FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003674160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAAACCCCTAGATCGGGGCGCCCGGCAGCCTACAGCCCGTGTTCCCG56.024096385542169No Hit
TGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCC33.614457831325301No Hit
CTGTACGGCAGCCTGATCGACATCCTCAACCGCGCAGCACCGCTCATGCT33.614457831325301No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22.4096385542168677Clontech SMART CDS Primer II A (96% over 26bp)
CTTTCGAACAGCAACTGCTTTCTTAATTAAATGGTAGAGATCTTCAGGAA22.4096385542168677No Hit
GGAGAGCTTCTGGCCGTTCACTTCGAGCTCGCCGCGCACCAGGTGCACGT22.4096385542168677No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG22.4096385542168677No Hit
GCCCACAGCTTACTTGGCCAAGGGGTTTCTGAAGTTCCTGCCGGCAAACT22.4096385542168677No Hit
GGCTGGTGCTGCATCAGGGCCAGGGCCACGTCGCGGTGCAGCAGCCGGCG22.4096385542168677No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTGTT22.4096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA22.4096385542168677No Hit
CATGGGGAAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGC22.4096385542168677No Hit
CCTCAGTGGGCCAGGTTTGCTCTCCCTCCATTGGATCTGGGATAACCTCT22.4096385542168677No Hit
GTCTTATAATATCGAGCCAAACGGTGAATCCGGCTCTCTATTAGAATCAG22.4096385542168677No Hit
ATCGCCCCCTCGGCCGATGCATGCAGCACCGCCATCTGGGCCGAATCGGG22.4096385542168677No Hit
GCCCAGATAACCAAGCAGAAGTGGGAGGCAGAACCAGTCTACGTGCAGCG22.4096385542168677No Hit
TCCTGGACCGCAAGGCCATCTACGCCACGGCGCGCATGCATGCGCTGCTG22.4096385542168677No Hit
TATAATATCGAGCCAAACGGTGAAACCGGCTCTCTATTAGAATCAGACGG11.2048192771084338No Hit
GTGCATGACTTCTGAATTTAGACTCACCAGGTTTACATCTGAGTTGACCA11.2048192771084338No Hit
CCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCG11.2048192771084338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAGACGGCCGTGCGCCTGCTGCTGCCCGGGGAGTTGGCCAAGCACGC11.2048192771084338No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGACCACGGTCAGG11.2048192771084338No Hit
GCACCCTCCGCTTCCTCTCCTAGGTGACGAGACCCAGTGGCTAGAAGTTC11.2048192771084338No Hit
GGCATATGTGTTGTACTTCTTCACGAAGCTTGCAATGGCCTCATTTTCCT11.2048192771084338No Hit
ATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGG11.2048192771084338No Hit
GTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGG11.2048192771084338No Hit
GGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGC11.2048192771084338No Hit
TACCCGTAGGGGCTACCACAGACCCGGAGGCGTCCTAGATGAAGTAGAGA11.2048192771084338No Hit
GAAGTGCCCAGTGACTTATGGCCAATGTTTGATGCTTAACCCCCCCAATT11.2048192771084338No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC11.2048192771084338No Hit
GGGCGTCATGGTGTCTCATCGTTCGGGGGAGACTGAAGATACCTTCATCG11.2048192771084338No Hit
GAGCTGGAAGCCATTATCCTCAGCAAACTAATGCAGGAACAGACAACCAA11.2048192771084338No Hit
TGTCAGCATTATTACAAGAGACCAAAGAGTTAAAGAATGAAATTAAAGCT11.2048192771084338No Hit
GCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
GGGTGTAGGTGAACACCACCGCGGCGATGATCGCCACGTAGATCACGAGG11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG11.2048192771084338No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA11.2048192771084338No Hit
GCACGGCATTCCGCATTTCTGCGTGTCGATCGCGCTGGTCGAGAACGGCG11.2048192771084338No Hit
GTGTAGTAGCGGTCTGAAGCCGCCGGTCTCGAGCAGCGCTCTGGCGATGT11.2048192771084338No Hit
GGAAGCGCTTGTGGTAGCCGTCGTAGGTGAACATGCCCGGCACGCCCTCG11.2048192771084338No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA11.2048192771084338No Hit
ATTTATGCGACCAGGGCAGAGGCTGTAGATGATTAATATTTACAATTGGG11.2048192771084338No Hit
TTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC11.2048192771084338No Hit
CCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCG11.2048192771084338No Hit
GTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAG11.2048192771084338No Hit
CGGGCAAGGGCCGTCTGGCACTGAAAACCCTAAGCAAGAAGGTGAGAAAA11.2048192771084338No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT11.2048192771084338No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTTTGCGTTGATAA11.2048192771084338No Hit
CTCTATTAGAATCAGACGGAATTTAGCATCCTTATCCTTTCTGTTCCTCT11.2048192771084338No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
AGGCTGTTGAGCACATCAATAAAACTATTGCGCCTGCCCTGGTTAGCAAG11.2048192771084338No Hit
TTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCA11.2048192771084338No Hit
CCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGC11.2048192771084338No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT11.2048192771084338No Hit
CTCCTTCCCCACCACTTTGGCTGCCACCATCCAAAATCCATTCAGCTTGG11.2048192771084338No Hit
TATAATATCGAGCCAATCGGTGAATCCGGCTCTCTATTAGAATCAGACGG11.2048192771084338No Hit
CGCCGTGGGCGCGGGGGGGGCCCGGGGAGGGGGGGGCCGGGCGGGGGTGG11.2048192771084338No Hit
GGCGCACGCGATGCTCGCGCGTCCATTCGCCGAAGGAAACCTCGGCCACC11.2048192771084338No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)