FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length146
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGTGCCTTCGCCGGTCAAAGAGCCGGAGAAGACCGACATGGTCATCT32.0689655172413794No Hit
TCCATAATCCTCACATGTTGTGGGAGGGACCCAGTATGAGGTAATCGAAT32.0689655172413794No Hit
TTCATTGACTGTTCCATTCCATTCCATTCGATGATTCCATTCGAGTCCAT32.0689655172413794No Hit
ATACATTTCAATCCCTGTATCTTTAACCTCCTTGTTAAGTTTGTCTCTTC32.0689655172413794No Hit
CTTGTGAGGCTCTCCTTGCTGTGTCATAACACGCCAGAGGGCATTGCATG32.0689655172413794No Hit
CAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACA32.0689655172413794No Hit
CCCATTAGGCACCCGCACTCTGACACTGGGGATCAAATTTCAAGATGAGG21.3793103448275863No Hit
GAGGTGCTCTATGGACAGAGGGCCTAGGATAGAGGACAGAAGAGGACCAT21.3793103448275863No Hit
ACCTTACACCATATACAAAAAATCAACTCAAAATGGATTAAAAAACCTAA21.3793103448275863No Hit
ACATTAATCAAAGGAAAGTTAGAGTAGCTATCTTAATTTTAGGCAAAGCC21.3793103448275863No Hit
GTTACAAGTGTATGCATGGGGTTTTCAAAATGCAGCTTTTAGGATTCAGG21.3793103448275863No Hit
GTTAACATTCTCCATTGACGTCTGCTTCTAATTTACAGAATGTTGATTTT21.3793103448275863No Hit
GCTACTTAAATGGGAAAGAGATTTAGTACAGGGAATTGGGTACCCTTACA21.3793103448275863No Hit
GCTTAGACAACCATGACAAAATATCTTAGATTGGGTAACCTATAAACAAC21.3793103448275863No Hit
CATCTATTGATGGACATTTAGGTTGCTTCCAAATCTTAGCTATTGTGAAC21.3793103448275863No Hit
CACTTGCAGAGTCTACAAAAAGACTGTTTCAAAACTGCTCTATAAAAGTA21.3793103448275863No Hit
GGCAACCTGCCACTCGTCCCGATCGGAAGGCTGGGTTCCGACCCGCTTGA21.3793103448275863No Hit
ACCGTGCCTGGCCCTTGGGACTCTTGAGTGGCACTTGGTGATGCTCACCA21.3793103448275863No Hit
TATTTCACATACGAAAAAAGGATACTTTTTCACACAGATGAGATGGGCAA21.3793103448275863No Hit
GTATTTTGCTCTCTTTATCTTTTAATTCTCCAATTTCAAGCACATAGGAA21.3793103448275863No Hit
GCCTAATTCTGTGGCCATTTCTGTGGTTCCCCATGCCTAGCGCAGTGCCT21.3793103448275863No Hit
CTGTCTAGGTTTGATGTGAAGATATAGACGTTTCAAACGAAGGCTACAAA21.3793103448275863No Hit
GTAATGGATTCATCGCATAGAATCGAATGGAATTATCATCGAATGGACTC10.6896551724137931No Hit
GCATAGTCCATTACGTACAATTAACATGATGTTATCAATACAATGAACCA10.6896551724137931No Hit
GCATTCAACTCATAGAGTTGAACACTTCCCTTCATACAGCAGGTTTGAAA10.6896551724137931No Hit
GCATTATCAACAATAATTTCCTAAAGAGATAGAAAGCCTTTGTTTATCAG10.6896551724137931No Hit
GGGTTGTTATTTACAATCTGGATGGCTTCATCCAATGTTTCTGTCTCCAG10.6896551724137931No Hit
CTTTGGAATGGTGTAGTATGCAATGCAATCGACTGGCAGGGAATCAAAAG10.6896551724137931No Hit
