FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003674715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003674715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA313.043478260869565No Hit
AGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGAACCATATCAACA28.695652173913043No Hit
GTGGTATCAACGCAGAGTACATGGGGGCTGCATCGCGCGTTCGGCGCGCG28.695652173913043No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCT14.3478260869565215No Hit
GCAAATAAACAGTTTAGGAATGCAGCAACTGACATTTCTAAAGTACAAAA14.3478260869565215No Hit
ATTCTCTCCAAGAGATCGCGCGGCCCGCTTTTCGAGTTTTTACGGGTAAG14.3478260869565215No Hit
GTCTTTCTGTGTAAATTTTAGACTCAGTTTGTCAAGATCTACAAAGTCGT14.3478260869565215No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC14.3478260869565215No Hit
ACTATGTACTGGTTGTACCTGCTGCTGGCGCTGGGCTGTTTCGCCTTCGC14.3478260869565215No Hit
CCCTGGTCCGCACCAGTTCTAAGTCGGGGGGGAGGGGCCCGCCCCGGGGG14.3478260869565215No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG14.3478260869565215No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA14.3478260869565215No Hit
CACCCGGAAGAGGGCGTCTCGGCAGCCGAGGTCATCATGACCCAGCTTCA14.3478260869565215No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG14.3478260869565215No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG14.3478260869565215No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTTAA14.3478260869565215No Hit
GTACAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT14.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATTCATCGCCTCGACCGAGGCCCGCAAGCTGCACGTCCTCGCCAAGGA14.3478260869565215No Hit
CCCTGGTCCGCACCAGTTCTAAGTCGGGTGGGTGGCGCCGGCCCAGGCGC14.3478260869565215No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)