGACTCTGTCTGTAAAGTCTGCAAGTGATTAGTTAGACCCGT10.6896551724137931No Hit
TTCTAGGAAAGTTATCTACAAGTAGGGATAGTTTGACTTCCTCTCTTCCT10.6896551724137931No Hit
ATCTCTGGCATGGTTTATGACTACTGAGCTAGCAAATGCATGCTTTTCCA10.6896551724137931No Hit
CTTCACCCCAGCTCCATTCCCCATTCTGGGTAAATGTATAAAAATCCATA10.6896551724137931No Hit
GAATAAGGTCCAAATCCTTTAGATAGATATCCAAAGGCTTCCAGAATCTG10.6896551724137931No Hit
TACTCAGAATCCAAAAAGCTGACTTCTTTTCTGGATGCATCACTCCTGGC10.6896551724137931No Hit
TTCCCAGTGATTCAGATTTATTAGGGACTTAAGAATCTTTTTTTCCCTTT10.6896551724137931No Hit
CACCCTATTTGTGCAGTTTCCAGTTGGAGATTTCAATCGCTTTGAGACCA10.6896551724137931No Hit
ATTTGGCTGTGAATCCATCTGGTCCTGGACTCTTTTTGGTTGGTAAGCTA10.6896551724137931No Hit
TACTAAGAGAGCACATAAAAAGTAACAAACAATAATTCCACCAAAGGAAG10.6896551724137931No Hit
GAATGGAGCAGGAGGAGTAGAGGAGGAGAAGAGGAGAAAAAGGAGAAAAG10.6896551724137931No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTGCG10.6896551724137931No Hit
TGAGAGGAATATGAGGCAAAATCCAATCTACTTGATTCCACAGGTAGATT10.6896551724137931No Hit
AGTAGATACTCTGTGAGAGAGAGTTACACATCACTATAAATCAGTAACTG10.6896551724137931No Hit
GACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAA10.6896551724137931No Hit
CGTCAAGGGCGTGCCCATGCTCTGGGTCTGGGGCGACAACCTCGGCAGGT10.6896551724137931No Hit
CTGGGCTGAAGCAATTTTCCTGCCTCAGCCTCTCCAGTAGCTGAGGCCAC10.6896551724137931No Hit
CATTATAACTAGATTGGTGTGAGGAGCACAAGGCCTAGTAATTGCAAAAT10.6896551724137931No Hit
TTGCTCTTGATTCACCTGGCATGCGTGTGAGGCCTCCTCAGCCATGTGGA10.6896551724137931No Hit
CCTTGTAGATTCTGGATATTAGATTTTTGTCAGATGGGTAGATTGCAAAA10.6896551724137931No Hit
GCCAAATAACATAACATTAGGAAAAAAGTGATGAAACACAATAATAGCAG10.6896551724137931No Hit
CTCACTTGCTTCGTAGAGTCCAGCGAAGGCCCGAGCATCGCGCGTGACGA10.6896551724137931No Hit
CTACTCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGT10.6896551724137931No Hit
TTCCATGCGATTCCATTAGATGATGACCCCTTTCATTTCCATTCAATGAG10.6896551724137931No Hit
CATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATC10.6896551724137931No Hit
GCCAAGTGGAGGGCAGGAACAGTGAGGGGAGGGGGACATACTGAGTGACG10.6896551724137931No Hit
AGAGGAGAGAGTCTCTTTCTAAGATTAGCAGCGGGAGGCTGAGGACAGGG10.6896551724137931No Hit
TTTCATCACACAGCTGGGTTGGTTCCACTATGTCTTATGTCCAGTATCTT10.6896551724137931No Hit
TACCAGTACTTGTTCCAGTGGAGGTGGCAGGGGTGGACTCTGTGAGGTTT10.6896551724137931No Hit
GGATTCACAGAAGCTGGATTTGAAATAAATTCTCTATGTCGTCCAGCCTG10.6896551724137931No Hit
CTCAAACTCCTGACCCCAGATGATCCGCCTGCCTCGGCCTCCCAAAGTGC10.6896551724137931No Hit
