FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGAGTCTCAGGGTGGTGGAAATGTGTTAGCAGCTTCTATCGCTCAT33.614457831325301No Hit
GCTTCCGCGTGCGCGAGGAGCTCCCGACCGACGTCAAGGGTCTGGCCCGC33.614457831325301No Hit
GCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCA22.4096385542168677No Hit
ACCTGGAATGATAACAATGAGTTTGAAGTATGGGATGGGGAAGCGCGCTG22.4096385542168677No Hit
CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT22.4096385542168677No Hit
GTTCGTAAAGCCTGCAAGTGCTTTTTTGGACTTCATTGAGGCCTTCGTTG22.4096385542168677No Hit
GTAGTCTTGCAGCCCGGTGATGAAAGGGCTGGGAGTTTATGAGACTACAT22.4096385542168677No Hit
CAACTTTACATTATAATTAGAACCTAAGCCATTCTGGGGCTAGAGATTTC22.4096385542168677No Hit
TGCCGAGATAGTCGCTCAGCGTTCCCGAGAAGACCTTGACGATCAACGCC22.4096385542168677No Hit
CGGCATGCCCACCCTACGGGACACGCCGCGTGCGAGCGCGAATGCCGCAG22.4096385542168677No Hit
GCAACTACATTAACACGTCTGCAAAATAACCAGCCAGCATTATGATGACA22.4096385542168677No Hit
CCATTCAACATTTCTCTAGGGAGTTCCAAACTTTTCCACATTTTCCTGTC22.4096385542168677No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCTTTTCGTTCC22.4096385542168677No Hit
TGATTCCATTGGATTCCATTTGATGATTCCATTCGATTCCATTCGATGAT22.4096385542168677No Hit
TAACAACACAATGCGTGCTTTCCGGAAATTACGATGCGGCTTTTAGGACC22.4096385542168677No Hit
GGGGATGATTCCTGCCTTGTAGGATGCTTGTGAAACATAATTGGGAAAGC11.2048192771084338No Hit
CACCGTGCGCACGGGACGGGCGCCCTCGGCGGCGCCGCCCTCGCGGGGGG11.2048192771084338No Hit
CCTCTCTCACTCCTGCCCCTACTATCTATCATGGAATAACATCCAAGAAA11.2048192771084338No Hit
CTCGACAGACCGGTGGCCACCACGGCCGTGGGCGACCAGTCCCACAGCAG11.2048192771084338No Hit
GCTATAAAGATGCCTGGGTTTCTATGAAGCCACCTGGCTATTCCCCTCCT11.2048192771084338No Hit
GTGGTAGGCCGCGCCCGTGCCGGTGGTGCCTCCAGCTTCCTCGCTGACCG11.2048192771084338No Hit
CATGCGGACCAGCGGATCGCCAAGCAGCTCAACCAGCGGTACGCCGAGCT11.2048192771084338No Hit
ATTGGTATTGAATGGAATTGTCATCAAATGGAATTCAAAGGAATCATCAT11.2048192771084338No Hit
AGGATGTAGAGGCTCACCGTCATCTGCATCTGCCCGATGCCCGCGCCCAG11.2048192771084338No Hit
AAACTGGAAACTCTAAAAAGCAGAACACCTCTCCTCCTCTAAAGGAACAC11.2048192771084338No Hit
CTCAACTACTTCATTAGATATTTAACTGAGTTAGAAAATGAAACATTNGA11.2048192771084338No Hit
CTTGCGGACCAGCGGATCGCCAAGCAGCTCATCCAGCGGTACGCCGAGCT11.2048192771084338No Hit
GACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAG11.2048192771084338No Hit
GGCTATTGTTCCTTTTTAGCTGGATTGATACTAAGCGTCTTCTTATCAGT11.2048192771084338No Hit
GTAGTCTTGCAGCCCGGTGATGAAAGGGCTGGTAGTTTATGAGACTACAT11.2048192771084338No Hit
CCCATTCTGGTACATTCTCAAATTCCTTATCCCCAGGAAAGAGCACAGGC11.2048192771084338No Hit
CGGTGGCTCATGCCTGTCATCCCCGCACTTTTGGGAGGCCGAGGGCCGGT11.2048192771084338No Hit
GCCTTGCTGCAACGCGGCCTGCGCTTTTCCGTCGGCATGGTGGCGATGCC11.2048192771084338No Hit