TCCACCTCCTCCTCCCCTCATACTCATCCTCTCCCCCTACTCCTCCTTTC10.6896551724137931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACATGCTATCAGGTCCTAAAAAAGAGAAAAGGTTACAGTCAAACTCT10.6896551724137931No Hit
GTTCATATCTGTCTCATGTCAACAATATATTTTGGGCGAACAATTCTCCA10.6896551724137931No Hit
CTTGTTAGTTGTTTTCTGAGAGGAGTAGTAATGAATCCAGTTGCTTTGAC10.6896551724137931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6896551724137931No Hit
CCACTTTTCAAGTGCTTAATAGACACTTCTGCTTAGTGGCTACTGTTTCA10.6896551724137931No Hit
CTTCACAACAGCCTCACCCATCACAGGCTTGGAGGCCTAGGAGGAAAAAA10.6896551724137931No Hit
GTGTCACCTTGACATCCTGAGTGATTCCAGGGCTTCAGAAATAAGTTGAA10.6896551724137931No Hit
AAGTTACACGGACCATATGATAAAAAGGCTTGAAAGCGAGAAGACAATAA10.6896551724137931No Hit
GTTCCCATACACCTCAGCAGTCAAAGATGTTGCATTCTGTTATATAACCT10.6896551724137931No Hit
TTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGG10.6896551724137931No Hit
GTTAGGGGACATGGTTTGGTTCTGCAAAGAACTGACTGCAGAGGTCTTGG10.6896551724137931No Hit
ATTCTTCTTTCTAGCGTTCTATGAAGAAATCCCGTTTCCAACGAAGGCCC10.6896551724137931No Hit
GATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATG10.6896551724137931No Hit
GCTGCACCTTGCGCACCACCGGCAGCAGCGGCGCGGCCGCGACGATCACA10.6896551724137931No Hit
GCTTTTGTTGCTCTGCGTTCTTCCTCTGACATTCTTCCTGTCAGTCCAGA10.6896551724137931No Hit
GCAGACATTCTTCCAGATCTTTTCCTAAGCATTGACATAGGACATGTGTA10.6896551724137931No Hit
CGAGTGGAATGGAATGGAGTGGAATGGCTTGAAATGGATAAGAAATGAAA10.6896551724137931No Hit
CGACAGCCCTGTGTCCTCACGGCGCGCAGAGGCGGCGCCGCGAACCTTGT10.6896551724137931No Hit
GAAGGACACAGAGTGGTCCAAATATCCACTTGTAGATCCTGCAAAAAGAG10.6896551724137931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTAGGGATGACACCCCGGGAGACGAGGGCGCATCTGACTCTGAAGGTC10.6896551724137931No Hit
GTTCATTATTAGACTATTTGGAGTTCTGGGAATGAAGAGAGGGTTTCAAG10.6896551724137931No Hit
ACCAAAAGCTGGTTCTTTGACAAGATAAATAAAATTGATACACCATCAGC10.6896551724137931No Hit
GTTCTGCTCTGATTTTAGTTATTTCTTGCCTTCTGCTAGCTTTTGAGTGT10.6896551724137931No Hit
GGCATCAGAGGCTCTTGAGCTGGTGTTTGTTTAGGGCATTGCCTCTGTCC10.6896551724137931No Hit
CTCAGGTGCAGGAAAAAAAGAATTGGATGAGTACATCTCTAAGATCTCTT10.6896551724137931No Hit
ATAAAGGGGATATCACCACCGATCCCACAGAAATACAAACTACCACCAGA10.6896551724137931No Hit
GGTGGTGGGTGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGTGCGGA10.6896551724137931No Hit
