FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGAAGAAGAGGACAAAAGACAAGCTTTTCCAGTTTCTGAAACTGCG52.9940119760479043No Hit
GGATTGGCTTCAGGTAGAGGTCCTCCTTCTTCCCAGGGGTATAGTTGGGC42.3952095808383236No Hit
CGCTCACGCCGTTCCCGTCCGTTCCCCCGGTCAGGCCGCGCTCGGAGGAC31.7964071856287425No Hit
ATCTGTAACGGCGTTGAGCTGCGCTTTCTCCCGAACATGCTGCTCCCTGA31.7964071856287425No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA31.7964071856287425No Hit
CCTTAGAGCAGCAAGGCACGCTTCAAAGCGCGAGTCATGATATTCACTTG21.1976047904191618No Hit
ACTATACTCAAAGTATGGAATTCGGAGAATGGCTAAGAAAAGTAACAGAT21.1976047904191618No Hit
GTAGGAGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATTGAACCTCTG21.1976047904191618No Hit
GTCCTGCATCCTCAAGACGGCCCTGGGGGTGGCAGAGCTCACTGTGACCG21.1976047904191618No Hit
GAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACA21.1976047904191618No Hit
TTATTGACCGCATCCCTTTTCCCCGCCCCGATGATCCACTTATGTCGGCG21.1976047904191618No Hit
CTGGATGTTCTGTGTCCGGAGTGGGTGGTGGCGGCGGACGGACGGTGCTG21.1976047904191618No Hit
GTAGTTATCAACAAGGTTAATAACGATGTCGCGGGCGTCGTCATCGTCGG21.1976047904191618No Hit
ATGTATGGGCGGTGGAAGGCGCGATGAGCGCGCAGGCGCTGCGCACCGCT21.1976047904191618No Hit
GCCATCTAACATGTACCAGGAATGCAAAAAGAACAGTCATCTCCAGCTTC21.1976047904191618No Hit
CTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAACA21.1976047904191618No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT21.1976047904191618No Hit
CACTGGATTTCATTCCATAATTCTATTCGATTCCATTCGATGATGATTCC21.1976047904191618No Hit
GGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTA21.1976047904191618No Hit
ATTCTGATGTGCAGCTGAGTTTGAGATCTAATAAGTTAAAGATACTAAAA21.1976047904191618No Hit
GTTGAGGAGGCATGCCGGGACTGTCCTGTGATGCGGGAGTGCGCGCGGGA21.1976047904191618No Hit
CCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCG21.1976047904191618No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC21.1976047904191618No Hit
ATACGGTGCTGAAGGTTTTCCTGTCGATTGAGGAGATGTGCCCTCTCACG21.1976047904191618No Hit
AGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGT21.1976047904191618No Hit
GATCACTCTTAAACGCCTCCACGAGACGTTCCGCAAGCTGTACTTCCGGC21.1976047904191618No Hit
GCACTGCCTCATCGCATCCTCTACTCTAGGACCCTCTCCCTGGACCCTCA10.5988023952095809No Hit
CGTCCGTGGCTGCTTGCACCGCTGGTGCAAGCAGCCACGGACGCGCACCA10.5988023952095809No Hit
ATCTTGTACCAGGCCCAGTCAGTGACAGGTGGCACGGACTCTGACTTGCA10.5988023952095809No Hit
TTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCACC10.5988023952095809No Hit
AGCCAGAGAGTTAAACAGAGGTTAAGCAAAATGGGTCTCGAAAGCCATGG10.5988023952095809No Hit
GTCCGGGGGGTTGTACTCGCGACAGTTGGCGATGACCCGCTGCAGGTCGG10.5988023952095809No Hit
GTGTCATGGTTGCTGCGATTTTTCGGCGGTTAGCGTGATCTTGTCGAGTG10.5988023952095809No Hit
