FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003674156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTGGCTCGACTGGACCGCCGGCCTGTTCCCGGTGATCCTGGTGGTGT314.285714285714285No Hit
CCGCACACCCACCGAGGCCGAGACCGCGCTGCTGAAGTCCGGCGCCGTCG29.523809523809524No Hit
GTATTCCATAAAATGCGTTTGGCCTGCCCGGGTTGTACACCATAATTGTA14.761904761904762No Hit
CATCGGCGTGATGTTGATGTCGTCATAGGGTTTGGAATCGGCCTGGATCT14.761904761904762No Hit
CCCGGGCAACGTTTTGAAAAAACTTCCCCGTTTTACCGACAATGCTTGCC14.761904761904762No Hit
GTGCCGTCGACGTCGACGGCGATGCCCTCGGGCTTGGCGCCCACAGAGCC14.761904761904762No Hit
AATTTAAAGCGCTTCAACACGAAACAGAAAATGGCTTGTTAGATGAGAAA14.761904761904762No Hit
TCCCTGGAGCGTGCCTTCGCTCGCGCAGCCCTGGCCGCAGCGGCCGATCC14.761904761904762No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14.761904761904762Clontech Universal Primer Mix Long (96% over 26bp)
CCTAAAAAAGCGCCCGACAAGGGCGCTTTTGTTTTTGACAATCTATCGGA14.761904761904762No Hit
GTGCCGTCGACGTCGACGGCGATGCCCTCGGGCTTGGCGCCCAGTGAGCC14.761904761904762No Hit
CTAGAGGCTTTTGTCACCATGCGTGACGAAGCTTTTTTCAGTTGATTTCA14.761904761904762No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG14.761904761904762No Hit
GCGTCATTCCGATGAGTTCATGCGTGTTCATACGGCGGCCACGTCCAGGT14.761904761904762No Hit
GACCAACACCATGCGCACCGGGTGACGATACTGTCTGACGACCGCGACGC14.761904761904762No Hit
AACGGTGATTGAGCAAATTGATGCATTGGGTAAATATTCTTTATTTCCGA14.761904761904762No Hit
GTCTTCGGCAATATTGGTTTGGAGTTTTAGTTGATCGTGGATCACCGCCA14.761904761904762No Hit
GGCCAGCAGCATCACCAGCGAGCGCGGCTACAACGTCGTGATCCACGAGT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)