ATCGAATGGACTCGAATGGAATCATCGAATGGAATGGAATGGAACAGTCA10.6896551724137931No Hit
ATAAAATATGCACGGCAAAGCAGGCATCACCCTAAGGAGCTTCTTCTGCA10.6896551724137931No Hit
GTGAACGAAATGCTTTAATAAGAACTGGGGGTTGAAGATGAGGCCCCTGG10.6896551724137931No Hit
CCGCAATGGAAGGGCGCCGGCAGCTCAGGCTGCCGGCTGGGTGGCGAGCG10.6896551724137931No Hit
CACACACACGGGAGAAGGGGGAAGGAAAAAGAGGGAGAGTGTATTAATCT10.6896551724137931No Hit
CTCCTTTATCAGTAATTACTAAGTCAAAGAGGCAAAAGGTCAGTAAGAAT10.6896551724137931No Hit
TAGTAGGACAAGGTAAGTCAATACATGTATTTGGCAATTCTGTCAAGATA10.6896551724137931No Hit
TTTCCAAACTGCTCTATGAAAAGAAAGGTTAAACTCTGTGAGTTGAAGGC10.6896551724137931No Hit
CAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCA10.6896551724137931No Hit
TTGCTGTACCAGTCAGTGAGGAAAAAAACACAGAAGTAGAAAGGGCACAG10.6896551724137931No Hit
ATAGAAAATTATTTCAAATCTGGGATTCTACAACCAGTCAAAGCATTTCA10.6896551724137931No Hit
ATACAGATTTTATAAACCATCTCCTTTTGAGGTGGATTTAGGAGCCTGAG10.6896551724137931No Hit
GAGCAAGACTGTCTCAAAAAAAAAAAAAGTGGGCAAAGGACATGAACAGG10.6896551724137931No Hit
ATTTTCCATTGATAATGGCAGTAGAGTTGAATACATGGGTCAGACTATGA10.6896551724137931No Hit
CCCTTACACTCCATTATATTCCAATCCATTCTGGTTGTCTCCATTCCATT10.6896551724137931No Hit
CCCCCTACTGGGCCATTGAGAACTGCTAGTCTCTTCAGGTCCCCTTCCAT10.6896551724137931No Hit
AGGTACTGAGGTTTGGGACCTCAACATATAAATTTGTTGGGGGGTGGGGG10.6896551724137931No Hit
AAGTATAATAATAATAAATAAATAAATAAATAAAAGAATGTTGAATATTG10.6896551724137931No Hit
GGAAGTAGCTGCGTTGGGCGCGATAAAAGAGCCCCTGAATAAAGGCCATC10.6896551724137931No Hit
TACATATGCAGATTGATTGGGATTAAACAATTGCAGCTCCTGGTTAGATT10.6896551724137931No Hit
ACAAGAGCAAAACTCTGTCTCAAAAAAAAAAAAAAAAAATCTATCACTGT10.6896551724137931No Hit
ACATATTGAGTTCTAGTACAGATGAGTGGGCTCTGAATTCAAAGGAGAAA10.6896551724137931No Hit
GTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTTTTTACAA10.6896551724137931No Hit
GGAAAACACCATTTCCATCTATTTCTCTTGGCCCCCAGCAACTCTGTGCT10.6896551724137931No Hit
CAGCTAAGTAAGTAGTTGTTGACTTAAGCCATGAAGATTTGGGGTCGTTT10.6896551724137931No Hit
ATTCCATTCTATTCCGTATCATTCCATTCCATTCCATTCCATTCCATTCC10.6896551724137931No Hit
TATTCTAAAATTGACCACATAATCGGAAGTAAAACACTTTTCAACAAATG10.6896551724137931No Hit
ATCCTCCCGCCTTGCCCTCCCAAAGCACTGGGATTACAGAAGTGAAAGTA10.6896551724137931No Hit
ATCTATTGCTCTGTAACAAACTGAGCAGCTTAAAATAACAAACATTATCT10.6896551724137931No Hit
ATCATATCATCTGCAAATAAGAACAATTATGACTTTTTCCTTTCCAAATT10.6896551724137931No Hit