TCTAGATATCGCTCAAAACACTCAATGACAGACGTATCAAGAAGGGAATC10.5988023952095809No Hit
TTGCAGAATATGGCAAACGATGAAAATAACAGCTGCCACCACCAGCATTG10.5988023952095809No Hit
CATTATGACAACAATGTCGTCTACGAAGATCGAGCCATGTATTAAGAATT10.5988023952095809No Hit
ACCAGGGTTCAAGCGATGAATCTCTCGCACAGTTTCCGCATACAACCATG10.5988023952095809No Hit
GTGCATACATCAGAGGAGGCTCGTAGGAATACTCTCATAAAAGATACCAT10.5988023952095809No Hit
GGCTGGTACTGATCCGCGGGCGGTGGAGTGGTGGGAGTGCTGGCAGAATC10.5988023952095809No Hit
CTCACGAGTTAAAGGACAAAGAAAGAAAAATTGTCAGTTGTGTTTTGGAG10.5988023952095809No Hit
GTTCCAATACCCCGCCGCGTACCACCCATCTTCCGCGGCAACCGATGATG10.5988023952095809No Hit
CATAGGAGCATCTGCTCACTTGGTTAACTTCAAAGGAACAGATACAGTAG10.5988023952095809No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC10.5988023952095809No Hit
GCTCTGTGCTGTTCAGCCCATTGATCACCACTTGAAGACCACCAAAGGAA10.5988023952095809No Hit
GTCTACGCTAAAACGAGTTCGTAAACAACATGCAACACACCTGTGAACAG10.5988023952095809No Hit
TCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCA10.5988023952095809No Hit
ATTCAAGAAAGTTCCATTCCAGTCCATTGCTTTCCAGTCCATTCCATTCC10.5988023952095809No Hit
GTGTTGACAGTAGTGGGGCTGAATGCCTTCCATACGATGGTTTCACAGAT10.5988023952095809No Hit
CCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCA10.5988023952095809No Hit
AAACCCCATCTCTACAAAAAAATACAAAATTAGGCCAGGCGCGGTGGCTC10.5988023952095809No Hit
ACCTGGCACTTCTGCATGTATGTCATGGACCAGAAGCACATACTAAGGCC10.5988023952095809No Hit
GGTTCTCCATGAGTGCCCCACCCCTGCAGCAAACTTCTGCCTGGGCATCC10.5988023952095809No Hit
GTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATA10.5988023952095809No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT10.5988023952095809No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT10.5988023952095809No Hit
GCCGTACGCGGAGTGAATGTCGACGGGGTAATGCGGACACTGCAAGCGCG10.5988023952095809No Hit
GATGACCGCTAATCCTAGTGGGATATCAGACCAGACCATTCCAGCCAAAG10.5988023952095809No Hit
CTTGGCGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGT10.5988023952095809No Hit
CTATAAGGGCACCGAGTACGCGTCTGTTTACCTGCCTGGCAAGGTTGTCG10.5988023952095809No Hit
GTCTAACTGTTCCTGCAGGTTATCGACGATCTGCGACCAATTCTTACGGC10.5988023952095809No Hit
TTATGGCGTTGATTATGCGGATCCGCGTTGCGCCAAACTGGATCTTATCT10.5988023952095809No Hit
GTAAGCAATCTGTGTGCATGTGTACATATGCATCTGTTTTCAGGGAAAGA10.5988023952095809No Hit
ATGCCGACACTGTGAACGCTATCAAGCAGCATTGCCGTGGGGTGGCGTTT10.5988023952095809No Hit
AACACATCCTGAATGGTTTTGTCAAGTGTTAGGTTGCCATATCCATCATT10.5988023952095809No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT10.5988023952095809No Hit
TTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGG10.5988023952095809No Hit
