FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length146
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGTGGTGGATGTATTGGTGTTTCTACAATTCTTCCTTTTTTTTTCCTT44.25531914893617No Hit
GAACTGCAGCTGCATGGGCTGACTCTTATGTCCCTCTAACTCCCTGGCCA33.1914893617021276No Hit
GTTACCAGTATGTAAATATTCACAAGGTCATAGAATGGATGAGTGAGTTG22.127659574468085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22.127659574468085No Hit
CAGTTATCTGGCCATTGGGTAATGTTCCAAAAATGATCATAGCTGCCTCC22.127659574468085No Hit
AGGTAGGAAACGTTTGGCAGGAGAAGAAACCAGAAGCTGGAGCTAGTAGC22.127659574468085No Hit
GGTTAATGCAGGTTTTAGATTTTACCTGTGGAGTTCCTGAAGGTGAAGTC22.127659574468085No Hit
CCCCCATTAGAATGCCTGGATTTGCCCAGGTTTATCTATCATTAGAATGC22.127659574468085No Hit
AGGCAGTAGTGTTTGTGTAAATTCTTAAAGGATTTTAGAGAATAGGAGGA22.127659574468085No Hit
ATTTTGTATACTACTTGCTCCAATGGTTATTGTTTGTCACAGGCAGCTGC22.127659574468085No Hit
GTCGCACACCCTGCGACGATGACCCATGCAGATATGGGTGATGAGGCCCG22.127659574468085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC22.127659574468085No Hit
GGGAACTCCTATGTCATAGGGAACTCCCTGTGAGGCGATCAGTGCAGGCT11.0638297872340425No Hit
GAATGGAATCGAATGGAATCATCGAATGGACTCGAATGGAATAATCATTG11.0638297872340425No Hit
GAGCACTGGGTGGTGAGTTCACATAGGTTTGCTTGCTCTCCCCCTCAAAC11.0638297872340425No Hit
GTATTTCTTTTAACGTGCATTTGGAGTTTGAGCACTTCCTGTTTAAATTT11.0638297872340425No Hit
TGCTAGCGGTCTATCACTTTTGTTGATCTTTTCAAAAAACCAGCTCCTGG11.0638297872340425No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG11.0638297872340425No Hit
CTATACCTTTTTTCTCACTTGTATTTTTCATTTCTTTCTCTCTTCTCTAC11.0638297872340425No Hit
CCTGAGATGTTCTGGGGTTCTGGGGTTGATTCAAGCACACATGAGCTTGT11.0638297872340425No Hit
GTCTACAAAAGATATGTGTGATTCTTTTATCAACAAAACATATGTGTTAA11.0638297872340425No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGA11.0638297872340425No Hit
CAACCAGATTTGTGGTGCTGAGCTGGTAACTGGTTTCATCCAACCAGGTA11.0638297872340425No Hit
GGCGGTCAGGCGCGAGAGCTCGACCTCGGCACCGCGGGCCCACAGGCGCG11.0638297872340425No Hit
TTCCGGTAGGGTCCGTTCGATGTTGATTCCATTGGAGTGCATTCGATGAT11.0638297872340425No Hit
ACGGAATCCAATGTAATCATCACTGAATTGAACCCAATGGAATCATTAAA11.0638297872340425No Hit
TAATAAAGCTAAGGAAAAAGAAATTTAATTTATTTTAGACAACGGATTAA11.0638297872340425No Hit
GTCTATTAGGTGTGTACGTAGAAAACGCATTTATGTGAAGTAAATTATTA11.0638297872340425No Hit
TTGTTAAGCTTACAATAATCCACTCTCATTCTCTCTCCAGGATCCATTTG11.0638297872340425No Hit
GGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTC11.0638297872340425No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA11.0638297872340425No Hit
ATTCTATTGCACTCGGGTTGATTGATTCCATTCCATTGCATTCCATTCCA11.0638297872340425No Hit
ATATGAGCTTTTTCTTTAAGCTTTGCCACTGGATTCCTGTATCTCTCATT11.0638297872340425No Hit
GAGTCCCTCCTGTGTGCACCACTGTACTCAAGTCTGGGTGACAGAGCAAG11.0638297872340425No Hit
GGGTAAGCCTTAAGCAAAAAAAGTGACCTTTTCCTTCATCACTCTTTCAT11.0638297872340425No Hit
ATGGAATCAAATGGAATCCTCAATGAATGGAATCGAATGGAATCATCATC11.0638297872340425No Hit
ATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAGGAAGGGGGGTTACA11.0638297872340425No Hit
AAATGTGAGAGATAAATACTTTCTCAAACAAAAATTGAGAAATTTTGGTG11.0638297872340425No Hit
GGGATTACAGGCACCTGCCACCATACCCGGCTAATTTTTGTATTTTTAGT11.0638297872340425No Hit
ATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGC11.0638297872340425No Hit