GCTTTTCTAGTTCTTTTAATTGTGATGTTAGGGTGTCAATTTTGGATCTT10.6896551724137931No Hit
TCACTAAGCTGCCGCTAAACTTCATCTCTGATATTTTTAGCTGTATAACC10.6896551724137931No Hit
ACCCAGGAGGCGGAGCTTGCAGTGAGCCAAGATCATGCTACTGCACTCCA10.6896551724137931No Hit
GTGCTGGCGGTGCCCGAGGACCAGGTGCTGAAATGCCGCCGCGCCGGCCT10.6896551724137931No Hit
CCATTTCTTTTTGTCCACACACTGCAACTTAATGGTTTCTCTGATCTTAT10.6896551724137931No Hit
CCAGGAAGAAGGGGAGCCCAGCCGTGGCTAAGAAGCACATCCGTTACACC10.6896551724137931No Hit
TCTCACAACTGCATTGATGCTGTCAGTGTGGTAACAGGATCTCAGACTGG10.6896551724137931No Hit
CTCCCAACATATACGTAATTGTTACAGAAGGATTTTTTTAGGATACACGG10.6896551724137931No Hit
CATCAAATGCGAATGGAACATTCAGGAAGAGAGAAAGGATTAAAGAAATG10.6896551724137931No Hit
GTTCTTTTTATTGTCCAGCCCCTGACAACCCGTAACCTACCTTTTATCTC10.6896551724137931No Hit
CTAATAAGGCTTGGAGGATACCAAATGAACTTTTCCTTAGAAAGGTTATA10.6896551724137931No Hit
GATCATTACAGAAGATTTTTATGAGGAAGAGAGGAGGAGAGGCAAAGGAG10.6896551724137931No Hit
TCTGCACCTTTCAAGCTCCCCTTCCCTCCCTAGGATACCTTCCTGCTGCT10.6896551724137931No Hit
TTATATCAGTGTTACACTAAATATGTAATAATGCATTACAAATAGGAAGG10.6896551724137931No Hit
GGCACCCCTTGGAAGAATTTGGGGAAGCTGACCAGAGACCTGCATGCTTG10.6896551724137931No Hit
CTCATGTTAAAAACTCTCAATAAACTAGGTATTGAAGGAACATACCTCAA10.6896551724137931No Hit
GTTAGAAGCCCATTCTCCAGCAATGGCTTAAGTATTCTTTCAAGATTCTG10.6896551724137931No Hit
AAGGCATGTGGATATGGAGAGATGTGTGGACAATAAGCAGAACAAGGGGT10.6896551724137931No Hit
TCGCGGTTTTTTGCGGTCAAACCCACCGTTGTTGCGGGGTCGGCGGGGCC10.6896551724137931No Hit
TTCTACTATGAGTAGTTAATATTCTTCCCAGGTGCTGAAGAACCAGAAAG10.6896551724137931No Hit
TTGCCATATTGTTTGATGCAGATGTAACTTTCCTACTGAAGTTAATAAAA10.6896551724137931No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA10.6896551724137931No Hit
GCCGCGATGCGGACACGGCCTGCGCGCTGCTGGAGGAGCACATCCTCAGC10.6896551724137931No Hit
CTGCAAGAGTATATTTGCCTAGCCTTGAGGATTTCGTTGGAAACGGGATT10.6896551724137931No Hit
GTGATGCATGCATGGGGTCTTCTCTAAAATATAGCCTGCAAAAATTGTTT10.6896551724137931No Hit
GGGCTGAGCCGATCGACCCGCAGTCGCTGTCGATCATCCGAAATCATTCG10.6896551724137931No Hit
TCTCTTCTGGTATAATGTGTCTACACTGGCGATCACTGGATTGGGCCTCC10.6896551724137931No Hit
CAACGCAGAGTGCAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA10.6896551724137931No Hit
CACCTGTAATCCTAGAACTTTGGGAGGCCAAGGTGGGAGGATTGCTTGAG10.6896551724137931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)