GTTTCACAGTGGCTGCACAAAGGTGTTTCTGCTGACGATACTGCACCCGC10.5988023952095809No Hit
GCCTGGGTAAGTTCGTGAATACGGTAACCACCTAGTTGGTCCGCTCCCCC10.5988023952095809No Hit
GCAGCCTGGTCTGCATGGGCAGCCGACCAAGGGGACTTTGAGAAACCAAG10.5988023952095809No Hit
CTCCCAGTTCTGGAGGCTGGGAAGTCCAAGATCAAGGTGCTGGCAGATTT10.5988023952095809No Hit
ATGCCGACACTGTGAACGCTATCAAGCAGCATTGCCGTGGGGTTGCGTTT10.5988023952095809No Hit
GCTGTTGATCTCTCGCATGGAGAGTGTGTGGAAGCTCCTTAAACCCTGTC10.5988023952095809No Hit
CCCCATGTCCATTGGCGCCATGGTCGGTCCTGAAGCATTCACCGAAGTTC10.5988023952095809No Hit
GGTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT10.5988023952095809No Hit
GTTCAAGTACAGGGGCCTGGTCCGCAAAGGGAAGAAAAGCAAAAGACGAA10.5988023952095809No Hit
AAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTA10.5988023952095809No Hit
CTCGTACGTTGGTTTCTGCACTTATTGGGGATGAGCCGCAGGCCGTCACC10.5988023952095809No Hit
CCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGCGGG10.5988023952095809No Hit
CATTGATACTCCTTTGTGATGCAGTGCGGTGCAGTGTAGTGCAGTGTAGT10.5988023952095809No Hit
TTTTTGGTCTCCATCATCAGGAGATTGCAGGTTTCAGTTTTTAAAATATG10.5988023952095809No Hit
GCTCTGGTCCTTGGCGTGTCACACAGTCCACTTCAATTCTCATTATCACA10.5988023952095809No Hit
AGCTAGGATTACAGGCATGCGCCACCATGCCCAGCTAATTTTGTATTTTT10.5988023952095809No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG10.5988023952095809No Hit
ATAGCGATGTCTTGGCTACTCAAGCAACGAGTATTGACATCACCGCGGAG10.5988023952095809No Hit
GCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGA10.5988023952095809No Hit
AGCCAAATTTCTGGACTCTACAGGCTCAAGATTAGATTATCGTCGCTATG10.5988023952095809No Hit
GCAACCCCCACCTCCCGGGTTCTATCAATTCTCCTGCCTCAGCCTTCCGA10.5988023952095809No Hit
GGAGATCTCCTGCTCCTGTTGTTCAATAGCGGTGGTATCAAAACCTTGTT10.5988023952095809No Hit
GGTTCGGGTTGGTAAATCCGAATGTGACGGTGGTGTCTTCAATGACGGAG10.5988023952095809No Hit
TCCGTAGGCTTCTCAACAATCTTTCTGAGGCCACCAAGACTCAAGATGAG10.5988023952095809No Hit
CCTCCGAGGGGCAGGCGGGCGGGGAGGCCATGGCGAGGGTGGCTGCTTCC10.5988023952095809No Hit
CCGGTGGGCTGAGCGCACGCATCGGTGCCCTCGTATGTGGCCGAATTGGT10.5988023952095809No Hit
CTGCAACCTCCAATTCCTGGGCTCAAGCGATCCTCTTACCTCAGCCTCCT10.5988023952095809No Hit
ACATTCAACAAGCCTCTCTTGGGGTCTGGATCGAGACTTCTTTCCTGTAA10.5988023952095809No Hit
CCTATAGTCCCAGCTAATCGGGAGGCTGAGGCAGAAGAATCGCTTGAACC10.5988023952095809No Hit
GCTCTCAGATGATGCTGATGCTGCCAGTCCGTAAACCAGACTTTTTATAG10.5988023952095809No Hit
GTCTTTGGTGGTGTGAGAACCCTATCTACTGGTAGATAACTTACTTATAA10.5988023952095809No Hit
GTCTTCAATAATGTCAACGTTTTCACAGCCACCGTAGTAGCGACGGCCCG10.5988023952095809No Hit
CCATGGACACTTCATTCGTCCACTCAGACTCATACTTCCACTTATTCATC10.5988023952095809No Hit
CGGCGCGCTGCGGCAGCTCGCAGCCGGCGGCGAGCTCGACGCGATGCTGC10.5988023952095809No Hit
