FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGACAAAAGGAAACACGATAACAGAAACAGCTTAGTGGCATTATGCTA33.1914893617021276No Hit
GTGTTCAAACAGTGTGTGTGTGTGTGTGTGCGTGTTACTTTTTATTACAA33.1914893617021276No Hit
GCACTGACCACCATCGGCGCACCAGATTCTGGCGGAACGCCAGATGGAAT22.127659574468085No Hit
ATCCTCAAAGTGTTGAAATAGTGTGCTTTGATATGCCTGGTGGCTCCCTG22.127659574468085No Hit
ACCGACCAGAGGACCTGGCCAGGCTCAGTGAGAGCCCAGCAGAGATGAGG22.127659574468085No Hit
AGCTGGGATTACAGGCATCCATCACCACACCCAGCTAATTTTTGCATTTT22.127659574468085No Hit
CTCATATATAACTGGATTAGGGAGAGGATATGTGCTGAGCTGTTCTCTCT22.127659574468085No Hit
ATCTAATCCGAGGTCCAACTCAAATGTGTCATGACCAATAACTTATATTT22.127659574468085No Hit
TTCTAATGGGAGAGGCTCTTGGCCGACCTCCAGTCTTCCAATAACAGAGA22.127659574468085No Hit
GTTCAACAGGAATGCTGTGGGAGATTTCCCTTGTGATAAAACACAGTGCC22.127659574468085No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.127659574468085No Hit
GCCAGCTAACTCAGCCAGCAGTTGCATGTTGAAAAAGCTCCCAACTGAAA22.127659574468085No Hit
ATGTAATGGAATGGAATGGAACGGAATGGAATGGAATGGAAAGGAATGGA11.0638297872340425No Hit
GCCAATGGGGCGAAGCTACCATCTGTAGGGTAAAACTAACCTGTCTCACG11.0638297872340425No Hit
GATTCCATTCGAGGATGATTCCATTCGATTCCATTCGATGATTCCATTCA11.0638297872340425No Hit
CACATAGTCTTTTCATTTGTTTGTCTTTAATTTATTTCAGCAATGTTTTC11.0638297872340425No Hit
TCTTTACTGTCTCCACGGTTTGGCCCTTTCTAGAACATCACAGAGTGGAA11.0638297872340425No Hit
TTGATGAAGTGATCGATCTGCGGGAGGCTCATAAGGTTTCCTTTGGTGTT11.0638297872340425No Hit
GGCCAGGCCTATGCCGCCGTCGAGCATCCGAAGGGCGAGTTCGGCATCTA11.0638297872340425No Hit
CCTGATGCCCGCGGTAGCCCGCCAGGTCCGTCGCCGCCAGAAGCCCAGCG11.0638297872340425No Hit
NTCCAGTTTCCTTTGTCCCAAAATGTGCTCTGGTGGACTACAATCCCCCT11.0638297872340425No Hit
GCCGCGCGCCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGG11.0638297872340425No Hit
ATTTATAGCACTAAATGCCCACAAGAGAAAGCAGGAAAGATCTAAAATTG11.0638297872340425No Hit
ATTATAATTAGGAGAACAGATTTCTCTTCCTTCCTTGGGCTTAATCCAAG11.0638297872340425No Hit
GCTTAGGCAAGTGTTACATCACCTCTGTCATCAGTTCAGGGATACGTGAA11.0638297872340425No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC11.0638297872340425No Hit
GTGTACATGTCCCACATTTTTTTTATCCAGATTGCCATTCTGTGTCATTT11.0638297872340425No Hit
GTATTATGGCCTCTGTGGTCCTTACCACCTTGCCTGGCACCACCATCCTT11.0638297872340425No Hit
GATTCCATTCGAGACCATTCGTTGATTGCATTCAATTCATTCGATGATGA11.0638297872340425No Hit
GCAGTGTGGCTTGCAAACAGAATGCTGTGCAATACTTGGAGTTCGTATCC11.0638297872340425No Hit
GTTTTGAAGGAGCAGTTTTGAAACACTCTTTTTGCGGAATCTGCAAGTGG11.0638297872340425No Hit
CCCTAACACAGTGGTGTGAATATAGCAAAGATTAAAATTTTTTTAAAGTA11.0638297872340425No Hit
GCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCTGTCTCTTA11.0638297872340425Clontech Universal Primer Mix Long (95% over 21bp)
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGAGCGTCGTGTAGGG11.0638297872340425No Hit
GGCTTAACAGACCCAAGAAACCGAAAGCCAAGCCATCAGTAGAGGGAGTT11.0638297872340425No Hit
ACTGCAGCCTGGCAGTGATGTCGTTGCACTCAGGCTCCTCACATGTGTCA11.0638297872340425No Hit
CCTTTCCAACAGTCCCTCAAAGTCTTAACTCATTTCAGCATTAACCCAAA11.0638297872340425No Hit
CCTCAGAGCACTCCAAATATCCACTTTCAGATTCTAGAAAAAGAGTGATT11.0638297872340425No Hit
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC11.0638297872340425Clontech Universal Primer Mix Long (95% over 21bp)
