FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGCAGGCCGGCGAGATCGCCACCCGCCGCCAGGCCCGCCAGGCCGC56.024096385542169No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44.819277108433735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAACAGATAGCCGTTGCCCACAAAGAAGAACGGCTCGTAGTAGACGG33.614457831325301No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT33.614457831325301No Hit
CTCCTGCACCTCGCCGGCCCGAGTCCAGTGCACATCAATTTTCCCTGGGT22.4096385542168677No Hit
CACAGTGACCAACCCAAAGAGGATCGCCAAGGCCGTGAACGAGAAGTCCT22.4096385542168677No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22.4096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
TGCCTGGATTGGCAGGGCGACGCGAGCTTTGCGCTGTGCCGCGATCCGGC22.4096385542168677No Hit
GTGTTGCACAAGTACGTTTTGTGACAGGCAATAAAATTTTAAGAATTCTT22.4096385542168677No Hit
AGGCCGACCAGACCACGCACTTCCTGCAGATCTGGATCCTGCCGAAGGAG22.4096385542168677No Hit
CCCACAATCACCACGACGATGAGGACCACCACCACCACCACGATAAGAAG22.4096385542168677No Hit
GTGCGCCTGCGTGGAACGCCCGCAAGGTGAGGTCGAGCAGGAACTGGCGC22.4096385542168677No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA22.4096385542168677No Hit
GGCCAGGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGA22.4096385542168677No Hit
ACAAACTCCTCTTGTTAGACACTCAACAGGAGGCAGGGATGCTGCTTAGG22.4096385542168677No Hit
GTTGACATCTGACGACGTGAAGGAGCAGATTTACAAACTGGCCAAGAAGG22.4096385542168677No Hit
GGAGTAAGGCCCTTCTTGGCCAGTTTGTAAATCTGCTCCTTCACGTCGTC22.4096385542168677No Hit
GACATGTTCCGCGAGCCGGGCTACGTGCGGCCGCGCGCGGGGGTGGCGCT22.4096385542168677No Hit
GTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACAT11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACA11.2048192771084338No Hit
GTAATTTCCAACTCTAGTTTGTCTTTCTCAGTTTGTAAAATGGCAATGGT11.2048192771084338No Hit
GCTGGAAGCCACACTCCACGACTTTAGACATCAAATCCTGCAGACAAGGG11.2048192771084338No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCATGTCAC11.2048192771084338No Hit
GGCCTTCTTCGCGGGCTCCACCGGCAACATCTACCGCCAGTTCTCGGCGG11.2048192771084338No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG11.2048192771084338No Hit
GTCAATAGGTTGCTGCATATCGTTGGATATGGTGATTTCCAGATGAAACA11.2048192771084338No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGGGCG11.2048192771084338No Hit
GAGTGCAATGGCATGATCTCAGCTCACTGCAATCTCTGCCTCCTGGGTTC11.2048192771084338No Hit
CATCACCTAGGTATTAAGCCCAGCATTCATTAGCTATTCTTCCTGATGCT11.2048192771084338No Hit
GTGTGGGAAATCCTGCGTTTCCCCTGTGAAAGCTTGATTCCTGCCATATG11.2048192771084338No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG11.2048192771084338No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA11.2048192771084338No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT11.2048192771084338No Hit
CTCAAGAACCAGCAGCGCCAGGAGTTCGTCATCGGCGGCTACACCGCGCC11.2048192771084338No Hit
GCCCACACCTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAACT11.2048192771084338No Hit
GTTCCAGTGTCCCTATCTTGAAGCAGCTGCATGGCCTCTGGCAGGGAAAT11.2048192771084338No Hit
CTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTC11.2048192771084338No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC11.2048192771084338No Hit
ATCCTGAGAGATTCACATGGTGTTGCACAAGTACGTTTTGTGACAGGCAA11.2048192771084338No Hit
TTATTGATGTGCTCAACAGCCTTTGAGACACCCTTCCCCATATAGCGAGT11.2048192771084338No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA11.2048192771084338No Hit
ACTTCGACCGGCCCGGCACCTCCAACGGCCCGACCGAGGCCATCAACGGC11.2048192771084338No Hit
GAGATTCACATGGTGTTGCACAAGTACGTTTTGTGACAGGCAATAAAATT11.2048192771084338No Hit
CCCATGAGCAGGGCTCCCACAATCACCACGACGATGAGGACCACCACCAC11.2048192771084338No Hit
GACCAGCTCGACGTGCTCATCGCACAGGGCCCGCCGCGCTCGCCGCTGAA11.2048192771084338No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTG11.2048192771084338No Hit
GTATAACTTCTATGCCAGCACAGTGCTTAATGAATATCTGTTGATTTGTT11.2048192771084338No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAACCAGGTGC11.2048192771084338No Hit
CCACAAATTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATG11.2048192771084338No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT11.2048192771084338No Hit
GAACAGTAAATGATTATTAGAGAGAAGGAATGGACCAAGGAGACAGAAAT11.2048192771084338No Hit
CTGCAGGTCCTTGCGCTCGCCGATGCGGATGCGCTTGTCGTTGAGCGTCG11.2048192771084338No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.2048192771084338No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC11.2048192771084338No Hit
ACTCGGGGCTCTGGTTCCAGTACCAGCTGTCGGTACCGAACAGCTTTTCC11.2048192771084338No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGCGGTACAGGGGGACCCCCTTCTCAACGGCACCAGCTTTGCAGACGG11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)