GCCCCGCCTGCATCTGGTACGGCCACACCACGAGCTGGAGCGCGCTCGAC10.6896551724137931No Hit
CCCTATGCTTCAGCCAGGTGAGGGTTTTTGTTGTGTGGTGAAAATTGGTT10.6896551724137931No Hit
GTTTATTTATGCCAGCCAGTGTGTAGTCCTTCTTTAGTTTAACATTCTAA10.6896551724137931No Hit
ATTTTGACAATCATCTCTGTGTATATGTGCATTTGCATACAAAAAGTCAC10.6896551724137931No Hit
GCGATGATCGCCTCGGCATCCGCCGCCACCGCGTCGAGCGCGCGCGTGCT10.6896551724137931No Hit
GCACCAGAACGCTGCGCGAGCGGCGGGCGGGCACCGCAGCAGCGCCCGGA10.6896551724137931No Hit
GTGTGTGTGGAGGGGTAAACGTACCTCTTTTTGTTGTTGTTCCTGAGGGG10.6896551724137931No Hit
CTCAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAATCTCACTC10.6896551724137931No Hit
TAGTTTCACATGGCTTGGACGGCCTCAGGAAACTTACAATCATGACAGAA10.6896551724137931No Hit
ACAGAGGACTAGCGTTGACCTTATACCAAAGTTGGCCAAGTGGGATTATC10.6896551724137931No Hit
CCATAACATTTGAGTCCATTCAATTCCATTCCTTTCGTTTCCATTCCATT10.6896551724137931No Hit
ATGCTGTAGATTTCCCTCTATTCATTGCTTGCTGCATTTCACAACTTTTG10.6896551724137931No Hit
GTGCCCTCATTCCTCTCCAGTGAAGGTCACAACCACGTCTTCAAATGATA10.6896551724137931No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTGCGTTTAGCATCGCGTA10.6896551724137931No Hit
CCTCTGAGATGAATCCTGCCAACCTGAGCTTGGAGACAGATTCTCTCCCT10.6896551724137931No Hit
AGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGA10.6896551724137931No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAATAG10.6896551724137931No Hit
GTCAAGAACAGACAGAACAAGCCTCTCGTGCCAAGCACCCAGTAATTTAG10.6896551724137931No Hit
TACCATTCCTCTCCATTCCATTCGAGTTGAACCCATTCCATTCCATTCCA10.6896551724137931No Hit
GTCTCAGAATCTTCTTTGTGATGTTTGCATTCAAATCCCAGAGTTGAACT10.6896551724137931No Hit
GGGGAATGTAATGGAACGGAATGCAATGGAATGGAATCATCCGGAATGGA10.6896551724137931No Hit
AAGACCTAGACCTCGTAGGGTGATCTAATCATCCCTGTTTACCTGTGACT10.6896551724137931No Hit
GACTAACAGCGGATCTTTCGGCAGAAACTCTACAAGCCAGAAGACTGTGG10.6896551724137931No Hit
CCATTCTCTGCATGTTGGGATTTGGGAGCTGCTGTGGCAATTGTTGGGCA10.6896551724137931No Hit
GTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAACGA10.6896551724137931No Hit
TCAGGGAGGATCAGAGAACATGCATTAAAGTATTCTTTGGTCAACAATAA10.6896551724137931No Hit
GTTCTAAAGCACAAACACTGCTTTAGGATGCTGGCGAACTCCATGGCAGC10.6896551724137931No Hit
GCCGAGAAAATCACACATGCCAGCTTCAAGGCCGAAAGTGACATGGGCCA10.6896551724137931No Hit
ATGACTTAGGGTTTAAAAACTTTAACCTTTACTTTTCTGTTCTTGTTTGT10.6896551724137931No Hit
GTTTACTATTAGGGTGGCTGGTACAATAAAAGCTCAAACTTCACCACTTC10.6896551724137931No Hit
