FastQCFastQC Report
Tue 10 May 2022
EGAF00003674686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240412
Sequences flagged as poor quality0
Sequence length146
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4700.19549772889872388No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4080.16970866678867944No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4050.16846080894464502No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4020.16721295110061063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3580.14891103605477263No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3550.14766317821073824No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC3160.13144102623829093No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2900.1206262582566594No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT2790.1160507794951999No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC2780.11563482688052176No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2630.10939553766034973No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2630.10939553766034973No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT2480.10315624844017769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)