AATCTCAGGACACTGGAATCTTCATGTTTAATAAAGCCTTTATGGCAAAA11.0638297872340425No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAACTGTC11.0638297872340425Clontech Universal Primer Mix Long (95% over 21bp)
GGTTCAGAAAATCTTGATTTATCTGGCTTAGAATTAATATTTTGTGCCTC11.0638297872340425No Hit
GAAACTTACTCGTGATATGTGTCCTCAACTAAAGGAGTAGAACCTTTCTA11.0638297872340425No Hit
GGTCTCAGTACTCATCTTATTTGATACATCAGCAGCACTAGCAATGGCTT11.0638297872340425No Hit
TTCCATGTAGTTGTGCGGTTTTGAGTGAGTTTCTTAATCTTGAGTTCTAA11.0638297872340425No Hit
AAACTAGACAGAAGCATTCTCTGAAACTTCTTTGTGATGTGTGAATTCAA11.0638297872340425No Hit
GCCCATCTAATTTTTGTATTTTCATAGAGACAGGGTTTTGCCATGTTGGC11.0638297872340425No Hit
GTCCTTGTCCAGGTATTCCAGGGCCTGGTCGAGGCTGTGGCAGACGGTCG11.0638297872340425No Hit
CATCACGGCCGACTGCTTGCCCGCTTCGGGGTACTTCGCCACCTCGCGCG11.0638297872340425No Hit
ATCTTATGTATAGAAAACCTTTAGGATTTCACCAAAAAGCTGTTAGAACC11.0638297872340425No Hit
GCCTCGGCATCATCGATCGCGGCCGGATACGGGGCGTCCGGGGCCATGCG11.0638297872340425No Hit
GCCCGTGGATATCCCGTGTATAGCTTGTGAGGATCTTGTGGGAAGCGTGT11.0638297872340425No Hit
GCTTAGGGACTCTTTTTTGTGATCTACTGCTCAGACAGATCCAAAGAGAG11.0638297872340425No Hit
GAGGGAATTTGTTACCACCAGACCTGCCTTGCAAGAGCTCCTCAAGGAAG11.0638297872340425No Hit
GCTAACAACAGATTGGAATAATAAACCCTTCTGTTCTCGGAAACCAGAAA11.0638297872340425No Hit
TCACAAATAAGTTTCTGAGAATGCTTCTGTCTAGTTTTCATGGGAAGATA11.0638297872340425No Hit
GGTGGGGGTCATCTCTGGATTCAAGCTGGAGTGCAGGAAGACCACCTGCA11.0638297872340425No Hit
GTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCCTGTCTCTTA11.0638297872340425No Hit
CCCCATATCCAATCTGTCTGGAAATTCATCTAGTTCCAACTTCAATATAT11.0638297872340425No Hit
CTCTTGGATCTGCCTATGGGGGCATTGTGACAAATCTCTGCACTGATAAC11.0638297872340425No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG11.0638297872340425No Hit
CATGTAAAAAGCAGACAGCAGCATTCTCAGAAACTTCTTTGTGATGTTTG11.0638297872340425No Hit
CTGCTACATCAGTGAGTGCAACTAATCTGATAAGCAGAGGTCCATGGGTG11.0638297872340425No Hit
GTAACTAGCTTGCTTTTGCTTTTACAGGCTCACAGGCAGAAGGGACTTGC11.0638297872340425No Hit
AATGTATCCTTTTGATGAAAGAGTAGAAAGATGAAGAGAAAGTTTAAAAC11.0638297872340425No Hit
GTTTTTGCTTTGCTTTCTTCAGTCTTCATTTTCTCCAAGAGCTTTGCTAC11.0638297872340425No Hit
GCGCCACGAGATCTACACTCTTTCCCTACACGACGCTCTCGTTTTTCAGG11.0638297872340425Illumina Single End PCR Primer 1 (96% over 33bp)
AAGGGATGGGTCGTCAAACTCCAAATGGTCATAAAAATGGAGACTCGGAC11.0638297872340425No Hit
GGAACGAGGACATCCACGAGCAGGACCGGCAGTCCAGAGATGCGATGGCG11.0638297872340425No Hit
GGCATAGCTGTCTCCTTTGACTTGTTCGAAATCGGTTTTACCGGAGACTT11.0638297872340425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCGC11.0638297872340425No Hit
GCTGGTCTTAGGGAGGCCATGGTGATTGTGATAAATAAGACAAGCCATTC11.0638297872340425No Hit
ATTCCTAAGTATTTTGTGTGTGTGGTTATCGTAGATGGGATTGAGTTCTT11.0638297872340425No Hit
GATAGAAAAGCCAGTACCACACGTGTTAAGAGGCAAGTTCCTGTGTTAGA11.0638297872340425No Hit
CGCCGGTGATGTCCTCAAGGGCGATCATGGCGCCTGGTTTCGCGCGATGT11.0638297872340425No Hit
CACACACACACACACACACACACACACACCCGGCACTTATACACATATCC11.0638297872340425No Hit
GTCATAAGAGAGGGAAGCACACAGGACTGCAAACTAACACCCAGTAGCCA11.0638297872340425No Hit
GGTCAAGGTGGACCACGGTGGGGTTTGACTCAACATTCTGGATTCCCCAT11.0638297872340425No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)