CCTTCTTCGCTTTCAACGCCGGCCACATCGCCGGCTCTGTCAACGTGCGC10.5988023952095809No Hit
AGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10.5988023952095809No Hit
ATTCTGGATTACCTGAATATGGCAGATAAAAATCGCCAAATGATTCTCGG10.5988023952095809No Hit
GTCATACTCCGCACTATGTGGCTATTGGCGATTCCTACACAACAGCCTCT10.5988023952095809No Hit
GGGGTTTCCCGCCTCGCACCCCCACCTCTGGACTTGCCTTTCCTTCTCTT10.5988023952095809No Hit
ATTCAGCTCCCTGAGCTGAGCCTTGAGGTCCGAGTTCATCTCCAGCTCCA10.5988023952095809No Hit
CCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCC10.5988023952095809No Hit
CACCAGTACCACCTGTGAATGAGAGTCAGCTCGCCTCTCACCTCCCCCGT10.5988023952095809No Hit
ATTCGAGTCCATTCGATGATTCTATTCGATTGCATTCGATAATTCCATTC10.5988023952095809No Hit
CCAAGGACACTTCATTCGACCACACAGACTCATACTTCCACTTATTCATC10.5988023952095809No Hit
CCCCCATCTTCAGCTTCGCACCACTGTGGCTAGCCGACTACAACAACCAT10.5988023952095809No Hit
GGTCATAACCTAGAGGCTCTGTGCTAGACCAATGATTCCCCTCCTGCGTA10.5988023952095809No Hit
GAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCTAAACTACCAA10.5988023952095809No Hit
ACCATGTGGCCTCAGTCCTGTGCCAACGGGCCAAGGTGGCGATGAGCCAC10.5988023952095809No Hit
GAGTGGCTGCTCCCCATTGGAATCTGTGAGGATCATTTCCTCTCCAAAGT10.5988023952095809No Hit
CGATGAAGATGATGATAAGTAGGAGCAGCCATCCGATAGTGGCACGGCTA10.5988023952095809No Hit
TCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGA10.5988023952095809No Hit
TTTTTTTTTTTTTTCATTGATTTCTGCTTTACTCTTTATTATTTCCTTTT10.5988023952095809No Hit
ACAATGATTATTAAAGATTCTGACCGGGAGACCTGCCCGGATCGGTGGTT10.5988023952095809No Hit
GTACTGTCGCACCGGCTCAACAATCTGAGTCCACATCTGTGGGCGCGCCT10.5988023952095809No Hit
GGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA10.5988023952095809No Hit
TGTCACGACTGAGAACACTGCTCGTGACTTTGATATGGCTCGTCAATATC10.5988023952095809No Hit
GTGTAGCGGCCGGAGGCGTCGGTTGCGAGGGCGATGCCGCGTCCGGTGTT10.5988023952095809No Hit
ATTCAACCCACAGTAGTCCCTTTACCCTGCATTTTTCCCACCAGGCAAAA10.5988023952095809No Hit
ACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTA10.5988023952095809No Hit
CCTCTGCAGGAGCCTCATGACCAAGCCCGGCTGCTCAGGCTACTGCCTGT10.5988023952095809No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT10.5988023952095809No Hit
TCTCCCATGGTTAACTGCTCAGACGGCCACACAAACAGCCCTGTGGGATA10.5988023952095809No Hit
GGGTGGCTGTGCATGGGTCGCCCCCATGGATTGCGACATGACCCTGGCAG10.5988023952095809No Hit
CGCCTATATGGGGGTGGAGTGATGGCGATGAGTGAAAACACTGGCCTTTT10.5988023952095809No Hit
CTTCACCAGTTTGGTGATCAGCCCGCCGACCTTACCAATGGGAAGCTTGT10.5988023952095809No Hit
TCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT10.5988023952095809No Hit
GGGGCGGCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAA10.5988023952095809No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)