FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003674155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCACCACGTCGCCGCGCGCCAGCGGCGTGCCTTCAGTGATCTTGG314.285714285714285No Hit
ACGCAGAACGGGTCGCTCATCTTCCCCCGCGGCATCGTGGTCACGCCCGG29.523809523809524No Hit
GGGAAGAAGCGCTGGTACTTGTCGGCCGCGATCATCACTGCCTTGTAGCC29.523809523809524No Hit
CATTTAACATTCCCGGTTGAAGGTGTGGTTGTGAGGGGTTTATTTTTTTA14.761904761904762No Hit
ATTCTAGCCTGTGTCGAATGCAGACAACGTAATTATACAACTAAAAAAAA14.761904761904762No Hit
TTCCCAGGGCTTCCGTCTATTTTTTCATAGGCGTTCACTAAATCATTTGT14.761904761904762No Hit
CGTTGAATTAAAGCGGTTTTTAATTCTAAATCATTGCGACGACGGCGTGG14.761904761904762No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTTGACCACCAGGTAAAGGCCACGGGACAGGTGTGCGATTTCATTGA14.761904761904762No Hit
CCTTCGCGGCGATCGCGGCCAGCGGCGACGTCAACGTCAACGCGATCGCG14.761904761904762No Hit
GGCACGGCTCTGCAAAAGCTTGATCGTCGGTTCAAATCCGTCTACCGCCT14.761904761904762No Hit
GTCGACCAGCAGGGCAAGATCAGCACCGCCCGCGTGCTCGGCCTGCGCCG14.761904761904762No Hit
TCGTAGGGCAGGTCCTTGTAGAGCGCCTCGCCGATGGCGATCGGCGCGGC14.761904761904762No Hit
GCCGATAGACAGATTCATGTTGCCCGATACTTTAGGTATATTAAATCCGG14.761904761904762No Hit
GTACATGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG14.761904761904762No Hit
ATAATGGATTATAACCGCCTACCTCCATTTTTAATTGATCATTTGCGGTG14.761904761904762No Hit
GCCTACGGCGCCTCGGTGCCGAAGCGTTCGGCCTTGATGGTCTGCTCGCG14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)