ACACCCGGCTAAGTGTTGTATTTTTAGTAGAGACAAGGTTTCACCATATT10.6896551724137931No Hit
GTATTAGACAAATCAATGAGAAAGAAAATTAACAAGGATATTCAGGATTA10.6896551724137931No Hit
GCCACTAAGAACATTTCAAATAGGTATACTGAAGCAGAAATGTTTTTCAT10.6896551724137931No Hit
ACCAGTAATGGGATTGCTGGGTTGAATGGCAGTTTTGCTTTTAGCTCTTT10.6896551724137931No Hit
ATTTTAAAATTACTGTTTCAGTCTCTCTGTTTGTTGTTGGTCTGTTCAGG10.6896551724137931No Hit
GTGTAAGGGAATTGAATAGAATCAATCCGAATGGAATGGAATGGAATGGA10.6896551724137931No Hit
CTTTTAAGGGAAAAAATTGATTAAAGGTGATATCTTTCCATAGAGGGCTA10.6896551724137931No Hit
ACCTCCACCTCCTGGGCTTACCTCCCATCTCAGCCTCTCAAGTAGCTGGT10.6896551724137931No Hit
GTTCTACCTCAGAACCTTTGCACATGCTGTTCTCTGCCTGGAATAGTCTT10.6896551724137931No Hit
TGCCTGGAGAGGCTGGGACTGACAGCACCAAGTCCCTAGACTGCACACAG10.6896551724137931No Hit
TTTCTGAGTAGAGAAATGATCCGTAATATAGGCCTCGCCCGATGTGTAGG10.6896551724137931No Hit
GGTTCGTCTCACAGATGCCTGATCTCATCTGTGGGGAAATTCTAGAATTG10.6896551724137931No Hit
CCTTAGTTCAGGCCCTCATCATTTTGCATCAAATCCAACCTGGTTTTTTA10.6896551724137931No Hit
ATCTCAACTCACCGCAACCTCTGCCTCCCGGGTTCAAGTGATTCTCCTGC10.6896551724137931No Hit
CTTTATGATGCTCTGCTAATGACAGGGATGAGACTGAAATAAAGCCTCTT10.6896551724137931No Hit
ACCATGGCCACTTCGTGCTTCAAAGGTGTAGTCTAATAGCTCAGCTGTAT10.6896551724137931No Hit
AGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGA10.6896551724137931No Hit
GTCAGAGAACGCATCAAAACACGAATCGAAACCTTCGTCTTCTGCCGCCT10.6896551724137931No Hit
GGGAAGATATTTCCTTTTCCAACGTAGGCCTGAAAGCTCTCCAAATATCC10.6896551724137931No Hit
CCCTGAGGCTGCTGGTGAATAATCTTTGCAAATTTTTTCCCATTTTCAGA10.6896551724137931No Hit
GACCCACAGATAGTATCATACATAATGAGGAAATTCTGAAAGCCTTTCCT10.6896551724137931No Hit
TGATAGATTTTTTTTTTTTTTTTTTGAGACAGAGTTTTGCTCTTGTCTGT10.6896551724137931No Hit
GCGCTGGCTTTAGCCGCCTAAGCTGCATGGTATCCTGAAGATTGTCTTGT10.6896551724137931No Hit
ACTCTAGCCAACAACAGATAGATTCCGTGTTCTCTGCCTAGTCTTTTTGC10.6896551724137931No Hit
CTGTGGCTGATTCTGGGCCAGTATCACGAGCTGAGCACAGCCTGCCAGGC10.6896551724137931No Hit
TTCCAATCCATTCGATTCCATTCGATTCAATTCCATTTGATTCTCTTTCA10.6896551724137931No Hit
CTGCTCCTCGCACCTCTTTCCAGACCCTTCTTCCTCTTTTGAGCAGAGCA10.6896551724137931No Hit
ATTCTGCTATTGGTAGGCCTGGAAGGCAAGGTCAGAAGTAGCAAAAAATG10.6896551724137931No Hit
ACATATGCCTTACTTGTCACATAAGTATGTGTATGCATTTTTGAACATGA10.6896551724137931No Hit
GCANTTTATTGGAGAGATGATATCAATGTAAAGGATGATCAAACACTGGA10.6896551724137931No Hit
AATATCCACTTGCAGATTCTACAAAAAGAGTGCTTCAAAACTGCTCTGTC10.6896551724137931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)