TCGTTGAGGGATCGCTATCGCGACCTGCTGACGCATTGGTCCCCATCGTT11.2048192771084338No Hit
ATGGTCGGGCTCGCCCTCGAGCACGCGCACCAGGCAGGTCCCGCAGATGC11.2048192771084338No Hit
GGTTCAACTCTGTTAGTGGAGAACACACATCACAATCAAGGTTCTGAGAA11.2048192771084338No Hit
GGATCAACGCAGAGTACATGGGGAGGAATTGAGGCAGCCAGCGCAGGGGC11.2048192771084338No Hit
GAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATG11.2048192771084338No Hit
AGCCTAAGCAGGTAAGGATTCTAGGACTCAAATTCTGCTGAAATTCTCCC11.2048192771084338No Hit
GTTCTGCTCTGACTCCAGGTTGGGACAGCGTCTTCGCTGCTGCTGGATAG11.2048192771084338No Hit
GGCTTAAGTGATCCTCCCACCTCAGCTTTTGGAGTAGCTGAGATTATAGG11.2048192771084338No Hit
ATCTGGGACAGCTCCTCTCTTGTGGGTGTAGGAAGCTGGAGTCTGGGGGC11.2048192771084338No Hit
GCTCATCGACTACGACGCGGAGATCCGCGCAGCCATGGTCGGCCTGTTCA11.2048192771084338No Hit
GCATTAACAGAGCATTTTGTTTCTAAAGTAGACCTCTTATGTCATCCAGA11.2048192771084338No Hit
TTCCACATGTGGCTCTTTATGTAACAGGCAAAATTAGATTGGTGTGCTTC11.2048192771084338No Hit
TTGTAGGGGTTGTCTACATTTCCTGAATTGAGGTTGGTGAAATTTTTATG11.2048192771084338No Hit
CGATTGTTCAGGGGTCTTGGTCATTTGCTATAAAACTAGTAGCTGCTAGC11.2048192771084338No Hit
GCATTGAACATAATGGGAAAACAAGCACATGGAGACTCTGAAAGGTGAAA11.2048192771084338No Hit
GTGTTCTTTTCAAAGGTGACAGAGGCCCCCTCATGGGATTGTTTCATGAT11.2048192771084338No Hit
ACTCGGGTCAGCCAGTTCAAAGCTGCAAATACCGTCTGCTTCCACCTGCT11.2048192771084338No Hit
GCTATGGCCACTCCTGTAGAGACAACCCAGGCTACCATGTAGGCAGAGAA11.2048192771084338No Hit
ATCTACAACTCTTGAAAAAGAAGTTCCTGTCATCTTCATCCACCCTTTAA11.2048192771084338No Hit
TCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG11.2048192771084338No Hit
GGATTGCAATGGAATGAAATGGAATGGAATGGAATGGAATCAATCATGGA11.2048192771084338No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT11.2048192771084338No Hit
CGATTGTTCAGGGGTATTGGTCATTTGCTATAAAACTAGTAGCTGCTAGC11.2048192771084338No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA11.2048192771084338No Hit
GGTGTGCTGTATTCAGGAGACCCATCTTACATGCAAAGATGCATATAGGC11.2048192771084338No Hit
AGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCATCTC11.2048192771084338No Hit
GCTTTGTTCTTTTTGCTTAGGATTGTCTTGTCAATGCAGGCTCTTTTTTG11.2048192771084338No Hit
GTGTTCTTTTCAAAGGTGACAGAGGCCCCCTCATGGTATTGTTTCATGAT11.2048192771084338No Hit
GTTCGGTACGCTGCGGCAATCGATGAACGCCGAGGCTGACATGAAGGCGC11.2048192771084338No Hit
GTCAGCGCCCGTCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA11.2048192771084338No Hit
CTAGAATACAAGACCCAAATGTATTTTTATTGCTGTCTTCTCAGTGATTG11.2048192771084338No Hit
GCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGA11.2048192771084338No Hit
GAATAACATGGGGAGCATTTAAAACTTGTCAGAAACCTGGAATTCCCTTT11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)