GGCGGACCACCGAGATCTACACTTTTTTTTTCGTCGGCGAAAAAAAAAAA11.0638297872340425No Hit
GATCAGCATCCGGCATCGGCGGAGGCGTGGCGCGCTTTGGTCAAGACCTA11.0638297872340425No Hit
CCTGGGTTTGGGTCAGACATTCTCAGAAGAGTATTATTTACTTTTAAGTA11.0638297872340425No Hit
ATTGGAAGTAAAGCACTCCTCAGCAAATGCAAAATAATGGAAATCATAAC11.0638297872340425No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTGGAGAATACACATAATA11.0638297872340425No Hit
CTATTGCATGGCAACAATTGAGAACACAAATTCATCTGCCTCAGCTAATA11.0638297872340425No Hit
CATAATAATTTTCTTTGGGTGTCATGACATGGAATAATTGGGAAGAAGTC11.0638297872340425No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT11.0638297872340425No Hit
AGTAAGCACTGGTAAGAACCAAAAATTTCAAACAGTCTCAGGAGTTCATT11.0638297872340425No Hit
GACCCACAATGTAACTTTCTAGTGAGAGAGTCAACATTTGATAGCTGAAA11.0638297872340425No Hit
TGGCCAGGTGGTACTGCAGAGGAATGTAAGTCATTTCTTTCTTAGCATCT11.0638297872340425No Hit
ACCTGGTCCTTTCCCTTTCATTTCATCCCAGAACCCACCATCAACTCAAG11.0638297872340425No Hit
CATACAATTAGCCATTTTAAAGTATGCAACTCAATGACGCTTAGTGCATT11.0638297872340425No Hit
AGTGAAGCTTGTTTGTGGTGAGCTTTGGTGATCAGTGCTCCAGCGGCCAA11.0638297872340425No Hit
CTTCTCGACGCTCACCGGCCAGCCGCTCGAAGACGCCGACGCCACCAGCC11.0638297872340425No Hit
GTCCCCACCCAAATCTCAAAATCTCATCTTGAATTGTAGCTCTCATAATT11.0638297872340425No Hit
GGAATATATAGAGATAGATTGTAGGACCAAAGGGCGTACATAGGAAGGCC11.0638297872340425No Hit
CTCTGAGTCACCAGAAAGCCACCTGCAATGTTGACAGAGGATATGAATGC11.0638297872340425No Hit
GTGTTCAAACAGTGTGTGTGTGTGTGTGTGCGTGTTACTTTTTATAACAA11.0638297872340425No Hit
CCAATCCGAAGGGCCGTCGCGTCGAAGCGCGCTTCCCCGATCCGCTGATG11.0638297872340425No Hit
TTATCACCATCAGCAATTTGATGTCTCCTTTCAGGTATTTTTCTCTGCAC11.0638297872340425No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCA11.0638297872340425No Hit
CCCCTAAACAGCGAAAAAGCCCAGCAGAAGTGCTGGGCTTTTTGCTGTCT11.0638297872340425No Hit
GATTGAGCCACTGCACTCCAGCCTGGGCGACAGAGGGAGACTCCGTCTCA11.0638297872340425No Hit
CAACCCATGAGCATAGAGTGCTTTTCCATTTATTTGTGTCATCTATGATC11.0638297872340425No Hit
ACACACGTGAGCTATTCCACCCAGTCTCATGACTTGAGATGGAAAAAATA11.0638297872340425No Hit
ATATAATCCAACGAAAATCAAACATAAGGCAGAACTAACTGCAAGCAGTC11.0638297872340425No Hit
TGTGTTGGGAGTTCCTGGCATAGAGAATGTCACAAAGATATGTTTTCAAT11.0638297872340425No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT11.0638297872340425No Hit
CCATTGGAGTCCATTTGATTGTTCCATTCGATTCCATTCGATTCCTTTCG11.0638297872340425No Hit
GTGTGTGCCTGTGTGTATGTAATCAAATACATTGTTGCTGTTATTATTTT11.0638297872340425No Hit
GGGCCTGAAAGCGCTCCAAATGTCCACTTCCAGATAGTACAAAAGGAGTG11.0638297872340425No Hit
ATAATGGCCTATATTCAAGTTCAGAGGCTCTTTTTTTTGTTGTTGTTTTG11.0638297872340425No Hit
ATTGTAACCCCCCTTCCTCTCAACAGCTGCAACTTCTGGAGCCATCCAAT11.0638297872340425No Hit
ATCCAAATGTCCACATCCAGATACTACAAAAAGAGTGTTTCAAACCTGCT11.0638297872340425No Hit
ATCATTCCCCCCTCTGAAGAGGTATATCTAACTGCTGTTAGTGTAGGGAT11.0638297872340425No Hit
ATATATCCTCTCACTCTCTGGGACACCATAGCCACTGCCCCCTCCCCTGG11.0638297872340425No Hit
ACTGTGGCCCTCCAGTTTGGGTAGGGGCTTGTGGAGGTCAGATGATGAAT11.0638297872340425No Hit
GGTATGGGTCATATCACATGGCACTTTGAGTTACATGTAGTATCACTTAC11.0638297872340425No Hit
GAATCATCATTGAATGCAATCACATGGAATCATCACAGAATGGAATCGTA11.0638297872340425No Hit
GTTCATGATGAAGCGCCCGAGCCGGCCATTGCCGTCCATGTACGGGTGAA11.0638297872340425No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)